Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,042,604 | 0 | A | C | 45.0% | 96.1 / 16.2 | 60 | V182G (GTG→GGG) | yqfA | hemolysin III family HylIII inner membrane protein |
Reads supporting (aligned to +/- strand): ref base A (26/7); new base C (0/27); total (26/34) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 8.34e-11 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGAC‑GTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCG > NC_000913/3042456‑3042748 | gcgcGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGAC‑GTAGAAAATCAcc > 2:693613/1‑151 (MQ=255) gcgcGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGAC‑GTAGAAAATCAcc > 2:474333/1‑151 (MQ=255) cgcgTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGAC‑GTAGAAAATCAccc < 2:774108/151‑1 (MQ=255) tATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGAC‑GTAGAAAATCACCCCGAGc < 1:271024/149‑1 (MQ=255) tATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGAC‑GTAGAAAATCACCCCGAGCg < 1:660531/150‑1 (MQ=255) aTCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGAC‑GTAGAAAATCACCCCGAGCGa < 2:612138/150‑1 (MQ=255) cAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGAC‑GTAGAAAATCACCCCGAGCGAAt < 2:936713/150‑1 (MQ=255) ccTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGAC‑GTAGAAAATCACCCCGAGCGAATAAAcc > 2:233979/1‑151 (MQ=255) tACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGAC‑GTAGAAAATCACCCCGAGCGAATAAACCAc > 1:532124/1‑151 (MQ=255) aCAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGAC‑GTAGAAAATCACCCCGAGCGAATAAACCAcg > 1:482236/1‑151 (MQ=255) cAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGAC‑GTAGAAAATCACCCCGAGCGAATAAACCAcgc > 2:198797/1‑151 (MQ=255) aaaGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGAC‑GTAGAAAATCACCCCGAGCGAATAAACCAcgcc > 1:293336/1‑151 (MQ=255) aaaGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGAC‑GTAGAAAATCACCCCGAGCGAATAAACCAcgcc > 2:197954/1‑151 (MQ=255) cccATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGAC‑GTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGc > 2:746855/1‑151 (MQ=255) aTTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGAC‑GTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGcc > 2:832103/1‑149 (MQ=255) gCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGAC‑GTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAgg > 1:1119129/1‑151 (MQ=255) cccAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGAC‑GTAGAAAATCCCCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTaa < 2:247408/149‑1 (MQ=255) ccaaaTATACAAATAGACCGCCAGAAAGTGGCACCCCCTCCCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGAC‑GTAGAAAAGCCCCCCGAGCGAATAAACCCCGCCGCCTACCGCCAGTAAGGTAAc < 1:359416/147‑1 (MQ=255) ccaaaTATAAAAAAAGACCGCCAAAAGGTGGCACACCCTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAAGGCGTTTGCAGAC‑GTAGAAAATCCCCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACg < 1:184138/148‑1 (MQ=255) ccAATATACAAATAGATCCCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGAC‑GTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGc < 2:383662/151‑1 (MQ=255) tAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGAC‑GTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCcgcg > 2:580584/1‑150 (MQ=255) cGCCAAAAGGGGGCACACACTCCCGCCGAGCACGAAGCCGTGCCAGTGGGCTTGTTTGTAGGGAAGGCGTTTGCAGAC‑GTAAAAAACCCCCCCGAGCAAAAAACCCCCGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCtt < 2:646855/150‑1 (MQ=255) caaGAAAGGGGCACACCCTCCCCCCGAGCACAAAGCGGTGCCAGAGGGCAGGGTTGTATGGAATGCGTTTGCAGAT‑GTAAAAAATCCCCCCGAGCGAAAAACCCACGCCGCCTCCCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTaa < 2:305064/148‑1 (MQ=255) aaaatgCACACACTACCGCTGATCACGAAGCCGTGACAGATGGCATGGATATCTGGGATGCGTTTGCAGAC‑GTAGAAAATCACCGCGAGGGAATAAACGACGCCGCCTACCACCGGGAAAATAACGCTGCCAGCCTCAAGATTAACTg > 1:459716/6‑148 (MQ=255) ggccacacaCTACCGCCGGGCACAAGGCCGTCCGAGAGGCCGTGTTTTTATGAATTGCGTTGGCAGCC‑GGAGAAAACCCCCCGGGGCAAAAAACCCACCCCCCCTCCCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCAttt < 2:593897/148‑1 (MQ=255) cccgcccgAGCAGGAGGCGGTGCGAGAGGCCAGGTTTTTGTGGAATGGTTTGGCGGCC‑GTAAAAAACCACCCCGGCCAAATAACCCACGCCGCCCACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTac < 2:979534/146‑1 (MQ=255) aCCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGAC‑GTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCAAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTa > 2:742456/1‑149 (MQ=255) ccgccgAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGAC‑GTAGAAAATCCCCCCGAGCGAATAACCCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAAtt < 2:563026/147‑1 (MQ=255) cgAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGAC‑GTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTaccacc > 2:56703/1‑149 (MQ=255) gAGCAGGAGGCCGGGCAAGAGGGCGTGTTGGTATGAAAGGCGTTGGCACAC‑TTAAAAAACCCCCCCGAGCAAAAAACCCCCGCCCCCTCCCGCCAGTAAGTTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTAccacca < 1:212482/149‑1 (MQ=255) cACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCTTTTGCAGAC‑GTAAAAAATCCCCCCGAGCAAATAACCCCCGCCCCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGa < 2:127491/150‑1 (MQ=255) cACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGAC‑GTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGAc > 1:84572/1‑151 (MQ=255) aCGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGAC‑GTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGAc > 2:908664/1‑150 (MQ=255) gAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGAC‑GTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGAcagc > 2:48150/1‑151 (MQ=255) aggCCGTGCAAGAGGGCAGGGTTGTAGGAAGGGCTTTGGCAGAG‑GTAAAAAATCCCCCCGAGCAAAAAACCCCCGCCGCCTCCCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTaccac < 1:990477/138‑1 (MQ=255) agcccggcccAGAGGGCATGGTTTTAGGGAATGCTTTTGCAGAG‑GTAAAAAACCCCCCCGAGCGAAAAACCCCCGCCCCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGAcagc < 2:811234/142‑1 (MQ=255) aaGCCGGGCAAGATGGCTTGGTTGTAGGGAATGCTTTTGCAGAC‑GTAAAAAATCCCCCCGAGCAAATAACCCCCGCCCCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGAcagc < 2:456981/150‑1 (MQ=255) cggTGCCAGAGGGCTTGTTTGTATGGAATGCGTTTGCAGAC‑GTAAAAAATCCCCCCGAGCAAATAACCCCCGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGAcagcca < 2:1153510/147‑1 (MQ=255) tGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGAC‑GTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGccc > 2:418948/1‑150 (MQ=255) ccAGAGGGCATGTTTTTAGGGAATGCTTTTGCAGAC‑TTAAAAAACCCCCCCGAGCAAAAAACCCCCCCCCCCTCCCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCAt < 1:929414/150‑1 (MQ=255) tGGCTTGTTTTTTTGAAATGCGTTTGCAGAG‑TTAAAAAACCCCCCCGAGCGAAAAACCCCCCCCCCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGcc < 2:538098/149‑1 (MQ=255) ggCAGGTTTGTATGGAAGGTTTTTGGAGAC‑GTAAAAACCCCCCCGGGGCGAATAACCCCCCCCGCCTCCCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCg < 2:220300/146‑1 (MQ=255) gccATGTTTGTAGGAATTGTTTTTGCAGCC‑GAAAAAAACCCCCCCGGGCAAAAAACCCCCCCCCCCTCCCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGcc < 1:604190/146‑1 (MQ=255) gccATGGTTGTATGGATTGCTTTGGCAGCC‑GTAAAAAACCCCCCCGAGCAAAAAACCCCCCCCCCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATc < 2:220745/143‑1 (MQ=255) gcaaTGTTTGTATGAAATG‑GTTTGCTGGCGGTAGAAAACCCCCCGGGGCAAAAAACCCCCGCCGCCTTCCGCCAGTAAGGTAACGTTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGa < 2:896066/148‑1 (MQ=255) gtttGTAGGGAAGGCTTTGGCAGAC‑GAAAAAAACCCCCCCGAGCGAAAAACCCCCGCCCCCTCCCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGt < 2:1038330/149‑1 (MQ=255) tGTATGGAATGCGTTTGCAGAC‑GTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCAc > 2:379448/1‑151 (MQ=255) aTGAAATGCGTTTGCAGAC‑GTAAAAAATCCCCCCGAGCGAAAAACCCACGCCCCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCa < 2:50402/150‑1 (MQ=255) ggAATGCGTTTGCAGAC‑GTAAAAAACCCCCCCGAGCAAAAAACCCCCGCCCCCTCCCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCaga < 2:272543/150‑1 (MQ=255) gaaggccttttGCGGCC‑GAAAAAAACCCCCCCGGGCAAAAAACCCCCCCCCCCTCCCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCagag < 1:120614/143‑1 (MQ=255) aTGCGTTTGCAGAC‑GTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGAt > 2:943541/1‑150 (MQ=255) gTTTGCAGAC‑GTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAAt > 1:817837/1‑151 (MQ=255) gTTTGCAGAC‑GTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAAt > 1:531710/1‑151 (MQ=255) tttGCAGCC‑TAAAAAAACCCCCCCGAGCAAAAAACCCCCCCCCCCTCCCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATa < 1:174409/146‑1 (MQ=255) ttggCAGAC‑GAAAAAAACCCCCCCGGGCAAAAAACCCCCCCCCCCTCCCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATaa < 1:531947/144‑1 (MQ=255) tttggggcggTAAAAAATCCCCCCGAGCAAAAAAACCGCGCCCCCTCCCGCCATTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATaaa < 2:316134/139‑1 (MQ=255) aC‑GTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATc > 2:257710/1‑151 (MQ=255) gaaaaaaaCCCCCCCGAGCAAAAAACCCCCCCCCCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATc < 2:520207/145‑1 (MQ=255) aaaaTCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTgcg > 1:219930/1‑151 (MQ=255) aaaTCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTgcg > 1:386178/1‑150 (MQ=255) | GCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGAC‑GTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCG > NC_000913/3042456‑3042748 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |