Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,508,657 | 0 | A | G | 45.6% | 76.2 / 15.8 | 59 | F386S (TTC→TCC) | yhfW | phosphopentomutase‑related metalloenzyme superfamily protein |
Reads supporting (aligned to +/- strand): ref base A (4/27); new base G (26/0); total (32/27) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.11e-12 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCC > NC_000913/3508519‑3508806 | gttTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGGGCGGGAAAAATcaca > 1:693383/1‑149 (MQ=255) ttttAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGGAACCCGTCGGAACAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGCGGCGCGAGGaaaaaaacaaaaacc > 2:732885/1‑141 (MQ=255) tttAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATc > 2:462851/1‑143 (MQ=255) ttAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAAATtaca > 2:342821/1‑145 (MQ=255) tAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGt < 1:563703/150‑1 (MQ=255) aGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAATTCACACACGGGAGcc > 2:643222/1‑151 (MQ=255) ggAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGGGCGGGAAAATTAacaaaccggcggcccc > 2:155314/1‑140 (MQ=255) gaTCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCa < 1:1026268/151‑1 (MQ=255) aTCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGCGGCGCGCGGGAAAAATCACACACGCGAGCCCCCacaaca > 2:759797/1‑150 (MQ=255) cAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAATTAACACACCGGAGCCCCCCCCTCAGa > 1:496142/1‑150 (MQ=255) cAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGaa < 2:632674/151‑1 (MQ=255) atatGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATTAAAAACCGTAACCCCcccaaccaaaa > 1:290813/1‑143 (MQ=255) atatGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATAAAACAACGGAGTCCCCACAACAGaaa > 1:447323/1‑150 (MQ=255) atatGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAATTTACACACGGTAACCCCCACAACAaaaa > 2:432502/1‑150 (MQ=255) atatGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATTACACACCGGAGCCCCCCCACCCGaaa > 1:725084/1‑150 (MQ=255) tGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAATACACACACGGTAGCCCCCCCATCCGCAAACgt > 1:562820/1‑151 (MQ=255) gCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATCACACACGGGAGCCCCCCCATCAGaaaaaggg > 2:59606/1‑146 (MQ=255) gCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCgtg < 2:758372/151‑1 (MQ=255) cAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCgtgg < 1:14932/151‑1 (MQ=255) aGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGCAAAAATCACCAACGGGAGCCCCCACACCAGAAAACggg > 2:786894/1‑147 (MQ=255) gATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCgtggtg < 2:699628/151‑1 (MQ=255) cATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGGAAAATTACAAAAAGGAACCCCCACCACAGAAAGCGGGGGGCGcac > 1:102197/1‑150 (MQ=255) gAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCt < 2:297770/151‑1 (MQ=255) gggTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAAAAACACACCGTAGACCCCCCCACACAAAAAGCGGTGCGCACACCCAGccg > 1:712034/1‑148 (MQ=255) aaCCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCg < 2:569439/151‑1 (MQ=255) ccGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAAGTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGatc < 1:417190/151‑1 (MQ=255) ccGTCGGATCAAAACTCATAGAGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATCACACACGGGAGCCCCCACATCAGAAAACGTGGGGGGCACACCCAGCGGCGGAGCGgtc > 2:254475/1‑151 (MQ=255) cGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGatca < 2:916572/151‑1 (MQ=255) gTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGatcat < 1:171177/151‑1 (MQ=255) aTCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAAACACACAAGGGAGCCCCCACAACCAAAAGCGGGGGGGGCCCACCCAGCTGCGTAGCGATCATccc > 1:740479/1‑149 (MQ=255) aTCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCtt < 1:455097/151‑1 (MQ=255) tCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCtt < 2:704871/150‑1 (MQ=255) cAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATCACACAAGGGAGCCCCCCCATCAGAAAGCGTGGGGGGCGCACCCAGCCGCGGAGCGATCATccc > 1:258088/1‑147 (MQ=255) aaaaCTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCt < 2:6474/151‑1 (MQ=255) aaaaCTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCt < 1:29440/151‑1 (MQ=255) aaaCTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTg < 1:868788/151‑1 (MQ=255) aaCTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATTACACACCGTAGCCCCCCCATCAAAAAACGGGGGGCGCCCACCCAGCTGCGTAGCGGTCATcccct > 1:356278/1‑144 (MQ=255) cATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATTACACACGGGAGCCCCCCCCACAGAAAGCGGGGGGCGCCCACCCAGCTGCGTAGCGATCATCCCTTTCTGATaa > 2:954472/1‑150 (MQ=255) cATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATCAAACACGGGAGCCCCCCCACCAAAAAGCGGGGTGGGCCCACCGAGCTGCGGAGCGATCAAccccttgtggtaa > 1:559080/1‑140 (MQ=255) cATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATa < 2:740306/149‑1 (MQ=255) cATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAAATCACACACGGTAGCCCCCACATCAGAAAGCGTGGGGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATaa > 2:20841/1‑150 (MQ=255) aTAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATCACAAACGGGAGCCCCCACACCAAAAAAAGTGGGGGGGACAACCAGCTGCGTAGCGGTCATCCCTTTCTGATaa > 1:113670/1‑149 (MQ=255) aTAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAAc < 1:111553/151‑1 (MQ=255) gggTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAg < 2:622037/151‑1 (MQ=255) tGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGAAGCGCGCGGGAAAAATTAAACACGGGAACCCCCCCATCAGAAAACGGGGGGCGGACACCCAGCTTCGTAAGGATCATCCCCTTCTGATAAAACAACACAGGcacc > 1:579306/1‑151 (MQ=255) cAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGGGCGGGAAAAATAACACACCGGAAACCCCACCCCAGAAAGAGTGGGGGGCACACCGGGCTTCGGAGGGGTCATCCCCTGCTGGTAAAACAACACATGcaccac > 1:32561/1‑151 (MQ=255) ccGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATTACACACCGGAGCCCCCACACCACAAAACGGGGGGGGCACACCCAACTGCGGAGCGGTCATCCCCTTCTGATAAACCAGCAACGGCACCCCtt > 1:894003/1‑149 (MQ=255) gaggaAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTAt < 2:34924/151‑1 (MQ=255) ggaAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGTAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAGCCAGCACTGGCACCACTTCGCGGGTAtg < 1:990420/150‑1 (MQ=255) aagaaaagaGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTAtggtgg < 2:290818/151‑1 (MQ=255) aaaagaGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATCACACACGGTAAACCCCCCACCAAAAAACGCGGGGGGCACACCGAGCCGCGTGGCGGTCATCCCCTTCTGATAAAACAACACTGGCACCCCCTCCCCGGGAtggggg > 1:717893/1‑148 (MQ=255) gagCGACCATTTTGTGGCGGTGGCGGGCGGGAAAAATTACACACGGTAGCCCCCCCATCAGAAAACGGGGGGCGCACACCGAGCTGCGGAGCGATCATCCCCTTCTGATAAAACAACAACGGCCCCACCTCGCGGGGAtggtgg > 2:719269/1‑144 (MQ=255) aTTTTGTGGCGGTGGCGCGCGGCAAAAATCACACAACGTAAACCCCCCATCCAAAAGCGGGGGGCGCACACCCAGACGCGCAGCGGCCATCCCCTGCTGATAAACCCACACCCGCACCACTTCGCGGGGATGGGGGCTGTGACCAACgg > 1:802682/1‑149 (MQ=255) tttGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCg < 2:447334/150‑1 (MQ=255) gtggcggtggcgCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATc < 2:235995/151‑1 (MQ=255) tggcggtggcgCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCg > 2:535805/1‑151 (MQ=255) gcggtggcgCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGtt < 1:732862/151‑1 (MQ=255) gcgGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATcc < 2:608141/151‑1 (MQ=255) ggAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGc < 1:1000617/151‑1 (MQ=255) gAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGcc > 1:986148/1‑151 (MQ=255) | GTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCC > NC_000913/3508519‑3508806 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |