Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,630,340	0	A	C	43.6%	83.0 / 16.2	55	G61G (GGT→GGG)	ettA	energy‑dependent translational throttle A
Reads supporting (aligned to +/- strand):  ref base A (24/7);  new base C (0/24);  total (24/31)
Fisher's exact test for biased strand distribution p-value = 1.11e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCG  >  NC_000913/4630193‑4630490                                                                                                                                                    |                                                                                                                                                       cAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCt                                                                                                                                                     <  1:958610/151‑1 (MQ=255)      cATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGAt                                                                                                                                                >  2:101675/1‑151 (MQ=255)      cATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGAt                                                                                                                                                <  1:108303/151‑1 (MQ=255)         acacTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGt                                                                                                                                              <  1:919926/150‑1 (MQ=255)             ttCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCttt                                                                                                                                          >  2:527154/1‑150 (MQ=255)                  ccAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAAt                                                                                                                                    >  2:471247/1‑151 (MQ=255)                                gCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATGTCAGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATg                                                                                                                       >  1:358900/1‑150 (MQ=255)                                   ttAACCACTTCGAAACCCGCTTCTTCAAGGGCCCCCCGCACGGTGTGTTCGGGGTTCAGCGGCGTTTCCTGCGGCAGATAACCATTCTTGATGTCGGGCGGCGGACGCGCTTCCCCTTCGTTGTCTTTTTCAATGCCCGCCATAATgcg                                                                                                                     <  1:267351/149‑1 (MQ=255)                                       ccACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCgcagc                                                                                                                 >  2:837715/1‑149 (MQ=255)                                        cACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAgg                                                                                                              >  1:550905/1‑151 (MQ=255)                                            tCTGAACCCGCTTCTTCAATGGACTCCCGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATTTCTGGCTGCGGACGCGCTTCCCCTTCGATGTTTTTATCAATGCCCGCCATAATGCGCAGCAGGGTg                                                                                                           <  2:589358/150‑1 (MQ=255)                                             ctaaacccGCTTCTTAAATGACCTCCCGCACGTTGTTTTCGGGGTTCAGCTGCGGTTCCGGCGGCAAATAACCAATTTTGATGTCTGGCTGCGGACGCGCTTCCCCTTCGTTTTTTTTATCAATGCCCGCCATAATGCGCAGCAGGGt                                                                                                            <  1:313334/142‑1 (MQ=255)                                                aacccctttcttcAATGGGCCCCCGCAGGGTGTGTCCCGGTTTCAGCGGGGGTCCCTGCGGCAGATAACCAGTTTTGATGTTTGGCTGGGGACGCGCTTCCCCTTCGATTTCTTTATAATTGCCCGCCATAATGCGCAGCAGGGTGGa                                                                                                         <  2:807466/141‑1 (MQ=255)                                                 aaCCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACtt                                                                                                      >  1:915450/1‑150 (MQ=255)                                                     gCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCcg                                                                                                 >  1:471346/1‑151 (MQ=255)                                                       ttcttcAATGGACTCACGCAGGGTGTGTTCCGGTTTCAGCTGCGGTTCCTGCGGCAGATAACCATTCTTGATGTCTGGCTGCGGCCGCGCTTCCCCTTCGATTTTTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTAccc                                                                                                  <  2:761269/148‑1 (MQ=255)                                                       ttcttcAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCcgcg                                                                                               >  2:318445/1‑151 (MQ=255)                                                       ttcttcAAGGGCCTCACGCACGGTGTGTTCCGGTTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCCCCTTCGATTTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCcg                                                                                                 <  2:923455/149‑1 (MQ=255)                                                            cAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCAtt                                                                                          >  1:876427/1‑151 (MQ=255)                                                            cAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCAtt                                                                                          >  1:86614/1‑151 (MQ=255)                                                             aagggACTCACGCCCGGTGTGTTCCGGGTCCAGCTGCGGTTCCTGCGGCAAATACCCAATTTTGATGTCTGGCTGCGGCCGCGCTTCCCCTTCGATTTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCa                                                                                            <  2:505316/145‑1 (MQ=255)                                                                ggACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTc                                                                                         >  2:497639/1‑148 (MQ=255)                                                                      acgcacgGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAgg                                                                                >  2:954220/1‑151 (MQ=255)                                                                       cgcacgGTGTGTCCGGGGTTCAGCTGCGGTTCCTGCGGCAGATAACCAATTTTGATGTCTGGCTGCGGACGCGCTTCCCCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGAcc                                                                                    <  2:285592/146‑1 (MQ=255)                                                                        gcacgGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGac                                                                              >  2:813899/1‑151 (MQ=255)                                                                            gttgtgtTCCGGGTCCAGCGGCGGTTCCTGCGACAAATACCCATTCTTGATGTCTGGCTGCGGACGCGCTTCCCCTTCGATTTCTTTACCAACGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGaca                                                                             <  1:317083/146‑1 (MQ=255)                                                                              tgtgtTCCGGGTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAAt                                                                        >  2:677272/1‑151 (MQ=255)                                                                                       ggTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGcccc                                                               >  2:152161/1‑151 (MQ=255)                                                                                       ggTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGcccc                                                               >  1:514131/1‑151 (MQ=255)                                                                                         ttCAGCTGCGTTTCCTGCGGCAGATAACCAATTTTGATGTCTGGCTGCGGCCGCGCTTCCCCTTCGATGTTTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCa                                                              <  1:669810/150‑1 (MQ=255)                                                                                         ttCAGCTGCGGTTCCTGCGGCAGATAACAAATTTTGATGTCTGGCTGCGGACGCGCTTCCCCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCa                                                              <  2:575001/150‑1 (MQ=255)                                                                                                gCGTTTCCTGCGGCAGATAACCAATTTTGATGTCTGGCTGCGGCGGCGCTTCCCTTTCGATTTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGgaa                                                         <  1:300533/148‑1 (MQ=255)                                                                                                  gttcccTGCGGCAGATAACCATTCTTGAGGGCTGGCCGGGGCCGCCTTTCCCCTTCGAGTTTTTTACAAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGaaa                                                     <  1:315077/146‑1 (MQ=255)                                                                                                    ttCCTGCGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTc                                                  >  1:152778/1‑151 (MQ=255)                                                                                                        tGCGGCAGATAACCAATCTTGATGTCTGGCTGCGGATGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCag                                                >  2:82356/1‑149 (MQ=255)                                                                                                           ggCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCagag                                              >  1:288599/1‑148 (MQ=255)                                                                                                            gCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCagag                                              >  1:474486/1‑147 (MQ=255)                                                                                                              aaaTACCCATTTTTGTTGTCTGGCTGCGGACGCGCTTCCCCTTCGATTTCTTTATCAAGGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATg                                         <  1:919029/148‑1 (MQ=255)                                                                                                              aGATACCCATTCTTGATGTCTGGCTGCGGCCGCGCTTCCCCTTGGATGTTTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATg                                         <  2:650907/150‑1 (MQ=255)                                                                                                                 tAACAACTCTTAATGTTTGGCGGCGAGTGCGCTTCACTTTCGATTTTTTTAACAATACCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGttt                                      <  2:356258/150‑1 (MQ=255)                                                                                                                          ttgttgTGTGGCTGCGTCCGGGGTTCACGTTGGAGTTTTTAATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAat                              <  1:143956/149‑1 (MQ=255)                                                                                                                          ttGATGTCTGGCGGCGGACGCGCTTCCCCTTCGATGTCTTTACCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAata                             <  2:76675/150‑1 (MQ=255)                                                                                                                           tGATGTCTGGCTGCGGACGCGCTTCCCCTTCGTTTTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAatat                            <  1:300880/150‑1 (MQ=255)                                                                                                                            gATGTCGGGCTGCGGACGCGTTTCCCCTTCGATTTTTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATg                           <  1:585134/150‑1 (MQ=255)                                                                                                                              tGTCTGGCTGCGGACGCGTTTCCCCTTCGATTTTTTTATAAATCCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATg                           <  2:508363/148‑1 (MQ=255)                                                                                                                                tCTGGCTGCGGCCGCGCTTCCCCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGt                       <  2:823084/150‑1 (MQ=255)                                                                                                                                tCTGGCTGCGGACGCGTTTCCCTTTAGTTGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAatat                            <  2:952121/145‑1 (MQ=255)                                                                                                                                         ggccgcgCTTCACCTTCATTGTCTTTAAAAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTcggcg                <  1:508056/145‑1 (MQ=255)                                                                                                                                         ggACGCGTTTCCCTTTGGTTTTTTTTATCAATCCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTcggc                 <  1:167701/147‑1 (MQ=255)                                                                                                                                          gACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGaac            >  2:496264/1‑151 (MQ=255)                                                                                                                                              cgcTTCCCCTTCGATGTCTTTATAAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGaacaa          <  2:251478/149‑1 (MQ=255)                                                                                                                                              cgcTTCACCTTCGATGTCTTTATAAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGaacaa          <  2:65323/149‑1 (MQ=255)                                                                                                                                                cTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACttt      >  2:479524/1‑151 (MQ=255)                                                                                                                                                cTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACttt      >  1:124461/1‑151 (MQ=255)                                                                                                                                                 ttCCCCTTCGTTTTTTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACttt      <  2:65659/150‑1 (MQ=255)                                                                                                                                                    aCCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCg  >  1:68554/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                       CAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCG  >  NC_000913/4630193‑4630490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑