Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,270,455	0	G	T	42.2%	92.9 / 29.5	64	intergenic (+853/+170)	yhaC/garK	pentapetide repeats‑related protein/glycerate kinase I
Reads supporting (aligned to +/- strand):  ref base G (6/31);  new base T (27/0);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 1.35e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

ACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCA‑C‑TGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATA  >  NC_000913/3270312‑3270605                                                                                                                                                |                                                                                                                                                          aCGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGctctct                                                                                                                                                     <  2:718617/150‑1 (MQ=255)    ggCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGCTTGCTCTCTGtt                                                                                                                                                  >  1:456828/1‑150 (MQ=255)     gCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGc                                                                                                                                                <  2:381638/151‑1 (MQ=255)       cGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTTTCTCTGttttac                                                                                                                                              >  2:726290/1‑147 (MQ=255)        gTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCA‑C‑t                                                                                                                                           <  1:12075/151‑1 (MQ=255)         tACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCCCTGTTTCA‑C‑t                                                                                                                                           >  1:523438/1‑150 (MQ=255)              tATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCA‑C‑TGGTc                                                                                                                                       <  2:406510/149‑1 (MQ=255)                 gAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCA‑C‑TGGTCGT‑gg                                                                                                                                  <  1:115985/150‑1 (MQ=255)                    ccccTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCA‑C‑TGGTCGT‑GGGttt                                                                                                                              <  1:824267/151‑1 (MQ=255)                       cTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGCTTTCTCTCTTTTGCC‑C‑CGGTCGT‑GGGGTTccc                                                                                                                           >  2:633544/1‑151 (MQ=255)                           ttGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTGCA‑C‑TGGTCGT‑GGGGTTtcccccc                                                                                                                       >  1:15573/1‑151 (MQ=255)                           ttGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCA‑C‑TGGTCGT‑GGGTTTcccccc                                                                                                                        <  2:549842/150‑1 (MQ=255)                            tGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTTTTTTGCA‑C‑CCGGCGT‑GGGGTTcccccccc                                                                                                                      >  2:988271/1‑150 (MQ=255)                            tGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTTTTGCA‑C‑CTGTCCTGGGGTTTccccccc                                                                                                                       >  2:462540/1‑151 (MQ=255)                                 ttGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTTCACC‑TGGCCGG‑GGGTTTccccccccgc                                                                                                                    >  1:945669/1‑146 (MQ=255)                                      ccGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTTCCTTGCA‑C‑CGGTCGT‑GGGGTTTCCCCCCCCGCGTTTcc                                                                                                             >  2:968628/1‑150 (MQ=255)                                      ccGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCA‑C‑TGGTCGT‑GGGTTTCCCCCCCAGGCGTTAcc                                                                                                             <  2:648354/150‑1 (MQ=255)                                        gggTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCA‑C‑TGGTCGG‑GGGTTTcccccccccgcctgccctgg                                                                                                          >  2:770193/1‑138 (MQ=255)                                         ggTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTGTCTCTGTTGCA‑C‑TGGTCGA‑GGGGTTTCCCCCCCCGCGTTTcc                                                                                                             >  1:472289/1‑147 (MQ=255)                                          gTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCA‑C‑TGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCa                                                                                                        <  2:979529/151‑1 (MQ=255)                                          gTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCA‑C‑TGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCa                                                                                                        <  2:594290/151‑1 (MQ=255)                                                 ttACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTTCA‑C‑TGGCCGG‑GGGGTTTCCCCCCCGGCGTTTCCCGGCACCCCGcc                                                                                                 >  2:1082784/1‑151 (MQ=255)                                                  tACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCGCCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCA‑C‑TGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGcc                                                                                                 <  1:982548/150‑1 (MQ=255)                                                   aCCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTGTA‑C‑TGGTCGG‑GGGGTTTCCCCCCCGGCGGTTACTGGCCCCCTGccc                                                                                                >  1:368993/1‑150 (MQ=255)                                                    ccGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCCTTTGCA‑C‑TGGGCGTGGGGGTCCCCCCCCCGGCGTTAcccgcccccccccc                                                                                                 >  2:493003/1‑137 (MQ=255)                                                    ccGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCA‑C‑TGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTa                                                                                              <  1:247191/151‑1 (MQ=255)                                                    ccGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCA‑C‑TGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTa                                                                                              <  1:498480/151‑1 (MQ=255)                                                        gCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCGCCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCA‑C‑TGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGa                                                                                          <  1:893075/151‑1 (MQ=255)                                                           aCGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCCCTCCGTTTAA‑C‑AGGGGAA‑GGGGTTCCCCCCCAGCCCGTACCCGGCGACCTGCCCttctgcgcc                                                                                       >  1:626214/1‑143 (MQ=255)                                                           aCGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCA‑C‑TGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGc                                                                                        <  1:462686/150‑1 (MQ=255)                                                             ggACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTTCA‑C‑TGGTCGT‑GGGGTTCCCCCCCCGGCGTTACCTGGCACCCTGCCCCATTGAGccc                                                                                      >  1:862062/1‑150 (MQ=255)                                                             ggACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCA‑C‑TGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCg                                                                                     <  1:681071/151‑1 (MQ=255)                                                               aCTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCA‑C‑TGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGa                                                                                   <  2:367514/151‑1 (MQ=255)                                                                   ttACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTTCA‑C‑TGGTCGT‑GGGGTTCCCCCCCCGGCGGTTCCTTGCACCCCGCCCCATTGGGCCCCGGCttt                                                                               >  1:1102470/1‑151 (MQ=255)                                                                     aCCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTTTCTCTGTTTCC‑C‑TGGGCGT‑GGGGTTTCCCCCCCCGCGGTTCCTTGCACCCCGCCCCCTGGGGCCCCGACttt                                                                               >  2:683894/1‑149 (MQ=255)                                                                     aCCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTTTCTCTGTTGCA‑C‑TGGTCGT‑GGGGTTCCCCCCCCGGCGTTACCCGGCACCCCGCCCTATGGAGCCCGGACTTtcc                                                                             >  1:661372/1‑151 (MQ=255)                                                                     aCCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCA‑C‑TGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTtcc                                                                             <  2:1005948/151‑1 (MQ=255)                                                                     aCCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCA‑C‑TGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTtcc                                                                             <  1:652408/151‑1 (MQ=255)                                                                     aCCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGATTTCTCTCTTTTGCA‑C‑CGGTGGC‑GGGGTTTCCCCCCCCGCGGTTCCTCGCACCCCGCCCCATGGAGCCCGGGCttt                                                                               >  1:164889/1‑149 (MQ=255)                                                                      ccAGCCGCGCGGTGCGCTCTTCCCGCACCTTTTCCCCCTTCCCGGATCCCGCTTGCGCGGGCCACCGCCGGTTTGCTTCCTGTTGCA‑C‑TGGTCGT‑GGGTTT‑CCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTtcct                                                                            <  1:173595/150‑1 (MQ=255)                                                                       cAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTTTTGCC‑C‑TGGGCGT‑GGGGTTCCCCCCCCCGCGTTACCTTGCCCCCCGCCCCTTGGAGACCCGGCttt                                                                               >  2:136685/1‑147 (MQ=255)                                                                             cgcgGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCA‑C‑TGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTcctcccctcc                                                                     <  2:554933/151‑1 (MQ=255)                                                                             cgcgGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGCTTTCTCCCTTTTGCC‑CACGTTCGC‑GGGTTTCCCCCCC‑CCCGCTACCTGGCACCCCTCCCCCTTGACCCCGGCGTTTtctcccccc                                                                      >  2:977135/1‑148 (MQ=255)                                                                                 gTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCA‑C‑TGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGccc                                                                 <  1:27940/151‑1 (MQ=255)                                                                                        ctTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGGTTTGTCCCCATTTCG‑C‑CAGGCGG‑GGGGTTTCCCCCCCGGCGTTTCCCTCGACCCTCCCCTTTGGTGTCCCGCCTTTTCCCCCCCCCTccc                                                                 >  1:586044/1‑144 (MQ=255)                                                                                         ttACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTTTTTCA‑C‑TGGTTGT‑GGGGTTTCCCCCCCGGCGGTTCCCTGCCCCCTGCCCTATTGGGCCCGGGCTTTTCTCCCCCCCCCCCCGCTcccc                                                         >  1:918478/1‑151 (MQ=255)                                                                                           aCCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCA‑C‑TGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTccccc                                                        <  1:788485/150‑1 (MQ=255)                                                                                             cGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTTTTGCC‑C‑TGGTCCT‑GGGGTTTCCCCCCCGGCGGTACCTGGCCCCCCGCCCTTTTGAGCCCCGGCTTTCCTCCCCCCCCCCCCCCTcccccc                                                       >  2:747784/1‑148 (MQ=255)                                                                                                     ttCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCA‑C‑TGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGgac                                               <  1:774025/149‑1 (MQ=255)                                                                                                      tCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCA‑C‑TGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGgacga                                             <  2:208004/150‑1 (MQ=255)                                                                                                             tACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCA‑C‑TGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCGCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGaa                                      <  1:177367/150‑1 (MQ=255)                                                                                                               ccTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCA‑C‑TGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAgc                                    <  1:988309/150‑1 (MQ=255)                                                                                                                 tGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTTTTGCA‑C‑CGGTCGT‑GGGGTTCCCCCCCACCCGGTTCCTGGCCCCCCGCCCCCTGGAGCCCGGGCTTTCCTCCCCCCCCCCCCGCTCCCCCCCAGAGGacgacgacgcaaagg                                  >  2:1085636/1‑144 (MQ=255)                                                                                                                   aTCCCGCTTGCGCGGGCCATCGGCGGTTTTTTTTCTGTTTCA‑C‑TGGGCGG‑GGGGTTTCCCCCCCGGCGGTTCCCGGGACCCCGCCCTCTGGGGCCCGGACTTTCCCCCCCCCCCCCCCCCCCCCCCGCaga                                                   >  2:644809/1‑131 (MQ=255)                                                                                                                    tCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTTCA‑C‑TGGTCGG‑GGGGTTCCCCCCCCCGCGGTACCTGGCCCCCCGCCCCATGGGGGCCCGCCCTTTCTCCCCCCCCCCCCCCTCCCCCCCAGAAGACGACGGCGAAacggcg                                >  1:592194/1‑149 (MQ=255)                                                                                                                        gCTTGCGCGGGCCATCGGCGGTTTTTTCTCTGTTGCA‑C‑CGGTCGT‑GGGGTTTCCCCCCAGGCGTTTCCTGGCACCCCGCCCCTTGGGGCCCGGACTTTTCTCCCCCCCCCCCCTCTCCCCCCAAGGGGGCGACGACGAAGCGGCGAc                              >  1:535050/1‑147 (MQ=255)                                                                                                                          ttGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCA‑C‑TGGTCGT‑GGGTTT‑CCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGt                           <  2:55254/147‑1 (MQ=255)                                                                                                                           tGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCA‑C‑TGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCt                         <  2:1049802/149‑1 (MQ=255)                                                                                                                             cgcgGGCCATCGGCGGTTTGCTCTCTGTTGCA‑C‑TGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTTGTc                     <  1:159854/151‑1 (MQ=255)                                                                                                                                  gCCATCGGCGGTTTGCTCTCTGTTGCA‑C‑TGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCtt                <  1:700886/151‑1 (MQ=255)                                                                                                                                      tCGGCGGTTTGCTCTCTGTTGCA‑C‑TGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGgc            >  2:706339/1‑151 (MQ=255)                                                                                                                                          cggTTTGCTCTCTGTTGCA‑C‑TGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGgcgcgc        >  2:178544/1‑151 (MQ=255)                                                                                                                                            gTTTGCTCTCTGTTGCA‑C‑TGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGgcgcgc        >  2:804674/1‑149 (MQ=255)                                                                                                                                             tttGCTCTCTGTTGCA‑C‑TGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGt     >  1:717345/1‑151 (MQ=255)                                                                                                                                                gCTCTCTGTTGCA‑C‑TGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGtata  <  1:984481/151‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                          ACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCA‑C‑TGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTATA  >  NC_000913/3270312‑3270605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑