Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,267,824	A→C	L237F (TTA→TTC)	tyrB →	tyrosine aminotransferase, tyrosine‑repressible, PLP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,267,824	0	A	C	100.0%	296.4 / NA	75	L237F (TTA→TTC)	tyrB	tyrosine aminotransferase, tyrosine‑repressible, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (42/33);  total (42/33)

TCTCACTAATGATCAGTGGGATGCGGTGATTGAAATTCTCAAAGCCCGCGAGCTTATTCCATTCCTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTACCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAATTGAAAGCAACAGTTCGCCGCAACTACTCC  >  NC_000913/4267680‑4267968                                                                                                                                                 |                                                                                                                                                 tctcACTAATGATCAGTGGGATGCGGTGATTGAAATTCTCAAAGCCCGCGAGCTTATTCCATTCCTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGc                                                                                                                                             >  1:712703/1‑150 (MQ=255)    cACTAATGATCAGTGGGATGCGGTGATTGAAATTCTCAAAGCCCGCGAGCTTATTCCATTCCTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGctct                                                                                                                                          <  2:483986/150‑1 (MQ=255)    cACTAATGATCAGTGGGATGCGGTGATTGAAATTCTCAAAGCCCGCGAGCTTATTCCATTCCTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGct                                                                                                                                            <  2:177106/148‑1 (MQ=255)    cACTAATGATCAGTGGGATGCGGTGATTGAAATTCTCAAAGCCCGCGAGCTTATTCCATTCCTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGc                                                                                                                                             <  1:658332/147‑1 (MQ=255)    cACTAATGATCAGTGGGATGCGGTGATTGAAATTCTCAAAGCCCGCGAGCTTATTCCATTCCTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTg                                                                                                                                         <  1:933382/151‑1 (MQ=255)    cACTAATGATCAGTGGGATGCGGTGATTGAAATTCTCAAAGCCCGCGAGCTTATTCCATTCCTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTg                                                                                                                                         >  2:459733/1‑151 (MQ=255)      cTAATGATCAGTGGGATGCGGTGATTGAAATTCTCAAAGCCCGCGAGCTTATTCCATTCCTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTgg                                                                                                                                        >  2:344761/1‑150 (MQ=255)        aaTGATCAGTGGGATGCGGTGATTGAAATTCTCAAAGCCCGCGAGCTTATTCCATTCCTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGt                                                                                                                                       <  1:713171/149‑1 (MQ=255)        aaTGATCAGTGGGATGCGGTGATTGAAATTCTCAAAGCCCGCGAGCTTATTCCATTCCTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGt                                                                                                                                       <  2:839510/149‑1 (MQ=255)        aaTGATCAGTGGGATGCGGTGATTGAAATTCTCAAAGCCCGCGAGCTTATTCCATTCCTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTg                                                                                                                                      >  2:171506/1‑150 (MQ=255)             tCAGTGGGATGCGGTGATTGAAATTCTCAAAGCCCGCGAGCTTATTCCATTCCTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAAt                                                                                                                                >  1:152902/1‑151 (MQ=255)               aGTGGGATGCGGTGATTGAAATTCTCAAAGCCCGCGAGCTTATTCCATTCCTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTc                                                                                                                              <  1:83934/151‑1 (MQ=255)               aGTGGGATGCGGTGATTGAAATTCTCAAAGCCCGCGAGCTTATTCCATTCCTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTc                                                                                                                              <  1:781220/151‑1 (MQ=255)                gTGGGATGCGGTGATTGAAATTCTCAAAGCCCGCGAGCTTATTCCATTCCTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCg                                                                                                                             <  1:1112950/151‑1 (MQ=255)                     aTGCGGTGATTGAAATTCTCAAAGCCCGCGAGCTTATTCCATTCCTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTtctc                                                                                                                        >  1:297015/1‑151 (MQ=255)                        cGGTGATTGAAATTCTCAAAGCCCGCGAGCTTATTCCATTCCTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGa                                                                                                                      >  2:115511/1‑150 (MQ=255)                           tGATTGAAATTCTCAAAGCCCGCGAGCTTATTCCATTCCTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAAt                                                                                                                  >  1:352459/1‑151 (MQ=255)                                gAAATTCTCAAAGCCCGCGAGCTTATTCCATTCCTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTtct                                                                                                             <  2:592796/151‑1 (MQ=255)                                      ctcAAAGCCCGCGAGCTTATTCCATTCCTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCttt                                                                                                       >  1:111983/1‑151 (MQ=255)                                        cAAAGCCCGCGAGCTTATTCCATTCCTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTa                                                                                                      <  2:1142796/150‑1 (MQ=255)                                        cAAAGCCCGCGAGCTTATTCCATTCCTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTATCCCTTTAc                                                                                                     >  1:15204/1‑151 (MQ=255)                                          aaGCCCGCGAGCTTATTCCATTCCTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACgg                                                                                                   >  1:583991/1‑151 (MQ=255)                                          aaGCCCGCGAGCTTATTCCATTCCTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACgg                                                                                                   <  2:134481/151‑1 (MQ=255)                                          aaGCCCGCGAGCTTATTCCATTCCTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACgg                                                                                                   <  2:728001/151‑1 (MQ=255)                                           aGCCCGCGAGCTTATTCCATTCCTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGc                                                                                                  >  2:486558/1‑151 (MQ=255)                                            gCCCGCGAGCTTATTCCATTCCTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGc                                                                                                  >  1:275125/1‑150 (MQ=255)                                               cgcgAGCTTATTCCATTCCTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAgc                                                                                              >  1:238063/1‑151 (MQ=255)                                               cgcgAGCTTATTCCATTCCTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAgc                                                                                              <  2:373234/151‑1 (MQ=255)                                                gcgAGCTTATTCCATTCCTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAgcg                                                                                             >  2:708020/1‑151 (MQ=255)                                                 cgAGCTTATTCCATTCCTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTACCTTTACGGCGAgcgc                                                                                            >  1:1010348/1‑151 (MQ=255)                                                       tATTCCATTCCTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTcg                                                                                        <  1:695778/149‑1 (MQ=255)                                                       tATTCCATTCCTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTcg                                                                                        <  2:1093254/149‑1 (MQ=255)                                                        attccattccTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTcggcg                                                                                     >  1:64501/1‑151 (MQ=255)                                                        attccattccTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTcggcg                                                                                     <  1:43242/151‑1 (MQ=255)                                                         ttccattccTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTcg                                                                                        <  2:368980/147‑1 (MQ=255)                                                          tccattccTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTcggcgg                                                                                    <  1:460532/150‑1 (MQ=255)                                                           ccattccTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGAc                                                                                  >  2:586775/1‑151 (MQ=255)                                                               tccTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTc                                                                              >  1:1202275/1‑151 (MQ=255)                                                                     atatTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTAt                                                                        >  2:195604/1‑151 (MQ=255)                                                                           ccTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTAtgtgtg                                                                   >  2:892551/1‑150 (MQ=255)                                                                           ccTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGAACTTTCTGTTAtgtgtg                                                                   >  1:139868/1‑150 (MQ=255)                                                                              aTCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGaa                                                                 <  1:914485/149‑1 (MQ=255)                                                                                cAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATg                                                             <  2:902314/151‑1 (MQ=255)                                                                                 aaGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGc                                                            >  2:1130196/1‑151 (MQ=255)                                                                                 aaGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGc                                                            <  1:115504/151‑1 (MQ=255)                                                                                          gTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGCTTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCg                                                    >  1:230063/1‑150 (MQ=255)                                                                                              cGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGc                                                   >  1:560485/1‑147 (MQ=255)                                                                                              cGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGc                                               <  2:897270/151‑1 (MQ=255)                                                                                               ggTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGcc                                              >  2:1052500/1‑151 (MQ=255)                                                                                               ggTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGcc                                              >  2:455306/1‑151 (MQ=255)                                                                                               ggTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGcc                                              <  2:323110/151‑1 (MQ=255)                                                                                                 tATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCcg                                             <  1:997972/150‑1 (MQ=255)                                                                                                  aTGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCcgcg                                           >  2:828918/1‑151 (MQ=255)                                                                                                    ggAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGt                                          >  2:1100490/1‑150 (MQ=255)                                                                                                     gAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTAc                                        >  1:1054771/1‑151 (MQ=255)                                                                                                       agagGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTg                                      >  2:1049644/1‑151 (MQ=255)                                                                                                        gagGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTgg                                     >  1:861651/1‑151 (MQ=255)                                                                                                             tGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCa                                 >  1:463849/1‑150 (MQ=255)                                                                                                               ccTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAAtt                              <  1:1161655/151‑1 (MQ=255)                                                                                                               ccTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAAtt                              <  1:130673/151‑1 (MQ=255)                                                                                                                cTACGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAATTg                             >  1:1182794/1‑151 (MQ=255)                                                                                                                   cGCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAATTGaaa                          <  1:101142/151‑1 (MQ=255)                                                                                                                    gCTATTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAATTGaa                           >  2:59067/1‑149 (MQ=255)                                                                                                                       aTTCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAATTGAAAGCaa                      <  2:951685/151‑1 (MQ=255)                                                                                                                         tCGCGCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAATTGAAAGCAAc                     >  1:564750/1‑150 (MQ=255)                                                                                                                          cgcgCCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAATTGAAAGCAACa                    <  2:46891/150‑1 (MQ=255)                                                                                                                            cgcCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAATTGAAAGCAACAGtt                 >  2:131577/1‑151 (MQ=255)                                                                                                                             gcCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTGGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAATTGAAAGCAACAGTTc                >  1:112852/1‑151 (MQ=255)                                                                                                                             gcCATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAATTGAAAGCAACAGTTc                <  1:1100453/151‑1 (MQ=255)                                                                                                                               cATTGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAATTGAAAGCAACAGTTcgc              >  2:904063/1‑151 (MQ=255)                                                                                                                                 ttGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAATTGAAAGCAACAGTTcgccg            >  2:809681/1‑151 (MQ=255)                                                                                                                                  tGCCAGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAATTGAAAGCAACAGTTcgccg            <  1:563907/150‑1 (MQ=255)                                                                                                                                      aGCGCTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAATTGAAAGCAACAGTTCGCCGCAact       <  2:152913/151‑1 (MQ=255)                                                                                                                                       gcgcTGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAATTGAAAGCAACAGTTCGCCGCAact       >  2:279516/1‑150 (MQ=255)                                                                                                                                           tGGATTCCCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAATTGAAAGCAACAGTTCGCCGCAACTACTcc  >  2:956997/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                                 TCTCACTAATGATCAGTGGGATGCGGTGATTGAAATTCTCAAAGCCCGCGAGCTTATTCCATTCCTCGATATTGCCTATCAAGGATTTGGTGCCGGTATGGAAGAGGATGCCTACGCTATTCGCGCCATTGCCAGCGCTGGATTACCCGCTCTGGTGAGCAATTCGTTCTCGAAAATTTTCTCCCTTTACGGCGAGCGCGTCGGCGGACTTTCTGTTATGTGTGAAGATGCCGAAGCCGCTGGCCGCGTACTGGGGCAATTGAAAGCAACAGTTCGCCGCAACTACTCC  >  NC_000913/4267680‑4267968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑