Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,001,9110GA43.8% 52.0 / 27.5 48intergenic (‑122/+199)fliA/fliCRNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein (flagellin)
Reads supporting (aligned to +/- strand):  ref base G (26/1);  new base A (0/21);  total (26/22)
Fisher's exact test for biased strand distribution p-value = 9.86e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGC  >  NC_000913/2001770‑2002059
                                                                                                                                              |                                                                                                                                                    
gCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAAcc                                                                                                                                             >  2:169736/1‑151 (MQ=255)
       agagTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCgg                                                                                                                                      >  2:185269/1‑151 (MQ=255)
       agagTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCgg                                                                                                                                      >  1:699147/1‑151 (MQ=255)
             aaTTCACGATAAACAGCCCTGCGTTATATGATTTATCGGCATGATTATCCTTTTCTGCGGGTTTTTTAATCGGACGATTAGGGGGTGAAATGAGGGTTTATTGGGGGGTTACAGGTAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCgg                                                                                                                                <  1:73108/151‑1 (MQ=255)
                   cGATAAACAGCCCTGCTTTATATGAGTTACCGGCATGATTATCCTTTTCTGCAGGTTTTTAAACCGGACGATTAGTGGGTAAAATGGGGGGTTATTGGGGGGTTACAGGTAAATTCCGGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAg                                                                                                                           <  1:189267/150‑1 (MQ=255)
                     aTAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCAc                                                                                                                        >  1:825444/1‑151 (MQ=255)
                     aTAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCAc                                                                                                                        >  1:466848/1‑151 (MQ=255)
                       aaaCAGCCCTGCGTTATAGGATTTAGCGGAATGTTTTTCGGTTTCTGGGGGGTTTTAAATCGGACAATTGGGGGGTAAAAGGGGGGGTTTTTTGGGGGTTACAGTTAAATCCCGGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCAc                                                                                                                        <  2:152219/149‑1 (MQ=255)
                        aaCAGCCCTGCTTTATATGAGTTATCGGCATGTTTATCCTTTTCTGCGGGGTTTTTAATCGGACGATTAGTGGGTGAAATGGGGGGTTTTTGGGGGTTTACGGGTAAATCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGtt                                                                                                                     <  1:540590/151‑1 (MQ=255)
                           aGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCt                                                                                                                  >  2:499673/1‑151 (MQ=255)
                             cccTGGGTTTTATGATTTTGCGGCTTGATTACCCGTTTCTGGGGGGTTTTTAATCTGACGATTGGGGGGAAAAATGAGGGTTTTTTGGGGGTTTACAGGAAAATTCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGa                                                                                                                <  1:1088925/151‑1 (MQ=255)
                             cccTGCTTTATATGATTTATCGGCATGTTTATCCTTTTCTGCAGGTTTTTAAATCGGACGATTAGGGGGTAAAATGAGGGTTTTTTTGGGGTTTACAGTTAAATTCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTg                                                                                                                 <  2:13325/150‑1 (MQ=255)
                                    ttATATGAGTTATCGGCATATTTATCCGTTTCTGCGGGTTTTTTAATCGGACGATTAGGGGGTAAAATGGGGGTTTTTTGGGGGGTTACAGGTAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACaaa                                                                                                            <  1:393355/148‑1 (MQ=255)
                                             ttacgggcttaaTTTTCCGTTTCTGCGGTTTTTTAAATCGAACGATGGGGGGGTGAAAGGGGGGTTTTTTGGGGGGTAAAAGGTAAATTCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCttt                                                                                                   <  2:1037275/137‑1 (MQ=255)
                                              tagcGGCATGATTATCCGTTTCTGCAGGTTTTTAAATCGGACGATTAGGGGGTAAAATGAGGGGTTTTTTGGGGGTGACAGGAAAATCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATg                                                                                                <  2:896836/147‑1 (MQ=255)
                                                     aTGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCgg                                                                                         >  1:1023072/1‑150 (MQ=255)
                                                     aTGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGAAAATTCCAGGCAGAAAAAAACACCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCgg                                                                                         >  1:153407/1‑150 (MQ=255)
                                                       gATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATg                                                                                      >  2:78352/1‑151 (MQ=255)
                                                         ttATCCTTTTCTGCGGGTTTTTTAATCGGACGATTGGGGGGTAAAAGGGGGGTTTATTTGGGGGTTACAGGTAAATTCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgc                                                                                     <  2:389777/150‑1 (MQ=255)
                                                         ttATCCGTTTCTGCAGGTTTTTTAATCGGACGATTAGTGGGTAAAAGGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgc                                                                                     <  2:258275/150‑1 (MQ=255)
                                                           aTCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgcggc                                                                                  >  1:705440/1‑151 (MQ=255)
                                                           aTCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgcggc                                                                                  >  1:550314/1‑151 (MQ=255)
                                                             ccttttCTGCGGGTTTTTTAATGGGACGTTTGGGGGGTAAAAGGAGGGTTTTTTGGGGGTTTACAGGTAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgcggcg                                                                                 <  1:609406/147‑1 (MQ=255)
                                                                ttccggaggggTTTGTAAGCGGTCAATTAGTCGTAGAACAATGGGTTTATTGGGGGCTTCCAGGGAAGTCCCATCCAAAAAAAAGCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgcgg                                                                                   <  1:244070/137‑1 (MQ=255)
                                                                 ttCTGCGGGGTTTTTAATCGGACAATTAGTGGGTGAAAGGGGGGGTTTTTGGGGGGTTACAGGAAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTaaa                                                                             <  1:977828/150‑1 (MQ=255)
                                                                    tGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGcc                                                                         >  1:518978/1‑151 (MQ=255)
                                                                        gggTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTAt                                                                     >  2:392704/1‑151 (MQ=255)
                                                                             tttAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                >  1:355208/1‑151 (MQ=255)
                                                                                aaTCGGACGATTAGGGGGTAAAATGGGGGTTTTTTTGGGGGTTAAAGGTAAATCCCAGGAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc                                                            <  2:260220/151‑1 (MQ=37)
                                                                                 acgggaggtttggTGGGGAAAATGGGGGTTTTTTTGGGGGTTCCAGAAAAATCCCGGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc                                                            <  2:836687/139‑1 (MQ=25)
                                                                                    ggACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaa                                                          >  1:1052019/1‑150 (MQ=255)
                                                                                    ggACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaa                                                          >  1:321577/1‑150 (MQ=255)
                                                                                    ggACGATTAGGGGGTGAAAGGGGGGTTTTTTTGGGGGTTAAAGGAAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaa                                                          <  2:368889/150‑1 (MQ=37)
                                                                                         aTTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtg                                                     >  1:194312/1‑150 (MQ=255)
                                                                                          ttAGGGGGTGAAAGGAGGGGTTATTGGGGGGTTACAGTTAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtgt                                                    <  1:39751/150‑1 (MQ=38)
                                                                                               gggTAAAAGGGGGGTTTTTTTGGGGTTTACAGGTAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCa                                                 <  2:343042/148‑1 (MQ=37)
                                                                                                 gTGAAATGAGGGGTTATTTGGGGATTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTc                                            >  2:34419/1‑151 (MQ=255)
                                                                                                    aaaTGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAAt                                         >  2:859223/1‑151 (MQ=255)
                                                                                                      aTGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATa                                        >  2:355415/1‑150 (MQ=255)
                                                                                                         gggggTTTTTTGGGGGGTTACAGGAAAATCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATaa                                       <  1:884312/148‑1 (MQ=37)
                                                                                                                 tttGGGGGTTACAGGTAAATCCCAGGCAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAtt                              <  1:327745/149‑1 (MQ=255)
                                                                                                                      gggtTTACAGTAAAATCCCGGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACtt                        <  1:78345/151‑1 (MQ=37)
                                                                                                                             cAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGcc                 >  1:193294/1‑151 (MQ=255)
                                                                                                                               ggTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTg               >  1:921776/1‑151 (MQ=255)
                                                                                                                                   aaTTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATa            >  2:301037/1‑150 (MQ=255)
                                                                                                                                   aaTTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATa            >  1:864936/1‑150 (MQ=255)
                                                                                                                                         aGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgc       >  2:193067/1‑149 (MQ=255)
                                                                                                                                             aGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCATTTCAACTTGTAGGCCTGATAagcgcagcgc  >  1:69087/1‑150 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
GCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGC  >  NC_000913/2001770‑2002059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: