Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 847,444 | 0 | G | T | 45.5% | 73.8 / 34.0 | 55 | I187I (ATC→ATA) | glnH | glutamine transporter subunit |
Reads supporting (aligned to +/- strand): ref base G (5/25); new base T (25/0); total (30/25) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 8.87e-11 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTG‑CGC‑GGTTGGTGCCCAGTTCCATATAGGCGTTATCGATGTTCGGGAACTGACGCAGATCTTTAGTTTTGATGTTTGCTTTCGCGTAATCAACGGAGCCAGTACC > NC_000913/847295‑847590 | tCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTtg < 1:556962/150‑1 (MQ=255) gTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTttat > 2:1042484/1‑147 (MQ=255) gTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTGATGaa < 2:95192/150‑1 (MQ=255) gTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTGATGAAg < 1:837955/151‑1 (MQ=255) gTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTGATGAAGTACAGAATg < 2:591979/151‑1 (MQ=255) ttCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTTGTGAAGTAAAGa > 1:75570/1‑147 (MQ=255) tctcGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTTATGTAGGACAGaa > 1:1004489/1‑147 (MQ=255) cgcAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTGATGAAGTACAGAATGTTTGGCg < 1:23337/151‑1 (MQ=255) ggTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTGATGAAGTACAGAATGTTTGGCGTATCg < 1:708405/151‑1 (MQ=255) tttCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTTGTTAAGGACAGAATGTTTGGGGTGTCGttg > 1:404/1‑149 (MQ=255) tttCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTGATGAAGTACAGAATGTTTGGCGTATCGTg < 2:985641/150‑1 (MQ=255) ttCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTTTTGAAGTAAAGAAAGGTTGGCGGAt > 1:320695/1‑145 (MQ=255) tCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTTGTTGAGGACAGAAAGTTTgggggattgt > 2:697318/1‑139 (MQ=255) cgcgCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACg < 1:555442/151‑1 (MQ=255) gcgcCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTTTTGAAGGATAAAATGTTTTGGGTTTTGTGccgga > 1:19809/1‑144 (MQ=255) gcCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCg < 1:884967/151‑1 (MQ=255) gTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCt < 2:531387/151‑1 (MQ=255) ttGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTG‑cgc‑g < 2:337088/149‑1 (MQ=255) ttGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTG‑cgc‑g < 2:36917/149‑1 (MQ=255) cTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTTATGAAGGACAGAATGTTTGGGGGATCGTGCAGAACGGCGTCTG‑CGC‑GGGTGGTGgcc > 1:339715/1‑148 (MQ=255) cTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTGTTGAAGTAAAGAAAGTTTTGCGTATCGTGCCGAAAGGCGTCTG‑CGC‑GGGTGGTGccc > 1:690885/1‑148 (MQ=255) cgCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTG‑CGC‑GGTTGGTGCCCAGTTCCat < 1:144911/151‑1 (MQ=255) gCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCGCCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTG‑CGC‑GGTTGGTGCCCAGTTCCata < 1:370340/151‑1 (MQ=255) tACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTTTTTGAGGACAGAATGTTTTGGGGATTTGGCCGAACCGCGGCTT‑CGC‑GGGTTGTGGCCCGtttc > 2:303930/1‑144 (MQ=255) tACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTTTTGGAGTAAAGAATGTTTTGGGGGTCGTGCAGAACGGCGGGTG‑CGC‑GGGTGGTGGCCAggt > 1:1077691/1‑142 (MQ=255) tCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTG‑CGC‑GGTTGGTGCCCAGTTCCATATAGGCGt < 1:361289/150‑1 (MQ=255) gggAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTTTTGAAGGACAGAAAGGTTTGGGTATTGGGCAGAAAGGGGGCTG‑CGC‑GGGTGGTGGCCCGTTTCATATAAGCGtttt > 1:1080916/1‑149 (MQ=255) gggAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTTATTTAGTACAGAAAGTTTTGGGGATCGTGCCGAAAGGCGGCTG‑CGC‑GGGTGGTGGCCCGTTCCCTATAGGCGGTAt > 2:805983/1‑151 (MQ=255) aCGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTTTTGGAATAAAAAAAGGTTTGGGTATTCTGGCGAAACGCGGCTT‑CGC‑CGGTGGGGGCCCGGTCCATATTGGCGTTTTTGAt > 2:803306/1‑151 (MQ=255) cAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTTTTTGAAG‑ACAAAATGTTTTGCGGTACGTGCAGAACGGCGTCTG‑CGC‑GGGTGGGGCCCCGTTCCCTATAGGGGTTATCGATGtt > 2:988585/1‑151 (MQ=255) cAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTG‑CGC‑GGTTGGTGCCCAGTTCCATATAGGCGTTATCGATGtt < 2:565584/151‑1 (MQ=255) aTACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTTTTGAAGTACAAAAAGTTTTGGGGATCGTGCAGAAAGGGGGCTG‑CGC‑GGGTGGGGGCCAGGTTCCCATAAGCGTTTTCGATGttt > 1:428716/1‑149 (MQ=255) aTTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTTTTGAAATAAAGAAAGTTTTGGGGTTCGGGCAGAACGGGGGCTG‑CGC‑GGGTGGGGGCCCGGTCCCTATAAGGGGTATTGATGTTTggggaatg > 1:973868/1‑146 (MQ=255) ttGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTGTTGAAATACAAAAATTTTGGGGGGTCGGGGGGAACGGCGGCCG‑CGC‑GGGTGGTGGCCCCTTTCCTATAAGGGTTATTGATTTTTggggaa > 1:681709/1‑145 (MQ=255) gctgcGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTTATGAAGTACAGAATGTTTTGCGTATCGTGCAGAAAAGCGGCTG‑CGC‑GGGTGGGGCCCCGTTCCATATAGGCGGTATCCATGTTTTGGGACTGAc > 1:1034088/1‑150 (MQ=255) ctgcGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTTGTGAAGTACAGAATGTTTTGGGTATCGTGCCGAACGGCGTCTG‑CGC‑GGGTGGGGGCCCGTTTCCTATAGGGGGTATTGGTGTTTGGGGACTGACg > 2:781592/1‑150 (MQ=255) cgcTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTTGTTAAGGACAAAATGTTTTGCGGATCGTGGAGAAAGGCGTCTG‑CGC‑GGGTGGTGCCCCGTTCCCTATAAGCGGTATTGATGTTTGGGGAATGAc > 1:392173/1‑146 (MQ=255) cgcTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTTGTGAAGAAAAGAAAGTTTTGGGTATCCTGCAGAACAGCGGCTG‑CGC‑GGGTGGGGGCCCGGTCCATATAAGCGTTATCGCGGTTTggggaatg > 2:998775/1‑139 (MQ=255) cgcTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTGATGAAGTACAGAATGTTTGTCGTATCGTGCAGAACGGCGTCTG‑CGC‑GGTTGGTGCCCAGTTCCATATAGGCGTTATCGATGTTCGGGAACTGACGCAGa < 1:643637/151‑1 (MQ=255) gcTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTTGTGAAATACAAAATTTTTTGGGGTTCGTGCAGAACGGGGGCTG‑CGC‑GGGTGGGGGCCCGTTCCCTATAAGAGGTATCGGTGTTTGGGGACTGa > 2:456609/1‑144 (MQ=255) gcTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑CTTTTCTGAAAAACAGAATGTTTTGGGTTTCGTGGAGGACGGCGGCTG‑GGC‑GGGTGGTGGCCCGCTTCACATAGGGGGTTTTGATGTTTggggaat > 1:739555/1‑138 (MQ=255) ttCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTGTTGAAGTAAAGAATGTTTTGGGGATCTTGCAGAACGGGGGCTT‑CGCGGGTGGGGGCCCGGTTCC‑TATATGCGTTATTGATGTTCGGGGACTGACGCCGAt > 2:753551/1‑149 (MQ=255) tCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTTATTAAGTAAAGAAAGTTTTGGGGATTGTGGAGAAAGGCGGCTGCCGG‑GGTGGGGGCCCCGTTCC‑TATAAGGGGTATTGATGTTTGGGGAATGACGCAGAACtt > 2:373782/1‑151 (MQ=255) agagTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTG‑CGC‑GGTTGGTGCCCAGTTCCATATAGGCGTTATCGATGTTCGGGAACTGACGCAGATCTTTAGtt < 1:697344/151‑1 (MQ=255) gagTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTG‑CGC‑GGTTGGTGCCCAGTTCCATATAGGCGTTATCGATGTTCGGGAACTGACGCAGATCTTTAGttt < 1:934054/151‑1 (MQ=255) aCCGCTTTGAACTGACCGTTACCGGCGG‑TTTTTTTGAAGTACAGAATGTTTTGCGGATCGTGCCGAACGGCGGCTG‑CGC‑GGGTGGGGGCCCGTTCCATATAAGGGGTATTGATGTTTGGGGACTGACGCAGATCTTTTGTTTTTATGttt > 2:145292/1‑150 (MQ=255) ttGAACTGACCGTTACCGGCGG‑TTTTTGTTAAATAAAAAATGTTTTGTGTATCGTGCAGAAAGGCGGCCG‑CGC‑GGTTGGTGGCCCGTTCCCTATAAGCGTTATCGATGTTTGGGGAATGGCGCAGAACTTTTGTTTTTATttttttttt > 2:650388/1‑144 (MQ=255) ttGAACTGACCGTTACCGGCGG‑TTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTG‑CGC‑GGTTGGTGCCCAGTTCCATATAGGCGTTATCGATGTTCGGGAACTGACGCAGATCTTTAGTTTTGATGTTTGCttt < 1:1012863/149‑1 (MQ=255) ttGAACTGACCGTTACCGGCGG‑TTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTG‑CGC‑GGTTGGTGCCCAGTTCCATATAGGCGTTATCGATGTTCGGGAACTGACGCAGATCTTTAGTTTTGATGTTTGCTTTc < 1:273077/150‑1 (MQ=255) aaCTGACCGTTACCGGCGG‑TTTTTTTGAAGTAAAGAAAGTTTGGGGGATTGTGCAGAAAGGGGGCTG‑CGC‑GGGTGGGGCCCAGTTCCCTATAGGCGGTATTGATGTTCGGGGAATGACGCCGAACTTTATTTTTTTTGtttttttt > 2:174737/1‑141 (MQ=255) aCTGACCGTTACCGGCGG‑TTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTG‑CGC‑GGTTGGTGCCCAGTTCCATATAGGCGTTATCGATGTTCGGGAACTGACGCAGATCTTTAGTTTTGATGTTTGCTTTCGCGTa < 2:304924/151‑1 (MQ=255) aCCGTTACCGGCGG‑TTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTG‑CGC‑GGTTGGTGCCCAGTTCCATATAGGCGTTATCGATGTTCGGGAACTGACGCAGATCTTTAGTTTTGATGTTTGCTTTCGCGTAATCa < 2:204259/151‑1 (MQ=255) ccGTTACCGGCGG‑TTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTG‑CGC‑GGTTGGTGCCCAGTTCCATATAGGCGTTATCGATGTTCGGGAACTGACGCAGATCTTTAGTTTTGATGTTTGCTTTCGCGTAATCaa < 2:377213/151‑1 (MQ=255) aCCGGCGG‑TTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTG‑CGC‑GGTTGGTGCCCAGTTCCATATAGGCGTTATCGATGTTCGGGAACTGACGCAGATCTTTAGTTTTGATGTTTGCTTTCGCGTAATCAACGGa < 1:355165/150‑1 (MQ=255) cggcgg‑TTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTG‑CGC‑GGTTGGTGCCCAGTTCCATATAGGCGTTATCGATGTTCGGGAACTGACGCAGATCTTTAGTTTTGATGTTTGCTTTCGCGTAATCAACGGAGcc < 2:19808/151‑1 (MQ=255) g‑TTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTG‑CGC‑GGTTGGTGCCCAGTTCCATATAGGCGTTATCGATGTTCGGGAACTGACGCAGATCTTTAGTTTTGATGTTTGCTTTCGCGTAATCAACGGAGCCAGTAc > 2:438303/1‑151 (MQ=255) ttttGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTG‑CGC‑GGTTGGTGCCCAGTTCCATATAGGCGTTATCGATGTTCGGGAACTGACGCAGATCTTTAGTTTTGATGTTTGCTTTCGCGTAATCAACGGAGCCAGTAcc > 1:721337/1‑151 (MQ=255) | TCGTTGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGG‑TTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTG‑CGC‑GGTTGGTGCCCAGTTCCATATAGGCGTTATCGATGTTCGGGAACTGACGCAGATCTTTAGTTTTGATGTTTGCTTTCGCGTAATCAACGGAGCCAGTACC > NC_000913/847295‑847590 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |