Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,337,723 | 0 | A | C | 43.3% | 91.5 / 22.5 | 60 | G566G (GGT→GGG) | gyrA | DNA gyrase (type II topoisomerase), subunit A |
Reads supporting (aligned to +/- strand): ref base A (28/6); new base C (0/26); total (28/32) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.45e-11 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGATTTCAGTACG > NC_000913/2337576‑2337869 | gcgcgcgcCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCt < 2:252234/151‑1 (MQ=255) cgcgcgcCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCtt < 2:459027/151‑1 (MQ=255) gAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCacg > 2:779953/1‑151 (MQ=255) tCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTgcg > 2:247128/1‑151 (MQ=255) aCTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAAAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGt > 2:806569/1‑150 (MQ=255) aTAAACTTTCATCGAATAGACGCGACCCCGGCTGGAGAAGCAAAGAATAGGGTCGGGAGTGTTCCCCACCAGCAGTCGGTCAATAAAGTCTTCTTTTTTAATCCGTGCGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTAt < 2:498250/148‑1 (MQ=255) tAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTc > 1:687556/1‑149 (MQ=255) aaaCTTTCATCGAATAGACGCGCCCACGGCTGGAGAAGCACAAAATATGGTCGTGGTTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTCCTTTTCCCGCCACGACGCTGCGCTTCGTATTCAg < 2:633221/150‑1 (MQ=255) aaaCTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTc > 1:679259/1‑148 (MQ=255) aaCTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGAGCGGCAGATTTACGTTTCCCGCCACGACGCTGCGCTTTGTATTCAGa > 1:523294/1‑150 (MQ=255) ttCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCg > 2:726286/1‑151 (MQ=255) ttCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCg > 2:506479/1‑151 (MQ=255) aaTAGCCGCGACCACGGCTGGAGAAGCACAGAATATGGTCGGGAGTTTTCGCCCCCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATCCGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTg < 2:605812/148‑1 (MQ=255) aaTAGACCCGCCCCCGGCGGGAGAAGAACAGAATATGGTCGTGGGTGTCCCCCCCCAGCAGTCGGTCGATAAAGTCTTCTTTTTAAATACGTGGGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATa < 1:322302/151‑1 (MQ=255) gACGCGCCCACGGCGGGAGAAGCACAAAATATGGTCGTGTGTGTCCCCCACAAGCAGTCGTTCAAAAAAGTCTTCTTTTTTAATACTTGCGGAAGATTTCCTTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACtt < 1:903176/150‑1 (MQ=255) cgcgCCCCCGCCTGGAAAAGCACAGAATATGGTCGTGATTGTCCCCCCCCAGCAGTCGGTCGATAAAGTCTTCTTTTTTAATCCGTGCGGCAGATTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTaa < 2:68276/150‑1 (MQ=255) ccACGGCTGGAGAACCACAGAATATGGTCGTGAGTGTCCGCCCCCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACg < 2:372198/147‑1 (MQ=255) cGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCt > 2:572527/1‑151 (MQ=255) tGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTg > 1:292623/1‑151 (MQ=255) ggAGAAGCACAGAATTTGGTCGTGAGTGTTCCCCCCCAGCAGTCGGTCAAAAAATTCTTCTTTTTTAATCCGTGCGGAAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTgg < 1:764776/148‑1 (MQ=255) ggAAAACCACAAAATATGGTCGTGATTTTTCCCCCCCACCTGTCGTTCAAAAAATTTTTCTTTTTTAATCCGTGCGGAAATTTTCCTTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTga < 1:592566/151‑1 (MQ=255) gagaAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTg > 2:768812/1‑149 (MQ=255) agaAGCACAGAATATGGTCGTGAGTTTTCCCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTgaga < 2:225658/151‑1 (MQ=255) aaGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTTCGGCAGATTTACCTTTCCCGCCACGAAGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACCTAGCCCTGGTgcg > 1:563807/1‑146 (MQ=255) aaGCACAGAATAGGGTCGTGAGTGTCCCCCACCAGCAGTCGGTCGATAAAGTCTTCTTTTTTAATACGTGCGGAAGATTTCCTTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTgagaga < 1:173565/151‑1 (MQ=255) aaGCAAAAAATATGGTGGTGAGTGTCCGCCACCAGCAGTGGGTCGAAAATGTCTTTTTCTTTAATGCGGGGGGAGGATTTCCTTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTgaga < 1:779330/149‑1 (MQ=255) aaGCAAAAAATAGGGTGGTGAGTGTTCCCCCCCAGCAGTCGGTCGATAAAGTCTTCTTTTTTAATGCGTGGGACATATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTgagag < 1:37665/150‑1 (MQ=255) aaattatGGTCTTGGGTGTCCCCCCCCAGGAGTCGCTCAATATCTTTTTCTTCTTTATTCCGTCCGTCATTTTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCa < 1:506500/147‑1 (MQ=255) gTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCt > 2:426922/1‑151 (MQ=255) ttCGCCCCCAGCAGTCGGTCAATAAAGTTTTCTTTTTAAATCCGTGCGGCAGTTTCCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTgg < 1:886286/150‑1 (MQ=255) ttCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTggg > 1:376024/1‑151 (MQ=255) tccGCCCCCAGCAGTCGGTCGATAAATTCTTCTTCTTTAATACGGGCGCAAGATTTACCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCt < 1:534124/146‑1 (MQ=255) tCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGt > 2:748710/1‑151 (MQ=255) gCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTg > 2:406781/1‑150 (MQ=255) ccccccaGCAGCCGGTCAATAAGTTCTTCTTTTTTAATCCGTGGGGCAGTTTTCCTTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTg < 1:782289/146‑1 (MQ=255) ccaccaGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGAt > 2:150583/1‑151 (MQ=255) caccaGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATc > 2:169513/1‑151 (MQ=255) ccaGCAGTCGGTCGATAAATTCTTCTTTTTTAATACGTGCGGCATTTTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCa < 1:491992/150‑1 (MQ=255) cggcagTCGTTCGAAAAGGTCTTCTTCTTTAATACGTGCGGCATATTTCCTTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCa < 1:680539/147‑1 (MQ=255) cagcagTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATc > 2:708634/1‑148 (MQ=255) agTCGTTCGATAAAGTCTTCTTCTTTAATCCGTGCGGCAGTTTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGAt < 2:857144/148‑1 (MQ=255) agTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCtt > 1:887618/1‑151 (MQ=255) agTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCtt > 2:363400/1‑151 (MQ=255) tcggtcgAAAAAGTCTTCTTCTTAAATCCGTGCGGCAGATTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTc < 1:748671/150‑1 (MQ=255) cgatggagtaggttttcttcttTAAAGCGTCGGTGAGTTTTACGTTGCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCTTCACGACCACATCTTCCTGGGTGATCAGATc < 1:186092/132‑1 (MQ=255) ggtcgATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCa < 1:308339/150‑1 (MQ=255) ggtcgATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTcc > 2:220347/1‑149 (MQ=255) ggtcgATAAAGTCTTCTTCTTTAATACGGGCGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCa < 2:155074/150‑1 (MQ=255) ggtcgATAAAGTCTTCTTCTTTAATACGGGCGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCa < 1:708595/150‑1 (MQ=255) gtcaATAAAGTCTTTTTTTTTAATACGGGCGGCAGATTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTcc < 1:806529/148‑1 (MQ=255) tcttcttTAATCCGTGGGGAAGTTTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTgcg < 2:11400/150‑1 (MQ=255) tgtttATTTCGTGGGCCATATTTCCCTTTTCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGAGGTTTg < 1:350289/143‑1 (MQ=255) tcttTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGt > 1:904354/1‑151 (MQ=255) aTACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCgg > 2:50795/1‑151 (MQ=255) aCGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTg > 2:153168/1‑151 (MQ=255) tGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGAtt > 2:777112/1‑151 (MQ=255) ggggCAGATTTCCTTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGAttt < 2:246386/149‑1 (MQ=255) aGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGATTTCAGt > 2:328476/1‑150 (MQ=255) aTTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGATTTCAGTacg > 2:802891/1‑151 (MQ=255) tttCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGATTTCAGt < 2:215224/147‑1 (MQ=255) | GCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGATTTCAGTACG > NC_000913/2337576‑2337869 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |