Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,3440CT47.2% 54.5 / 49.8 72S62F (TCT→TTT) bglJbgl operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (8/30);  new base T (34/0);  total (42/30)
Fisher's exact test for biased strand distribution p-value = 3.62e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAC  >  NC_000913/4604199‑4604492
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aTAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttcttttt                                                                                                                                                  <  2:1061704/151‑1 (MQ=255)
   gAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTttttttttttct                                                                                                                                                >  2:1025406/1‑150 (MQ=255)
     aaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGcc                                                                                                                                             <  2:392638/151‑1 (MQ=255)
       aaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGcc                                                                                                                                             >  1:523433/1‑149 (MQ=255)
            gTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCACTTTTTTTTTTTTTTCCAt                                                                                                                                           >  2:987024/1‑146 (MQ=255)
             tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCTTTTTTTCTTTTTTTTCCATgag                                                                                                                                        >  1:223090/1‑148 (MQ=255)
             tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCAt                                                                                                                                           >  1:83885/1‑145 (MQ=255)
             tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATg                                                                                                                                          >  1:1041296/1‑146 (MQ=255)
              cATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGaa                                                                                                                                      >  2:690592/1‑149 (MQ=255)
              cATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGGGaa                                                                                                                                      >  1:761266/1‑149 (MQ=255)
              cATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAg                                                                                                                                     <  1:407531/150‑1 (MQ=255)
               aTGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTg                                                                                                                                   >  2:451111/1‑151 (MQ=255)
                tGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGaa                                                                                                                                      >  2:515339/1‑147 (MQ=255)
                tGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCATGAGAAGTg                                                                                                                                   >  2:515519/1‑150 (MQ=255)
                     agtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTGGCCTGAGAAGGGa                                                                                                                                  >  1:197130/1‑146 (MQ=255)
                     agtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgcgc                                                                                                                              <  1:71419/150‑1 (MQ=255)
                     agtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgcgc                                                                                                                              <  2:273410/150‑1 (MQ=255)
                          cGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCAAGAGAAGTGAg                                                                                                                                 >  2:117012/1‑142 (MQ=255)
                             tATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCAGAGAggg                                                                                                                      >  2:538305/1‑150 (MQ=255)
                                tGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAGCGCAGAGAGGGAtt                                                                                                                   >  1:387248/1‑150 (MQ=255)
                                tGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGGATTTTTTTTTTTTTTGTCATTGGGAGTGAGGGCAGAGGGGGAtt                                                                                                                   >  2:356896/1‑150 (MQ=255)
                                 gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTa                                                                                                                  <  1:231911/150‑1 (MQ=255)
                                    agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCtt                                                                                                              <  2:922775/151‑1 (MQ=255)
                                    agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCtt                                                                                                              <  2:231267/151‑1 (MQ=255)
                                       ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGc                                                                                                            <  1:704090/150‑1 (MQ=255)
                                         aTTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCct                                                                                                          <  2:708836/150‑1 (MQ=255)
                                            cAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCctgact                                                                                                      <  2:219891/151‑1 (MQ=255)
                                             aGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCctgactg                                                                                                     <  1:971846/151‑1 (MQ=255)
                                              gAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGGGGGGGTATCTTTCctgac                                                                                                       >  2:88991/1‑148 (MQ=255)
                                               aaaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGaa                                                                                                   <  1:881476/151‑1 (MQ=255)
                                                    ttGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTgg                                                                                               <  2:574870/150‑1 (MQ=255)
                                                      gCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTCTGCCATGAGAAGGGAGCGCAGAGAGGGGTTTTTTTTCCTGACTGaaactg                                                                                               >  1:398805/1‑144 (MQ=255)
                                                       cGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTCTGCCATGGGAAGGGGGGGCCGAGGGGGGTTTTCTTGACTGACTGAACTGGGGAt                                                                                          >  2:819319/1‑151 (MQ=255)
                                                        gggTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTTCCCTGGGGAGTGAGCGCAGAGAGGGATTTTTTTGCCTGACTGAACTgggg                                                                                             >  2:794718/1‑146 (MQ=255)
                                                        gggTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGAtt                                                                                         <  1:155229/151‑1 (MQ=255)
                                                           tAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCAGGGAGGGGTTATTTTTCCTGCCTTAAATGGCGAtt                                                                                         >  2:343665/1‑148 (MQ=255)
                                                               ccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTTGCATGGGAAGTGGGCGCCGAGGGGGGTTTTTTTTGCTGTCTGAACTGGCGGTTAAGgtt                                                                                   >  2:458803/1‑150 (MQ=255)
                                                                cTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGGAGGGAGCGCGGAGAGGGGTTTTATTGCCTGACTGAACTGGGGGTTAAGgtt                                                                                   >  1:628963/1‑149 (MQ=255)
                                                                cTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAATTTTTCTTTTTTTTCCCCGAGGAGAGAGGGCGGTGGGGGATTATCTTTCCTGTGTGAACTTGGGATTAAGttt                                                                                   >  1:1075289/1‑149 (MQ=255)
                                                                 ttATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGGAGGGAGCGCGGAGGGGGATTATTTTTGCTGACTGAACTGGCGGTTAAGTTTcc                                                                                 >  2:156950/1‑150 (MQ=255)
                                                                  tataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTcc                                                                                 <  2:842234/149‑1 (MQ=255)
                                                                         tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTTCGCGTAccc                                                                         >  1:1056156/1‑150 (MQ=255)
                                                                         tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCCTGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGGGGTTAAGTTTCCGCGTAccc                                                                         >  1:598765/1‑150 (MQ=255)
                                                                          ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCgg                                                                       <  1:764501/151‑1 (MQ=255)
                                                                               ccTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCTTTTTTTTTTTTTTTGGCATGATAAGGGAGCGCTGAGAGGGATTATCTTTGCTGACTGAAATGGCGCTTAAGATTCCGCGTGACCGGCGttt                                                                  >  1:408143/1‑151 (MQ=255)
                                                                               ccTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCAGAGAGGGGTTTTCTTGCCTGACTGAACTGGCGATTAAGTTTTCGCGTACCCGGCGgtt                                                                  >  2:272447/1‑151 (MQ=255)
                                                                               ccTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTAATGTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGttt                                                                  <  1:946888/151‑1 (MQ=255)
                                                                                  ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGt                                                               <  1:688149/151‑1 (MQ=255)
                                                                                     ccAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGGGAGGGCAGGGGGGGGTTTTTTTGTCTGAATGAACTGGCGGTTTAGTTTTCGCGTACCCCGCGGTTTGtttt                                                            >  2:94227/1‑149 (MQ=255)
                                                                                      cAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTAtt                                                           <  1:606207/151‑1 (MQ=255)
                                                                                       aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTg                                                          <  1:884032/151‑1 (MQ=255)
                                                                                          cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCg                                                        <  1:718902/150‑1 (MQ=255)
                                                                                           aGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGCGCCGGGGGGGATTTTTTTTCCTGGCTGGACTGGCGGTTAAGTTTTCGCGTACCCCGCGGTTTGTTATTGCgg                                                       >  2:151992/1‑150 (MQ=255)
                                                                                           aGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAGCGCAGAGAGGGATTATCTTGGCTGACTGAAATGGCGGTTAAGTTTCCGCGTACCCGGCGTTTTGTTTTTGCgg                                                       >  2:878684/1‑150 (MQ=255)
                                                                                            ggAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgat                                                     <  2:809987/151‑1 (MQ=255)
                                                                                             gAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTGCCATGAGAAGGGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGGTTAAGTTTCCGCGTACCCGGCGTTTTGTTTTTTCgggt                                                     >  1:470181/1‑148 (MQ=255)
                                                                                               gTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCCTGAGAAGTGAGCGCCGAGAGGGGTTTTTTTTCCTGACTGAAATGGCGATTAAGGTTTCGCGGACCCCGCGGTTAGTTAttttgg                                                       >  2:654987/1‑142 (MQ=255)
                                                                                                tCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTCTTTTTCTGCCCTGAGAAAGGAGAGCAGAGAGGGATTATCTTGCCTTACTGAAATGGGGATTTAGTTTTCCCGTAACCGGCGGTTTGATATTGCggg                                                      >  1:455413/1‑145 (MQ=255)
                                                                                                  aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGTCCTGAGAAGTGAGCGCAGAGAGGGGTTATTTTTTCTGACTGAAATGGGGATTTAGTTTTCGCGTACCCGGGGGTTTGTTTTTGCgg                                                       >  2:840565/1‑143 (MQ=255)
                                                                                                  aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgatgat                                               <  1:663776/151‑1 (MQ=255)
                                                                                                  aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgatgat                                               <  2:230606/151‑1 (MQ=255)
                                                                                                   tttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTGCCATGAGAAGGGAGCGCAGAGAGGGATTATCTTTCCTGACTGAACTGGCGGTTTAAGTTTCGCGGACCCGGCGGTTTGTTATTGCGggtgatgat                                               >  1:326521/1‑150 (MQ=255)
                                                                                                      cAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATAtt                                            <  1:622579/150‑1 (MQ=255)
                                                                                                       aGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGTCCTGAGAAGTGAGGGCAGGGAGGGGTTATCTTTCCTGACTGAACTGGCGGTTAAGGTTTCGCGGACCCCGCGGTTAGTTTTTTCGGGTGATGGTAtt                                            >  2:393404/1‑149 (MQ=255)
                                                                                                         gATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGGGAGGGGTTATCTTTCCTGACTGAACTGGCGATTTAGTTTTCGCGTACCCGGCGTTTTGTTATTGCGGGTGATGATAtt                                            >  1:81/1‑147 (MQ=255)
                                                                                                         gATGCCATGTCGCGGATCTCGTTTGCGGCGGCCATTTTTTTTTTTTTTGCCATTGGGCGTGAGCTGAGAGCGGGGATATTTTGCCTTTCCTCACTGGTGTTTAAGTTTTTGCGTACCCCGCGTTTTGTTATTTCGGATGATGTTAttttac                                        >  2:450412/1‑147 (MQ=255)
                                                                                                            gCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCt                                      <  2:407098/150‑1 (MQ=255)
                                                                                                                           tcGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCCTTAGAAGTGGGGGCCGAGGGGGATTTTTTTTTCTGACCTAACCGGGGGTTAAGGTTCCCCGGACCCCGCCTTTTGGTATTTCGGGTGATGATATTGAAGACCGGCTGATTGGtt                         >  2:759992/1‑148 (MQ=255)
                                                                                                                            cGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCa                       <  2:93599/149‑1 (MQ=255)
                                                                                                                             gTTTGCGGCGGTCATTTTTTTTTTTTCTGCCATGAGAAGGGAGGGCAGAGAGGGGTTTTCTTTCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGGGGGTGATGATATTGAAGCTCGGCTGATTGGTTTAtt                     >  2:960448/1‑150 (MQ=255)
                                                                                                                                tGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTc                  <  2:54513/150‑1 (MQ=255)
                                                                                                                                   ggcggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCa              <  1:840599/151‑1 (MQ=255)
                                                                                                                                    gcggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt             >  1:786656/1‑151 (MQ=255)
                                                                                                                                         cATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCg        <  1:515358/151‑1 (MQ=255)
                                                                                                                                              tttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTgg    <  2:1041252/150‑1 (MQ=255)
                                                                                                                                               ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGa   <  2:503028/150‑1 (MQ=255)
                                                                                                                                               ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAc  >  2:749662/1‑151 (MQ=255)
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ATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAC  >  NC_000913/4604199‑4604492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: