Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 366,539 | 0 | C | T | 47.2% | 50.4 / 26.1 | 53 | V324V (GTG→GTA) | lacI | lactose‑inducible lac operon transcriptional repressor |
Reads supporting (aligned to +/- strand): ref base C (13/15); new base T (25/0); total (38/15) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.51e-06 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGG‑TTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATT‑G‑CCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTAT > NC_000913/366393‑366687 | aaTGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGG‑TTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCa < 1:896561/151‑1 (MQ=255) aTGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGG‑TTTTCGGA‑TTTGGCGCCCGGGGGGGTTTTTTTTTTAcc > 1:296935/1‑149 (MQ=255) aCTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGG‑TTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCaa < 2:341218/151‑1 (MQ=255) tCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGG‑TTTTTGGA‑TTTGGGGCCCGGGGGGGTTTTCTTTTTACCAGTGAGA‑CGGGCaa > 2:859052/1‑149 (MQ=255) ttAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGG‑TTTGGGTATTTGGGGGCCGGGGGGGTTTTT‑TTTTTACCCGTGAGA‑CGGGCAACa > 2:104448/1‑146 (MQ=255) ttAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGG‑TTTGCGTA‑TTTGGCGCCCGGGGGGGTTTTTTTTTTTCCAGTGAGA‑CGGGCAAc > 2:759837/1‑145 (MQ=255) ttgcgttgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGG‑TTTTCGTA‑TTTGGGGCCAGGGGGGGTTTTTTTTTTACCCGTGAGA‑CGGGGAACAGCTGAtt > 2:353014/1‑149 (MQ=255) gttgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGG‑TTTTGGGA‑TTGGGGGGCCGGGGGGGTTTTTTTTTTTCCAGGGAGA‑AGGGGGACAGCTGATT‑G‑tcc > 1:748805/1‑149 (MQ=255) ttgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGG‑TTTGCGTG‑TTTGGGGCCCGGGGGGGTTTTTTTTTTACCAGGGAGA‑CGGGGAACAACTGATT‑G‑cccct > 1:567202/1‑148 (MQ=255) cACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGG‑TTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATT‑G‑CCCTTCACCGc < 2:885320/149‑1 (MQ=255) aCTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGG‑GTTGGGGT‑TTTGGCGCCCGGGGGGGTTTTCTTTTTCCCCGTGGGA‑CGGGCAACAGCTGATT‑G‑CCCCTCACcccc > 1:195724/1‑149 (MQ=255) tGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGG‑TTTTCGTG‑TTGGGGGGCAGGGGGGGTTTTTTTTTTACCAGGGAGG‑AGGGGAACAGCTGGTT‑G‑CCCTTCACcccc > 1:764185/1‑147 (MQ=255) gCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGG‑CTTTCGTA‑TTGGGGGCCCGGGGGGGTTTTTTTTTCACCCAGGAGA‑CGGGGCACAGCCGGTTGG‑CCCTTCccccccgg > 1:486255/1‑147 (MQ=255) ccGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGG‑TTTTGGTG‑TTGGGGGGCAGGGGGGGTTTTTTTTTTCCCCGGGAGA‑CGGGCAAAACCCGATT‑G‑CCCCTCACCCCCCGGccc > 2:781833/1‑150 (MQ=255) ccGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGG‑TTTTCGTA‑TTTGGGGCCCGGGGGGGTTTTTTTTTTTCCAGTGAGA‑CGGGCAACAGCTGATT‑T‑CCCTTCACCCCCCTGccc > 2:224325/1‑150 (MQ=255) ccGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGG‑TTTGCGTA‑TTTGGGGCCCGGGGGGGTTTTTTTTTTACCAGTGAGA‑CGGGCAAAAGCTGATT‑G‑CCCTTCACCCCCTGGccc > 2:221220/1‑150 (MQ=255) ccGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGG‑TTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATT‑G‑CCCTTCACCGCCTGGCCCt < 2:106325/151‑1 (MQ=255) gCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGG‑TTTTGGGA‑TTGGGCGCCCGGGGGGGTTTTTTTTTTACCCGTGAGA‑CGGGGGACAGCTTGTT‑G‑CCCCTCCCCCCCCGGccc > 1:915757/1‑148 (MQ=255) cGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGG‑TTTTCGGA‑TTGGGGGCCCGGGGGGGTTTTTTTTTTCCCAGTGAGG‑CGGGCAACAACTGATT‑G‑CCCTTCACCGCCCGGCCCCGagagaggt > 2:878026/1‑146 (MQ=255) cGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGCCCAACGCGCGGGGAGAGGCGG‑TTTTCGTA‑TTTGGGGCCCGGGGGGGTTTTTTTTTCACCAGTGAGA‑CGGGGCACAGCTGATT‑G‑CCCTTCACCCCCCGGCCCTGGGAGAGGTg > 2:713345/1‑149 (MQ=255) gggAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGG‑TTTTCGTA‑TTTGGGGCCCGGGGGGGTTTTTTTTTTACCAGTGAGA‑AGGGGAACAACTGATT‑G‑CCCTTCACCCCCCGGCCCCGagagaggt > 2:244512/1‑145 (MQ=255) aaaCCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGG‑TTTTCGTA‑TTTGGCGCCCGGGGGGTTTTTTTTTTTACCAGTGAGA‑CGGGCAAAAGCTGATT‑T‑CCCTTCACCCCCTGGCCCTGAGAGAGGTgcagca > 2:1116175/1‑150 (MQ=255) aaCCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGG‑TTTTCGTA‑TTTGGGGCCAGGGGGGGTTTTTTTTTTACCCGTGAGA‑CGGGCAAAAGCGGATT‑G‑CCCTTCCCCCCCCGGCCCTGAGAGAGGTGCAGCaa > 2:123952/1‑150 (MQ=255) aCCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGG‑TTTTCGTA‑TTTGGGGCCAGGGGGGGTTTTCTTTTTACCCGTGAGA‑CGGGGAACAGCTGATT‑G‑CCCTTCACCCCCTGGCCCTGAGAGAGGTGCAGCaa > 2:258877/1‑149 (MQ=255) tGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGATTTTCTAT‑TTTGGCGCCGGGGGGGTTTTT‑TTTTTACCCGTGAGA‑CGTGCACCAGCACCTT‑GACCCTCCACC‑CCTGTTCCTCagagaggtttatcaatccg > 1:209794/1‑137 (MQ=255) tGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGG‑TTTTCGTA‑TTTGGGGCCCGGGGGGGTTTTTTTTTTCCCAGTGAGA‑AGGGCAACAGCTGATT‑G‑GCCTTCACCGCCCGGCCCTGAGAGAGGTGCAGCAAGCggg > 1:1025718/1‑150 (MQ=255) gTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGG‑TTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATT‑G‑CCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTc < 1:218461/151‑1 (MQ=255) cGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGCTTTCGGCT‑TTGGGGGCCGGGGGGGTTTTT‑TTTTTACCCCTGGGA‑CGGGGCACAGCCTAAT‑G‑TCCTTCACCCCCCCGCCCCGTGAGAGtgggcgccagccggcc > 1:280624/1‑135 (MQ=255) cGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGG‑TTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATT‑G‑CCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCa < 1:136403/151‑1 (MQ=255) cAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGG‑TTTTCGGA‑TTTGGCGCCCGGGGGGGTTTTTTTTTTACCCCTGAGA‑CGGGGAACAGCTGATT‑T‑CCCCTCCCCCCCCGGCCCTGAGAGAGGTGCAGCCAACGGTCCAc > 1:563343/1‑147 (MQ=255) cATTAATGAATCGGCCAACGCGCGGGGAGAGGCGG‑TTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATT‑G‑CCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGc < 1:598537/151‑1 (MQ=255) aTTAATGAATCGGCCAACGCGCGGGGAGAGGCGG‑TTTTGGGG‑TTTGGGGCCCGGGGGGGTTTTTTTTTTTCCAGTGGGA‑CAGGCAAAAACCGATT‑G‑CCCTTCCCCGCCAGGCCCTCGGAGAGGTGCAGCAAGAGGGCCACGCCGGgtt > 2:760125/1‑148 (MQ=255) aTTAATGAATCGGCCAACGCGCGGGGAGAGGCGG‑TTTTCGTA‑TTTGGGGCCCGGGGGGGTTTTTTTTTTACCAGTGAGA‑CGGGGAAAAGCTGATT‑T‑GCCTTCACCGCCTGGGCCTGAGAGAGTTGCAGCAAACGGGCCACGCCGGGTTTcc > 2:740777/1‑151 (MQ=255) tAATGAATCGGCCAACGCGCGGGGAGAGGCGG‑TTTTCGTA‑TTTGGCGCCCGGGGGGGTTTTTTTTTTCCCCGTGAGA‑CGGGCAACAACCGATT‑G‑CCCCTTACCGCCCGGCCCTGAGAGAGGTGGAGCAAACGGGCCACGCCGGGTTTcccc > 2:1015672/1‑151 (MQ=255) aTGAATCGGCCAACGCGCGGGGAGAGGCGG‑TTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATT‑G‑CCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCcag < 2:395138/151‑1 (MQ=255) gAATCGGCCAACGCGCGGGGAGAGGCGG‑TTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATT‑G‑CCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCcagca < 1:416480/151‑1 (MQ=255) aaTCGGCCAACGCGCGGGGAGAGGCGG‑TTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATT‑G‑CCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCcagcag < 1:1152805/151‑1 (MQ=255) ggCCAACGCGCGGGGAGAGGCGG‑TTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATT‑G‑CCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGa < 2:995191/151‑1 (MQ=255) ggCCAACGCGCGGGGAGAGGCGG‑TTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATT‑G‑CCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGa < 1:1119601/151‑1 (MQ=255) ccAACGCGCGGGGAGAGGCGG‑TTTGGCTC‑ATAGGGGCCCGGGGGGTTTTTCTTTTCATCAGTGAGACCGGGCAA‑AGCGGACT‑G‑GCCTTTTCCGCCTTGCCCCGCGGGAGGTGCAGCAAGCCGGTCACCCTGGGGTGCCCCAGCAGGcaca > 2:611104/1‑147 (MQ=255) gg‑TTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATT‑G‑CCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGtt > 2:560599/1‑151 (MQ=255) tttGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATT‑G‑CCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTa > 1:1105291/1‑150 (MQ=255) a‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATT‑G‑CCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCggg > 2:523826/1‑151 (MQ=255) cgcCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATT‑G‑CCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCggg > 2:577047/1‑145 (MQ=255) cgcCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATT‑G‑CCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGAtata > 1:155657/1‑150 (MQ=255) cAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATT‑G‑CCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATaaaa > 1:803836/1‑148 (MQ=255) aGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATT‑G‑CCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATaaaa > 1:524799/1‑147 (MQ=255) ggtggtTTTTCTTTTCACCATTGAGA‑CGGGCAACAGCTGATT‑G‑CCCTGCACCGCTTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATg < 2:751533/149‑1 (MQ=255) ggtggtTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATT‑G‑CCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAg < 1:581916/151‑1 (MQ=255) gtggtTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATT‑G‑CCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGGTATAACATGa > 1:333607/1‑149 (MQ=255) gtggtTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATT‑G‑CCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATg > 1:1117874/1‑148 (MQ=255) ggtTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATT‑G‑CCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCt < 1:759800/150‑1 (MQ=255) gtTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATT‑G‑CCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGt > 2:307292/1‑151 (MQ=255) cACCAGTGAGA‑CGGGCAACAGCTGATT‑G‑CCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGCTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATCACATTAGCTGTCTTCGGTAt > 1:946323/1‑149 (MQ=255) | AATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGG‑TTTGCGTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGA‑CGGGCAACAGCTGATT‑G‑CCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTAT > NC_000913/366393‑366687 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |