Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,434,882 | 0 | T | G | 44.1% | 81.9 / 27.8 | 60 | Y252S (TAC→TCC) | truA | tRNA pseudouridine(38‑40) synthase |
Reads supporting (aligned to +/- strand): ref base T (4/29); new base G (26/0); total (31/29) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.84e-13 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCAT > NC_000913/2434736‑2435030 | gTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGAACCTAACGGTCAGGGGAATc > 1:500919/1‑151 (MQ=255) gTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATc < 1:275473/151‑1 (MQ=255) ccATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGATCATACCGGTCCGGGGAATcc > 1:325927/1‑150 (MQ=255) cATATTTGCCTATGCCGTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCa < 1:824137/150‑1 (MQ=255) atatTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGATCATACCGGTCAGGGGAATCCAcc > 1:670470/1‑151 (MQ=255) tatTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGATCATACCGGTCAGGGGAATCCAcc > 2:185526/1‑150 (MQ=255) tatTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAAATCATAACCGGCAGGGGAATACAccc > 1:388047/1‑150 (MQ=255) atTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACcg < 1:28123/150‑1 (MQ=255) tGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGATCATACCGGGCAGGGGAATCCAcccccac > 1:922709/1‑146 (MQ=255) tGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCACCAGAAATAGCTGGCCCATTGGCGG‑GTTTTGAAGAACATACCCGTCCGGGAAAACCACCGCGa > 2:385754/1‑150 (MQ=255) ccTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGGAGATCATAACCGGCAGGGGAATCCACCCCGAcc > 1:569148/1‑150 (MQ=255) gCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATa < 1:132518/150‑1 (MQ=255) ccTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACa < 1:64269/151‑1 (MQ=255) ttGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCCGGCAGGGGAATCCACCGCGACCAGATACa > 2:892250/1‑149 (MQ=255) tACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGGAATCCACCCCGACCAGATAAAAccc > 2:878909/1‑150 (MQ=255) aCTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCtt < 1:835115/151‑1 (MQ=255) aCTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCtt < 2:798478/151‑1 (MQ=255) cTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGATCATACCGGGCCGGGGAATCCAACCCGACCCGATAAAGacc > 1:449588/1‑148 (MQ=255) cGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGGAATCCACCCCGCCCAGATACAACCCTTcc > 1:148343/1‑150 (MQ=255) aTCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCGCttt > 2:477190/1‑151 (MQ=255) tCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCAGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑ATTTTGAAAAGAATACCGGAGAGGGGAATCCCCCCCGGCCAGAAACAGGCCTTCACCtttt > 1:829745/1‑151 (MQ=255) cTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCCACCCGATACAACCCTTCCCCtttt > 2:565855/1‑150 (MQ=255) tttCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGATCATACCGGGCAGGGGAAACCACCCCGACCACATACAGCCCCTCCCCtttt > 2:567301/1‑149 (MQ=255) cAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCTGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCg < 1:506543/150‑1 (MQ=255) gTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCg < 1:299638/151‑1 (MQ=255) gCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGtt < 1:323863/149‑1 (MQ=255) aCAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGAGCATACAGGGTAAGGGAATCCACCGGGAACAGATAAAGCCCCTCCGCTTTTTGCATTGCCGCCTCCACCGGTCTGt > 2:455458/1‑150 (MQ=255) aCAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTg < 2:486800/149‑1 (MQ=255) aGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTTCCGTTGCTGCCCCCCGCGGTCTGTcc > 1:361176/1‑150 (MQ=255) aGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCt < 2:422051/151‑1 (MQ=255) ccTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATAACGGTCAGGGGAATCCCCCGCGACCAGATACAACCCTTCCCCTTTTTCCGTTGCTGCCCCCCGCGTTCTGTCCttt > 2:587207/1‑151 (MQ=255) ccTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGAACATACCGGTCAGGGGAATCCACCGCGACCAGATAAAGCCCCTCCGCTTTTTCCGTTGCTGCCGCCAGCGTTCTGTCCttt > 2:636158/1‑151 (MQ=255) cTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCGCCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCttt < 1:410184/150‑1 (MQ=255) gAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTg < 2:112526/149‑1 (MQ=255) aaaTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTgccg < 1:915728/151‑1 (MQ=255) gTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCa < 2:110691/150‑1 (MQ=255) cGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGAAGATAAAAGGCGTGGGAAACCCCCCCGCCCAAATACAAGCCTTCCGCTTTTTCCGTTTGTGGCCCCACCGCTCTTTGCTTTgccgcc > 1:194294/1‑145 (MQ=255) cGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAAc < 1:652554/151‑1 (MQ=255) cATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGTAGATTCTACCCGTCAAGGGAAAACACCGCCACCCGAAACAGCCCTTCCCCTTTTGTCGGTGCTGCCGCCCGCGGTCTTTCCTTTTCCCCCCGCAAct > 1:186427/1‑148 (MQ=255) ttattCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGATCATACCCGGCAGGGGAATCCACCCCGACCAGATACAGCCCCTCCCCTTTTGCCGTTGCTGCCCCCAGCGGTCTGTCCTTTTCCGCCCGCAActc > 2:879312/1‑147 (MQ=255) tctcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGGAGATCATACCCGGCAGGGTAAACCACCGCGACCCGACACAACCCTTCCGCCTTTTCCGCTGCTGCCGCCCGCGTTCTTTCCTTTGCCGCCCGGCACCCAGCTATcc > 1:574493/1‑151 (MQ=255) tctcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATc < 2:14262/150‑1 (MQ=255) tAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGCCCTTTGCCGCCAGCAACTCTGCTATCCa < 1:385731/146‑1 (MQ=255) gTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTcc < 2:914609/151‑1 (MQ=255) cGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGGAGATCATACCGGTCAGGGGAATCCCCCCCGACCAGATACAGCCCCTCCGCTTTTTCCGTTGCTGCCGCCAGCGGTCTGTCCTTTTCCCCCAGCAACTCTGCTATCCAGCTCTCCgg > 2:740773/1‑150 (MQ=255) gCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGGAT‑ATACCGGGCAGGGGAATTCACCGCGACCAGAAACAACCCCTCCGCTTTTTCCGTTGCTGCCGCCAACGTTTTGTTCTTTTCCGCCAGCCACTCCGCTTTCCCACTCTCCGGc > 2:60399/1‑150 (MQ=255) ccAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATAACCGTTAGGGGAATCCACCCCGACCAGATACAGACCTTTCGCTTTTTCCGGTGCTGCCGCCCCCGTTCTTTCCTTTTCCCCCCGCCACCCCTCTATCCCGCTCTcc > 2:784958/1‑146 (MQ=255) aGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCAAACCAGGCAGGGGAAACCACCCCCACCAGAAACAGCCCCTCCCCTTTTTCCGTTGCTGCCGCCCGCGTTCTGTCCTTTTCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTgg > 1:787167/1‑150 (MQ=255) gAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGtt < 2:26062/151‑1 (MQ=255) aaaTAGCGGGCCCATTGGCGG‑TTTTGGGAGATCATACCGGGCAGGGGAATTCACCCCGACCCGATACAGCCCTTCCGCCTTTTCCCGTGCTGCCCCCCGCGTTCTGTTCTTTGCCGCCAGCAACTCTGCTATCCCGCCCTCCCGCTg > 1:479199/1‑147 (MQ=255) aTAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTtgtg < 1:740082/151‑1 (MQ=255) ggCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCa < 1:286562/151‑1 (MQ=255) gg‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCa < 2:602539/151‑1 (MQ=255) g‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATc > 1:622402/1‑149 (MQ=255) tttGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGc > 2:166135/1‑151 (MQ=255) aaGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCg < 1:772267/151‑1 (MQ=255) aGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGa < 1:273515/151‑1 (MQ=255) cATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAat < 2:844153/151‑1 (MQ=255) aCCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATAtt < 1:932734/151‑1 (MQ=255) ggTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCat < 2:923811/151‑1 (MQ=255) | GTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCAT > NC_000913/2434736‑2435030 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |