| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,457,105 | 0 | A | G | 47.4% | 51.2 / 31.7 | 57 | I441V (ATC→GTC) | mpl | UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase |
| Reads supporting (aligned to +/- strand): ref base A (4/26); new base G (27/0); total (31/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.11e-12 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGA‑TCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCG‑CAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTC > NC_000913/4456967‑4457255 | caggtggcagAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGA‑TCCATCAGAAAc < 2:207180/151‑1 (MQ=255) aggtggcagAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGGGGGG‑TCCATCAGaaa > 2:200661/1‑149 (MQ=255) gtggcagaagtggcagaaGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTTGGGGGG‑TCCATCCGAAAatgc > 2:1014390/1‑151 (MQ=255) gcagaagtggcagaagCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGA‑TCCATCAGAAACTGCTgg < 2:888898/151‑1 (MQ=255) agaagtggcagaagCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGGGGGG‑TCCATCAGAAAATGCTgg > 1:1028343/1‑149 (MQ=255) agaagtggcagaagCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑GTTTGGGGGGG‑TTCATCCGaaaacgct > 2:973235/1‑142 (MQ=255) agtggcagaagCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGA‑TCCATCAGAAACTGCTGGATggg < 1:636357/151‑1 (MQ=255) agtggcagaagCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑GTTTTGGGGGG‑GCCCTCAAAAAAAGCTggggggg > 1:577758/1‑146 (MQ=255) agtggcagaagCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑GTTTGGGGGGG‑GCCATTAGAAAAACCtgggtggg > 1:913009/1‑151 (MQ=255) ggcagaagCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGA‑TCCATCAGAAACTGCTGGATGGGCTg < 1:158462/151‑1 (MQ=255) cagaagCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTTGGGGGG‑TCCATCAGAAAATGCTGGAAGGGCTgg > 2:708405/1‑150 (MQ=255) agaagCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGA‑TCCATCAGAAACTGCTGGATGGGCTGGCg < 1:756343/151‑1 (MQ=255) agCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑GTTTTGTGGGG‑GCCATCAGAAACTGCtgggtggggcgggg > 1:533849/1‑142 (MQ=255) ccTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGA‑TCCATCAGAAACTGCTGGATGGGCTGGCgaagaa < 1:262639/151‑1 (MQ=255) cAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGTTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGA‑TCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGaa < 1:529889/150‑1 (MQ=255) cTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGG‑TCCATCAGAAAATGCTGGATGGGGTGGGGAAGAAAGCGGAAGcc > 2:156312/1‑149 (MQ=255) cTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑ATTTGGGGGGG‑TCCCCCAGAAAATGCTGGGTGGGGTGGGGAAGAAGGGGGGAGAcgc > 1:899485/1‑151 (MQ=255) gCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGA‑TCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCcgcg < 2:678980/150‑1 (MQ=255) gCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGA‑TCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCcgc < 2:390628/149‑1 (MQ=255) cacTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTTGTGGGG‑TCCATCAGAAAATGCTGGGTGGGGTGGCGGAGAAGGGGGGAGCcgcg > 2:1041114/1‑147 (MQ=255) cacTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGG‑TCCATCAGAAACAGCTGGGTGGGGTGGGGGAGAAAGGGGGAGCcgccccg > 1:611434/1‑146 (MQ=255) cTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTTGGGGGG‑GTCATCCGAAACTGCTGGGTGGGGTGGCGGAGAAAGCGGGAggc > 1:779508/1‑140 (MQ=255) ggAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGG‑TTCATCAGAAACTGCTGGATGGGGTGGGGAAGAAGGCGGGAGCCGCG‑CAGGaaat > 2:234665/1‑149 (MQ=255) ggCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGA‑TCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCG‑CAGTAATTCGGcc < 2:855187/151‑1 (MQ=255) ggCGATGTGGATACGCTGGCAGATATGGTGATGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGG‑TCCCTCAGAAAATGCTGGGTGGGCTGGCGGAGAAGGCGGGAGCCGCG‑CAGGAATTCGGcc > 1:805121/1‑151 (MQ=255) aTGTGGATACGCTGGCAGATATGGTGGTGAATACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGTGA‑TCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCG‑CAGTAATTCGGCCTTAg < 1:417850/151‑1 (MQ=255) ggATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGG‑TCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAAGCGGGAGCCGCG‑CAGGAAATCGGCCTTAGc > 1:835391/1‑148 (MQ=255) ggATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGA‑TCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCG‑CAGTAATTCGGCCTTAGCCa < 1:363661/150‑1 (MQ=255) gCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGA‑TCCATCAGAAACTGCTTGATGGGCTGGCGAAGAAGGCGGAAGCCGCG‑CAGTAATTCGGCCTTAGCCAGAtata < 2:649244/150‑1 (MQ=255) tGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGATTTTGGGGGGG‑CCCA‑CCGAAAACGCTGGGTGGGGTGGGGGAGAAAGGGGAAACCGCG‑GAGGAAATCTGccctttgccg > 1:355324/1‑135 (MQ=255) ggCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGA‑TCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCG‑CAGTAATTCGGCCTTAGCCAGATATAGt < 1:994530/149‑1 (MQ=255) gCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGA‑TCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCG‑CAGTAATTCGGCCTTAGCCAGATATAGTATg < 2:1028384/151‑1 (MQ=255) gCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGA‑TCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCG‑CAGTAATTCGGCCTTAGCCAGATATAGTATg < 1:750943/151‑1 (MQ=255) cAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGG‑TCCATCAGAAACTGCTGGGTGGGGTGGCGGAGAAAGCGGGAGCCGCG‑CAGGAATTCAGCCTTAGCCAGGAATAATATg > 1:106090/1‑150 (MQ=255) aGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGA‑TCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCG‑CAGTAATTCGGCCTTAGCCAGATATAGTATGCg < 1:295414/151‑1 (MQ=255) gATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGG‑TCCCTCAGAAACTGCTGGATGGGGTGGCGGAGAAAGCGGAAGCCGCG‑CAGGAATTCGGCCTTAGCCCGat > 2:351154/1‑140 (MQ=255) tatGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑GTTTGGGGGGG‑TCCATCAGCAACTGCTGGGTGGGGTGGCGAAGAAGGCGGAAGGCGCC‑CAGTAATTCAGACTTTACCACGTATTGTAttggt > 1:560043/1‑145 (MQ=255) atGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGA‑TCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCG‑CAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAgg < 2:355329/151‑1 (MQ=255) ggtggtGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGA‑TCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCG‑CAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGcc < 2:333662/151‑1 (MQ=255) gtggtgAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGG‑TCCACCAGAAAATGTGGGGTGGGCTGGGGGAGAAGGGGGGAGCCGCG‑CAGTAAATCGGGCCTAGCCCGAGATAGTATGCGGAg > 1:578257/1‑147 (MQ=255) aaaaCCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGA‑TCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCG‑CAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAg < 2:925485/151‑1 (MQ=255) gCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑CTTTGGTGGGG‑GCCATCAAAAAATGCTGGGTGGGGTGGGGAAGAAGGCGGGAGCCGCG‑CAGGAAATCGGCCCTAGCCAGATATAGTATGCGGAGGCCGGGTAAGGCggt > 1:530108/1‑148 (MQ=255) cAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGG‑ACCCTCAGAAACTGCTGGGTGGGCTGGCGAAGAAGGGGGAAGCCGCG‑CCGTAATTCGGCCCTAGCCAGATATAGTATGCGTAGGCCGGGTaaagaggt > 2:333068/1‑141 (MQ=255) cAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGA‑TCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCG‑CAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCAc < 2:345454/150‑1 (MQ=255) cAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGGGGGA‑TCCATCAGAAAATGGTGGATTGGCTGGCGAAGAAAGAGGAAGCCGCC‑CAGGAATTCGGCCCTAGCCAGATATAGTATTCGTAGGCCGGGTAAAGCGGTCAc > 2:299132/1‑150 (MQ=255) aGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGG‑GCCATCAGAAACTGCTGGGTGGGGGGGGGAAGAAAGGGGGAGGCGCG‑CAGGAAATCGGCCCTAGCCCGATATAGTATGCGTAGGCCGGGTAAGGCGGTCAc > 1:884499/1‑149 (MQ=255) ccATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGG‑TACATCACAAACTGCTGGATGGGGTGGGGGAGAAGGCGGGAGCCCCGCCGGGAATTGGGCCTT‑CCCAGCATTAAGATGCGTAGGCCCGATCAGGGCTTTACGCCCCATACGGc > 1:682586/1‑151 (MQ=255) cATATTCTGGTGATGAGCAACGGCGG‑TTTTTGTGGGACTTAATCTCAAACTG‑TCGGTGGGGCGGGGGAGAAAGGGGAAGCCGCC‑CAATAATTTTGCCTTTGTGCGACATAGAACGCGTATGCCCGGGAAAGTGTTCTCGCCGCACCCGGc > 1:803533/1‑150 (MQ=255) cATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGA‑TCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCG‑CAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa < 2:844939/151‑1 (MQ=255) tatTCTGGTGATGAGCAACGGCGG‑TTTTTGTGGGG‑GCCATCAGAAACTTCTGGGTGGGGTGGCGGAGAAGGCGGGAGCCGCG‑CAGGAAATTGGCCTTAGCCCGATATAGTATGCGTAGGCCGGGTAAGGCGGTCACGCCGCATCCGGCAtt > 1:920749/1‑151 (MQ=255) atTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGA‑TCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCG‑CAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAtt < 1:335984/150‑1 (MQ=255) tGGTGATGAGCAACGGCGG‑TTTTGGTGGGG‑TCCATCAGAAACTGCTGGGGGGGCTGGCGAAGAAGGGGGGAGCCGCG‑CAGGAATTTGGCCTTAGCCAGAGATAGTATGCGTAGGGCGGGTAAGGCGTTTACCCCCCATCCCGCAttt > 1:465899/1‑147 (MQ=255) gatgaGCAACGGCGG‑TTTTGGTGGGA‑TCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCG‑CAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAg < 2:770894/151‑1 (MQ=255) gCAACGGCGG‑TTTTGGTGGGA‑TCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCG‑CAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCt < 1:957559/151‑1 (MQ=255) ttGGTGGGA‑TCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCG‑CAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCtt > 2:1036255/1‑151 (MQ=255) gtggGA‑TCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCG‑CAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGAt > 1:225/1‑151 (MQ=255) tggGA‑TCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCG‑CAGTAATTCGGCCTTAGCCAGATATAGTTTGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGAt < 2:492244/150‑1 (MQ=255) a‑TCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCG‑CAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTc > 1:331157/1‑151 (MQ=255) | CAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGA‑TCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCG‑CAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTC > NC_000913/4456967‑4457255 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |