Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,522,146	T→A	D650V (GAT→GTT)	yjhG ←	putative dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,522,146	0	T	A	100.0%	228.5 / NA	59	D650V (GAT→GTT)	yjhG	putative dehydratase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (33/26);  total (33/26)

CGATAATTAGTGGCATAACGTCCTCTCATAACAGGGTAGCGACCCATCATTATCCGACTGAGCAAATAACGAATGATGCAGAGTCGGATAATTCAGGTGTCTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAATCGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGCCTGAAGCATTGCCCATAGCCGGGTATCATCTGGCAATTCAGGATCGGGAAGTAAATCCTGATGGCTGGGTCTGGCATT  >  NC_000913/4522005‑4522291                                                                                                                                              |                                                                                                                                                  ctaTAATTAGTGGCATAACGTCCTCTCATAACAGGGTAGCGACCCATCATTATCCGACTGAGCAAATAACGAATGATGCAGAGTCGGATAATTCAGGTGTCTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAAACGCGCaa                                                                                                                                            >  2:519105/3‑149 (MQ=255) cGATAATTAGTGGCATAACGTCCTCTCATAACAGGGTAGCGACCCATCATTATCCGACTGAGCAAATAACGAATGATGCAGAGTCGGATAATTCAGGTGTCTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAAACGCGCaa                                                                                                                                            >  1:902985/1‑149 (MQ=255)   aTAATTAGTGGCATAACGTCCTCTCATAACAGGGTAGCGACCCATCATTATCCGACTGAGCAAATAACGAATGATGCAGAGTCGGATAATTCAGGTGTCTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGcc                                                                                                                                        <  1:631316/151‑1 (MQ=255)      aTTAGTGGCATAACGTCCTCTCATAACAGGGTAGCGACCCATCATTATCCGACTGAGCAAATAACGAATGATGCAGAGTCGGATAATTCAGGTGTCTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCcgcg                                                                                                                                     >  2:660206/1‑151 (MQ=255)       ttAGTGGCATAACGTCCTCTCATAACAGGGTAGCGACCCATCATTATCCGACTGAGCAAATAACGAATGATGCAGAGTCGGATAATTCAGGTGTCTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCCgcgc                                                                                                                                    >  2:51986/1‑151 (MQ=255)           tGGCATAACGTCCTCTCATAACAGGGTAGCGACCCATCATTATCCGACTGAGCAAATAACGAATGATGCAGAGTCGGATAATTCAGGTGTCTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAAt                                                                                                                                >  1:854013/1‑151 (MQ=255)               aTAACGTCCTCTCATAACAGGGTAGCGACCCATCATTATCCGACTGAGCAAATAACGAATGATGCAGAGTCGGATAATTCAGGTGTCTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAAtttt                                                                                                                             >  1:536210/1‑150 (MQ=255)                      ccTCTCATAACAGGGTAGCGACCCATCATTATCCGACTGAGCAAATAACGAATGATGCAGAGTCGGATAATTCAGGTGTCTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCTGCGCCAATTTTGTTTAcat                                                                                                                     >  2:260994/1‑151 (MQ=255)                      ccTCTCATAACAGGGTAGCGACCCATCATTATCCGACTGAGCAAATAACGAATGATGCAGAGTCGGATAATTCAGGTGTCTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTAca                                                                                                                      <  1:742188/150‑1 (MQ=255)                        tctcATAACAGGGTAGCGACCCATCATTATCCGACTGAGCAAATAACGAATGATGCAGAGTCGGATAATTCAGGTGTCTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATGAAAACGCGCAAAGCCGCGCCAATTTTGTTTAcatc                                                                                                                    >  1:50483/1‑150 (MQ=255)                          tcATAACAGGGTAGCGACCCATCATTATCCGACTGAGCAAATAACGAATGATGCAGAGTCGGATAATTCAGGTGTCTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTAcatcat                                                                                                                  >  2:707932/1‑150 (MQ=255)                           cATAACAGGGTAGCGACCCATCATTATCCGACTGAGCAAATAACGAATGATGCAGAGTCGGATAATTCAGGTGTCTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTAcatca                                                                                                                   <  2:954116/148‑1 (MQ=255)                              aaCAGGGTAGCGACCCATCATTATCCGACTGAGCAAATAACGAATGATGCAGAGTCGGATAATTCAGGTGTCTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAAt                                                                                                              <  1:946160/150‑1 (MQ=255)                              aaCAGGGTAGCGACCCATCATTATCCGACTGAGCAAATAACGAATGATGCAGAGTCGGATAATTCAGGTGTCTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATa                                                                                                             <  1:248805/151‑1 (MQ=255)                                      aGCGACCCATCATTATCCGACTGAGCAAATAACGAATGATGCAGAGTCGGATAATTCAGGTGTCTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGt                                                                                                     >  2:376863/1‑151 (MQ=255)                                        cGACCCATCATTATCCGACTGAGCAAATAACGAATGATGCAGAGTCGGATAATTCAGGTGTCTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTcc                                                                                                   >  1:559392/1‑151 (MQ=255)                                         gACCCATCATTATCCGACTGAGCAAATAACGAATGATGCAGAGTCGGATAATTCAGGTGTCTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCa                                                                                                  <  2:546969/151‑1 (MQ=255)                                          aCCCATCATTATCCGACTGAGCAAATAACGAATGATGCAGAGTCGGATAATTCAGGTGTCTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCAt                                                                                                 >  2:363309/1‑151 (MQ=255)                                           cccATCATTATCCGACTGAGCAAATAACGAATGATGCAGAGTCGGATAATTCAGGTGTCTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATg                                                                                                <  2:503648/151‑1 (MQ=255)                                             catcatTATCCGACTGAGCAAATAACGAATGATGCAGAGTCGGATAATTCAGGTGTCTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTc                                                                                              >  2:278514/1‑151 (MQ=255)                                                 atTATCCGACTGAGCAAATAACGAATGATGCAGAGTCGGATAATTCAGGTGTCTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCccac                                                                                          >  1:66452/1‑151 (MQ=255)                                                           tGAGCAAATAACGAATGATGCAGAGTCGGATAATTCAGGTGTCTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGc                                                                                >  2:210461/1‑151 (MQ=255)                                                             aGCAAATAACGAATGATGCAGAGTCGGATAATTCAGGTGTCTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGCCt                                                                              <  2:451661/151‑1 (MQ=255)                                                              gCAAATAACGAATGATGCAGAGTCGGATAATTCAGGTGTCTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGCCTg                                                                             <  1:753963/151‑1 (MQ=255)                                                                        aaTGATGCAGAGTCGGATAATTCAGGTGTCTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGCCTGAAGCATTGcc                                                                   <  2:359715/151‑1 (MQ=255)                                                                            atgCAGAGTCGGATAATTCAGGTGTCTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGCCTGAAGCATTGCCCAt                                                                >  2:782569/1‑150 (MQ=255)                                                                            atgCAGAGTCGGATAATTCAGGTGTCTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGCCTGAAGCATTGCCCATa                                                               <  2:258604/151‑1 (MQ=255)                                                                              gCAGAGTCGGATAATTCAGGTGTCTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGCCTGAAGCATTGCCCATAg                                                              <  1:812980/150‑1 (MQ=255)                                                                              gCAGAGTCGGATAATTCAGGTGTCTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGCCTGAAGCATTGCCCATAg                                                              <  1:97164/150‑1 (MQ=255)                                                                                agagTCGGATAATTCAGGTGTCTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGCCTGAAGCATTGCCCATAGCCg                                                           >  2:310661/1‑151 (MQ=255)                                                                                  agTCGGATAATTCAGGTGTCTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGCCTGAAGCATTGCCCATAGCCggg                                                         >  2:196451/1‑151 (MQ=255)                                                                                     cGGATAATTCAGGTGTCTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGCCTGAAGCATTGCCCATAGCCGGGTat                                                      <  2:74643/151‑1 (MQ=255)                                                                                          aaTTCAGGTGTCTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGCCTGAAGCATTGCCCATAGCCGGGTATCATCt                                                 <  2:450980/151‑1 (MQ=255)                                                                                           aTTCAGGTGTCTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGCCTGAAGCATTGCCCATAGCCGGGTATCATCTg                                                <  1:850979/151‑1 (MQ=255)                                                                                               aGGTGTCTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGCCTGAAGCATTGCCCATAGCCGGGTATCATCTGGCaa                                            <  2:891749/151‑1 (MQ=255)                                                                                                     cTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGCCTGAAGCATTGCCCATAGCCGGGTATCATCTGGCAATTCAgg                                      >  1:17336/1‑151 (MQ=255)                                                                                                          tGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGCCTGAAGCATTGCCCATAGCCGGGAATCATCTGGCAATTCAGGATCg                                  >  1:162322/1‑150 (MQ=255)                                                                                                             ttttATTTATGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGCCTGAAGCATTGCCCATAGCCGGGTATCATCTGGCAATTCAGGATCGGGa                               >  2:569101/1‑150 (MQ=255)                                                                                                              tttatttaTGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGCCTGAAGCATTGCCCATAGCCGGGTATCATCTGGCAATTCAGGATCGGGa                               >  1:826861/1‑149 (MQ=255)                                                                                                                tatttatGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGCCTGAAGCATTGCCCATAGCCGGGTATCATCTGGCAATTCAGGATCGGGAAGt                            >  2:317276/1‑150 (MQ=255)                                                                                                                     atGCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGCCTGAAGCATTGCCCATAGCCGGGTATCATCTGGCAATTCAGGATCGGGAAGTAAATcc                      <  2:668348/151‑1 (MQ=255)                                                                                                                       gCTTTCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGCCTGAAGCATTGCCCATAGCCGGGTATCATCTGGCAATTCAGGATCGGGAAGTAAATCCTg                    <  1:660168/151‑1 (MQ=255)                                                                                                                        cTTTCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCACACCACTCACGGCCTGAAGCATTGCCCATAGCCGGGTATCATCTGGCAATTCAGGTTCGGGAAGTAAATCATg                    >  1:314136/1‑150 (MQ=255)                                                                                                                          ttCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGCCTGAAGCATTGCCCATAGCCGGGTATCATCTGGCAATTCAGGATCGGGAAGTAAATCCTGAt                  <  2:311000/150‑1 (MQ=255)                                                                                                                          ttCAGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGCCTGAAGCATTGCCCATAGCCGGGTATCATCTGGCAATTCAGGATCGGGAAGTAAATCCTGATg                 >  2:896808/1‑151 (MQ=255)                                                                                                                             aGTTTTTATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGCCTGAAGCATTGCCCATAGCCGGGTATCATCTGGCAATTCAGGATCGGGAAGTAAATCCTGATGGCt              <  2:386958/151‑1 (MQ=255)                                                                                                                                  ttATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGCCTGAAGCATTGCCCATAGCCGGGTATCATCTGGCAATTCAGGATCGGGAAGTAAATCCTGATGGCTGGGt          <  1:740285/150‑1 (MQ=255)                                                                                                                                  ttATTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGCCTGAAGCATTGCCCATAGCCGGGTATCATCTGGCAATTCAGGATCGGGAAGTAAATCCTGATGGCTGGGTc         >  2:525204/1‑151 (MQ=255)                                                                                                                                    aTTCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGCCTGAAGCATTGCCCATAGCCGGGTATCATCTGGCAATTCAGGATCGGGAAGTAAATCCTGATGGCTGGGTCTg       >  2:510793/1‑151 (MQ=255)                                                                                                                                     ttCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATAAAACCGGTCCATGTCCCACCACTCACGGCCTGAAGCATTGCCCATAGCCGGGTATCCTCTGGCAATTCAGGATTGGGCAGCAAATCCTGATGGCTGGATCTgg      >  1:174718/1‑151 (MQ=255)                                                                                                                                      tCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGCCTGAAGCATTGCCCATAGCCGGGTATCATCTGGCAATTCAGGATCGGGAAGTAAATCCTGATGGCTGGGTCTGGc     >  2:814165/1‑151 (MQ=255)                                                                                                                                      tCATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGCCTGAAGCATTGCCCATAGCCGGGTATCATCTGGCAATTCAGGATCGGGAAGTAAATCCTGATGGCTGGGTCTGGc     >  2:895921/1‑151 (MQ=255)                                                                                                                                       cATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGCCTGAAGCATTGCCCATAGCCGGGTATCATCTGGCAATTCAGGATCGGGAAGTAAATCCTGATGGCTGGGTCTGGc     <  2:738845/150‑1 (MQ=255)                                                                                                                                       cATAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGCCTGAAGCATTGCCCATAGCCGGGTATCATCTGGCAATTCAGGATCGGGAAGTAAATCCTGATGGCTGGGTCTGGCa    <  1:196440/151‑1 (MQ=255)                                                                                                                                        aTAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGCCTGAAGCATTGCCCATAGCCGGGTATCATCTGGCAATTCAGGATCGGGAAGTAAATCCTGATGGCTGGGTCTGGCAt   <  1:544413/151‑1 (MQ=255)                                                                                                                                        aTAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGCCTGAAGCATTGCCCATAGCCGGGTATCATCTGGCAATTCAGGATCGGGAAGTAAATCCTGATGGCTGGGTCTGGCAt   >  2:884471/1‑151 (MQ=255)                                                                                                                                         tAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGCCTGAAGCATTGCCCATAGCCGGGTATCATCTGGCAATTCAGGATCGGGAAGTAAATCCTGATGGCTGGGTCTGGCAtt  >  2:209582/1‑151 (MQ=255)                                                                                                                                         tAAAAACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGCCTGAAGCATTGCCCATAGCCGGGTATCATCTGGCAATTCAGGATCGGGAAGTAAATCCTGATGGCTGGGTCTGGCAtt  >  1:730994/1‑151 (MQ=255)                                                                                                                                          aaaaACGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGCCTGAAGCATTGCCCATAGCCGGGTATCATCTGGCAATTCAGGATCGGGAAGTAAATCCTGATGGCTGGGTCTGGCAtt  <  2:880819/150‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                  CGATAATTAGTGGCATAACGTCCTCTCATAACAGGGTAGCGACCCATCATTATCCGACTGAGCAAATAACGAATGATGCAGAGTCGGATAATTCAGGTGTCTGGATGTTTTTATTTATGCTTTCAGTTTTTATTCATAAAATCGCGCAAAGCCGCGCCAATTTTGTTTACATCATAAATACAACCGGTCCATGTCCCACCACTCACGGCCTGAAGCATTGCCCATAGCCGGGTATCATCTGGCAATTCAGGATCGGGAAGTAAATCCTGATGGCTGGGTCTGGCATT  >  NC_000913/4522005‑4522291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑