Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,443,2770AC46.8% 111.5 / 36.1 79V274G (GTA→GGA) secYpreprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (37/5);  new base C (0/37);  total (37/42)
Fisher's exact test for biased strand distribution p-value = 3.08e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAA  >  NC_000913/3443127‑3443422
                                                                                                                                                      |                                                                                                                                                 
gCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTa                                                                                                                                                   <  2:1107129/151‑1 (MQ=255)
 cAGACGCTTAGAGTAGCACATAAAGCGGTTGCCCAGGCTGAAAATACAGCGAAATTGTTGTCAGCCAGTTCCACCCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACATAATAATACTGGAAGCGAAGATTGCCGGGATTAc                                                                                                                                                  <  2:772944/151‑1 (MQ=255)
    aCGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTAcccc                                                                                                                                               >  1:425383/1‑151 (MQ=255)
       cATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGc                                                                                                                                            >  2:1124570/1‑151 (MQ=255)
        aTAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGcc                                                                                                                                           >  1:973941/1‑151 (MQ=255)
        aTAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGcc                                                                                                                                           <  2:648207/151‑1 (MQ=255)
          agagTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCat                                                                                                                                         >  2:767890/1‑151 (MQ=255)
          agagTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCa                                                                                                                                          <  1:398733/150‑1 (MQ=255)
              tAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTc                                                                                                                                     >  1:1011962/1‑151 (MQ=255)
              tAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTc                                                                                                                                     >  2:367216/1‑151 (MQ=255)
                       aaGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGc                                                                                                                            >  2:563646/1‑151 (MQ=255)
                               gCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAAtg                                                                                                                    >  1:581809/1‑151 (MQ=255)
                                cccGGGCTGAAAATACAGCGAAATTTTTGTCAGCCATTCCCACCCAGTCCCGCCCCCGAACCATGACGCGAGGGTCGCCGGAAAAAGAATAATACTGGAAGCAAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAAt                                                                                                                     <  2:828754/149‑1 (MQ=255)
                                cccGGGCTGAAAATACAGCAAATTTGTTGTCAGCCATTCCCAACCGGTCCCCCCCCCAAACCATGACGGGAGGGTCGCCGGGAAAAAAATAATACTGAAAGCGAAGTTTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTaaa                                                                                                                      <  2:410237/148‑1 (MQ=255)
                                cccAGGCTGAAAATACAGCAAATTTGTTGTCAGCCAGTTCCACCCAGTCCCCCCCCCAAACCATGACGCGAGGGCCGCCGGAAACAAAATAATACGGAAAGCGAAGATTGCGGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAAtgt                                                                                                                   <  2:73485/151‑1 (MQ=255)
                                 ccgggctcaaaaaACAGCAAAATGGTTGTCACCCATTCCCACCCGGTCCCGCCCCCGACCCAGGGCGGGAGGGCCGCGGGAAACAAAATAATACTGGAGGCGAAGATGGCGGGAATTCCCCCCGCCATATTCACTTTCAGCGGTAAAtgtg                                                                                                                  <  2:817963/142‑1 (MQ=255)
                                 ccAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAAtgtg                                                                                                                  >  2:1049679/1‑151 (MQ=255)
                                  cAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGCCGCCGGGAACAGAATAATACTGGATGCGAAGATTGCCGGGATTACCCACGCCATATTCACTGTCAGCGGCAAAtgcgt                                                                                                                 >  1:327494/1‑151 (MQ=255)
                                          aaaaagaGCGAATTTTTTGCCACCCGGTCCCACCCAGCCCCCCCCCCGAACCAGGCCGGGAGGGTCGCGGGAAAAAAAATAAGACTGGAAGCGAAAATGGCGGGGATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGct                                                                                                              <  1:679287/140‑1 (MQ=255)
                                          aaaTCCAGCAAAATTGTTGTCAGCCTGTTCCAACCAGCACCCCCCCCGAACCATGCCGCGATGGTCGCCGGGAACAAAATAATCCTGAAAGCGAAGATGGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGctct                                                                                                            <  2:278706/148‑1 (MQ=255)
                                          aaaTACAGCAAATTTGTTGTCAGCCAGTCCCACCCAGCCCCCCCCCCGAACCAGGACGCGATGGCCGCCGGAAAAAAAATAATACTGGAAGCGAAGATTGCCGGTTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGctct                                                                                                            <  2:1123233/148‑1 (MQ=255)
                                          aaaTACAGCAAAATTGTTGCCAGCCAGTTCCACCCAGTCCCCCCCCCAACCCATGCCGGGATGGCCGCGGGAAAAAAAAAAATACTGGAAGCGAAGATTGCGGGATTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtg                                                                                                           <  2:371824/149‑1 (MQ=255)
                                          aaaTACAGAAAATTTTTTGTCACCCAGTCCAACCCAGTCCCCCCCCCGAACCAGGACGGGAGGGCCGCGGGAAACAGAAAAATCCGGGAAGGGAAGATTGCGGGTTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtgt                                                                                                          <  2:1111794/150‑1 (MQ=255)
                                                aGCAAATTTGTTGTCAGCCATTCCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCGGGGAACAAAAAAATACGGGAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCAt                                                                                                   <  2:650673/151‑1 (MQ=255)
                                                    aatttgttgCCAGCCAGTCCAACCCAGCACCCCCCCCGAACCATGACGCGATGGTCGCCGGAAAAAAAATAATATGGAAAGGGAAGTTGGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgcagc                                                                                                     <  1:563153/142‑1 (MQ=255)
                                                    aaaTTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGac                                                                                               >  2:543541/1‑151 (MQ=255)
                                                     aaTTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGac                                                                                               >  1:1022097/1‑150 (MQ=255)
                                                      aTTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGacac                                                                                             >  2:880719/1‑151 (MQ=255)
                                                          ttgtCAGCCAGTCCCAACCAGTCCCCCCCCCGAACCATGCCGCGATGGTCGCCGGAAACAAAAAAATACTGGAAGCGAAGTTTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgac                                                                                          <  2:246767/150‑1 (MQ=255)
                                                          ttgcaacccAGTACAACCCAGCCCCCCCCCCGAACCACGCCGGGATGGTCGCCGGAAAAAAAATAATCGTGAAAGCGAAGTTGGCGGGAATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacga                                                                                           <  1:726318/142‑1 (MQ=255)
                                                              cAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCt                                                                                     >  2:445246/1‑151 (MQ=255)
                                                               agccgttcccACCCAGCCCCGCCCCCGACCCAGGACGCGATGGTCGCGGGAAAAAAAATAATACGGGAAGCGAAGATTGCGGGTATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgacg                                                                                         <  1:274050/138‑1 (MQ=255)
                                                               aGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCtt                                                                                    >  2:699540/1‑151 (MQ=255)
                                                                gCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTg                                                                                   >  2:723511/1‑151 (MQ=255)
                                                                 ccAGTCCCAACCAGTCCCCCCCCCGAACCATGCCGCGATGGCCGCCGGGAAAAGAAAAATACTGAAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGAcc                                                                                      <  2:903878/147‑1 (MQ=255)
                                                                 caagtccaaaCCACTCCCCCCCCCGAACCAGGCCGGGGGGGTCGCGGGAAAAAGAAAAATAGTGAAAGAAAAGATTGCGGTGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCtt                                                                                    <  1:785950/141‑1 (MQ=255)
                                                                       ccACCCATTCCCCCCCCCAACCCGTGCCGCGATGTTCGCCGGGAAAAGAAAAATCCGGGAAGGGAAAATTGCCGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGAc                                                                              <  2:205798/149‑1 (MQ=255)
                                                                       ccACCCAGTCCCCCCCCCGAACCATGCCGCGATGGCCGCGGGAAACAAAATAATACTGAAAGGGAAGATGGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGAc                                                                              <  1:194101/149‑1 (MQ=255)
                                                                           ccagccccccccccGAACAATGGCGCGGGGGTCGCGGGAAAAAAAATAATACTGAAAGCAAAGTTTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGttt                                                                          <  1:1196942/140‑1 (MQ=255)
                                                                           ccAGTCCCCCCCCCGAACCATGACGCGATGGTCGCCGGGAACAAAATAATACTGGAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGttt                                                                          <  2:842696/149‑1 (MQ=255)
                                                                            cAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTcgc                                                                       >  2:553804/1‑151 (MQ=255)
                                                                             aGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTcgcg                                                                      >  2:20476/1‑151 (MQ=255)
                                                                                 ccccccccGAACCATGACGCGAGGGTCGCCGGAAAAAAAAAAATACTGGAAGCAAAGATTGCGGGATTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGt                                                                  <  2:673100/148‑1 (MQ=255)
                                                                                 ccccccccAAACCATGGCGCGATGGTCGCGGGAAACAAAATAATACTGGAGGCGAAGTTTGCGGGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGt                                                                  <  1:367197/148‑1 (MQ=255)
                                                                                     ccccGAACCAGGACGCGATGGTCGCCGGAAACAAAAAAATACTGGAAGCGAAGTTTGCCGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTAc                                                              <  2:966597/151‑1 (MQ=255)
                                                                                         gAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACcac                                                           >  2:288324/1‑150 (MQ=255)
                                                                                         gAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACcac                                                           >  2:701028/1‑150 (MQ=255)
                                                                                          aaCCAGGACGGGGTGGTCGCGGGGAACAAAATAATATTGGAAGAAAAGATGGCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACcaca                                                          <  1:569524/150‑1 (MQ=255)
                                                                                            ccATGACGTGATGGTCGCCGGAAACAAAATAATAGTGGAAGCAAAGATCGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAAt                                                        <  2:878985/150‑1 (MQ=255)
                                                                                                gACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcggc                                                   >  1:742049/1‑151 (MQ=255)
                                                                                                  cgcgATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGt                                                 >  1:1023959/1‑151 (MQ=255)
                                                                                                   gcgTTGGCCGCCGGAAAAAAAAAAATCCTGGAAGCAAAGTTTGCCGGATTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGtt                                                <  1:652120/151‑1 (MQ=255)
                                                                                                   gcgATGGGCGCCGGAAAAAGAATAATACTGGAAGCGAAGTTTGCCGGGTTTCCCTCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcggcg                                                  <  2:722415/149‑1 (MQ=255)
                                                                                                          tCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACg                                         >  1:433947/1‑151 (MQ=255)
                                                                                                           cGCCGGAAACAAAATAATACTGGAACGAAATATTGCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCa                                           <  1:431624/148‑1 (MQ=255)
                                                                                                               gggAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTca                                     >  2:954566/1‑150 (MQ=255)
                                                                                                               gggAAAAAAAAAATACGGGAAGCGAAGATCGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaa                                    <  2:14145/151‑1 (MQ=255)
                                                                                                               ggaaaCAGAATAATCCTGGAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTca                                     <  1:504527/147‑1 (MQ=255)
                                                                                                                ggAAAAAAATAATAATGAAAAGGAAGATCGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTc                                      <  1:106758/148‑1 (MQ=255)
                                                                                                                gaaaaaaaaaaataTTGGAAGCAAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATGGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaac                                   <  1:767851/141‑1 (MQ=255)
                                                                                                                 gAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGTGGCGTTGACCACGCTcaac                                   >  1:330836/1‑150 (MQ=255)
                                                                                                                     aaaataatCCTGAAAGCGAAATTTGCGGGGATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATa                              <  2:1144311/149‑1 (MQ=255)
                                                                                                                     aaaaaaataCTGGAGGAGAAGTTGGCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATa                              <  1:1073034/146‑1 (MQ=255)
                                                                                                                      gAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATac                             >  2:148586/1‑151 (MQ=255)
                                                                                                                       aataatACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATac                             >  1:395618/1‑150 (MQ=255)
                                                                                                                       aaaaataCTGGAAGCGAAGATTGCGGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATaca                            <  2:1121398/148‑1 (MQ=255)
                                                                                                                        ataataCTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATaca                            >  1:291005/1‑150 (MQ=255)
                                                                                                                         taataCTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaac                          >  1:630382/1‑151 (MQ=255)
                                                                                                                          aataCTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaac                          >  1:401707/1‑150 (MQ=255)
                                                                                                                          aataCTGGAAGAAAAGATTGCCGGAATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaaca                         <  1:1124516/151‑1 (MQ=255)
                                                                                                                           ataCTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaaca                         >  2:658765/1‑150 (MQ=255)
                                                                                                                                  aaGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGt                 >  1:204342/1‑151 (MQ=255)
                                                                                                                                    gCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTc                >  1:460830/1‑150 (MQ=255)
                                                                                                                                     cGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCAc              >  1:598064/1‑151 (MQ=255)
                                                                                                                                       aaGTTTGCGGGAATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACt             <  2:229178/150‑1 (MQ=255)
                                                                                                                                         gatggcggtgATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTg            <  1:341004/140‑1 (MQ=255)
                                                                                                                                         gATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGc           >  2:762799/1‑150 (MQ=255)
                                                                                                                                             gCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaat       >  2:948688/1‑150 (MQ=255)
                                                                                                                                               cGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatact    >  1:139931/1‑151 (MQ=255)
                                                                                                                                                 ggATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatactaa  <  2:226977/151‑1 (MQ=255)
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GCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAA  >  NC_000913/3443127‑3443422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: