Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,097,581	0	A	G	47.1%	90.4 / 30.9	71	noncoding (72/88 nt)	serX	tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (5/32);  new base G (33/0);  total (39/32)
Fisher's exact test for biased strand distribution p-value = 5.23e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGATTCGAACCCCC‑GATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACA‑CCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAA  >  NC_000913/1097438‑1097729                                                                                                                                                  |                                                                                                                                                        cTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGGTTCgct                                                                                                                                                   >  2:404120/1‑148 (MQ=14)     gTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGGTTCGAAccccc                                                                                                                                              >  1:153083/1‑151 (MQ=25)       tCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGATTCGAACCCCC‑g                                                                                                                                            <  2:349474/150‑1 (MQ=37)         aCGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGATTCGAACCCCC‑GATa                                                                                                                                         <  1:263038/151‑1 (MQ=39)          cGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGATTCGAACCCCC‑GATAc                                                                                                                                        <  2:478983/151‑1 (MQ=39)          cGGTCTGTCCAAATGGCTGTCGCCAGTTGGCCACCCCCGGGCGGTGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGATTCGAACCCCC‑GATAc                                                                                                                                        <  2:141762/151‑1 (MQ=14)           ggTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGGATCGGACCCCC‑CATAc                                                                                                                                        >  1:481210/1‑150 (MQ=255)             tCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGGTTCGGAccccccctaagtt                                                                                                                                      >  1:1012859/1‑143 (MQ=255)             tCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGGGG‑GGGGGGGGGTCGAAccccccacaccg                                                                                                                                       >  2:434278/1‑143 (MQ=11)                gTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGGTTGGATcccccccccc                                                                                                                                         >  2:419004/1‑140 (MQ=255)                gTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGGTTAGGAACCCC‑CAGACGTTGc                                                                                                                                   >  1:137365/1‑150 (MQ=11)                gTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGATTCGAACCCCC‑GATACGTTg                                                                                                                                    <  1:889408/149‑1 (MQ=255)                    aaCTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGATTCGAACCCCC‑GATACGTTGCCGtat                                                                                                                              <  2:1047965/151‑1 (MQ=255)                     aCTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTCA‑GGGGGGGATTCTCACCCCC‑CAtcagcttc                                                                                                                                   >  2:359133/1‑138 (MQ=11)                      cTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGG‑GGGGGGGGTTCGAAACCCC‑CATACGTTTCCGtat                                                                                                                              >  1:854171/1‑149 (MQ=255)                       tGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGG‑GGGGGGGGTTTGAAACCCC‑GCTACGTTTCCCtat                                                                                                                              >  2:840612/1‑148 (MQ=11)                       tGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGGTTCGAACCCCC‑CCTAAATTGCCCtat                                                                                                                              >  2:363384/1‑148 (MQ=11)                        ggCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGATTCGAACCCCC‑GATACGTTGCCGTATaca                                                                                                                           <  1:404137/150‑1 (MQ=255)                           tGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGGTTCGAACCCCC‑CCTACGTTTCCGta                                                                                                                               >  1:340534/1‑143 (MQ=255)                              cGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGATTCGAACCCCC‑GATACGTTGCCGTATACACA‑Cttt                                                                                                                    <  2:386152/150‑1 (MQ=255)                               gCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGATTCGAACCCCC‑GATACGTTGCCGTATACACA‑CTTTcc                                                                                                                  <  1:853989/151‑1 (MQ=255)                                     tGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGATTCGAACCCCC‑GATACGTTGCCGTATACACA‑CTTTCCAGGCg                                                                                                             <  1:278087/150‑1 (MQ=255)                                            ccccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGATTCGAACCCCC‑GATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCtt                                                                                                     <  2:204898/151‑1 (MQ=255)                                               cGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGAGGGGGGGGGTTGGAACCCCC‑‑ATACGTTGCCGTATACACA‑CTTTTCCGGGGTGGTCCtt                                                                                                     >  1:444288/1‑148 (MQ=14)                                               cGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGGGTTGAACCCCC‑CCTACGGTGCCGTATACACA‑CCTTCCAGGCggggccc                                                                                                       >  2:20557/1‑140 (MQ=14)                                               cGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGGGG‑GGGGGGGGTTCGAAACCCC‑CATACGATTCCGGATACACA‑CTTTTCCGGCGGGCTCCtt                                                                                                     >  2:839864/1‑148 (MQ=17)                                                 gTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGATTCGAACCCCC‑GATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGcc                                                                                                <  1:96155/151‑1 (MQ=255)                                                  tCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGGTTTGAAACCCC‑CCTACGGTGCCGTATACACACCTTTCCA‑GCGGGCTCCTTCCGcc                                                                                                >  1:395888/1‑150 (MQ=14)                                                    ggggCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGGTTCGAACCCCC‑CATACGTTGCCCGATACACA‑CTTTTCACGCGTGCCCCTTCAGCCAc                                                                                              >  1:1021203/1‑150 (MQ=18)                                                    ggggCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGGTTCGAAACCCC‑CATACGTTTCCGTATACACA‑CTTTCCACGCGGGCTCCCTCAGCCAc                                                                                              >  1:768050/1‑150 (MQ=18)                                                      ggCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGGTTCCAACCCCC‑CATACGTTTTCGTATACACA‑CTTTTCCGGCGTGCCCCTTCAGcc                                                                                                >  1:758099/1‑146 (MQ=17)                                                        cTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGGTTCGAAACCCC‑CACACGTTGCCCTATAAAAA‑CTTTTCACGCGTGGCCCTTctccccccccg                                                                                          >  2:613043/1‑140 (MQ=14)                                                          tctcATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGGGTTGAACCCCCGGATA‑GTTGGCCTATAAACA‑CTTTTCCGGCGGGGTCCTTCTGcccccccggc                                                                                        >  1:548636/1‑142 (MQ=14)                                                           ctcATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGGG‑GGGGGGGGTTGGAACCCCC‑‑ACAAGTTGCCGCATACACA‑CCTTTCCGGGGGGCTCCCTCCGcccccccggcac                                                                                      >  2:835328/1‑141 (MQ=2)                                                               tCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGGGTCGACCCCCC‑GATAAGTTGCCCGTTAAACA‑CTTTCCAGGCGTGCTCCTTCAGCCACCCGGacaac                                                                                     >  1:648729/1‑146 (MQ=17)                                                                ccccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGATTCGAACCCCC‑GATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACA‑CCTCa                                                                                 <  2:987938/150‑1 (MQ=255)                                                                   cccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGATTCGAACCCCC‑GATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACA‑CCTCACCaa                                                                             <  2:1053779/151‑1 (MQ=255)                                                                     cGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGGGTCCAACCCCC‑CATACGTTGCCGTATACACA‑CTTTTCACGCGTGCTCCCTCCGCCCCCCCGGCA‑CCCCAcc                                                                               >  1:718265/1‑147 (MQ=14)                                                                      ggTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGGTTCGGACCCCC‑CCTACGTTGTCGTATAAACA‑CTTTTCAGGCGGGCTCCTTCCGCCACCCCGACC‑CCCCACCAAAtt                                                                          >  1:498049/1‑151 (MQ=14)                                                                         gtgtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGATTCGAACCCCC‑GATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACA‑CCTCACCAAATTGt                                                                        <  1:130444/150‑1 (MQ=255)                                                                           gtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGATTCGAACCCCC‑GATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACA‑CCTCACCAAATTGTCGt                                                                     <  1:628629/151‑1 (MQ=255)                                                                               aaTATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGGGTCGAAACCCC‑CCCACGTTGTCGTATACACA‑CCTTCCAGGCGCGGCCCTTCAGCCCCCCCGACA‑CCTCACCCAATTTTCGTTcc                                                                  >  2:1065059/1‑150 (MQ=14)                                                                               aaTATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGATTCGAACCCCC‑GATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACA‑CCTCACCAAATTGTCGTTCCt                                                                 <  2:815847/151‑1 (MQ=255)                                                                                   taCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGGTTCGTAACCAC‑CATACGTTTCTGTCTAAACA‑ACTTCCAGGCGGGCGGCTTCATCCCCTCTGGCA‑CCTCACCCAATTTTCCtt                                                                    >  2:419024/1‑144 (MQ=2)                                                                                    aCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGAGGGGGGGGATTTAAACCCCC‑‑ATACGGTGCCCCGTAAAAA‑CTTTTCCGGCGTGCGCCTTCAGCCACACGGGCA‑CCTCACCAAATTTTCGTTTCCGTc                                                              >  1:447296/1‑149 (MQ=2)                                                                                      gAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGATTCGAACCCCC‑GATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACA‑CCTCACCAAATTGTCGTTCCTGTCTTGc                                                          <  2:577952/151‑1 (MQ=255)                                                                                      gAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGATTCGAACCCCC‑GATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACA‑CCTCACCAAATTGTCGTTCCTGTCTTGc                                                          <  1:745613/151‑1 (MQ=255)                                                                                       aaaaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGATTCGAACCCCC‑GATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACA‑CCTCACCAAATTGTCGTTCCTGTCTTGc                                                          <  1:955651/150‑1 (MQ=255)                                                                                         aaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGG‑GGGGGGGGTTCGAAACCCC‑CCTACGTTTTCGTATACACA‑CTTTTCCGGCGGGCTCCCTTAGCCCCTCGGGCA‑CCTCACCCAATTTTCGTTCCTGTCtt                                                            >  1:43771/1‑146 (MQ=14)                                                                                                   gTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGGTTCGAACCCCC‑CATACGTTTCCGTATTAACA‑ACTTTCAGGCGTGGTCCTTCAGCCACCCGGACC‑CCCCCCCAAATTTTTGTTTCTGTCTTTCTGGGACGGgcg                                               >  1:675173/1‑149 (MQ=14)                                                                                                    tACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGGTTTGAACCCCC‑CATACGTTTCCGGATAAACA‑CTTTCCAGGGGTGCTCCTTCAGCCACCCGGACA‑CCCCCCCCAATTGTCGTTCCTGTCTTGCTGGAACgg                                                  >  2:443043/1‑145 (MQ=17)                                                                                                      cTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGGTTCTAACCCCC‑CCTACGTTGCCGTCTAAACA‑CTTTTCAGGCGTGCTCCTTCCGCCACCCCGGCACCCCCCCCAAATTTTGTTTCCT‑TCTTTCTGGGACGGGCGCCaa                                           >  2:774962/1‑150 (MQ=2)                                                                                                      cTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGATTCGAACCCCC‑GATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACA‑CCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTa                                            <  2:504912/149‑1 (MQ=255)                                                                                                       tttCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGGGTTGAACCCCC‑CCTACGTTTTCGGATACACA‑CTTTCCAGGCGGGCTCCCTCAACCCCCCCGGCA‑CCCCACCCAAATTTTGTTCCTGTCTTTTTGGGACGGGCGCCAAtt                                         >  1:380494/1‑151 (MQ=9)                                                                                                          cGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGATTCGAACCCCC‑GATACGTTGCCGTATACACT‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACA‑CCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAg                                      <  2:685025/151‑1 (MQ=255)                                                                                                          cGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGATTCGAACCCCC‑GATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACA‑CCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAg                                      <  2:726329/151‑1 (MQ=255)                                                                                                               gAGCTCTTCTTTAAATATGGC‑GGTTA‑GGGGGGGGGTTGAACCCCC‑CGTAAGTTTTCGTATAGACA‑CTTTTCACGGGTGCTCCTTTCCACACCCCGACA‑CCCCCCCCAATTTTTGTTTCTGTCTTTCTCGAACCGgccgtaacttttggta                                  >  1:69590/1‑136 (MQ=2)                                                                                                               gAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGATTCGAACCCCC‑GATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACA‑CCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGaaa                                 <  1:419022/151‑1 (MQ=255)                                                                                                                     ttcttTAAATATGGC‑GGTGAGGGGGGGGTTTGGAACCCCC‑G‑TAAGGTGCCGGATACACA‑CCTTTCAGGCGGGCCCCCTCCGCCACCCGGACA‑CCCCCCCCAAATTTTGTTTCTTTCTTTCTGGAACGGGGGCCAAtttttgggaa                                 >  1:528538/1‑138 (MQ=2)                                                                                                                        tttAAATATGGC‑GGTGA‑GGGGGGGGGTTGAACCCCC‑CATACGTTGCCGGATAAACA‑CTTTTCCGGCGGGCTCCTTCAGCCCCCCGGACA‑CCCCCCCCAAATTTCGTTCCTGTCTTTCTGGAACGGGCGCCAATTTAGGGGAATCATGAcc                         >  1:557454/1‑150 (MQ=18)                                                                                                                         ttAAATATGGC‑GGTGA‑GGGGGGGATTCGAACCCCC‑GATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACA‑CCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACTGGCGCTAATTTAGGGAAATCATGAcc                         <  2:472099/149‑1 (MQ=255)                                                                                                                         ttAAATATGGC‑GGTGA‑GGGGGGGATTCGAACCCCC‑GATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACA‑CCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGAc                          <  1:370038/148‑1 (MQ=255)                                                                                                                         ttAAATATGGC‑GGTGA‑GGGGGGGATTCGAACCCCC‑GATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACA‑CCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGAc                          <  1:404074/148‑1 (MQ=255)                                                                                                                               atGGC‑GGTGA‑GGGGGGGATTCGAACCCCC‑GATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACA‑CCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTc                  <  1:1067544/150‑1 (MQ=255)                                                                                                                                  gC‑GGTGA‑GGGGGGGATTCGAACCCCC‑GATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACA‑CCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTcaaca              <  1:467593/151‑1 (MQ=255)                                                                                                                                     ggTGA‑GGGGGGGATTCGAACCCCC‑GATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACA‑CCTCACAAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACaaa            <  2:392446/151‑1 (MQ=255)                                                                                                                                        gA‑GGGGGGGATTCGAACCCCC‑GATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACA‑CCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACtt         >  1:174233/1‑151 (MQ=255)                                                                                                                                             gggggATTCGAACCCCC‑GATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACA‑CCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTg     <  2:1042418/151‑1 (MQ=255)                                                                                                                                                ggATTCGAACCCCC‑GATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACA‑CCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGaaa  <  2:768022/151‑1 (MQ=255)                                                                                                                                                ggATTCGAACCCCC‑GATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACA‑CCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGaa   >  1:973976/1‑150 (MQ=255)                                                                                                                                                ggATTCGAACCCCC‑GATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACA‑CCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGaa   >  2:261912/1‑150 (MQ=255)                                                                                                                                                  |                                                                                                                                                        CTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGC‑GGTGA‑GGGGGGGATTCGAACCCCC‑GATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACA‑CCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAA  >  NC_000913/1097438‑1097729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑