Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,903,692	0	C	A	42.9%	101.2 / 58.4	70	intergenic (+318/+1055)	ygcE/queE	putative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
Reads supporting (aligned to +/- strand):  ref base C (3/37);  new base A (30/0);  total (33/37)
Fisher's exact test for biased strand distribution p-value = 9.86e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATT‑AGCCT‑C‑AAAACAAAGACAAAC‑T‑TCC‑AGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACG  >  NC_000913/2903546‑2903841                                                                                                                                                   |                                                                                                                                                             ttACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCAc                                                                                                                                                           <  1:554479/150‑1 (MQ=255)    cAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCAt                                                                                                                                                       <  2:852156/151‑1 (MQ=255)     aaGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCAtt                                                                                                                                                      <  2:67530/151‑1 (MQ=255)      aGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATT‑a                                                                                                                                                    <  2:216258/151‑1 (MQ=255)       ggAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACACCCAtt                                                                                                                                                      >  2:282676/1‑149 (MQ=255)                 cTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAAAACTCATT‑ATACT‑C‑caaa                                                                                                                                         >  1:832810/1‑149 (MQ=255)                    cacTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTTAAAACAAACATT‑ATACC‑C‑aaaaaaaa                                                                                                                                     >  1:154167/1‑150 (MQ=255)                    cacTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATT‑AGCCT‑C‑AAAACAAAg                                                                                                                                    <  2:907649/151‑1 (MQ=255)                         gATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATT‑AGCCT‑C‑AAAACAAAGACaaa                                                                                                                               <  2:496904/151‑1 (MQ=255)                               tttAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTGTAAAAACACTCATT‑TTCCT‑C‑CAAAAAAAAAAAAAA‑t‑ttc                                                                                                                        >  2:214197/1‑148 (MQ=255)                                ttAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATT‑AGCCT‑C‑AAAACAAAGACAAAC‑T‑TCC‑a                                                                                                                      <  1:824671/150‑1 (MQ=255)                                 tAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATT‑AGCCT‑C‑AAAACAAAGACAAAC‑T‑TCC‑Ag                                                                                                                     <  2:827757/150‑1 (MQ=255)                                  aGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATT‑AGCCT‑C‑AAAACAAAGACAAAC‑T‑TCC‑AGA‑t                                                                                                                  <  2:1012914/151‑1 (MQ=255)                                    aaTAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATT‑AGCCT‑C‑AAAACAAAGACAAAC‑T‑TCC‑AGA‑tt                                                                                                                 <  1:659024/150‑1 (MQ=255)                                     aTAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTTAAAAAACCCATT‑AACCT‑C‑AAAAAAAAAACAAAA‑A‑TCC‑CGA‑ttt                                                                                                                >  1:873904/1‑150 (MQ=255)                                     aTAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAAAACTCATT‑ATACT‑C‑AAAAAAAAAAAAAAA‑C‑TCC‑AAA‑ttt                                                                                                                >  1:726756/1‑150 (MQ=255)                                          aGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAAAACCCATT‑ATCCT‑C‑CAAAAAAAAAAAAAC‑A‑TCC‑AGA‑tttttt                                                                                                             >  1:335119/1‑148 (MQ=255)                                             cAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATT‑AGCCT‑C‑AAAACAAAGACAAAC‑T‑TCC‑AGA‑TTTATTACTTaa                                                                                                       <  1:59974/151‑1 (MQ=255)                                              aaTACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGGTTTGTAAAAACAAACTTT‑ATCCA‑C‑CAAAAAAAAACAAACAT‑TCC‑AAA‑tttttttttaa                                                                                                        >  1:26214/1‑145 (MQ=255)                                                 aCGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCGACAGCGTATAGAGGCGGTTTTTTTAAAACACTCATTAACCCC‑C‑AAAAAAAAAAAAAAA‑T‑TCC‑CGA‑TTTTTTTTTTAAca                                                                                                     >  1:482225/1‑149 (MQ=255)                                                   gAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTATAAACACGACTT‑TGACG‑C‑AAAATAAAGAACAAC‑TATCA‑AGA‑Tttattgttaa                                                                                                        >  2:215815/1‑140 (MQ=255)                                                     aTCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAAAACTCAAT‑TTACT‑C‑AAAAAAAAAAAAAAC‑A‑TCC‑AAA‑tttttttatt                                                                                                         >  1:53820/1‑137 (MQ=255)                                                         cACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATT‑AGCCT‑C‑AAAACAAAGACAAAC‑T‑TCC‑AGA‑TTTATTACTTAACCAATGAATAAc                                                                                           <  1:919729/151‑1 (MQ=255)                                                          aCTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAAAACTCATT‑AACCT‑C‑CAAAAAAAAAAAAAA‑A‑TCC‑AAA‑TTTTTTATTTTAACAAAGAaaaaa                                                                                           >  2:545478/1‑149 (MQ=255)                                                          aCTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAAAAATCATT‑AGCCT‑C‑AAAAAAAAAAAAAAA‑A‑TCC‑AGA‑ATTTTTATTTTAACaaataaaaa                                                                                            >  1:11558/1‑142 (MQ=255)                                                          aCTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATT‑AGCCT‑C‑AAAACAAAGACAAAC‑T‑TCC‑AGA‑TTTATTACTTAACCAATGAATAACa                                                                                          <  1:288631/151‑1 (MQ=255)                                                            tttATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAAAACTCCTTAACCCC‑C‑AAAAAAAAAAAAAAA‑T‑TCC‑A‑A‑TTTTTTTCTTAAACaaaaaaaaaaaa                                                                                         >  2:100046/1‑140 (MQ=255)                                                             ttATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAAAAAAACATT‑AACCT‑C‑AAAAAAAAAAAAAAA‑A‑TCC‑AGA‑TTTATTTTTTAAACAATAAATAACaa                                                                                         >  2:418848/1‑149 (MQ=255)                                                                 cAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATT‑AGCCT‑C‑AAAACAAAGACAAAC‑T‑TCC‑AGA‑TTTATTACTTAACCAATGAATAACAAGCTTaa                                                                                   <  1:197398/151‑1 (MQ=255)                                                                 cAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATT‑AGCCT‑C‑AAAACAAAGACAAAC‑T‑TCC‑AGA‑TTTATTACTTAACCAATGAATAACAAGCTTa                                                                                    <  1:423388/150‑1 (MQ=255)                                                                  aaTTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATT‑AGCCT‑C‑AAAACAAAGACAAAC‑T‑TCC‑AGA‑TTTATTACTTAACCAATGAATAACAAGCTTaaa                                                                                  <  1:728185/151‑1 (MQ=255)                                                                     tttCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTTAAAACACCCATT‑AACCT‑C‑CAAAAAAAAACAAAA‑A‑TCC‑AGA‑TTTTTTTATTTACCAAAGAAAAAAAAACTTtaaaa                                                                                >  2:421770/1‑150 (MQ=255)                                                                        cACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATT‑AGCCT‑C‑AAAACAAAGACAAAC‑T‑TCC‑AGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAatat                                                                            <  1:707298/151‑1 (MQ=255)                                                                          cTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAACACCCCTT‑AACCT‑C‑CAAACAAAAAAAAAA‑T‑TCC‑AGA‑TTTATTTCTTTACCAATGAAAAAAAAACTTaaaaaaaa                                                                             >  2:239450/1‑146 (MQ=255)                                                                              aaGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAACAACCATT‑ATCCC‑C‑AAAAAAAAAACAAAA‑A‑TTC‑AGA‑TTTTTTTTTTAACCAAAAAATAAAAAAATTaaaaaaaaaaaagtt                                                                      >  1:977271/1‑142 (MQ=255)                                                                                      aCTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATT‑AGCCT‑C‑AAAACAAAGACAAAC‑T‑TCC‑AGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTaaa                                                              <  2:356386/151‑1 (MQ=255)                                                                                         ggATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATT‑AGCCT‑C‑AAAACAAAGACAAAC‑T‑TCC‑AGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAg                                                             <  1:401304/149‑1 (MQ=255)                                                                                          gATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATT‑AGCCT‑C‑AAAACAAAGACAAAC‑T‑TCC‑AGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGaaa                                                          <  1:244021/151‑1 (MQ=255)                                                                                          gATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATT‑AGCCT‑C‑AAAACAAAGACAAAC‑T‑TCC‑AGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGaaa                                                          <  2:476711/151‑1 (MQ=255)                                                                                            tatCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAAAACACATT‑ATCCT‑C‑AAAAAAAAAAAAAAA‑A‑TCC‑AGA‑TTTTTTATTTTAACAAAGAAAAAAAAAATTAAAAAAAATAATGTTATTTTTAaaaaaa                                                         >  1:181131/1‑150 (MQ=255)                                                                                            tatCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTGTAAAAAAACACATT‑ATCCT‑C‑CAAACAAAAAAAAAA‑A‑TTC‑CGA‑TTTTTTTCTTAAACAAATAAAAAAAAAAATAAAAAAAATAATTTTTTTTTATATaaaa                                                         >  1:511730/1‑150 (MQ=255)                                                                                             atCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAAAACACATT‑ATCCT‑C‑AAAAAAAAAAAAAAA‑A‑TCC‑CGA‑TTTTTTTCTTAAACAAAGAAAAAAAAAATTAAAAAAAAAAATTTTATTTTTaaaaaaaa                                                        >  1:422511/1‑150 (MQ=255)                                                                                              tCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAAAACACATT‑TTACC‑C‑AAAAAAAAAAAAAAA‑T‑TTC‑CGATTTTTTTATTTAACAAAT‑AAAAAAAAACTTAAAAAAAATAATTTTTTTTTaaaaaaaaaa                                                       >  1:806919/1‑150 (MQ=255)                                                                                               cTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTTAAAACACCCCTT‑ATCCC‑C‑CAAACAAAAAAAAAA‑A‑TCT‑CGA‑TTTTTTTTTTTAACAAATAATAAAAAAATTTAAAAAAAAACTTTTTTTTTTAaaaaaaaaa                                                      >  1:769492/1‑149 (MQ=255)                                                                                                 ttATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATT‑AGCCT‑C‑AAAACAAAGACAAAC‑T‑TCC‑AGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGaaaaaa                                                       <  2:331336/147‑1 (MQ=255)                                                                                                 ttATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATT‑AGCCT‑C‑AAAACAAAGACAAAC‑T‑TCC‑AGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAAtt                                                     <  1:285787/149‑1 (MQ=255)                                                                                                  tATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAAAAACACCTT‑ATCCT‑CAAAAAAAAAAAAAAAA‑T‑TCA‑CCA‑TTTTTTA‑TTAACCAAAAAAAAAAAAACTTAAAAAAAAAAATTTTTTTTTTAAAAAAAAAATaa                                                   >  2:923121/1‑150 (MQ=255)                                                                                                     tCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTTAAAACACCCCTT‑AACCT‑C‑AAAAAAAAAAAAAAA‑A‑TCCAAAA‑TTTTTTCTTTAACAAAAAAAAAAAAACTTTAAAAAAAAAATTTTTTTTTTAAAAAAAAAATaaaa                                                  >  2:53524/1‑148 (MQ=255)                                                                                                     tCATAAGAACTATTCATCAACAGCGTATAGAGGCGGCTTTGTAAAAACACTCAAT‑ATTCT‑C‑CAAAAAAAAAAAAAA‑C‑TCC‑AAA‑TTTTTTAATTTAACAAAAAATAAAAAAACTAAAAAAAAAAATTTTATTTTTAAGAAAAAAATAACa                                                 >  2:1019289/1‑149 (MQ=255)                                                                                                         aaGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATT‑AGCCT‑C‑AAAACAAAGACAAAC‑T‑TCC‑AGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCAtt                                           <  2:202517/151‑1 (MQ=255)                                                                                                            aaCTATTCATCAACAGCGTATAGAGGCGGTTCTGTTAAAACAACCCTT‑ATCCT‑C‑AAAAAAAAAAAAAAA‑A‑TTC‑AAA‑TTTTTTTATTTAACAAAAAAAAAAAAAAATAAAAAAAAAAATATTTTTTTTAAAAAAAAAATAAAAAGCATTaa                                         >  1:792004/1‑150 (MQ=255)                                                                                                             aCTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATT‑AGCCT‑C‑AAAACAAAGACAAAC‑T‑TCC‑AGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAAtt                                       <  1:320077/151‑1 (MQ=255)                                                                                                               tATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAACACCCATT‑AACCT‑C‑CAAACAAAAAAAAAA‑A‑TCC‑CAA‑TTTTTTATTTTAACAATAAAAAACAAACTTAAAAAAAATAATGTTTTTTTTAAAAAAAAAATAAAAAGCATTAAtt                                       >  2:631215/1‑149 (MQ=255)                                                                                                                  tcatcaACAGCGTATAGAGGCGGTTATGTAAAACCACTCATT‑AGCCT‑C‑AAAACAAAGACAAAC‑T‑TCC‑AGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAat                                   <  2:829070/150‑1 (MQ=255)                                                                                                                   catcaACAGCGTATAGAGGCGGTTTTTTAAAAAAACCCCTT‑ATCCT‑CAAAAAAAAAAAAAAAA‑T‑TCC‑A‑A‑ATTATTAATTTAACAAAAAAAAAAAAACTTAAAAAAAAAACTTTTTTTTTTAAAAAAAAAATAAAAAGCATTAATTTaa                                    >  1:513988/1‑148 (MQ=255)                                                                                                                   catcaACAGCGTATAGAGGCGGTTATGTAAAACCACTCATT‑AGCCT‑C‑AAAACAAAGACAAAC‑T‑TCC‑AGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAata                                  <  1:960443/150‑1 (MQ=255)                                                                                                                    atcaACAGCGTATAGAGGCGGTTTTTTAAAAACACCCCTT‑AACCTCC‑AAAAAAAAAAAAAAA‑T‑TCC‑AAT‑TTTTTTATTTAA‑CAAAAAAAAAAAAACTTAAAAAAAATAATTTTTTTTTTAAGAAAAAAATAAAAAGCATTAAATTAATAtt                                >  2:730084/1‑151 (MQ=255)                                                                                                                    atcaACAGCGTATAGAGGCGGTTATGTAAAACCACTCATT‑AGCCT‑C‑AAAACAAAGACAAAC‑T‑TCC‑AGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATAtt                                <  2:713634/151‑1 (MQ=255)                                                                                                                           gCGTATAGAGGCGGTTATGTAAAACCACTCATT‑AGCCT‑C‑AAAACAAAGACAAAC‑T‑TCC‑AGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTaaaaaaa                         <  1:282671/151‑1 (MQ=255)                                                                                                                            cGTATAGAGGCGGTTATGTAAAACCACTCATT‑AGCCT‑C‑AAAACAAAGACAAAC‑T‑TCC‑AGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTaaaaaaa                         <  1:704813/150‑1 (MQ=255)                                                                                                                               ataGAGGCGGTTATGTAAAACCACTCATT‑AGCCT‑C‑AAAACAAAGACAAAC‑T‑TCC‑AGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAaataa                      <  1:1033289/150‑1 (MQ=255)                                                                                                                                 agagGCGGTTATGTAAAACCACTCATT‑AGCCT‑C‑AAAACAAAGACAAAC‑T‑TCC‑AGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAataata                    <  2:464872/150‑1 (MQ=255)                                                                                                                                   agGCGGTTATGTAAAACCACTCATT‑AGCCT‑C‑AAAACAAAGACAAAC‑T‑TCC‑AGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGAt                 <  1:549253/151‑1 (MQ=255)                                                                                                                                    ggCGGTTATGTAAAACCACTCATT‑AGCCT‑C‑AAAACAAAGACAAAC‑T‑TCC‑AGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGAtt                <  2:335124/151‑1 (MQ=255)                                                                                                                                           aTGTAAAACCACTCATT‑AGCCT‑C‑AAAACAAAGACAAAC‑T‑TCC‑AGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAAttt         >  2:23804/1‑151 (MQ=255)                                                                                                                                            tGTAAAACCACTCATT‑AGCCT‑C‑AAAACAAAGACAAAC‑T‑TCC‑AGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTc        >  1:895149/1‑151 (MQ=255)                                                                                                                                             gTAAAACCACTCATT‑AGCCT‑C‑AAAACAAAGACAAAC‑T‑TCC‑AGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTc        <  2:714521/150‑1 (MQ=255)                                                                                                                                             gTAAAACCACTCATT‑AGCCT‑C‑AAAACAAAGACAAAC‑T‑TCC‑AGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTc        <  2:30710/150‑1 (MQ=255)                                                                                                                                                 aaCCACTCATT‑AGCCT‑C‑AAAACAAAGACAAAC‑T‑TCC‑AGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAAc   >  1:458308/1‑151 (MQ=255)                                                                                                                                                  aCCACTCATT‑AGCCT‑C‑AAAACAAAGACAAAC‑T‑TCC‑AGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACg  <  2:960625/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                             TTACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATT‑AGCCT‑C‑AAAACAAAGACAAAC‑T‑TCC‑AGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACG  >  NC_000913/2903546‑2903841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑