Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,434,882	0	T	G	44.3%	107.9 / 13.7	61	Y252S (TAC→TCC)	truA	tRNA pseudouridine(38‑40) synthase
Reads supporting (aligned to +/- strand):  ref base T (4/30);  new base G (27/0);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 2.25e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGA  >  NC_000913/2434740‑2435026                                                                                                                                                |                                                                                                                                                  atatTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGATCATAACGGGCACGGGAAAACAcc                                                                                                                                           >  1:612905/1‑151 (MQ=255) atatTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCAcc                                                                                                                                           <  2:987457/151‑1 (MQ=255)  tatTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGATCATAACGGTCAGGGGAATCCAcc                                                                                                                                           >  2:1070525/1‑150 (MQ=255)  tatTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCTAACCCGTCAGGGGAATCCAcc                                                                                                                                           >  2:280778/1‑150 (MQ=255)            tGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGATCATACCGGGCAGGGGAATCCACCCCGGcc                                                                                                                                     >  1:593720/1‑146 (MQ=255)              ccTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCCGGGGAATCCACCGCGACCAGATa                                                                                                                                >  2:1152039/1‑149 (MQ=255)              ccTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGGAAACCACCGCGACCAGATa                                                                                                                                >  2:966565/1‑149 (MQ=255)                ttGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATAC‑a                                                                                                                             >  2:3564/1‑149 (MQ=255)                  gTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGcc                                                                                                                          <  1:451031/150‑1 (MQ=255)                    aCTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCt                                                                                                                        <  2:380213/150‑1 (MQ=255)                      tCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGATCATACCGGGCAGGGGAAAACACCCCGACCCGATAC‑AACCCCTcc                                                                                                                     >  1:1056720/1‑151 (MQ=255)                       cGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGATCATACCGGTCAGGGGAATCCACCGCGACCCGATAC‑AGCCCTTcc                                                                                                                     >  1:512301/1‑150 (MQ=255)                           aTCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATAA‑AGCCCTTCCGCttt                                                                                                                >  2:847771/1‑151 (MQ=255)                           aTCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGATCATACCGGTCAGGGGAATCCACCCCGCACCGATAC‑CACCCCTCCGCctt                                                                                                                >  1:752947/1‑151 (MQ=255)                             cTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGATCATACCAGTCCGGGGAAACCACCCCGACCAGATAC‑ACCCCCTTCCCttttt                                                                                                              >  1:439882/1‑150 (MQ=255)                               ttCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGATCATACCCGGCAGGGGAATCCACCCCGACCCGATACAACCCCTTCCCCttt                                                                                                                >  1:879141/1‑148 (MQ=255)                               ttCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCtttt                                                                                                               <  2:209669/148‑1 (MQ=255)                                  aGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGGAGAACATAACCGGCAGGGGAAACCCCCGCGACCAGATAA‑AGCCCCTCCCCTTTTGCCGtt                                                                                                         >  2:621844/1‑151 (MQ=255)                                  aGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATAC‑AACCCCTCCGCTTTTTCCGtt                                                                                                         >  2:714268/1‑151 (MQ=255)                                   ggCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTtg                                                                                                        <  2:1038533/151‑1 (MQ=255)                                    gCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGATCATAACGGTCAGGGGAATCCACCCCGACCAGATAC‑AGCCCTTCCCCTTTTGCCGt                                                                                                          >  2:502154/1‑148 (MQ=255)                                         aaCTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTgccg                                                                                                  <  1:232264/151‑1 (MQ=255)                                                     tACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATAACCGGCAGGGGAATCCACCCCGACCAGATAA‑AACCCCTCCCCCTTTTCCCTTGCTGCCCCCCGCGtt                                                                                          >  2:651867/1‑147 (MQ=255)                                                      aCAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCTCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTg                                                                                       <  1:835807/149‑1 (MQ=255)                                                      aCAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTg                                                                                       <  2:989750/149‑1 (MQ=255)                                                      aCAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTg                                                                                       <  1:905772/149‑1 (MQ=255)                                                       cAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTcc                                                                                    <  2:1042146/151‑1 (MQ=255)                                                         gCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATAC‑AACCCTTCCGCTTTTGCCGTTGCTGCCGCCCGCGGTCTGTcc                                                                                    >  2:1148273/1‑149 (MQ=255)                                                          ccTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCttt                                                                                 <  2:693291/151‑1 (MQ=255)                                                          ccTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCttt                                                                                 <  1:679142/151‑1 (MQ=255)                                                           cTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGGAGAACATACCCGGCAGGGGAATCCACCCCGCCCAGATAC‑AGCCCCTCCGCCTTTTCCGTTGCTTCCGCCCGCGTTTTGTCCttt                                                                                 >  2:717142/1‑150 (MQ=255)                                                            tGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTTCCGTTGCTGCCGCCAGCGGTCTGTCCttt                                                                                 >  1:232917/1‑149 (MQ=255)                                                             gAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTg                                                                                <  1:850721/149‑1 (MQ=255)                                                               aaTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAAAAT‑ATAACCGGCAGGGGAATCCAACCCCACCAGATAC‑AGCCCCTCCGCTTTTTCCGTTGCTGCCGCCCGCGTTCTTTCCTTTgcc                                                                              >  2:520848/1‑149 (MQ=255)                                                                   ttCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGGACATTCCCGTCAGGGTAAACCACCGCGCCCAGAAAA‑AAGCCCTCCCCTTTTGTCGTTGCTGGCACCCGCGGTCTTTCCCTTttcgcc                                                                           >  1:373765/1‑148 (MQ=255)                                                                   ttCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGATCAAACCGGTCAGGGGAATCCACCGCGACCAGATAC‑AGCCCCTCCGCTTTTTCCGTTGCTGCCCCCCGCGGTCTGTCCTTTtccgcc                                                                           >  2:835484/1‑148 (MQ=255)                                                                        aGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAActc                                                                   <  1:992466/151‑1 (MQ=255)                                                                         gCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGATCATACCGGGCAGGGGAATACACCGCGACCAGATAC‑AGCCCTTCCCCTTTTGCCGTTGCTGCCGCCCGCGGTCCGTTCTTTTCCGCCCGCAAccc                                                                   >  1:494506/1‑148 (MQ=255)                                                                             tattCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGATCATACCGGTCAGGGGAATCCACCCCGACCAGATAA‑AGCCCCTCCGCCTTTTCCGTTGCTGCCCCCCGCGTTCTTTCCTTTTCCGCCAGCaa                                                                      >  1:998246/1‑143 (MQ=255)                                                                             tattCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCt                                                               <  2:639647/150‑1 (MQ=255)                                                                              attCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGGAATCCACCCCGACCAGAAAA‑AGCCCTTCCGCTTTTTCCGTTGCTGCCCCCACCGTTCTGTCCTTTGCCGCCACCAACTCTGCTAt                                                             >  2:119711/1‑151 (MQ=255)                                                                                   cGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCa                                                          <  2:704451/149‑1 (MQ=255)                                                                                   cGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAg                                                         <  1:1020552/150‑1 (MQ=255)                                                                                            gCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTTAAAATCATACCCGGCAGGGGAATCCACCCCGACCAGATAC‑AACCCCTCCCCTTTTTCCCTTGCTGCCGCCCGCGGTCTTTCCCTTGCCGCCCGCAACCCCGCTATCCAGCTCTCCgg                                                 >  1:210327/1‑149 (MQ=255)                                                                                             ccAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGATCATACCGGGCAGGGGAAACCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCACCGTTCTGTCCTTTTCCGCCAGCAACTCTGCTATCCCGCTCTcc                                                   >  2:39918/1‑146 (MQ=255)                                                                                                     aGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTgg                                       <  1:684562/150‑1 (MQ=255)                                                                                                        gggCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCAc                                   <  1:696098/151‑1 (MQ=255)                                                                                                                 ggCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCa                          <  2:596788/151‑1 (MQ=255)                                                                                                                  gCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCAt                         <  2:802957/151‑1 (MQ=255)                                                                                                                    gg‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCa                       <  2:84455/151‑1 (MQ=255)                                                                                                                     g‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAg                      <  2:514188/151‑1 (MQ=255)                                                                                                                        tttGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAgg                     <  1:1075269/150‑1 (MQ=255)                                                                                                                        tttGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAgg                     <  1:1070468/150‑1 (MQ=255)                                                                                                                          tGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTg                  >  2:82805/1‑151 (MQ=255)                                                                                                                          tGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTg                  >  2:300624/1‑151 (MQ=255)                                                                                                                                  cATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAat          <  2:666237/151‑1 (MQ=255)                                                                                                                                  cATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAat          <  2:660919/151‑1 (MQ=255)                                                                                                                                   aTACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAata         <  1:428929/151‑1 (MQ=255)                                                                                                                                     aCCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATAtt       <  2:1014815/151‑1 (MQ=255)                                                                                                                                          tCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTg   >  2:995467/1‑150 (MQ=255)                                                                                                                                           cAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGa  <  2:378237/150‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                  ATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGA  >  NC_000913/2434740‑2435026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑