Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,287,7100GA43.8% 55.2 / 41.6 64intergenic (‑339/‑54)acs/nrfAacetyl‑CoA synthetase/nitrite reductase, formate‑dependent, cytochrome
Reads supporting (aligned to +/- strand):  ref base G (28/8);  new base A (0/28);  total (28/36)
Fisher's exact test for biased strand distribution p-value = 4.82e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCT  >  NC_000913/4287560‑4287853
                                                                                                                                                      |                                                                                                                                               
atgcTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTg                                                                                                                                                 >  2:743973/1‑151 (MQ=255)
 tgcTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTg                                                                                                                                                 >  1:1037245/1‑150 (MQ=255)
  gcTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACAGTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGa                                                                                                                                                <  1:880414/150‑1 (MQ=255)
     gTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGaaa                                                                                                                                              <  1:475514/149‑1 (MQ=255)
             agagGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATaaa                                                                                                                                     >  1:1047880/1‑150 (MQ=255)
                ggAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAAcc                                                                                                                                 <  1:284150/151‑1 (MQ=255)
                  aaGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGc                                                                                                                               >  1:816783/1‑151 (MQ=255)
                       aCTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTg                                                                                                                          >  2:71416/1‑151 (MQ=255)
                          gACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGca                                                                                                                        >  2:99703/1‑150 (MQ=255)
                          gACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGca                                                                                                                        >  1:223704/1‑150 (MQ=255)
                           aCTAACTCAAAAGTGGTATTTTACATGCATTAACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAAAAATGTCATGCCAGTGTGGGTGCGGTTACTGTCGTAAAAAACAAAAAAAACCGCCATTGcaac                                                                                                                      <  1:769744/151‑1 (MQ=255)
                              aaCTCAAAAGTGTTTTTTTACATGCTCTTAAAATTGATAAAAGACAACATTCACAGTGGGGTTATTTGTTACACAGAGGGGCGAAAAATGTCATCCCAGTTTAGGTGCGTTTACTCTCGTAAAAAACAATAAAAACCGCCATTGCAACAAt                                                                                                                   <  1:1008237/151‑1 (MQ=255)
                               aCTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATg                                                                                                                  >  1:183284/1‑151 (MQ=255)
                                 tctAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGgc                                                                                                                >  1:967127/1‑151 (MQ=255)
                                 tctAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGgc                                                                                                                >  1:557000/1‑151 (MQ=255)
                                    aaaGTGGTTTTTTACTTGCACTTACAATTGATAAAAGAAAACATCCACAGTGTGGTTTTTTGTTACACATGGGGGCGAGAAATGTCAGGACAGTGTAGGTGCGGTTACTGTCTTAAAAAACAAAAAAAACCGCCATTGCAACAATGgcgc                                                                                                              <  1:158327/150‑1 (MQ=255)
                                    aaaGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGgcgc                                                                                                              >  1:706159/1‑150 (MQ=255)
                                    aaaGTGGTATTTTACATGCACTTACAATTGATAAAAGACAACATTCACGGTGTGGTTATTTGTTACACATGGGGGCAAGCAATGTCATGACAGTGTGGGGGCGTTTACTGTCGTAAAAAACAAAAAAAACCGCCATTGCAACAATgg                                                                                                                 <  1:865547/147‑1 (MQ=255)
                                           tATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGTTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCgg                                                                                                       <  2:563995/150‑1 (MQ=255)
                                           tATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCgg                                                                                                       >  1:1002927/1‑150 (MQ=255)
                                           tATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCgg                                                                                                       >  1:965229/1‑150 (MQ=255)
                                              tttACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATg                                                                                                    >  1:731266/1‑150 (MQ=255)
                                               ttACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGa                                                                                                   >  2:309372/1‑150 (MQ=255)
                                                tACATGCATTTACAATTGATTAAAGACAACATTCACAGTGTGGTTTTTTTTTACACATGGGGGCGAGAAATGTCATGACAGTGTAGGTGCGTTTACTGTCGGGAAAAACAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGaa                                                                                                  <  2:377018/150‑1 (MQ=255)
                                                 aCATGCATTTACAATTAATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTAATGACAGTGTAGGTGCGTTTCCTGTCGTGAAAAACAATAAAAGCCGCCATTGCAACAATGGCGCAATTCGGATGaa                                                                                                  <  1:454818/149‑1 (MQ=255)
                                                       aCTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTa                                                                                           >  1:1043065/1‑150 (MQ=255)
                                                       aCTTACAATTGATAAAAGACAACATTCACAGTGTGGTTATTTGTAACACATAGGGGCGACCATTGTCATGACAGTTTAGGTGCGGTTACTGTGGAAAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTAt                                                                                          <  2:968452/151‑1 (MQ=255)
                                                         ttAAAATTGATAAAAGAAAACATTCACAGTGTGTTTTTTTGTAACACATAGGGGCGAGAAATGTCATGACAGTGTAGGTGCGGTTCCTGTCGTAAAAAACAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTa                                                                                           <  1:20611/148‑1 (MQ=255)
                                                               ttGATAAAAAAAAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGAAATGTAATGACAGGGTGGGTGCGGTTACTGTCGTGAAAAACAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGAc                                                                                       <  1:973366/146‑1 (MQ=255)
                                                                tGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGAt                                                                                 >  2:362124/1‑151 (MQ=255)
                                                                  ataaaaGACAACATTCACAGTGTGGTTATTTTTTACACATAGGGGCGAGAAATGTCATGACAGTGTAGGTGCGTTTACTGTCGTAAAAAACAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGAt                                                                                 <  1:345005/146‑1 (MQ=255)
                                                                  ataaaaGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGAAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTAAAAAACAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGAt                                                                                 <  1:167408/146‑1 (MQ=255)
                                                                       aGAAAACATCCACAGGGTGGTTTTTTGTTACACATGGGGGCGAGCAATGTCATGAAAGTGTGGGTGCGTTTACTGTCGTAAAAAAAAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATa                                                                          <  1:259196/151‑1 (MQ=255)
                                                                         acaacaTTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATaa                                                                         >  2:663355/1‑150 (MQ=255)
                                                                         acaacaTTCACAGTATGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATa                                                                          >  2:943128/1‑149 (MQ=255)
                                                                         acaacaTTAACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATaa                                                                         >  1:541364/1‑150 (MQ=255)
                                                                              atgcacaGTGGGTTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGAAAAAAACAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAacgc                                                                     <  2:825799/146‑1 (MQ=255)
                                                                               ttCACAGTGTGGTTATTTGTTACACATAGGGGCGAGAAATGTCAGGAAAGTGTAGGTGCGGTTACTGTCTTAAAAAACAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAacgca                                                                    <  1:324387/149‑1 (MQ=255)
                                                                                tCACAGTTTGGTTATTTTTTACAAATAGGGGCGAGAAATGTCATAACAGTGTAGGTGGGGTTACTGTCGTGAAAAAAAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAacgcac                                                                   <  2:670510/149‑1 (MQ=255)
                                                                                      tgtgTTTATTTTTTACACATAGGGGCGAGCAATGTCATGACAGTGTGGGTGGGTTTACTGTCGTAAAAAAAAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTAt                                                            <  2:44690/150‑1 (MQ=255)
                                                                                          gTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTc                                                        >  1:394864/1‑150 (MQ=255)
                                                                                                ttttACACAGGGGGGCGAGAAATGTAATGACAGTGTGGGGGGGTTTACTGTCGTAAAAAAAAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTa                                                  <  2:967160/148‑1 (MQ=255)
                                                                                                tGTAACACAGAGGGGCGAGAAGTTCCATCAGGGTGTGGGTGGGGTTACTGTCGAAAAAAACAAAAAAACCCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTa                                                  <  2:1037286/150‑1 (MQ=255)
                                                                                                  ttACAAATGGGGGCGAGAAATTTCATGACAGTGTGGGTGCGTTTACTGTCGTAAAAAAAAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTa                                                  <  2:428700/148‑1 (MQ=255)
                                                                                                    acacATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGAt                                             >  1:454035/1‑151 (MQ=255)
                                                                                                      acaTAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTc                                           >  1:309281/1‑151 (MQ=255)
                                                                                                        aTAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCt                                         >  1:807015/1‑151 (MQ=255)
                                                                                                         tAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCtt                                        >  2:995455/1‑151 (MQ=255)
                                                                                                           ggggCGGGCAATGTAAGGCCGGTAATGGGGCGGTTTTTTTCGGAAAAAACAAAAAAACCCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTAGGACAAGGATAAAAATAAGCGCACGCCGTATCTTCAGCTTATTGATTCCtttt                                      <  1:58318/151‑1 (MQ=255)
                                                                                                           ggggCGAAAAATTTCATGACAGTGTGGGTGCGTTTGCTGTCGAAAAAAAAAAAAAAACCCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCtttt                                      <  1:338020/151‑1 (MQ=255)
                                                                                                           gggcggaccattGTCATGTTAGTGAAGGGGGGTTTACTTTCGTAAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGAtt                                            <  2:453212/134‑1 (MQ=255)
                                                                                                                gAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTtttttctt                                 >  1:216779/1‑151 (MQ=255)
                                                                                                                    attttaaggagaGTGTGGGTGCGTTTACTGTAGAAAAAAAAAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAACCGCACGCCGTATCTTCAGCTTATTGATTCCTtttttctttt                               <  1:930548/138‑1 (MQ=255)
                                                                                                                    attGTCAGGACAGTGTGGGTGGGTTTACTGTCGAAAAAAAAAAAAAAACCCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTtttttct                                  <  1:912817/144‑1 (MQ=255)
                                                                                                                    aaTGTCATTACGGTGTGGGGGGGTTTACTCCCTAAAAAAAAAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTAGGACAAGGATAAAAATAACCGCACGCCGTATCTTCAGCTTATTGATTCCTtttttctttttc                             <  2:612659/151‑1 (MQ=255)
                                                                                                                    aaTGTAATGACGTTGTGGGTGGGTTTCCTGTCGTAAAAAACAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTtttttcttttt                              <  2:937980/150‑1 (MQ=255)
                                                                                                                      tGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCAc                           >  1:57490/1‑151 (MQ=255)
                                                                                                                       gTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACt                          >  2:402919/1‑151 (MQ=255)
                                                                                                                          aTGACAGTGTGGGTGCGTTTACGGTCGAAAAAAACAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTc                        <  2:790036/150‑1 (MQ=255)
                                                                                                                             acggtgtAGGTGGGTTTCCTGGCGTAAAAAAAAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGtt                    <  2:397500/148‑1 (MQ=255)
                                                                                                                                 tgggggTGGGGTTTCTGTCGAAAAAAAAAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCa                            <  1:859355/135‑1 (MQ=255)
                                                                                                                                   tAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCt              >  2:147117/1‑151 (MQ=255)
                                                                                                                                     ggggggtttATTGTCGTAAAAAAAAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACtt                         <  2:982971/131‑1 (MQ=255)
                                                                                                                                        gCGTTTACTGTGGTAAAAAAAAAAAAAACCCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACa         <  1:663334/151‑1 (MQ=255)
                                                                                                                                          gtttACTTTGGTAAAAACAAAAAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACaaa       <  2:72776/149‑1 (MQ=255)
                                                                                                                                               cTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGgct  >  1:127975/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                               
ATGCTGTTGAAAAAGAGGAAGATACTGACTAACTCTAAAGTGGTATTTTACATGCACTTACAATTGATTAAAGACAACATTCACAGTGTGGTTATTTGTTACACATAGGGGCGAGCAATGTCATGACAGTGTAGGTGCGGTTACTGTCGTGAAAAACAATAAAAACCGCCATTGCAACAATGGCGCAATTCGGATGAAGCCCCTATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTTTTCACTTCTGTTCACGCTGAACAAACGGCT  >  NC_000913/4287560‑4287853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: