Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,823,799	G→T	R467L (CGC→CTC)	spoT →	bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,823,799	0	G	T	100.0%	311.2 / NA	78	R467L (CGC→CTC)	spoT	bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (34/44);  total (34/44)

CATGCCTGCGTGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAA  >  NC_000913/3823654‑3823946                                                                                                                                                  |                                                                                                                                                    cATGCCTGCGTGGGCGCCCGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGcc                                                                                                                                                 <  1:904965/150‑1 (MQ=255)   tGCCTGCGTGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCaa                                                                                                                                               >  1:75352/1‑150 (MQ=255)      cTGCGTGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAAtt                                                                                                                                           >  2:543495/1‑151 (MQ=255)        gCGTGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTc                                                                                                                                          <  1:819643/150‑1 (MQ=255)          gTGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGt                                                                                                                                        >  1:884774/1‑150 (MQ=255)          gTGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTc                                                                                                                                       <  1:898895/151‑1 (MQ=255)            gggCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTc                                                                                                                                       >  1:805253/1‑149 (MQ=255)               cgcACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTg                                                                                                                                  >  2:97444/1‑151 (MQ=255)               cgcACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAACGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCACTTg                                                                                                                                  >  1:358015/1‑151 (MQ=255)                gcACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGc                                                                                                                                 <  2:23638/151‑1 (MQ=255)                 cACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGc                                                                                                                                 <  1:609749/150‑1 (MQ=255)                 cACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCt                                                                                                                                >  1:581091/1‑151 (MQ=255)                   cgcgTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTg                                                                                                                               >  2:905043/1‑150 (MQ=255)                      gTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGaaaa                                                                                                                           <  2:887589/151‑1 (MQ=255)                        tGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGaaaaa                                                                                                                          <  1:17750/150‑1 (MQ=255)                          aCCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCt                                                                                                                       <  1:860154/151‑1 (MQ=255)                            cGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTc                                                                                                                      >  2:266982/1‑150 (MQ=255)                             gCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCaa                                                                                                                    <  2:744205/151‑1 (MQ=255)                             gCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCCTGGCTGAACTTTGCCGTTCGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCa                                                                                                                     >  1:752315/1‑150 (MQ=255)                               cAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGc                                                                                                                  <  1:585059/151‑1 (MQ=255)                                aGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCg                                                                                                                 >  2:255470/1‑151 (MQ=255)                                aGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCg                                                                                                                 <  1:402207/151‑1 (MQ=255)                                  ccTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGt                                                                                                                <  2:176436/150‑1 (MQ=255)                                    ttACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGtga                                                                                                              >  1:369687/1‑150 (MQ=255)                                     tACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGtgatg                                                                                                            >  1:504603/1‑151 (MQ=255)                                       cccGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGtgatga                                                                                                           <  1:564200/150‑1 (MQ=255)                                       cccGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTgatgat                                                                                                          >  2:611835/1‑151 (MQ=255)                                         cGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTc                                                                                                        >  2:681706/1‑151 (MQ=255)                                          gCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCt                                                                                                       <  1:307782/151‑1 (MQ=255)                                          gCTGTCGCAGCCGCTTACCAGCCGCCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGCTAGCCCGCAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGCTGATTc                                                                                                        >  1:337749/1‑150 (MQ=255)                                             gTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTg                                                                                                      <  1:312348/149‑1 (MQ=255)                                             gTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTg                                                                                                      <  1:242088/149‑1 (MQ=255)                                              tCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTa                                                                                                    >  1:614862/1‑150 (MQ=255)                                               cGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTaa                                                                                                   <  2:732842/150‑1 (MQ=255)                                               cGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCAATACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAACCCACAAGCGTGATGATTCTGTAAg                                                                                                  <  1:656081/151‑1 (MQ=255)                                                     cGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTggg                                                                                            <  2:235660/151‑1 (MQ=255)                                                     cGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTggg                                                                                            >  2:183255/1‑151 (MQ=255)                                                          aCCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCcgtc                                                                                       <  2:362867/151‑1 (MQ=255)                                                           ccAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCcgtcg                                                                                      <  2:48870/151‑1 (MQ=255)                                                            cAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCcgtcgt                                                                                     <  2:435823/151‑1 (MQ=255)                                                            cAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCcgtcgt                                                                                     <  2:223886/151‑1 (MQ=255)                                                              gCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCt                                                                                   <  2:594038/151‑1 (MQ=255)                                                                    aaaCCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCa                                                                              >  2:268960/1‑150 (MQ=255)                                                                     aaCCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAAc                                                                            <  1:251168/151‑1 (MQ=255)                                                                             aaaTCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGAGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCttt                                                                    <  2:127985/151‑1 (MQ=255)                                                                               aTCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTgg                                                                  <  2:802673/151‑1 (MQ=255)                                                                                   ttACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGggtgg                                                              >  1:243846/1‑151 (MQ=255)                                                                                      ccGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAg                                                           <  1:816583/151‑1 (MQ=255)                                                                                       cGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGc                                                          <  1:32081/151‑1 (MQ=255)                                                                                          tCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGt                                                       >  1:182344/1‑151 (MQ=255)                                                                                                 gcTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCtga                                                <  2:863549/151‑1 (MQ=255)                                                                                                    cGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCtgaa                                               <  2:953967/149‑1 (MQ=255)                                                                                                       ccGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCtgaatgaa                                           >  2:275919/1‑150 (MQ=255)                                                                                                       ccGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCtgaat                                              <  2:382982/147‑1 (MQ=255)                                                                                                       ccGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCtgaat                                              >  1:382960/1‑147 (MQ=255)                                                                                                         gAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATc                                        >  1:530689/1‑151 (MQ=255)                                                                                                           aTGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATccc                                      >  1:440476/1‑151 (MQ=255)                                                                                                              ccGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGc                                    >  1:224480/1‑150 (MQ=255)                                                                                                               cGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAg                                  <  2:75356/151‑1 (MQ=255)                                                                                                                gCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAgg                                 >  1:107221/1‑151 (MQ=255)                                                                                                                     gCTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAat                            >  1:728172/1‑151 (MQ=255)                                                                                                                      cTGAACTTTGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAat                            >  2:225567/1‑150 (MQ=255)                                                                                                                         aaCTTTATCGTTAGGTCGAAAGCGATCGCCAAAATTCGTCAGTTGCTGAAAACCCTCAAGCGTGATGATTCTGTAAGCCTGGGGCGTCGTCTGCGCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATAtt                         <  1:532073/150‑1 (MQ=255)                                                                                                                             ttGTCGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAgc                     <  2:729167/150‑1 (MQ=255)                                                                                                                                 cGTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAg                <  1:685635/151‑1 (MQ=255)                                                                                                                                  gTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGc               >  2:255925/1‑151 (MQ=255)                                                                                                                                  gTTAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGc               <  1:543462/151‑1 (MQ=255)                                                                                                                                    tAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCt              <  2:261755/150‑1 (MQ=255)                                                                                                                                    tAGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTg             >  2:785876/1‑151 (MQ=255)                                                                                                                                     aGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTgg            <  1:163766/151‑1 (MQ=255)                                                                                                                                     aGCTCGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTg             <  2:680880/150‑1 (MQ=255)                                                                                                                                      gcccGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCAAGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGa           <  1:541157/148‑1 (MQ=255)                                                                                                                                       atCGCAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAGTGAAATCCCGCAGGAACATATTCAGCGCGAGCTGGAt          >  1:92162/2‑151 (MQ=255)                                                                                                                                         cGAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATc         <  2:337519/150‑1 (MQ=255)                                                                                                                                         cGAAAGCGCTCGCCAAAATTCGTCAGTTGCTAAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATc         <  1:166857/150‑1 (MQ=255)                                                                                                                                          gAAAGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGc       >  2:117908/1‑151 (MQ=255)                                                                                                                                            aaGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCAt     >  2:90286/1‑151 (MQ=255)                                                                                                                                             aGCGCTCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATg    <  2:664818/151‑1 (MQ=255)                                                                                                                                              gcgctcgcCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGa   <  2:846863/151‑1 (MQ=255)                                                                                                                                              gcgctcgcCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGa   <  2:209070/151‑1 (MQ=255)                                                                                                                                               cgctcgcCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGaa  <  2:64461/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                    CATGCCTGCGTGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCGAAAGCGCGCGCCAAAATTCGTCAGTTGCTGAAAAACCTCAAGCGTGATGATTCTGTAAGCCTGGGCCGTCGTCTGCTCAACCATGCTTTGGGTGGTAGCCGTAAGCTGAATGAAATCCCGCAGGAAAATATTCAGCGCGAGCTGGATCGCATGAA  >  NC_000913/3823654‑3823946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑