| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 827,507 | 0 | T | G | 56.4% | 29.4 / 50.0 | 39 | D492A (GAC→GCC) | ybhF | putative ABC transporter ATPase |
| Reads supporting (aligned to +/- strand): ref base T (3/14); new base G (22/0); total (25/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.51e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.91e-05 | |||||||||||
TGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTG‑TTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTT > NC_000913/827364‑827631 | tGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGCGAGGGGGTCAACgt > 2:325742/1‑149 (MQ=255) ggCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGcc < 2:175974/150‑1 (MQ=255) gCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGTGaaacgcgg > 1:741794/1‑145 (MQ=255) tAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGt < 1:757373/150‑1 (MQ=255) aaTTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCg < 2:4632/151‑1 (MQ=255) aTTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGTGGGGGGCAACGCCCGAAGGCgg > 1:592303/1‑151 (MQ=255) tttCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGGAggagg > 1:203764/1‑146 (MQ=255) cgcgGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGAAGTCgggt > 2:79871/1‑145 (MQ=255) gcgGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTtcgtcg < 2:573067/151‑1 (MQ=255) cgGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGTCACGCCGGGAAGCGGgtcgtc > 1:509859/1‑149 (MQ=255) gTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGGGGGGGGCAACGCCCGAAGTCGGGtcgt > 2:331309/1‑146 (MQ=255) cacCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGGCAACGCCCGAAGGCGGTTTGGCGAGgaa > 1:709378/1‑150 (MQ=255) ccAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGGAGACGGGTTGTCGAGgaa > 2:688915/1‑148 (MQ=255) aGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGGCAACGCCGGGAGTCGGGTCGTCGAGAAAAAGa > 1:736688/1‑150 (MQ=255) aGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGGCAAAGCCGGAAGGCGGGTCGTCGAGGAACa > 1:727573/1‑148 (MQ=255) gCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGAGCAGCCAAAATTCACGGCGGGAGAGGGGGGCAACGCCGCGAGACGGTTCGCCGCGAAACAg > 2:656690/1‑147 (MQ=255) gTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCa < 1:5527/151‑1 (MQ=255) tCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGGCGGTTCGTCGAGAAACAGAAAGTCCGGTTCAt > 2:343536/1‑151 (MQ=255) ccGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCCGAAGTCGGGTTGTCGAGAAAAAGAATGTCCGGGTCCTGCATCAg > 1:742759/1‑148 (MQ=255) ccGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGGCGGGTCGTCGTGAAAAAAAATGTCCGGGTCATGGCTCAGc > 1:743821/1‑149 (MQ=255) aTCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGgcc < 2:942061/151‑1 (MQ=255) tCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGGAAGCGGTTCGTCGAGAAAAAGAAAGTCCGGTTCATGCATCAGCGAACAAgcc > 2:369353/1‑150 (MQ=255) aGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGgccagcgccag < 1:465590/151‑1 (MQ=255) ggtgACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTg < 1:300448/150‑1 (MQ=255) tgACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGGCAACGCCGGAAGGCGGGTCGTCGAGAAACAGAAAGTCCGGGTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTG‑ttt > 1:528637/1‑151 (MQ=255) aTCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTG‑TTTAAAAc < 2:815114/151‑1 (MQ=255) cACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGGAGGCGGTTCGTCGAGAAAAAGAATGTCCGGGTCATGCCTCCGCGAACAGGCCCGCGCCCGCCGCCG‑TTTtaaaac > 1:530050/1‑148 (MQ=255) cACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGAAGGCGGTTCGTCGAGAAACAGAATGTCCGGTTCATTCAACAGCGAACAGGCCAACGCCCGCCGCCG‑TTTTAAAcc > 2:142976/1‑150 (MQ=255) ccGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTG‑TTTAAACCCTa < 1:770295/150‑1 (MQ=255) gTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTG‑TTTAAAACCTAATg < 1:645698/151‑1 (MQ=255) aCGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTG‑TTTAAAACCTAATGGCa < 2:239342/151‑1 (MQ=255) aCGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGGAATCGGGTCGTCGAGAAACAGAAAGTCCGGGTCATGCATCAGCGAAAAAGCCAGCGCCAGCCCCCG‑GTTTAAACCTAATgg > 2:411212/1‑149 (MQ=255) aCCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTG‑TTTAAAACCTAATGGCAGTTCATCGGTg < 1:303215/150‑1 (MQ=255) cATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGAAGTCGGGTCGTCGAGAAACAGAATGTTCGGGTCATGCATCAACCAAAAGGCCAGCGCCAGCCGCTG‑TTTAAAAACTAATGGCAGGTCATCGGTgg > 2:729220/1‑149 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGGCGGTTCGTCGAGAAACAGAATGTCCGGGTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCCG‑TTTTAAACCCAAAGGCAGTTCATCGGGGGCg > 1:631035/1‑150 (MQ=255) tgCAGCCAAAATTCACGGCGGGAGAGGGGGGGAACGGCGGAAGTCGGGTCGGCGAGAAACAGAATGTCCGGGTCATGCATGAACGGACAAGCCAGGGACAACCGCTG‑TTTAAAAACCAATAGCAGGTCCACGGGGGCgggggggggggta > 2:825910/1‑145 (MQ=255) cAGCCAAAATTCACGGCGGGTGAGGGGGGCCACGCCGGGAGACGGGTCGTCGAGAAAAAGAATGTCCGGGTCCTGCATCAGCGAACAGGCCAACGCCAGCCGCCG‑TTTTAAACCTAATTGCAGGTTATCGGGGGGGGGGGGGGGGGTACtt > 1:489206/1‑151 (MQ=255) gCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTG‑TTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATTCtttt < 1:666501/151‑1 (MQ=255) ccAAAATTCACGGCGGGGGAGGGGGGGAAAGCCCGAAATCGGTTCGTCGAGAAAAAGAAAGTCCGGGTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCCGTTTTAAAACCAAAT‑GCAGGTCATCGGGGGGGGGGGGGGGGGTACtttt > 1:935032/1‑150 (MQ=255) | TGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTG‑TTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTT > NC_000913/827364‑827631 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |