Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,252,657	0	C	T	42.9%	61.5 / 22.6	49	L320F (CTT→TTT)	yeiI	putative kinase
Reads supporting (aligned to +/- strand):  ref base C (6/22);  new base T (21/0);  total (27/22)
Fisher's exact test for biased strand distribution p-value = 7.74e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGAT‑ACAGTTTCCGCGAC‑AGC‑GCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCG‑CCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGG  >  NC_000913/2252507‑2252800                                                                                                                                                       |                                                                                                                                                     gtTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTc                                                                                                                                                      <  1:176434/151‑1 (MQ=255)    cAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCtt                                                                                                                                                    <  2:768324/150‑1 (MQ=255)     aGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGa                                                                                                                                                  <  1:855008/151‑1 (MQ=255)            gtttgtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGAT‑a                                                                                                                                           <  1:411139/150‑1 (MQ=255)              ttgtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGTATATAACtttttt                                                                                                                                                    >  1:132857/1‑140 (MQ=255)                  ttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAGCTTTTTTTTAGGAT‑ACAGttt                                                                                                                                     >  2:165525/1‑150 (MQ=255)                      ttGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAACTTTTTTGAAGGGA‑AAAGTTTcc                                                                                                                                   >  1:513203/1‑148 (MQ=255)                       tGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGGTATAGAtttttttttggggaaaattt                                                                                                                                      >  2:275466/1‑131 (MQ=255)                          ccGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGCTATAAATTTCTTTTAGGAT‑AAAGTTTCcgc                                                                                                                                 >  1:20819/1‑146 (MQ=255)                           cGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGGATATAAATTTTTTTAAGGGT‑ACAGTTTTcgc                                                                                                                                 >  2:211744/1‑145 (MQ=255)                               gAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAGCTTTTTTTAAGGAT‑AAAATTTTCGCGAC‑AGC‑Gccc                                                                                                                     >  2:147234/1‑151 (MQ=255)                                aGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATATCTTTTTTTAAGGAT‑ACAGTTTCCCCGAC‑AGC‑Gccc                                                                                                                     >  2:607039/1‑150 (MQ=255)                                   cAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGTCCGGGAGACAGTTTTTTTTGAGGGA‑ACTGTTTTCGCGCC‑AAC‑GCCCGctt                                                                                                                 >  2:60780/1‑151 (MQ=255)                                   cAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGAT‑ACAGTTTCCGCGAC‑AGC‑GCCCGttt                                                                                                                 <  1:741838/151‑1 (MQ=255)                                   cAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGAT‑ACAGTTTCCGCGAC‑AGC‑GCCCGtt                                                                                                                  <  2:217065/150‑1 (MQ=255)                                    aGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGAT‑ACAGTTTCCGCGAC‑AGC‑GCCCGtttt                                                                                                                <  2:697310/151‑1 (MQ=255)                                        tATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGGTATATCTTTTTTTTAAGAT‑ACAGATTCCGCGAC‑AGC‑Gccccctttt                                                                                                               >  2:917738/1‑142 (MQ=255)                                              aGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATATCTTTCTTTTAAGGT‑ACAAGTTCCGCCCC‑CGC‑Gcccccctttcggtggccg                                                                                                      >  1:443252/1‑136 (MQ=255)                                               gCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGAT‑ACAGTTTCCGCGAC‑AGC‑GCCCGTTTTGCGGTAGCCTg                                                                                                     <  1:214490/151‑1 (MQ=255)                                                cGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGGTATAACCTTTTTTTAGGGT‑ACAGTTTTCGCGCC‑AGA‑Gccccctttttgggagcc                                                                                                       >  1:814847/1‑134 (MQ=255)                                                 gAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAACTTTTTTGAAGGGT‑ACAATTTTCGCGAC‑AGC‑GCCCGGTTTTCGGTAGGCt                                                                                                      >  2:452970/1‑148 (MQ=255)                                                       gATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAACTTTTTTGTAGGAT‑ACAGTTTTCGCGGC‑AGC‑GCCCCGTTTTCGGGAGCCTGCGCgg                                                                                                >  2:363457/1‑148 (MQ=255)                                                        aTGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATATGTTTTTTGTAGGAT‑AAAGTTTTCGCGAC‑AAC‑GCCCGGTTTTCGGTGGGCCGCTCGGCact                                                                                            >  1:847008/1‑149 (MQ=255)                                                         tGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGGTATAAATTTTTTTTAGGAG‑AAAATTTTCCCGAC‑AGC‑GCCCGCTTTTGGGGAGCCTGCGCgg                                                                                                >  1:420944/1‑146 (MQ=255)                                                             gaACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGAT‑ACAGTTTCCGCGAC‑AGC‑GCCCGTTTTGCGGTAGCCTGCGCGGCAATTTcg                                                                                        <  1:648436/150‑1 (MQ=255)                                                                 aaTTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCCGGATATTGATTTTTTTTAGGGT‑ACAGATTTTCCGGC‑AGC‑GCCCGTTTTTTGGGAGCCTGCGCGGCGATTTTgcgcg‑c                                                                                  >  2:399041/1‑151 (MQ=255)                                                                    ttttGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATTTAGCTTTTTTTAAGGGT‑AAAGTTTTCGCCGC‑AGC‑GCCCGTTTTTCGGTAGCCTGCGCGGCAATTTTGCCCG‑cc                                                                                 >  2:446844/1‑149 (MQ=255)                                                                     tttGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGAT‑ACAGTTTCCGCGAC‑AGC‑GCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCG‑CCAg                                                                               <  1:784615/150‑1 (MQ=255)                                                                           gACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGTATATAGCTTTTTTTGAGGAT‑ACAGTTTTCGCGGC‑AGC‑GCCCCCTTTTCGGGAGCCCGCGCGGCAAATTTTCCCG‑CCCGCGGCAG‑c                                                                       >  2:594398/1‑151 (MQ=255)                                                                                cgcCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAACTTTTTTTTAGGAT‑ACAATTTCCCCGCC‑AGC‑GCCCCCTTTTCGGTAGCCTGCGCGGCAATTTTGCGCG‑CCCGCGGCAG‑CTTaa                                                                   >  2:867973/1‑150 (MQ=255)                                                                                 gcCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGAT‑ACAGTTTCCGCGAC‑AGC‑GCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCG‑CCAGCGGCAG‑CTTAaac                                                                 <  1:385488/151‑1 (MQ=255)                                                                                    aGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGAT‑ACAGTTTCCGCGAC‑AGC‑GCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCG‑CCAGCGGCAG‑CTTAaacaac                                                              <  2:742784/151‑1 (MQ=255)                                                                                    aGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGGTATAGCTTTCTTTTAGGAT‑AAAGGTTTCGCGAC‑CGC‑GCCCGGTTTTCGGGAGGCTCCGCCGCCATTTTGCGCGCCCCGCGGCGC‑CTTaaaaa                                                                >  1:795311/1‑148 (MQ=255)                                                                                           aCGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGTGTATAGCTTTTTTTTAGGAT‑ACAGGTTTCCCGGC‑AGC‑GCCCGTTTTTCGGGAGCCTGCGCGGGCATTTTGCCCG‑GCCGCGGCAGCCTTAAAAAacccaccc                                                        >  1:400571/1‑147 (MQ=255)                                                                                             gACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAGCTTTTTTTTAGGAT‑AACGGTTTCGCGGC‑AAC‑GCCCGGTTTGCGGTAGCCTGCGCGGCAAATTTTCGCG‑CCCGCGGCCG‑CCTAAAAAaacccacccc                                                      >  1:335351/1‑149 (MQ=255)                                                                                                aGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGAT‑ACAGTTTCCGCGAC‑AGC‑GCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCG‑CCAGCGGCAG‑CTTAAACAACCCTACCCTGTCt                                                  <  2:337433/151‑1 (MQ=255)                                                                                                 gTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGAT‑ACAGTTTCCGCGAC‑AGC‑GCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCG‑CCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTg                                                 <  1:381028/151‑1 (MQ=255)                                                                                                  tAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTTGGATATATCTTTTTTTGAGGATAAAAGTTTTC‑CGAC‑CGC‑GCCCGGTTTTTGGTTGCCTGCGGGGCACTTTTGCGCG‑CCCGCGGGAG‑CCTAAAAAaccctcccc                                                       >  1:756497/1‑144 (MQ=255)                                                                                                    gACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGATTTAAGTTTTTTTGAGGAT‑AAAAGTTTCGCGGCAAGC‑CCCGGTTTTG‑GGTAGCCTGCGCCGGAATTTTTCGCC‑CCCCCGGCAGCCTTAAAAAacccaccccctcctcc                                                >  1:194196/1‑138 (MQ=255)                                                                                                    gACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGAT‑ACAGTTTCCGCGAC‑AGC‑GCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCG‑CCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCg                                              <  2:513231/151‑1 (MQ=255)                                                                                                     aCAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCGCGGTATATAGCTTTTTTTTAGGAT‑AAAGGTTTCGCGAC‑GGCGGCCCGTTTT‑CGGGAGCCTGCGCGGCGCTTTTTCCCG‑CCACCGGGGG‑CGTAAAAAaaccccccccccctgcc                                               >  1:620470/1‑136 (MQ=255)                                                                                                       aGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGAT‑ACAGTTTCCGCGAC‑AGC‑GCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCG‑CCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATa                                           <  2:380367/151‑1 (MQ=255)                                                                                                                    aCGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGAT‑ACAGTTTCCGCGAC‑AGC‑GCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCG‑CCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCAtt                              <  1:448348/151‑1 (MQ=255)                                                                                                                       aTGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGAT‑ACAGTTTCCGCGAC‑AGC‑GCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCG‑CCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGt                           <  2:555796/151‑1 (MQ=255)                                                                                                                       aTGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGAT‑ACAGTTTCCGCGAC‑AGC‑GCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCG‑CCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGt                           <  2:795350/151‑1 (MQ=255)                                                                                                                          gTTTTACGGCGGGCCTGGTATATAGCTTTCTTGAAGGAT‑ACAGTTTCCGCGAC‑AGC‑GCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCG‑CCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGcc                        <  2:335371/151‑1 (MQ=255)                                                                                                                               aTGGCGGGCCTGGTATATAGCTTTCTTGAAGGAT‑ACAGTTTCCGCGAC‑AGC‑GCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCG‑CCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATg                    <  2:633061/150‑1 (MQ=255)                                                                                                                                 ggCGGGCCTGGTATATAGCTTTCTTGAAGGAT‑ACAGTTTCCGCGAC‑AGC‑GCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCG‑CCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATgg                   <  1:414548/149‑1 (MQ=255)                                                                                                                                 ggCGGGCCTGGCATATAGCTTTCTTGAAGGAT‑ACAGATTCCGCGAC‑AGC‑GCCCGCTTTGCGGTAGCCTGCGCGGCAATTTCGCGCG‑CCAGCGGCAG‑CTTAAACAACCCGACCCCGTCTGCCGATAACGCGCCTTCATTAGTGCCAATg                    >  2:887484/1‑148 (MQ=255)                                                                                                                                      gCCTGGTATATAGCTTTCTTGAAGGAT‑ACAGTTTCCGCGAC‑AGC‑GCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCG‑CCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACa            <  2:205206/151‑1 (MQ=255)                                                                                                                                                taGCTTTCTTGAAGGAT‑ACAGTTTCCGCGAC‑AGC‑GCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCG‑CCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTACATTAGTGCCAATGGTGTAACAATGTTGCCgg  >  1:33733/1‑151 (MQ=255)                                                                                                                                                 aGCTTTCTTGAAGGAT‑ACAGTTTCCGCGAC‑AGC‑GCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCG‑CCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCgg  >  2:696201/1‑150 (MQ=255)                                                                                                                                                       |                                                                                                                                                     GTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGAT‑ACAGTTTCCGCGAC‑AGC‑GCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCG‑CCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGG  >  NC_000913/2252507‑2252800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑