Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,799,867	C→A	R43L (CGA→CTA)	ygaC ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,799,867	0	C	A	100.0%	317.8 / NA	81	R43L (CGA→CTA)	ygaC	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (46/35);  total (46/35)

GGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTCGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAACCGACTTTTTCAAGTACGCGCGCCACCTCGTCTGGTCGTAAATACATATTTGATCCTCGTTATT  >  NC_000913/2799717‑2800011                                                                                                                                                       |                                                                                                                                                 gggATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTc                                                                                                                                                  <  2:75903/151‑1 (MQ=255)    aTACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTagcg                                                                                                                                               >  1:313295/1‑151 (MQ=255)           aaTGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTcgcg                                                                                                                                        >  2:351915/1‑151 (MQ=255)               cTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGAtt                                                                                                                                     >  2:691751/1‑150 (MQ=255)                      cgctcgcCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGa                                                                                                                             <  1:954601/151‑1 (MQ=255)                       gctcgcCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGAc                                                                                                                            >  1:193932/1‑151 (MQ=255)                       gctcgcCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGAc                                                                                                                            >  1:948440/1‑151 (MQ=255)                           gcCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACa                                                                                                                           <  2:1199471/148‑1 (MQ=255)                           gcCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATaa                                                                                                                        <  2:104307/151‑1 (MQ=255)                           gcCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATa                                                                                                                         >  2:100443/1‑150 (MQ=255)                           gcCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATa                                                                                                                         >  1:598573/1‑150 (MQ=255)                             cTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAAtt                                                                                                                      >  2:46855/1‑151 (MQ=255)                                  aaaTAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTcc                                                                                                                  <  2:667511/150‑1 (MQ=255)                                    aTAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCa                                                                                                               <  2:724433/151‑1 (MQ=255)                                      agagCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCAcg                                                                                                             <  1:534216/151‑1 (MQ=255)                                      agagCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCAc                                                                                                              <  1:1018027/150‑1 (MQ=255)                                       gagCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCAcgg                                                                                                            <  2:242302/151‑1 (MQ=255)                                         gCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCAcggc                                                                                                           <  2:1009767/150‑1 (MQ=255)                                          cGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCAcggcgg                                                                                                         >  2:1092169/1‑151 (MQ=255)                                           ggAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGt                                                                                                        <  2:785362/151‑1 (MQ=255)                                            gAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGt                                                                                                        >  2:30918/1‑150 (MQ=255)                                            gAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGt                                                                                                        >  1:211063/1‑150 (MQ=255)                                               aTTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAAcc                                                                                                    >  1:1116249/1‑151 (MQ=255)                                               aTTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAAcc                                                                                                    <  1:700135/151‑1 (MQ=255)                                                  gCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCAt                                                                                                  <  2:723553/150‑1 (MQ=255)                                                  gCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATa                                                                                                 <  1:116605/151‑1 (MQ=255)                                                    tGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACg                                                                                               >  1:352098/1‑151 (MQ=255)                                                      gTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCt                                                                                             >  1:688081/1‑151 (MQ=255)                                                         aTGATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCttt                                                                                           >  2:351049/1‑150 (MQ=255)                                                         aTGATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTc                                                                                          <  2:526805/151‑1 (MQ=255)                                                           gATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCt                                                                                         >  2:1155479/1‑150 (MQ=255)                                                           gATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCt                                                                                         >  2:775081/1‑150 (MQ=255)                                                           gATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTg                                                                                        <  1:651245/151‑1 (MQ=255)                                                           gATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTg                                                                                        >  2:583253/1‑151 (MQ=255)                                                           gATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGATTTCt                                                                                         >  1:695889/1‑150 (MQ=255)                                                              cGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTggg                                                                                      >  2:384109/1‑150 (MQ=255)                                                               gCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTggg                                                                                      >  1:156057/1‑149 (MQ=255)                                                                cAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGtt                                                                                    >  2:914982/1‑150 (MQ=255)                                                                        aaTATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCg                                                                           >  2:119850/1‑151 (MQ=255)                                                                           atCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGAc                                                                         >  2:1043772/1‑150 (MQ=255)                                                                           atCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCAAACGCTTTCTGGGTTACCACATCGAc                                                                         >  2:1138504/1‑150 (MQ=255)                                                                               gAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTa                                                                     >  1:151606/1‑150 (MQ=255)                                                                                 aGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTaaaa                                                                  <  1:30916/151‑1 (MQ=255)                                                                                 aGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTaaaa                                                                  <  1:971645/151‑1 (MQ=255)                                                                                   cGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAAcc                                                                <  2:273884/151‑1 (MQ=255)                                                                                    gggTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAAcc                                                                >  2:925847/1‑150 (MQ=255)                                                                                    gggTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAACCg                                                               >  1:245145/1‑151 (MQ=255)                                                                                          aGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAACCGACtttt                                                         <  2:151200/151‑1 (MQ=255)                                                                                              aGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAACCGACTTTTTCa                                                      >  1:502420/1‑150 (MQ=255)                                                                                                 gtCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAACCGACTTTTTCAAGTa                                                  <  1:119843/151‑1 (MQ=255)                                                                                                 gtCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAACCGACTTTTTCAAGTa                                                  <  1:934002/151‑1 (MQ=255)                                                                                                  tCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGAAAGTAAAACCGACTTTTTAAAGTa                                                  >  1:433458/1‑150 (MQ=255)                                                                                                   cGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAACCGACTTTTTCAAGTAcg                                                >  1:646311/1‑151 (MQ=255)                                                                                                     aaCTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAACCGACTTTTTCAAGTAcgcg                                              >  1:348026/1‑151 (MQ=255)                                                                                                     aaCTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAACCGACTTTTTCAAGTAcgcg                                              <  1:1155417/151‑1 (MQ=255)                                                                                                         gCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAACCGACTTTTTCAAGTAcgcgcg                                            <  1:457219/149‑1 (MQ=255)                                                                                                         gCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAACCGACTTTTTCAAGTACGCGCGcc                                          >  2:389208/1‑151 (MQ=255)                                                                                                           gTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAACCGACTTTTTCAAGTACGCGCGCCAc                                        >  2:651003/1‑151 (MQ=255)                                                                                                                tttAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAACCGACTTTTTCAAGTACGCGCGCCACCTCg                                    >  1:554145/1‑150 (MQ=255)                                                                                                                  tAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAACCGACTTTTTCAAGTACGCGCGCCACCTCGTc                                  <  1:584182/150‑1 (MQ=255)                                                                                                                   aaTGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAACCGACTTTTTCAAGTACGCGCGCCACCTCGTCt                                 <  2:229181/150‑1 (MQ=255)                                                                                                                   aaTGTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAACCGACTTTTTCAAGTACGCGCGCCACCTCGTCTg                                <  1:1097262/151‑1 (MQ=255)                                                                                                                      gTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAACCGACTTTTTCAAGTACGCGCGCCACCTCGTCTGGTc                             <  1:633649/151‑1 (MQ=255)                                                                                                                      gTTGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAACCGACTTTTTCAAGTACGCGCGCCACCTCGTCTGGTc                             >  2:415007/1‑151 (MQ=255)                                                                                                                       ttGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAACCGACTTTTTCAAGTACGCGCGCCACCTCGTCTGGTCg                            >  1:594230/1‑151 (MQ=255)                                                                                                                        tGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAACCGACTTTTTCAAGTACGCGCGCCACCTCGTCTGGTCg                            <  2:541610/150‑1 (MQ=255)                                                                                                                        tGGATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAACCGACTTTTTCAAGTACGCGCGCCACCTCGTCTGGTCg                            >  1:902929/1‑150 (MQ=255)                                                                                                                          gATGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAACCGACTTTTTCAAGTACGCGCGCCACCTCGTCTGGTCGTaa                         <  2:379815/151‑1 (MQ=255)                                                                                                                           aTGAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAACCGACTTTTTCAAGTACGCGCGCCACCTCGTCTGGTCGTaa                         >  1:1193961/1‑150 (MQ=255)                                                                                                                             gAATTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAACCGACTTTTTCAAGTACGCGCGCCACCTCGTCTGGTCGTAAATa                      <  2:523058/151‑1 (MQ=255)                                                                                                                              aaTTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAACCGACTTTTTCAAGTACGCGCGCCACCTCGTCTGGTCGTAAATAc                     <  1:1002070/151‑1 (MQ=255)                                                                                                                               aTTACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAACCGACTTTTTCAAGTACGCGCGCCACCTCGTCTGGTCGTAAATACa                    <  2:169938/151‑1 (MQ=255)                                                                                                                                 tACCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAACCGACTTTTTCAAGTACGCGCGCCACCTCGTCTGGTCGTAAATACat                   <  2:335526/150‑1 (MQ=255)                                                                                                                                  aCCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAACCGACTTTTTCAAGTACGCGCGCCACCTCGTCTGGTCGTAAATACatat                 >  2:1005791/1‑151 (MQ=255)                                                                                                                                  aCCAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAACCGACTTTTTCAAGTACGCGCGCCACCTCGTCTGGTCGTAAATACatat                 >  2:1183089/1‑151 (MQ=255)                                                                                                                                    cAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAACCGACTTTTTCAAGTACGCGCGCCACCTCGTCTGGTCGTAAATACATAttt               >  1:945754/1‑151 (MQ=255)                                                                                                                                    cAGAGCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCCACAGTAAAACCGACTTTTTCAAGTACGCGCGCCACCTCGTCTGGTCGTAAATACATAttt               >  2:873387/1‑151 (MQ=255)                                                                                                                                      gagCGGTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAACCGACTTTTTCAAGTACGCGCGCCACCTCGTCTGGTCGTAAATACATATTTg              >  1:476851/1‑150 (MQ=255)                                                                                                                                          ggTACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAACCGACTTTTTCAAGTACGCGCGCCACCTCGTCTGGTCGTAAATACATATTTGATCCt         <  1:252626/151‑1 (MQ=255)                                                                                                                                            tACGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAACCGACTTTTTCAAGTACGCGCGCCACCTCGTCTGGTCGTAAATACATATTTGATCCTCg       >  2:201257/1‑151 (MQ=255)                                                                                                                                             aCGTCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAACCGACTTTTTCAAGTACGCGCGCCACCTCGTCTGGTCGTAAATACATATTTGATCCTCg       <  2:719512/150‑1 (MQ=255)                                                                                                                                                tCCCATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAACCGACTTTTTCAAGTACGCGCGCCACCTCGTCTGGTCGTAAATACATATTTGATCCTCGttat   >  1:130481/1‑151 (MQ=255)                                                                                                                                                  ccATTAGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAACCGACTTTTTCAAGTACGCGCGCCACCTCGTCTGGTCGTAAATACATATTTGATCCTCGttatt  >  1:568060/1‑150 (MQ=255)                                                                                                                                                       |                                                                                                                                                 GGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAGTTTTAATATCGGAAGCGGGTTCAGCCAGTGTCGAACTGCGTTCTTTTAATGTTGGATGAATTACCAGAGCGGTACGTCCCATTCGCGCTTCGCGATTAACATAGACATAATTTTCCCCACGGCGGTAACCATACGCTTTCTGGGTTACCACATCGACAGTAAAACCGACTTTTTCAAGTACGCGCGCCACCTCGTCTGGTCGTAAATACATATTTGATCCTCGTTATT  >  NC_000913/2799717‑2800011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑