Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,743,6690GA42.9% 60.2 / 28.7 56E313K (GAA→AAA) yiaK2,3‑diketo‑L‑gulonate reductase, NADH‑dependent
Reads supporting (aligned to +/- strand):  ref base G (28/4);  new base A (0/24);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 9.40e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

TATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCCATGAATTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCG  >  NC_000913/3743521‑3743805
                                                                                                                                                        |                                                                                                                                        
tATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCCATGAATTTACTACCCTGCTGGCCGa                                                                                                                                         >  1:718468/1‑150 (MQ=255)
 aTTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCCATGAATTTACTACCCTGCTGGCCGaa                                                                                                                                        >  1:258684/1‑150 (MQ=255)
    gCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCCATGAATTTACTACCCTGCTGGCCGAAAAc                                                                                                                                     <  2:881445/150‑1 (MQ=255)
           aaGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCCATGAATTTACTACCCTGCTGGCCGAAAACCGCCGTa                                                                                                                              >  2:590168/1‑150 (MQ=255)
           aaGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCCATGAATTTACTACCCTGCTGGCCGAAAACCGCCGTa                                                                                                                              >  2:530858/1‑150 (MQ=255)
                   aaGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCCATGAATTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATc                                                                                                                      >  2:573825/1‑150 (MQ=255)
                      cTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCCATGAATTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACt                                                                                                                   >  1:11269/1‑150 (MQ=255)
                      cTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCCATGAATTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTg                                                                                                                  >  1:432767/1‑151 (MQ=255)
                         accgcggccccccccCGCGATGCCAAGCGGCACCGCACAATGGTTTCCGTTCCTAGGGCGGGGCGGGTTGAAAAAAACCGGGCAATTCGCTCCCCGGGCCATAATTTTACTCCCCTGGGGGCGAAAAACCGCCGTAACGGCATCACTGTtg                                                                                                               <  2:352267/142‑1 (MQ=255)
                          tCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCCATGAATTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTtg                                                                                                               >  1:672992/1‑150 (MQ=255)
                           cgccGGCCCCCCCCGCGATGCAACGTTGAAACGAATCATGGTTTACGTTCCGAGGCCCGAGGGTGCTGACAAAAACCAGCCCATCCCCTCCCCCGCCCATGATTTTTCTCCCCTCCGGGCAGAAATCCGCCGTAACGGCATCACTg                                                                                                                  <  1:1151351/143‑1 (MQ=255)
                             aCGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCCATGAATTTACTACCCTGCTGGCTGAAAACCGCCGTAACGGCATCACTGTtgatg                                                                                                            >  1:571432/1‑150 (MQ=255)
                              cGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCCATGAATTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTtgatg                                                                                                            >  1:453490/1‑149 (MQ=255)
                               ggcccccccCGCGATGCAAAGCGGAAACCAATATGGGTTTCCGTTACTGGTGCGGGGGGGGCTGAAAAAAATCGGCCCATCCGCTTCCCCGCCAATAATTTTCCTCCCCTGCGGGCCAAAAACCGCCGTAACGGCATCACTGTTGATGAc                                                                                                          <  2:642289/147‑1 (MQ=255)
                                 tCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCCATGAATTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGc                                                                                                       >  2:1014553/1‑151 (MQ=255)
                                    cccccGCGATGCCAAGCTGCACCGCATAAGGGTTTACGTTACTAGGGCCGGGCGTGCTGACAAAAACCAGCCCATCCGCTTCCCCGGCCATAATTTTACTCCCCTGCGGGCCAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCgtg                                                                                                    <  2:702640/149‑1 (MQ=255)
                                      cccgcggtcaaaaGCTGCAACGAATCGTGTTTTACGTTACTGGGCCGGGGCGTGCTGACAAAAATAAGGCCTTCCTCTCCCCCGGCCATAATTTTACTCCCCTGCGGGCCAAAACCCGCCGTAACGGCATCACTGTTGATGACAGCgtgt                                                                                                   <  1:106549/139‑1 (MQ=255)
                                        cgcgATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCCATGAATTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTggg                                                                                                >  2:635300/1‑151 (MQ=255)
                                              gCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCCATGAATTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCaaa                                                                                           >  1:1015234/1‑150 (MQ=255)
                                                 aaGCTGCAACCAATCAGGGTTTACTTTCCTGGTGCCGGGCGTGAGGAAAAAAACCAGCCCTTTCGCTTCCCGGCCAAAAATTTTACTCCCCTGCGGCCAAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTgg                                                                                                 <  1:734359/141‑1 (MQ=255)
                                                     taaaacacaaaatGGATTGCTTTACTAGCGCGGGGCGTGTTGACAAAAAACGGGCCCTTCGTTTCCCGGCCAATAATTTTCCCCCCCGGCGGCCCAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAg                                                                                     <  1:1004806/138‑1 (MQ=255)
                                                        accGCATCATGTTTTCCTTTACTGGTGCGGGGCGTGCTGAAAAAAATCAGCCCTTCCTCTTCCCCGGCCATAATTTTCCTCCCCTGCGGGCCAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAgg                                                                                    <  2:669831/145‑1 (MQ=255)
                                                                atGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCCATGAATTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGCCAGCGTGTGGGCCAAAGTCCAGGCGTTATgagg                                                                          >  1:1008267/1‑149 (MQ=255)
                                                                  gttttccGTTACTGGGGCGGGGGGTGCTGAAAAAAATCAGGCCTTTCGCTTCCCCGGCCATAATTTTCCTCCCCTGCGGGCCAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATgag                                                                           <  2:861341/140‑1 (MQ=255)
                                                                  ggATTACTTTACTAGGGCCGGGCGTGCTGAAAAAAACCGGCCCTTTCGTTTCCCCGCCAATGAATTTACTCCCCTGCGGGCCAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATgag                                                                           <  2:1135027/146‑1 (MQ=255)
                                                                   gATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCCATGAATTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGAt                                                                      >  1:109984/1‑150 (MQ=255)
                                                                   gATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCCATGAATTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGAt                                                                      >  1:314861/1‑150 (MQ=255)
                                                                     ttcctttACTAGGGCCGAGCGTGCGGAAAAAAACCAGCCCATTCCCTTACCCGCCCATAAATTTACTCCCCTGCGGCCCAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGAt                                                                      <  2:807822/143‑1 (MQ=255)
                                                                     ttagtttACTGGGGCGGAGCGTGCTGACAAAAACCAGCCCATTCGTTTCCCCGGCCATAATTTTACTCCCCTGCTGGCCAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATgagga                                                                         <  2:1036799/140‑1 (MQ=255)
                                                                     ttACGTTACTGGGCCCGGGCTTGCGGAAGAAAAGCAGCCCATGCGCTCCCCCGCCCATGATTTTCCCCCCCGCCGGGCCAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATa                                                                     <  2:316978/149‑1 (MQ=255)
                                                                        cGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCCATGAATTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTc                                                                 >  1:82881/1‑150 (MQ=255)
                                                                          taagtggtcccGGGCGTGCGGAAAAAATCCAGCCCTTCCGCTCCCCCGGCCATAAATTTCCCCCCCGGCGGGCCAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGt                                                                  <  2:881950/138‑1 (MQ=255)
                                                                            aCTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCCATGAATTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGAt                                                            >  1:1126774/1‑151 (MQ=255)
                                                                              tAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCCATGAATTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGAttt                                                          >  1:269044/1‑151 (MQ=255)
                                                                                  gCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCCATGAATTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTgg                                                      >  1:798192/1‑151 (MQ=255)
                                                                                  ccggAGCGTGTAGAAAAAAACCAGGCCATCCGTTCCCCCGGCCATGAATTTACTCCCCTGTTGCCAAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTg                                                       <  2:258943/147‑1 (MQ=255)
                                                                                   ccGGGCGTGCTGAAAAAAACCAGGCCATCCGCTCCCCCGGCCATAATTTTCCTCCCCTGCGGGCGAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGa                                                     <  2:388286/151‑1 (MQ=255)
                                                                                     gggCGTGCTGACAAAAACCAGCCCTTTCGCTTCCCCGGCAATAATTTTACTCCCCGGCTGGCAAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGAc                                                    <  2:1127464/148‑1 (MQ=255)
                                                                                         gTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCCATGAATTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATc                                               >  1:325557/1‑151 (MQ=255)
                                                                                           gctaaaaaaaaTCAGCCATTTCTCTTCCCCGGCCGTAATTTTCCTCCCCGGTTGGCAAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGAc                                                    <  2:1114833/137‑1 (MQ=255)
                                                                                                  aaaaCCAGGCCTTTCGCTTCCCCGGCCATGATTTTACTCCCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGcc                                       <  2:808322/150‑1 (MQ=255)
                                                                                                   aaaTCACGCCATCCGCTTCCCCGACTATGATTTAACTCCCCTGGGGGCAAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGccc                                      <  1:825071/150‑1 (MQ=255)
                                                                                                           ccATTCGCTTACCCGGCCATGAATTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGc                             >  1:1083686/1‑151 (MQ=255)
                                                                                                              tccccTTCCCCGCCCATGATTTTCCTCCCCTGCGGCCAAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATcc                                 <  2:1126833/140‑1 (MQ=255)
                                                                                                                cGCTCACCCGGCCATAAATTTCCTCCCCTGCTGGCCAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCg                           <  1:537232/148‑1 (MQ=255)
                                                                                                                 ccctccccGGCCATTAAATTTCCTCCCCGGGGGGCCAAAACCCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATcc                                 <  1:739863/137‑1 (MQ=255)
                                                                                                                   ttccccGGCAATAATTTTCCTCCCCTGCGGGCCAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGcc                            <  2:982025/141‑1 (MQ=255)
                                                                                                                      cccGCCCATGAATTTCCCCCCCTGCTGCCGGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGc                      <  2:60625/147‑1 (MQ=255)
                                                                                                                          gCCATGTATTTACCCCCCTTGCGGGCAAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCccgcc                 <  1:627940/149‑1 (MQ=255)
                                                                                                                              aTGAATTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGGGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCAtt           >  2:1052794/1‑151 (MQ=255)
                                                                                                                                 gAATTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTg          >  1:257221/1‑150 (MQ=255)
                                                                                                                                   attttCCTCCCCTGCGGGCCAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCgccgcc              <  2:1079605/143‑1 (MQ=255)
                                                                                                                                   aTTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCACAATCCAGGCGTTCTGAGGAGATCAGTCATGATTTTTGGACATATCGCGCAGACCAATCCGTGCCGTTTGCCCGCCGCCATTg          >  1:189015/1‑148 (MQ=255)
                                                                                                                                   aTTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGaa        >  2:145285/1‑150 (MQ=255)
                                                                                                                                      ttACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGaaa       >  2:251430/1‑149 (MQ=255)
                                                                                                                                         ctacCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGgcg  >  1:1148024/1‑151 (MQ=255)
                                                                                                                                                        |                                                                                                                                        
TATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCCATGAATTTACTACCCTGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCG  >  NC_000913/3743521‑3743805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: