Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,214,770	A→C	E575A (GAA→GCA)	rpoD →	RNA polymerase, sigma 70 (sigma D) factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,214,770	0	A	C	100.0%	217.8 / NA	54	E575A (GAA→GCA)	rpoD	RNA polymerase, sigma 70 (sigma D) factor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (34/20);  total (34/20)

CACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTACGC  >  NC_000913/3214624‑3214914                                                                                                                                                   |                                                                                                                                                 caccCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTg                                                                                                                                              <  1:1023827/151‑1 (MQ=255)     cTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGt                                                                                                                                           >  2:1001716/1‑150 (MQ=255)         aGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCATGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAAc                                                                                                                                       >  1:374355/1‑150 (MQ=255)            tGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCCCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGtt                                                                                                                                   >  1:824500/1‑151 (MQ=255)             gCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGACCACCGACTACACGCTGGAAGCAGTGGGTAAACAGt                                                                                                                                    <  2:708976/149‑1 (MQ=255)              ccGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCg                                                                                                                                 >  1:170993/1‑151 (MQ=255)               cGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGa                                                                                                                                <  2:620935/151‑1 (MQ=255)               cGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGa                                                                                                                                <  1:334662/151‑1 (MQ=255)                   ggATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGtt                                                                                                                            >  2:744736/1‑151 (MQ=255)                   ggATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGtt                                                                                                                            <  1:476652/151‑1 (MQ=255)                          gCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTAccc                                                                                                                        <  2:348354/148‑1 (MQ=255)                            gACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGaa                                                                                                                   <  2:143514/151‑1 (MQ=255)                             accaccGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGACCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAAc                                                                                                                  <  1:330251/151‑1 (MQ=255)                               caccGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGt                                                                                                                >  1:632929/1‑151 (MQ=255)                                accGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTa                                                                                                               <  2:92542/151‑1 (MQ=255)                                     aaGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGGACGTATCCGt                                                                                                          <  1:249546/151‑1 (MQ=255)                                     aaGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCg                                                                                                           >  2:921669/1‑150 (MQ=255)                                       gCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCa                                                                                                        <  2:586400/151‑1 (MQ=255)                                                cgGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATcgaagc                                                                                               >  2:1005754/1‑151 (MQ=255)                                                    aaCGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATcgaagcga                                                                                             >  2:381630/1‑149 (MQ=255)                                                           gacgTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTgc                                                                                    >  1:321375/1‑151 (MQ=255)                                                              gTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTgcgc                                                                                  >  1:87113/1‑150 (MQ=255)                                                                      tggcCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGt                                                                         >  1:516196/1‑151 (MQ=255)                                                                       ggcCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTc                                                                        <  2:609624/151‑1 (MQ=255)                                                                         ccTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCAc                                                                      <  2:497379/151‑1 (MQ=255)                                                                            gACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCg                                                                   <  2:180728/151‑1 (MQ=255)                                                                                    gTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTc                                                           >  1:629621/1‑151 (MQ=255)                                                                                     tGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCt                                                          >  1:262261/1‑151 (MQ=255)                                                                                       aaGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGa                                                        <  1:258588/151‑1 (MQ=255)                                                                                         gCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGa                                                        >  2:90477/1‑149 (MQ=255)                                                                                         gCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGa                                                        >  2:509114/1‑149 (MQ=255)                                                                                         gCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGa                                                        >  2:334573/1‑149 (MQ=255)                                                                                           aaaaGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGtg                                                    >  1:902236/1‑151 (MQ=255)                                                                                            aaaGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACGCGAGCCGTTCTGAAGtg                                                    >  1:182553/1‑150 (MQ=255)                                                                                                ttCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCg                                               >  2:1020923/1‑151 (MQ=255)                                                                                                 tCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGt                                              >  2:421350/1‑151 (MQ=255)                                                                                                 tCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGt                                              >  1:1047944/1‑151 (MQ=255)                                                                                                 tCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGt                                              >  1:266431/1‑151 (MQ=255)                                                                                                        aTGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTcc                                       >  2:609865/1‑151 (MQ=255)                                                                                                            gTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTgg                                    >  2:198235/1‑150 (MQ=255)                                                                                                            gTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTgg                                    >  1:995035/1‑150 (MQ=255)                                                                                                                 ggTATCGATATGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGAtt                              <  2:282933/151‑1 (MQ=255)                                                                                                                         tatGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGt                       >  2:318814/1‑150 (MQ=255)                                                                                                                         tatGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGt                       >  2:979340/1‑150 (MQ=255)                                                                                                                           tGAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAgg                    >  1:411411/1‑151 (MQ=255)                                                                                                                            gAACACCGACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAgg                    <  2:58635/150‑1 (MQ=255)                                                                                                                                   gACTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATc             >  1:124454/1‑150 (MQ=255)                                                                                                                                    aCTACACGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCa            <  2:243585/150‑1 (MQ=255)                                                                                                                                       acacGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAgg          <  1:744401/149‑1 (MQ=255)                                                                                                                                       acacGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCg        >  1:561808/1‑151 (MQ=255)                                                                                                                                          cGCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGtt      >  1:189769/1‑150 (MQ=255)                                                                                                                                           gCTGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTAc    >  2:100568/1‑151 (MQ=255)                                                                                                                                             tGGAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTAcgc  >  1:100937/1‑151 (MQ=255)                                                                                                                                              ggAAGCAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTAcg   <  1:859370/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                 CACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTACGC  >  NC_000913/3214624‑3214914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑