| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 665,554 | C→T | E254K (GAA→AAA) | mrdB ← | cell wall shape‑determining protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 665,554 | 0 | C | T | 98.2% | 207.8 / ‑3.4 | 56 | E254K (GAA→AAA) | mrdB | cell wall shape‑determining protein |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (24/31); total (24/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01 | |||||||||||
CCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTCAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGCAATTTTAGACTGAATAATGTGATAGCCCGCGCCGAGTGGGTCTGATTCCGGGTCCAGGAGCATCATTACGCGCTGGCGCTGGT > NC_000913/665406‑665697 | ccAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTaa < 1:846080/151‑1 (MQ=255) gtggtTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCtgtg > 2:1132774/1‑151 (MQ=255) ggtTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGAc < 1:513260/151‑1 (MQ=255) cgcTCTGGCGCGTATCCACAGCCCGTGCATGCTCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGt > 1:916471/1‑150 (MQ=255) gcTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGc > 2:702365/1‑151 (MQ=255) ctctGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTg < 2:793296/149‑1 (MQ=255) ggcggcTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTg < 1:222264/150‑1 (MQ=255) gcTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGcagcc > 1:294855/1‑151 (MQ=255) tATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGcagcc > 1:848939/1‑149 (MQ=255) acaGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCttt < 2:559502/151‑1 (MQ=255) caGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCttt < 1:1109574/150‑1 (MQ=255) ccGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCcgc > 1:171412/1‑151 (MQ=255) ccGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCcgc < 1:655873/151‑1 (MQ=255) gcATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGGAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTa > 1:174457/1‑150 (MQ=255) gcATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTa > 2:440310/1‑150 (MQ=255) aTGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCGCTAACCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATc < 2:190790/151‑1 (MQ=255) aTGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAAt > 2:132341/1‑150 (MQ=255) aTGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATc > 1:1142461/1‑151 (MQ=255) tGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATcc > 1:614896/1‑151 (MQ=255) aTCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATccgc < 2:580848/151‑1 (MQ=255) agcagAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCgg < 1:25199/151‑1 (MQ=255) aaTGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCa < 1:634265/151‑1 (MQ=255) gTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATa < 1:166477/151‑1 (MQ=255) tAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAAt > 1:416706/1‑149 (MQ=255) tAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATa < 2:467781/150‑1 (MQ=255) agagagCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGc > 1:973421/1‑151 (MQ=255) agagagCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGc < 2:969639/151‑1 (MQ=255) gagcgagcAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGCAAt < 2:238541/151‑1 (MQ=255) gcAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGCAATTTTAg < 2:358963/150‑1 (MQ=255) agaatcagaatGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGCAATTTTAGACt < 2:139273/151‑1 (MQ=255) atcagaatGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGCAATTTTAAACTGaa < 2:299128/151‑1 (MQ=255) gaatGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTCAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGCAATTTTAGACTGaataat < 2:531136/151‑1 (MQ=255) cccACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGCAATTTTAGACTGAATAATGTGAt > 1:257428/1‑151 (MQ=255) ccACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGCAATTTTAGACTGAATAATGTGATa < 2:1010095/151‑1 (MQ=255) cACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGCAATTTTAGACTGAATAATGTGAt > 2:496575/1‑149 (MQ=255) cTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGCAATTTTAGACTGAATAATGTGATAg < 1:784857/149‑1 (MQ=255) aaTCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGCAATTTTAGACTGAATAATGTGATAGccc < 2:1141158/150‑1 (MQ=255) aTCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGCAATTTTAGACTGAATAATGTGATAGCCcgc > 2:776581/1‑151 (MQ=255) ccAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGCAATTTTAGACTGAATAATGTGATAGCCCGCGcc < 2:130725/151‑1 (MQ=255) ccAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGCAATTTTAGACTGAATAATGTGATAGCCCGCGcc < 2:861937/151‑1 (MQ=255) ctctTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGCAATTTTAGACTGAATAATGTGATAGCCCGCGCCGAGt > 2:766799/1‑151 (MQ=255) tctTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGCAATTTTAGACTGAATAATGTGATAGCCCGCGCCGAGTg > 1:103318/1‑151 (MQ=255) cGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGCAATTTTAGACTGAATAATGTGATAGCCCGCGCCGAGTGGGTCt < 1:1050842/151‑1 (MQ=255) cGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGCAATTTTAGACTGAATAATGTGATAGCCCGCGCCGAGTGGGTCt < 1:845007/151‑1 (MQ=255) aGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGCAATTTTAGACTGAATAATGTGATAGCCCGCGCCGAGTGGGTCTGAtt > 1:927074/1‑151 (MQ=255) tACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGCAATTTTAGACTGAATAATGTGATAGCCCGCGCCGAGTGGGTCTGATTcc > 2:565962/1‑151 (MQ=255) aCCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGCAATTTTAGACTGAATAATGTGATAGCCCGCGCCGAGTGGGTCTGATTCCg < 2:803067/151‑1 (MQ=255) aaGATAAAGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGCAATTTTAGACTGAATAATGTGATAGCCCGCGCCGAGTGGGTCTGATTCCGGGTcc > 1:1062223/1‑150 (MQ=255) aaaGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGCAATTTTAGACTGAATAATGTGATAGCCCGCGCCGAGTGGGTCTGATTCCGGGTCCAGGAGc < 1:991017/151‑1 (MQ=255) aaaGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGCAATTTTAGACTGAATAATGTGATAGCCCGCGCCGAGTGGGTCTGATTCCGGGTCCAGGAGc < 1:123020/151‑1 (MQ=255) aaaGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGCAATTTTAGACTGAATAATGTGATAGCCCGCGCCGAGTGGGTCTGATTCCGGGTCCAGGAGc < 1:1086310/151‑1 (MQ=255) aaGTCAGTATGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGCAATTTTAGACTGAATAATGTGATAGCCCGCGCCGAGTGGGTCTGATTCCGGGTCCAGGAGc > 2:767238/1‑150 (MQ=255) aTGGCGTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGCAATTTTAGACTGAATAATGTGATAGCCCGCGCCGAGTGGGTCTGATTCCGGGTCCAGGAGCATCATTAcg < 2:1090880/151‑1 (MQ=255) gTTCGGGGAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGCAATTTTAGACTGAATAATGTGATAGCCCGCGCCGAGTGGGTCTGATTCCGGGTCCAGGAGCATCATTACgcgctg < 2:257966/151‑1 (MQ=255) gggAGAAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGCAATTTTAGACTGAATAATGTGATAGCCCGCGCCGAGTGGGTCTGATTCCGGGTCCAGGAGCATCATTACgcgctggcgct > 1:126400/1‑151 (MQ=255) gaAATTTAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGCAATTTTAGACTGAATAATGTGATAGCCCGCGCCGAGTGGGTCTGATTCCGGGTCCAGGAGCATCATTACGCGCTGGCGCTGGt > 1:515037/1‑150 (MQ=255) | CCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGGAGAAATTCAAGCTGTGACTGAGTGCCGTGCAGCCAGCCTTTGCCGCGTAATCCGCCGGAGCCAATAGCAATTTTAGACTGAATAATGTGATAGCCCGCGCCGAGTGGGTCTGATTCCGGGTCCAGGAGCATCATTACGCGCTGGCGCTGGT > NC_000913/665406‑665697 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |