Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,661,793 C→A L113F (TTG→TTT)  iscR ← isc operon transcriptional repressor; suf operon transcriptional activator; oxidative stress‑ and iron starvation‑inducible; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,661,7930CA100.0% 388.5 / NA 96L113F (TTG→TTT) iscRisc operon transcriptional repressor; suf operon transcriptional activator; oxidative stress‑ and iron starvation‑inducible; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (46/50);  total (46/50)

AGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATA  >  NC_000913/2661646‑2661939
                                                                                                                                                   |                                                                                                                                                  
aGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTaaa                                                                                                                                                  <  1:992398/150‑1 (MQ=255)
  cgcgTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATc                                                                                                                                               >  2:128035/1‑151 (MQ=255)
   gcgTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATc                                                                                                                                               >  1:1002111/1‑150 (MQ=255)
    cgTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCAc                                                                                                                                             <  2:1162822/151‑1 (MQ=255)
     gTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACg                                                                                                                                            >  2:1117351/1‑151 (MQ=255)
        aCTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCca                                                                                                                                         <  2:712468/151‑1 (MQ=255)
           tAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCcac                                                                                                                                        >  2:548943/1‑149 (MQ=255)
            aaCGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAg                                                                                                                                      <  2:516988/150‑1 (MQ=255)
              cGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAgcgc                                                                                                                                   <  2:232657/151‑1 (MQ=255)
               gTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGcgcg                                                                                                                                  <  2:225031/151‑1 (MQ=255)
                tCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGcgcg                                                                                                                                  >  1:699645/1‑150 (MQ=255)
                tCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGt                                                                                                                                 >  2:637120/1‑151 (MQ=255)
                  gATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTgg                                                                                                                               >  1:559955/1‑151 (MQ=255)
                     cgcgTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTc                                                                                                                            >  2:831999/1‑151 (MQ=255)
                        gTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAgg                                                                                                                         >  2:396534/1‑151 (MQ=255)
                        gTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAgg                                                                                                                         >  1:606384/1‑151 (MQ=255)
                            tgtgtgCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCAtt                                                                                                                     >  2:970821/1‑151 (MQ=255)
                               gtgCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTa                                                                                                                   <  2:884851/150‑1 (MQ=255)
                                  cGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATcgc                                                                                                               >  1:188765/1‑151 (MQ=255)
                                        cGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGTGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCt                                                                                                         <  1:345080/151‑1 (MQ=255)
                                        cGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCt                                                                                                         >  2:799787/1‑151 (MQ=255)
                                             cgcgTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAg                                                                                                    <  1:230833/151‑1 (MQ=255)
                                                gtcgtGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAgccg                                                                                                 <  1:195072/151‑1 (MQ=255)
                                                gtcgtGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAgccg                                                                                                 <  1:595179/151‑1 (MQ=255)
                                                    tGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCtt                                                                                             >  2:1062793/1‑151 (MQ=255)
                                                     gAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCtt                                                                                             <  1:1814/150‑1 (MQ=255)
                                                        tATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTAcc                                                                                         >  2:878140/1‑151 (MQ=255)
                                                         aTGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTAccc                                                                                        <  1:1201587/151‑1 (MQ=255)
                                                              gacgacCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACa                                                                                   <  2:351774/151‑1 (MQ=255)
                                                              cacgacCAGCCACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGACACAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCACCCGCCTTTACCCTGAc                                                                                    >  1:636719/2‑150 (MQ=255)
                                                               acgacCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTg                                                                                      <  1:1155786/147‑1 (MQ=255)
                                                                 gacCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACg                                                                                >  1:127810/1‑151 (MQ=255)
                                                                 gacCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACg                                                                                <  2:638173/151‑1 (MQ=255)
                                                                  acCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACgg                                                                               >  1:985985/1‑151 (MQ=255)
                                                                   ccAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACggg                                                                              >  2:23826/1‑151 (MQ=255)
                                                                   ccAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACggg                                                                              >  1:1153771/1‑151 (MQ=255)
                                                                   ccAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACgg                                                                               <  1:290348/150‑1 (MQ=255)
                                                                     aGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTg                                                                            <  2:403854/151‑1 (MQ=255)
                                                                      gACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTgg                                                                           <  1:904957/151‑1 (MQ=255)
                                                                        cacaTCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTCACCCTGACAACGGGTGGc                                                                          >  1:621787/1‑150 (MQ=255)
                                                                          caTCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCAt                                                                        <  1:983597/150‑1 (MQ=255)
                                                                           aTCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCt                                                                      >  1:1094140/1‑151 (MQ=255)
                                                                            tCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCt                                                                      >  1:779560/1‑150 (MQ=255)
                                                                            tCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCt                                                                      >  2:249018/1‑150 (MQ=255)
                                                                                gCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGa                                                                 <  1:132642/151‑1 (MQ=255)
                                                                                   cTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGAtt                                                               <  2:759519/150‑1 (MQ=255)
                                                                                     tCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTc                                                              <  1:128026/149‑1 (MQ=255)
                                                                                     tCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTc                                                              <  1:981784/149‑1 (MQ=255)
                                                                                      ccTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGt                                                            <  2:260848/150‑1 (MQ=255)
                                                                                         ggTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGACGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAAc                                                        <  2:627771/151‑1 (MQ=255)
                                                                                          gTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACg                                                       <  2:280949/151‑1 (MQ=255)
                                                                                             attaACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGgcg                                                    <  2:348170/151‑1 (MQ=255)
                                                                                             attaACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGgcg                                                    >  2:935212/1‑151 (MQ=255)
                                                                                             attaACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGgcg                                                    >  2:917908/1‑151 (MQ=255)
                                                                                                aaCCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCt                                                  <  2:372165/150‑1 (MQ=255)
                                                                                                aaCCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCt                                                  <  1:987777/150‑1 (MQ=255)
                                                                                                aaCCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCt                                                  >  2:1048042/1‑150 (MQ=255)
                                                                                                 aCCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTa                                                 >  1:403789/1‑150 (MQ=255)
                                                                                                 aCCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTa                                                 >  1:511393/1‑150 (MQ=255)
                                                                                                    aGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTa                                             <  1:896577/151‑1 (MQ=255)
                                                                                                       tCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACtt                                          >  2:1177569/1‑151 (MQ=255)
                                                                                                       tCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACt                                           <  2:419032/150‑1 (MQ=255)
                                                                                                        cGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTc                                         >  2:342973/1‑151 (MQ=255)
                                                                                                        cGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTc                                         <  1:1041111/151‑1 (MQ=255)
                                                                                                        cGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTc                                         <  2:1100099/151‑1 (MQ=255)
                                                                                                        cGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCACTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTc                                         >  2:644118/1‑151 (MQ=255)
                                                                                                          ccTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGc                                       >  2:11372/1‑151 (MQ=255)
                                                                                                          ccTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGc                                       >  1:665322/1‑151 (MQ=255)
                                                                                                            tAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGcc                                      >  2:1088512/1‑150 (MQ=255)
                                                                                                             aaaGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGcc                                      <  2:365556/149‑1 (MQ=255)
                                                                                                             aaaGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCaa                                    <  2:1074636/151‑1 (MQ=255)
                                                                                                             aaaGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCaa                                    <  1:255082/151‑1 (MQ=255)
                                                                                                             aaaGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCa                                     >  2:1177083/1‑150 (MQ=255)
                                                                                                              aaGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAAc                                   >  2:306588/1‑151 (MQ=255)
                                                                                                              aaGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAAc                                   >  2:408098/1‑151 (MQ=255)
                                                                                                              aaGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAAc                                   <  1:708064/151‑1 (MQ=255)
                                                                                                               aGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACg                                  <  2:927321/151‑1 (MQ=255)
                                                                                                                gTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACgg                                 >  2:800054/1‑151 (MQ=255)
                                                                                                                gTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACgg                                 <  1:773084/151‑1 (MQ=255)
                                                                                                                  aaTGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGc                                <  2:831770/150‑1 (MQ=255)
                                                                                                                  aaTGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGc                                <  1:1020492/150‑1 (MQ=255)
                                                                                                                   aTGTTGTTGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCg                               >  2:805258/1‑150 (MQ=255)
                                                                                                                     gttgttGAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGAtg                            <  1:137220/151‑1 (MQ=255)
                                                                                                                          tgAGAAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGAtgctgc                        <  1:448344/150‑1 (MQ=255)
                                                                                                                           gagaAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATgctgct                       <  2:454637/150‑1 (MQ=255)
                                                                                                                           gagaAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGctgctg                      <  1:984321/151‑1 (MQ=255)
                                                                                                                            agaAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTgg                     <  2:1169977/151‑1 (MQ=255)
                                                                                                                             gaAAACCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGc                    <  1:161097/151‑1 (MQ=255)
                                                                                                                              aaaaCCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCa                   <  1:336497/151‑1 (MQ=255)
                                                                                                                               aaaCCGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCAt                  >  2:1046696/1‑151 (MQ=255)
                                                                                                                                  ccGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCtt               >  2:222049/1‑151 (MQ=255)
                                                                                                                                   cGGTGAGACGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACCGCGCTAATTACTTCGCCAAAGGCGATGCTGCTGGCATCttt              >  1:748179/1‑151 (MQ=255)
                                                                                                                                         gaCGGTCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTaa        <  2:686005/151‑1 (MQ=255)
                                                                                                                                              tCGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAAc       >  1:1054136/1‑147 (MQ=255)
                                                                                                                                               cGCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATa  <  2:67789/151‑1 (MQ=255)
                                                                                                                                                gCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGCCAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATa  >  1:1192604/1‑150 (MQ=255)
                                                                                                                                                gCTAAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATa  >  2:98526/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATA  >  NC_000913/2661646‑2661939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑