Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,401,016	A→C	I24M (ATT→ATG)	mreB ←	cell wall structural complex MreBCD, actin‑like component MreB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,401,016	0	A	C	100.0%	195.5 / NA	48	I24M (ATT→ATG)	mreB	cell wall structural complex MreBCD, actin‑like component MreB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (29/19);  total (29/19)

ATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGGAAAAGAAAATCCGCTTACTTTACCAACCACACGCAGCAGCGACA  >  NC_000913/3400870‑3401161                                                                                                                                                   |                                                                                                                                                  aTGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATACATg                                                                                                                                                >  1:673374/1‑150 (MQ=255) aTGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATACATg                                                                                                                                                >  1:395213/1‑150 (MQ=255)        caATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATACATGAGGGTAtt                                                                                                                                        >  1:402264/1‑151 (MQ=255)            atTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATACATGAGGGTATTCGc                                                                                                                                     >  2:735622/1‑150 (MQ=255)               gCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATACATGAGGGTATTCGCAGTa                                                                                                                                 <  1:549372/151‑1 (MQ=255)                cccGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATACATGAGGGTATTCGCAGTAc                                                                                                                                >  1:417450/1‑151 (MQ=255)                       tACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATACATGAGGGTATTCGCAGTACCCAGGTc                                                                                                                         >  2:333690/1‑151 (MQ=255)                       tACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATACATGAGGGTATTCGCAGTACCCAGGTc                                                                                                                         >  1:540519/1‑151 (MQ=255)                        aCGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATACATGAGGGTATTCGCAGTACCCAGGTc                                                                                                                         <  2:82742/150‑1 (MQ=255)                          ggCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATACATGAGGGTATTCGCAGTACCCAGGTCAAt                                                                                                                      <  2:192874/151‑1 (MQ=255)                                gCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATACATGAGGGTATTCGCAGTACCCAGGTCAATGGACaa                                                                                                                <  2:586559/151‑1 (MQ=255)                                 cATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATACATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAg                                                                                                               <  1:595953/151‑1 (MQ=255)                                  aTCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATACATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGt                                                                                                              >  1:181664/1‑151 (MQ=255)                                    cTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATACATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTc                                                                                                             >  2:73098/1‑150 (MQ=255)                                      gCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATACATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCa                                                                                                            <  1:738575/149‑1 (MQ=255)                                      gCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATACATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCAtt                                                                                                          >  2:602837/1‑151 (MQ=255)                                                   aCCTTCTTCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTATTACATACATGAGGGTATTCGGTGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGca                                                                                              <  1:472635/150‑2 (MQ=255)                                                   aCCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATACATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCa                                                                                             <  2:160311/151‑1 (MQ=255)                                                       aCTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATACATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGaa                                                                                         <  2:834520/151‑1 (MQ=255)                                                       aCTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATACATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGa                                                                                          >  2:700546/1‑150 (MQ=255)                                                       aCTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATACATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGa                                                                                          >  1:643935/1‑150 (MQ=255)                                                         tGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATACATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAAt                                                                                       >  1:907189/1‑151 (MQ=255)                                                             gCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCAAGGAAGGCTCATTCAATACGATGCCTTGTCATTTTACATACATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAAttttt                                                                                   >  2:666238/1‑151 (MQ=255)                                                                        cGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATACATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACt                                                                         <  1:110923/150‑1 (MQ=255)                                                                            gAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATACATGAGGGTATTCGCAGTACCCAGGTCAATGGACGAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAgg                                                                     <  2:47600/150‑1 (MQ=255)                                                                                   cACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATACATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATcc                                                             >  2:90450/1‑151 (MQ=255)                                                                                    aCGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTTCATACATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCt                                                            >  2:743329/1‑151 (MQ=255)                                                                                     cGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATACATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTg                                                           >  2:761550/1‑151 (MQ=255)                                                                                       aTCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATACATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGaa                                                         <  2:263207/151‑1 (MQ=255)                                                                                           tGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATACATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCt                                                     >  2:46156/1‑151 (MQ=255)                                                                                                   ggCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATACATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGga                                             <  2:530835/151‑1 (MQ=255)                                                                                                          acGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATACATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGgaaaagaa                                       >  2:174969/1‑150 (MQ=255)                                                                                                           cGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATACATGAGGGTATTCGCAGTACCTAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGgaaaagaaa                                      >  2:379715/1‑150 (MQ=255)                                                                                                            ggAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATACATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGGAAAAGAAAAt                                    >  2:749744/1‑151 (MQ=255)                                                                                                            ggAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATACATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGGAAAAGAAAAt                                    <  1:869472/151‑1 (MQ=255)                                                                                                              aaGGCTCATTCAATACGATGCCTTGTCCTTTTACATACATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGGAAAAGAAAATcc                                  <  1:494994/151‑1 (MQ=255)                                                                                                              aaGGCTCATTCAATACGATGCCTTGTCCTTTTACATACATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGGAAAAGAAAATcc                                  >  1:108725/1‑151 (MQ=255)                                                                                                                      ttCAATACGAAGCCTTGTCCTTTTACATACATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGTGCGGAAAAGAAAATCCGCTTACtt                          >  1:622883/1‑151 (MQ=255)                                                                                                                               aTGCCTTGTCCTTTTACATACATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGGAAAAGAAAATCCGCTTACTTTaccaacc                  >  2:110668/1‑150 (MQ=255)                                                                                                                               aTGCCTTGTCCTTTTACATACATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGGAAAAGAAAATCCGCTTACTTTaccaacc                  >  1:351239/1‑150 (MQ=255)                                                                                                                               aTGCCTTGTCCTTTTACATACATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAATCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGGAAAAGAAAATCCGCTTACTTTaccaacc                  >  1:486817/1‑150 (MQ=255)                                                                                                                                 gCCTTGTCCTTTTACATACATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGGAAAAGAAAATCCGCTTACTTTACCAACcaca               <  1:333670/151‑1 (MQ=255)                                                                                                                                    ttGTCCTTTTACATACATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGGAAAAGAAAATCCGCTTACTTTACCAACCACACgc            >  2:701127/1‑151 (MQ=255)                                                                                                                                      gTCCTTTTACATACATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGGAAAAGAAAATCCGCTTACTTTACCAACCACACgcag          <  2:159356/151‑1 (MQ=255)                                                                                                                                      gTCCTTTTACATACATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGGAAAAGAAAATCCGCTTACTTTACCAACCACACgca           <  2:613619/150‑1 (MQ=255)                                                                                                                                      gTCCTTTTACATACATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGGAAAAGAAAATCCGCTTACTTTACCAACCACACgc            >  2:307134/1‑149 (MQ=255)                                                                                                                                           tttACATACATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGGAAAAGAAAATCCGCTTACTTTACCAACCACACGCagcagc      <  2:803533/150‑1 (MQ=255)                                                                                                                                               cATACATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGGAAAAGAAAATCCGCTTACTTTACCAACCACACGCAGCAGCGACa  >  1:510477/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                  ATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGGAAAAGAAAATCCGCTTACTTTACCAACCACACGCAGCAGCGACA  >  NC_000913/3400870‑3401161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑