Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,097,581	0	A	G	41.3%	136.3 / 26.6	75	noncoding (72/88 nt)	serX	tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (2/42);  new base G (31/0);  total (33/42)
Fisher's exact test for biased strand distribution p-value = 4.65e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCTCCTTC‑AGCCA‑CTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAA  >  NC_000913/1097436‑1097731                                                                                                                                                   |                                                                                                                                                          cACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTc                                                                                                                                                        <  2:1098460/149‑1 (MQ=11)                 tGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTCGAACCCCCCCTACGtt                                                                                                                                        >  2:203014/1‑149 (MQ=255)                  gTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGtt                                                                                                                                        <  2:161781/148‑1 (MQ=255)                    ccAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTTGGAAcccccccaagttgtc                                                                                                                                      >  1:43810/1‑139 (MQ=11)                      aaCTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGtat                                                                                                                                 <  2:656409/151‑1 (MQ=255)                            cTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATacaca                                                                                                                            <  1:1144703/150‑1 (MQ=255)                              gTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑tt                                                                                                                        <  2:711084/151‑1 (MQ=255)                               tCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGG‑GGGGGGGGGTCGGAACCCCCCCACGGTTCCGTATACACAC‑ctt                                                                                                                       >  2:103428/1‑151 (MQ=11)                                  ccAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCa                                                                                                                    <  1:930080/151‑1 (MQ=255)                                    aGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTTGAACCCCCCCTACCTTTCCCTGTTCACAA‑TTTcc                                                                                                                     >  1:895560/1‑148 (MQ=11)                                       tGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTCGAAACCCCCCTACGTTTCCCTATTCACAC‑CTTccccgcg                                                                                                                >  1:373405/1‑145 (MQ=11)                                        gTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTg                                                                                                              <  1:26718/151‑1 (MQ=255)                                        gTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTg                                                                                                              <  2:950655/151‑1 (MQ=255)                                          cGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCt                                                                                                            <  1:200308/151‑1 (MQ=255)                                             aCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCTCCt                                                                                                         <  1:644601/151‑1 (MQ=255)                                              ccccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCTCCt                                                                                                         <  1:622614/150‑1 (MQ=255)                                                  ggTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTTTAAACCCCCCAACCGTT‑CCGTATACACAC‑TTTCCACGCGTGCTCCtttcgc                                                                                                    >  1:1112865/1‑147 (MQ=14)                                                       gggCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCTAGGCGTGCTCCTTC‑AGCCA‑CTc                                                                                             <  2:363612/151‑1 (MQ=255)                                                       gggCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCTCCTTC‑AGCCA‑CTc                                                                                             <  1:442757/151‑1 (MQ=255)                                                         gCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCTCCTTC‑AGCCA‑CTCgg                                                                                           <  1:532536/151‑1 (MQ=255)                                                           ttCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGG‑GGGGGGGGGTTGAACCCCCCCCACGGTTCCCTATAAACACTTTTCCAGGGGGGCCCCtccgccaccccgg                                                                                             >  1:652717/1‑138 (MQ=2)                                                             ctcATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTTGAACCCCCCCTACGTTTCCGTATACACAC‑TTTCCCGGCGTGCTCCCTC‑AGCCA‑CTc                                                                                             >  2:183325/1‑145 (MQ=17)                                                               cATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGG‑GGGGGGGGGTCGAACCCCCCCTACCTTGTCGTATATACAC‑TCTCCAGGCGTGCTCCTTC‑CTCCC‑CTCGGACAccc                                                                                     >  1:569399/1‑150 (MQ=17)                                                                aTCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAGG‑GGGGGGGGGTTGAAACCCCCCTACCTTTCCGGATACACAC‑TTTCCCGGGGTGGTCCTTA‑CACCA‑CCCGGTCCCCTc                                                                                    >  1:226864/1‑151 (MQ=9)                                                                   cccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑AGGGGGGGTTTGAACCCCCCCTACGTTGCCGTATACACAC‑CTTCCAGGGGTGCTCCCTC‑AGCCA‑CCCGGGCAccccccc                                                                                 >  1:270238/1‑146 (MQ=17)                                                                    ccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCTCCTTC‑AGCCA‑CTCGGACACCTCACCa                                                                                <  1:669544/151‑1 (MQ=255)                                                                       cGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGG‑GGGGGGGGTTTCAAACCCCCATACCGTGCCCTATACACAC‑TTTCCAGGCGTGCTCCTTC‑AGCCA‑CTCGGACACCCCACCcaa                                                                              >  2:847986/1‑150 (MQ=14)                                                                       cGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTTGAACCCCCCAGACGTTGCCGTATATAAAC‑ATTTCAGGCGGGCTCCTTC‑AGCCC‑CCCCGGCACCTCCCCaaa                                                                              >  1:624417/1‑150 (MQ=14)                                                                       cGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCTCCTTC‑AGCCA‑CTCGGACACCTCACCAAAt                                                                             <  2:153192/151‑1 (MQ=255)                                                                       cGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAGG‑GGGGGGGGGTCGGACCCCCCCTACGGTTCCGTATACACAC‑TTTTCACGCGTGCTCCTTC‑AACCA‑CCCCGGCACCTCCCCaa                                                                               >  1:674312/1‑149 (MQ=14)                                                                        ggTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCTCCTTC‑AGCCA‑CTCGGACACCTCACCAAAtt                                                                            <  2:216717/151‑1 (MQ=255)                                                                             gtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCTCCTTC‑AGCCA‑CTCGGACACCTCACCAAATTGTCg                                                                        <  1:1015150/150‑1 (MQ=255)                                                                             gtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCTCCTTC‑AGCCA‑CTCGGACACCTCACCAAATTGTCGt                                                                       <  2:883306/151‑1 (MQ=255)                                                                             gtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAGA‑GGGGGGGGGTTGGAACCCCGCTACGTTGCCCTATACAAAC‑TTTTCAGGCGTGCTTCTTC‑CGCCC‑CCCCGAAAACTCACCCAAATTTCg                                                                        >  1:1039594/1‑150 (MQ=9)                                                                                cAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTCGGAACCCCCCTACCGTTTCGTATACACAC‑CTTCCCGGCGTGGTCCCTC‑AGCCA‑CCCCGCCACCCCCCCCAATTATCGTTcc                                                                    >  2:414510/1‑151 (MQ=9)                                                                                    ataCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAGA‑GGGGGGGGTTTGAACCCCCCATACGTTGCCGTATAAACAC‑TTTCCACGCGGGCTCCTTC‑AGCCA‑CTCGGACACCCCCCCAAAATTTCGTTTCTGt                                                                 >  1:25966/1‑150 (MQ=14)                                                                                      aCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCTCCTTC‑AGCCA‑CTCGGACACCTCACCAAATTGTCGTTCCTGTCt                                                               <  1:739055/150‑1 (MQ=255)                                                                                        gAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑AGGGGGGGGTTGAAACCCCCATACCTTTGCCTATAAACAC‑TTTTCCGGGGGGGTCCTTCTAGCC‑‑CCCCGACACCCCCCCAAATTTTCGtttccttct                                                               >  1:627045/1‑141 (MQ=2)                                                                                         aaaaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCTCCTTC‑AGCCA‑CTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCt                                                           <  2:271923/151‑1 (MQ=255)                                                                                          aaaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTTG‑TGGGGGGGTGTGCACCCCCCATACGTTTCCGTTTACATCC‑CTTTCAGGCGGGCTCCTTT‑CGCCA‑CTCGGAGACCCCACCAAATTATCGTTCCTGTTTTGCt                                                           >  1:1175031/1‑150 (MQ=9)                                                                                          aaaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTTGAACCCCCCATACGTTTCCGTATACACAC‑TTTCCAGGCGGGCTCCTTT‑AGCCA‑CCCGGACCCCCCACCAAAATTTTGTTCCTGTCtt                                                              >  2:372291/1‑147 (MQ=14)                                                                                          aaaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTTGAAACCCCCCCACCTTTTCGTTTAAACAC‑CTTTCAGGGGGGCCTCCTC‑AGCCA‑CCCCGACACCCCCCCCCAATTTCGTTtctttctt                                                              >  1:698832/1‑147 (MQ=2)                                                                                               aaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCTCCTTC‑AGCCA‑CTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACg                                                     <  1:389556/151‑1 (MQ=255)                                                                                               aaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCTCCTTC‑AGCCA‑CTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACg                                                     <  2:332704/151‑1 (MQ=255)                                                                                               aaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGG‑GGGGGGGGTTGGTAACCCCGCCACAGTGCCGCATAAACAC‑TTTTCCCGCGAGCTCCTTC‑TACCC‑CCCCGACACCCCCCCAAATTGTAGTTTCTg                                                                  >  2:949385/1‑138 (MQ=2)                                                                                               aaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGCGA‑GGGGGGGGTTTGAACCCCCCGCACGTTTCCGTATACACAC‑TTTTCCGGCGGGCTCCTTC‑CACCC‑CTCCGACACCCCACCCAATTTTTGGTTCTTTCTTTCTTGaa                                                       >  2:1146478/1‑149 (MQ=2)                                                                                                  cccGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTTGAACCCCCCATACGTTGCCGGATACACAC‑TTTTCCGGCGGGCTCCTTC‑CGCCA‑CCCCGACACCCCCCCAAAATTTTGTTTCTTTCTTTCTGGAAAggg                                                   >  2:981849/1‑150 (MQ=11)                                                                                                    cGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑AGGGGGGGTTTGAACCCCCCCAAATTTTCCGGAAACAAAC‑TTTCCAGGGCGGCCCCTCC‑CGCCC‑CCCGGCACCCCCCCCCAATTTTCTTTCCTTTTTTTTTGGAACgg                                                    >  2:530386/1‑147 (MQ=2)                                                                                                      tccTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCTCCTTC‑AGCCA‑CTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCt                                               <  1:1160493/148‑1 (MQ=255)                                                                                                       aCTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTTGAAACCCCCCTACGTTTCCGTATACACAC‑TTTCCCCGCGGGCTCCTTC‑ATCCA‑CTCGGGCACCCCACCCAAATTTTGGTCCTGTCCTTCTGGGACGGgcgc                                                >  2:400596/1‑148 (MQ=2)                                                                                                        cTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCTCCTTC‑AGCCA‑CTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTaa                                             <  2:213935/150‑1 (MQ=255)                                                                                                          ttCGTACGAGCTCTTCTTTAAATATGGCGGTGG‑GGGGGGGGTTTGGACCCCCCCCACCTTTCCCTATAAACAA‑TTTTCCGGCGGGGTCCTTC‑AGCCC‑CCCCGGCACCTCCCCCAAATTTTGTTCCTGTCTTTCTTGGACGGgcgcccaatt                                          >  1:261874/1‑145 (MQ=9)                                                                                                          ttCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCTCCTTC‑AGCCA‑CTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAAtt                                           <  2:1090214/150‑1 (MQ=255)                                                                                                            cGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCTCCTTC‑AGCCA‑CTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTa                                         <  1:1198890/150‑1 (MQ=255)                                                                                                               aCGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCTCCTTC‑AGCCA‑CTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGa                                     <  2:652750/151‑1 (MQ=255)                                                                                                                 gAGCTCTTCTTTAAATATGGCGGCGA‑GGGGGGGGTTTGTACCCCCCAGACCTTTTCCTATAAACAC‑CTTTCAGGGGGGCTCCCTT‑CCCCA‑CCCCGACACCCCGCCCAAATTTCGGTCCCTGCTTTCTGGAAAAGGAGCTAAATTAgg                                       >  1:986648/1‑147 (MQ=2)                                                                                                                     tcttctTTAAATATGGCGGTGA‑GGGGGGGGTTCGAAACCCCCATAAGGTTCCGTATAAACAC‑CTTTCAGGCGGGCTCCCTT‑ATCCA‑CTCGGACACCCCCCCCAAATTTCGTTCCTGTCTTTCTGGGACGGGCGCCAATTTTGGGGAAACa                                >  2:820981/1‑150 (MQ=9)                                                                                                                      cttcttTAAATATGGCGGTGA‑GGGGGGGGGTCGGACCCCCCCTACGTTGCCGGGTACACAC‑TTTTCCGGCGGGGCCCCTC‑AGCCACCCCGGCAACCCCCCCAAATT‑TCGTTCCCTTCTTTTTGGGAAGGGCGCTAATTTTGGGgaaac                                 >  2:859537/1‑146 (MQ=2)                                                                                                                       ttcttTAAATATGGCGGTGA‑GGGGGGGGTTTGAACCCCCCCTACGTTGCCCTATACACAC‑TTTTCCGGCGGGGTCCCTT‑CGCCC‑CCCGGGCACCTCCCCAAAATTTCGTTCCTGTCTTTCTGGGACGGGCGCTCATTTTGGGgaa                                   >  2:14364/1‑145 (MQ=9)                                                                                                                        tcttTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCTCCTTC‑AGCCA‑CTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATg                              <  2:360633/149‑1 (MQ=255)                                                                                                                        tcttTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCTCCTTC‑AGCCA‑CTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATg                              <  2:485906/149‑1 (MQ=255)                                                                                                                             aaaTATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCTCCTTC‑AGCCA‑CTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAg                       <  2:646020/151‑1 (MQ=255)                                                                                                                              aaTATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCTCCTTC‑AGCCA‑CTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAgg                      <  1:341940/151‑1 (MQ=255)                                                                                                                              aaTATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCTCCTTC‑AGCCA‑CTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAgg                      <  2:235698/151‑1 (MQ=255)                                                                                                                              aaTATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCTCCTTC‑AGCCA‑CTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAgg                      <  2:512289/151‑1 (MQ=255)                                                                                                                               atatGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCTCCTTC‑AGCCA‑CTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGt                     <  2:291871/151‑1 (MQ=255)                                                                                                                                 atGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCTCCTTC‑AGCCA‑CTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTca                   <  2:807264/151‑1 (MQ=255)                                                                                                                                   ggCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCTCCTTC‑AGCCA‑CTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTcaac                 <  2:323513/151‑1 (MQ=255)                                                                                                                                    gCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCTCCTTC‑AGCCA‑CTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTcaaca                <  2:351205/151‑1 (MQ=255)                                                                                                                                    gCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCTCCTTC‑AGCCA‑CTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTcaaca                <  2:921053/151‑1 (MQ=255)                                                                                                                                     cGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCTCCTTC‑AGCCA‑CTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTcaacaa               <  1:1001762/151‑1 (MQ=255)                                                                                                                                      ggTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCTCCTTC‑AGCCA‑CTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTcaacaa               >  1:942205/1‑150 (MQ=255)                                                                                                                                         gA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCTCCTTC‑AGCCA‑CTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACtt           >  1:1169419/1‑151 (MQ=255)                                                                                                                                            gggggggATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCTCCTTC‑AGCCA‑CTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACtttt         <  2:747094/151‑1 (MQ=255)                                                                                                                                             ggggggATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCTCCTTC‑AGCCA‑CTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACttttt        <  1:20560/151‑1 (MQ=255)                                                                                                                                                   aTTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCTCCTTC‑AGCCA‑CTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGaaaaa  <  1:908346/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                          CACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACAC‑TTTCCAGGCGTGCTCCTTC‑AGCCA‑CTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAA  >  NC_000913/1097436‑1097731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑