Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,153,573 | 0 | T | G | 46.4% | 78.1 / 27.1 | 56 | Y92D (TAC→GAC) | pabC | 4‑amino‑4‑deoxychorismate lyase component of para‑aminobenzoate synthase multienzyme complex |
Reads supporting (aligned to +/- strand): ref base T (0/30); new base G (26/0); total (26/30) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.50e-16 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGACTACAGGATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCA‑CATTGAACAGC‑GGACCGGCAACGC‑GGATTCTC‑TCCG‑TTACGGCTTAT‑CCTGCACATTACG‑ACCGTTTGCG‑TAACGAGGGGATTACGTTGGCGCT‑AAGCCCGGTGCGGCTGGGGCGCAATCCTCATCTTGCAGGTATTAAAC > NC_000913/1153432‑1153720 | cGACTACAGGATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGGGGGGGGAGAGcaaa > 1:381540/1‑148 (MQ=255) gACTACAGGATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCA‑CAtt < 2:202796/151‑1 (MQ=255) aCTACAGGATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCA‑CATTg < 1:294933/151‑1 (MQ=255) aCAGGATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGGGGGGGGAAAGca‑caat > 2:388867/1‑145 (MQ=255) aCAGGATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCA‑CATTGaa < 1:128707/150‑1 (MQ=255) aCAGGATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCA‑CATTGAAc < 2:235733/151‑1 (MQ=255) aCAGGATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCA‑CATTGAAc < 1:230480/151‑1 (MQ=255) cAGGATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCA‑CATTGAACa < 2:1008906/151‑1 (MQ=255) gATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGGGGGGGAAGca‑caattaa > 2:289864/1‑141 (MQ=255) aTGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGGGGGGGGGCAACA‑CAATGAACa > 1:856080/1‑147 (MQ=255) gCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCA‑CATTGAACAGC‑GGa < 1:983341/150‑1 (MQ=255) gCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCA‑CATTGAACAGC‑GGAc < 2:172237/151‑1 (MQ=255) ttGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGGGGGGGCAGCA‑CATAGaaa > 1:595005/1‑141 (MQ=255) tGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCA‑CATTGAACAGC‑GGACCg < 2:188594/150‑1 (MQ=255) gTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCA‑CATTGAACAGC‑GGACCgg < 1:918558/150‑1 (MQ=255) cAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCA‑CATTGAACAGC‑GGACCGGCaa < 2:300414/151‑1 (MQ=255) aGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAGGTCGTGATCAGTCGCGGTAGTGGCGGGCGGGGGGAGAGCA‑CAATGAACAGG‑GGAGCgg > 2:433741/1‑147 (MQ=255) ggTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGGGGGGGGAGAGGA‑CACTGAACAGC‑GGAcccg > 2:930345/1‑142 (MQ=255) ttGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGGAAAACA‑CATTGAACAAC‑GGACCGGGAAcgccg > 2:152605/1‑148 (MQ=255) atgatTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCA‑CATTGAACAGC‑GGACCGGCAACGC‑GGATtc < 1:186462/151‑1 (MQ=255) tcatgtgACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGACAGACGTGATCAGACGCGGCAGTGGCGGCGGCGGGGACAGGG‑CAATAAACAGCGGGTCCGGCaaaccga > 1:1047937/4‑137 (MQ=255) tCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCA‑CATTGAACAGC‑GGACCGGCAACGC‑GGATTCTC‑Tcc < 1:574057/150‑1 (MQ=255) tCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCA‑CATTGAACAGC‑GGACCGGCAACGC‑GGATTCTC‑Tcc < 1:417035/150‑1 (MQ=255) aCTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCA‑CATTGAACAGC‑GGACCGGCAACGC‑GGATTCTC‑TCCG‑TTACGGc < 1:577136/151‑1 (MQ=255) cAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCA‑CATTGAACAGC‑GGACCGGCAACGC‑GGATTCTC‑TCCG‑TTACGGCTTAT‑CCTGca < 2:387344/150‑1 (MQ=255) cAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCA‑CATTGAACAGC‑GGACCGGCAACGC‑GGATTCTC‑TCCG‑TTACGGCTTAT‑CCTGca < 1:208793/150‑1 (MQ=255) cAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCA‑CATTGAACAGC‑GGACCGGCAACGC‑GGATTCTC‑TCCG‑TTACGGCTTAT‑CCTGc < 2:147641/149‑1 (MQ=255) aGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCA‑CATTGAACAGC‑GGACCGGCAACGC‑GGATTCTC‑TCCG‑TTACGGCTTAT‑CCTGcaca < 2:507674/151‑1 (MQ=255) ttGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGGGGGGGGGCAGCA‑CAATTAACAAC‑GGGCCGGCAACGC‑GGGTTTTC‑TCCC‑TTACGGCCTAT‑TCTTcac > 2:994891/1‑147 (MQ=255) aaCAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGGGAGGGGGAAGCA‑CATTGAAAAGA‑GGGGCGGGAACGC‑GGGTTCTC‑TCCG‑TTTCGGGGTAT‑TCTGcac > 2:245545/1‑144 (MQ=255) aaCAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGGGGGGACAGCA‑CATTGAAAAAC‑GGACCGGCCACGC‑GGGGTCTC‑TCCC‑TTTCGGCTTTT‑CCTGcc > 1:614817/1‑142 (MQ=255) agagATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCA‑CATTGAACAGC‑GGACCGGCAACGC‑GGATTCTC‑TCCG‑TTACGGCTTAT‑CCTGCACATTACG‑ACCGt < 2:155311/151‑1 (MQ=255) aTGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCA‑CATTGAACAGC‑GGACCGGCAACGC‑GGATTCTC‑TCCG‑TTACGGCTTAT‑CCTGCACATTACG‑ACCGTTTGc < 1:501735/151‑1 (MQ=255) aaaaCGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCA‑CATTGAACAGC‑GGACCGGCAACGC‑GGATTCTC‑TCCG‑TTACGGCTTAT‑CCTGCACATTACG‑ACCGTTTGCG‑Ta < 1:116350/151‑1 (MQ=255) tGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGGGGGGGAAGCA‑CATTTAACAAC‑GGACCGGCGACGC‑CGGGTCTT‑TCCC‑CTACGGCTTTTCCCTGC‑CATTAAC‑ACCGCCTGCG‑TGACGAggg > 1:848931/1‑151 (MQ=255) agcagAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGGGGGGGGAGCA‑CAATGAAAAAC‑GGAGCGGCAACGC‑GGGGTTTC‑TCCG‑TTTCGGGCTTT‑TCTTCCCATTATC‑ACCCCTTGCG‑GAACCAgggg > 1:143401/1‑148 (MQ=255) agcagAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCA‑CATTGAACAGC‑GGACCGGCAACGC‑GGATTCTC‑TCCG‑TTACGGCTTAT‑CCTGCACATTACG‑ACCGTTTGCG‑TAACGAGGGGAtt < 1:889561/151‑1 (MQ=255) aaCAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCA‑CATTGAACAGC‑GGACCGGCAACGC‑GGATTCTC‑TCCG‑TTACGGCTTAT‑CCTGCACATTACG‑ACCGTTTGCG‑TAACGAGGGGATTACGtt < 2:120524/151‑1 (MQ=255) aaaaTGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGGGGGGGGAAGA‑CCATGAACAAC‑GGAGCGGGCACGCGGGGTTTTT‑CCCT‑TTACGCCTTTT‑CCCCCACATTCGA‑ACCCGTTG‑G‑TAAAGAGGGGGGTACGtttgggg > 1:787348/1‑145 (MQ=255) aaaaTGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCA‑CATTGAACAGC‑GGACCGGCAACGC‑GGATTCTC‑TCCG‑TTACGGCTTAT‑CCTGCACATTACG‑ACCGTTTGCG‑TAACGAGGGGATTACGTTGGCGCt < 1:937472/151‑1 (MQ=255) aaaTGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGAGGGGGGGGAGCA‑AATTCAACAGG‑GGAGCGGCCACGC‑GGGGTCTC‑CCCG‑TTTCGGCCTTT‑CCTGCCCATTACA‑ACCGGTTGAGTTAACGGGGGGGTTCCTTTgcg > 1:502402/1‑146 (MQ=255) tgtgCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGGGGGGGGACAACA‑CATTTAAAAGC‑GGACCGGGAACCC‑GGACTTTT‑TCCG‑CTATCGCCTAT‑TCTGCACAATTCT‑ACCCGTTGCG‑GTAAGAGGGGGGTATGTTGGCGAT‑CAGccc > 2:696323/1‑150 (MQ=255) gAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGGGGGGACAGCA‑CATTTAAAAGC‑GGGCCCGCCACGC‑GGGGTCTC‑TCCC‑CTTCGGCTTAT‑CCTGCACATTACG‑ACCGTTTTCG‑TGACGAGGGGGTTACGTTTGGGCT‑AAGCCCCGGGCgg > 2:658081/1‑151 (MQ=255) gAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGGACAGCA‑CCTTGAAAAGC‑GGGCCGGCCACGC‑GGGTTCTC‑TCCC‑TTTCGGCTTAT‑CCTCCACATTACG‑ACCCCTTGCG‑TAACGAGGGGGTTACGTTTGCGCT‑AAGCCCGGGGCgg > 2:614729/1‑151 (MQ=255) aaGTCGTGATCAGTCGCGGTAGTGGCGGGCGGGGGGGCAGCA‑CATTGAAAAAC‑GGAACGGGAAAGC‑GGGTTCTCTTCCG‑TTTGGGCTT‑T‑TCTGCCCACTACG‑ACCGCTTTGG‑TTACGAGGGGGTTACGTTTgcgcctagacccggggg > 2:485671/1‑135 (MQ=255) cGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCA‑CATTGAACAGC‑GGACCGGCAACGC‑GGATTCTC‑TCCG‑TTACGGCTTAT‑CCTGCACATTACG‑ACCGTTTGCG‑TAACGAGGGGATTACGTTGGCGCT‑AAGCCCGGTGCGGCTgggg < 2:442514/151‑1 (MQ=255) gTGATCAGTCGCGGTAGTGGCGGGGGGGGGGGCAACACCATTGAA‑AAA‑GGAGCGGCCACAC‑GGGGTCTC‑TCCG‑TTTCGGGCTTT‑CCTGCACAATTCG‑ACCGGTTGTG‑TAAACAGGGGGATGCGTTGGCGGT‑CAACCCCGGGCGGGCgggg > 1:944224/1‑150 (MQ=255) gTGATCAGTCGCGGTAGTGGCGGGCGGGGGGACAACA‑CATTGAACAGA‑GGGCCGGGAACGC‑GGGTTTTC‑TCCC‑TTTCGGGTTAT‑TCCTCACATTAAGAACCGTTT‑CG‑TAACGAGGGGGGTACGTTGGCGCTAAAGCCCggggggcgggggg > 2:332150/1‑140 (MQ=255) gTGATCAGTCGCGGTAGTGGCGGGCGAGGGGACAGCA‑CATTTAACAAC‑GGACCGGCAACGC‑GGGTTCTC‑TCCC‑TTACGGCTTTT‑CCTGCCCATTACG‑ACCGCTTTCG‑GAACGAGGGGGGTACGTTTGCGCT‑CAACCCCGGGGGGCGggggg > 2:84231/1‑150 (MQ=255) gTGATCAGTCGCGGTAGTGGCGGGCGAGGGGAAAGCA‑CCTTTAACAGC‑GGGCCGGCAACAC‑GGAGTCTC‑TCCC‑GTACGGCCTTT‑CCCGCACCTTAAG‑ACCGTTTTCG‑GAACGAGGGGGGTACGTTGGCGCT‑AAACCCGGCGCGGGTggggg > 2:955389/1‑150 (MQ=255) aGTCGCGGTAGTGGCGGGCGGGGGGAAAGAA‑CATTGAACCAA‑GGGGCGGCCAAGC‑GGGGTGTC‑TCCGCTTAC‑GCGTAT‑TCCGCACATTTTC‑ACCGTTTGTG‑GCACGAGGGGGGTACGTTTGGGCT‑TAACCCGGGGCGGGCggggggggatc > 1:764486/1‑144 (MQ=255) cgcgGTAGTGGCGGGCGGGGGGACAGCA‑CATTGAACAGC‑GGACCGGGAACGC‑GGGTTCTC‑TCCG‑TTTCGGCGTTT‑CCTTCACATTACC‑ACCGTTTTCG‑TAACGAGGGGGATAAGTTGGCGGT‑AAAGCCCGTGGGGGTGGGgggccaacc > 2:990391/1‑144 (MQ=255) cgcgGTAGTGGCGGGCGAGGGTACAGCA‑CTTTGAACAGC‑GGACCGGCAACGC‑GGATTCTC‑TCCG‑TTACGGCTTAT‑CCTGCACATTACG‑ACCGTTTGCG‑TAACGAGGGGATTACGTTGGCGCT‑AAGCCCGGTGCGGTTGGGGCGCAATCCt < 1:686161/150‑1 (MQ=255) cgcgGTAGTGGCGGGCGAGGGTACAGCA‑CATTGAACAGC‑GGACCGGCAACGC‑GGATTCTC‑TCCG‑TTACGGCTTAT‑CCTGCACATTACG‑ACCGTTTGCG‑TAACGAGGGGATTACGTTGGCGCT‑AAGCCCGGTGCGGCTGGGGCGCAATCCTc < 2:345395/151‑1 (MQ=255) cgcgGTAGTGGCGGGCGAGGGTACAGCA‑CATTGAACAGC‑GGACCGGCAACGC‑GGATTCTC‑TCCG‑TTACGGCTTAT‑CCTGCACATTACG‑ACCGTTTGCG‑TAACGAGGGGATTACGTTGGCGCT‑AAGCCCGGTGCGGCTGGGGCGCAATCCTc < 1:614819/151‑1 (MQ=255) gggTACAGCA‑CATTGAACAGC‑GGACCGGCAACGC‑GGATTCTC‑TCCG‑TTACGGCTTAT‑CCTGCACATTACG‑ACCGTTTGCG‑TAACGAGGGGATTACGTTGGCGCT‑AAGCCCGGTGCGGCTGGGGCGCAATCCTCATCTTGCAGGTATTAAAc < 1:388859/151‑1 (MQ=255) | CGACTACAGGATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCA‑CATTGAACAGC‑GGACCGGCAACGC‑GGATTCTC‑TCCG‑TTACGGCTTAT‑CCTGCACATTACG‑ACCGTTTGCG‑TAACGAGGGGATTACGTTGGCGCT‑AAGCCCGGTGCGGCTGGGGCGCAATCCTCATCTTGCAGGTATTAAAC > NC_000913/1153432‑1153720 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |