Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,097,581	0	A	G	42.3%	141.8 / 26.8	78	noncoding (72/88 nt)	serX	tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (10/35);  new base G (33/0);  total (43/35)
Fisher's exact test for biased strand distribution p-value = 1.80e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAA  >  NC_000913/1097445‑1097729                                                                                                                                          |                                                                                                                                                      cACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGAt                                                                                                                                         <  2:918946/151‑1 (MQ=38)   cGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATAc                                                                                                                                       <  2:434659/151‑1 (MQ=39)      tCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTCGAACCCCCCCTACGt                                                                                                                                     >  2:569630/1‑150 (MQ=255)      tCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGA‑GGGGGGGGTTTGGAcccccccaacgtt                                                                                                                                    >  2:88886/1‑143 (MQ=11)        tGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTCGAAccccccccacgtt                                                                                                                                    >  2:1283946/1‑141 (MQ=255)         gTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGtt                                                                                                                                    <  2:417543/148‑1 (MQ=255)           ccAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGCGA‑GGGGGGGGGTCGGAACCCCCCCACGTTGcc                                                                                                                                 >  2:1015568/1‑149 (MQ=11)            cAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTTGAAacccccctaccgt                                                                                                                                    >  2:1266327/1‑137 (MQ=1)             aaCTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGtat                                                                                                                             <  2:868828/151‑1 (MQ=255)              aCTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGtata                                                                                                                            <  2:1206965/151‑1 (MQ=255)              aCTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGtata                                                                                                                            <  2:550325/151‑1 (MQ=255)                  gCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATacac                                                                                                                         <  2:681251/150‑1 (MQ=255)                         ccAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCa                                                                                                                <  1:64348/151‑1 (MQ=255)                              tGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTTGAAACCCCCCTAAGTTGCCGGTTacacaactt                                                                                                                    >  1:957807/1‑139 (MQ=11)                                   aaCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTCGAAACCCCCGTAAATTGGCGGAAACACA‑CATTTCAggggggggcc                                                                                                      >  1:575580/1‑143 (MQ=11)                                      cccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGG‑GGGGGGGGGTCGAAACCCCCCTACGTTTCCGTATACACA‑CTTTCCCGGCGGGCTccct                                                                                                    >  2:988120/1‑148 (MQ=17)                                      cccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTCGAACCCCCCATACGTTGCCGGGTACAAA‑CTTTTCAAGCGTGCGccct                                                                                                    >  2:817314/1‑148 (MQ=14)                                       ccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGG‑GGGGGGGGGTTGGACCCCCCATACGGTTCCCGATACACA‑CCTTTCCGGCGGGCTcc                                                                                                      >  2:1433633/1‑147 (MQ=11)                                         ggTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGc                                                                                                <  1:451817/151‑1 (MQ=255)                                           tCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGG‑GGGGGGGGGTTGAACCCCCCCCACGTTTCCCGATACACA‑CCTTTCAGGCGTGCTCCCTCAGcc                                                                                               >  2:1006488/1‑150 (MQ=14)                                            cGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTTGAAACCCCGATAAGGTTCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGcc                                                                                               >  1:463891/1‑149 (MQ=18)                                              gggCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTc                                                                                           <  1:1266361/151‑1 (MQ=255)                                                gCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCgg                                                                                         <  1:562011/151‑1 (MQ=255)                                                gCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGA‑GGGGGGGGGTTTGACCCCCCACACGTTGCCGTATAAACA‑CTTTTCCGGCGGGGTCCCTcctcccccccg                                                                                         >  2:986038/1‑141 (MQ=14)                                                  ttCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTCGGAACCCCCCTACCGTGCCCTATAAACA‑CTTTTCCAGCGCGCCCCtttctcccc                                                                                             >  1:563068/1‑138 (MQ=11)                                                                   tgtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGG‑GGGGGGGGGTCGGACCCCCCATACCTTGCCGTATACACA‑CATTTTAGGCGGGGCCCTTTAGCCCCTCGGGCACCTCACCaaaattt                                                                        >  1:755271/1‑145 (MQ=14)                                                                   tgtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTTGAAACCCCCACACGTTTCCCGATAAAAA‑CTTTTCAAGGGGGGTCCTTCCGCCCCTCCGGCAccccccccaaattt                                                                        >  2:802889/1‑137 (MQ=2)                                                                     tgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGA‑AGGGGGGGGTCGAAACCCCCAAACGGTTCCGTTTAAACA‑CCTTTCATGGGGGCCCCCTCTGCCACACAGGCACCCCACCCAAATTTCggt                                                                    >  1:373604/1‑149 (MQ=2)                                                                     tgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAGA‑GGGGGGGGGTTGAACCCCCCCTACGTTGCCGTATAAAAA‑CTTTCCAGGGGGGCTCCTTCAGACACCCGGACACCTCACCAAAATTTCGt                                                                     >  2:384656/1‑150 (MQ=14)                                                                       cAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTcc                                                                  <  2:1066273/151‑1 (MQ=255)                                                                       cAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAGA‑GGGGGGGGGTTGAAACCCCCATAAGGTTCCCGATACACC‑CTTTTCCCGCGGGGGCCTTCAACCCCCCCGACACCCCCCCCAAATATCGtt                                                                    >  2:780662/1‑149 (MQ=2)                                                                            taCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTTGAACCCCCCCTACGTTGCCGTATACAAA‑CTTTCCAGGCGTGGTCCTTCAGCCACCCGGACACCCCACCCAAATTTTGTTCCTGt                                                               >  1:1308988/1‑149 (MQ=14)                                                                              cGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTg                                                           <  2:492464/151‑1 (MQ=255)                                                                              cGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTg                                                           <  2:1120768/151‑1 (MQ=255)                                                                                 aaaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTg                                                        <  1:505481/151‑1 (MQ=255)                                                                                   aaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGa                                                      <  1:1024486/151‑1 (MQ=255)                                                                                    aaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTCGAACCCCCCCTACGTTTCCGTATACACACCTTTCCA‑GCGGGCTCCTTTCGCCACCCCGGCACCCCACCAAAATTTTGTTCCTGTCtt                                                            >  1:1371885/1‑144 (MQ=14)                                                                                    aaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTCGAACCCCCCATACCGTTCCCTATAAACA‑CTTTCCAGGCGTGCTCCCTCAGCCACTCGGACACCCCCCCCAAATTTCGTTCCTGTCtt                                                            >  1:780035/1‑144 (MQ=14)                                                                                     aaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTCGAACCCCCGATACGTTGCCGTTTACACA‑CTTTTCAGGCGTGCTTCTTCAGCCACCCGGACACCTCAACCAATTTTCGGttctttctttctcg                                                       >  1:617432/1‑146 (MQ=11)                                                                                     aaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAAc                                                    <  1:174138/151‑1 (MQ=255)                                                                                      aaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTCGAAACCCCCATACGTTGTCGGCTACAAA‑CTTTTCAGGCGGGGCCCCTCAGCCCCCCGGACACCCCACCCAAATTTTGTTTCTGTCTTTCTgggaa                                                    >  1:1211590/1‑147 (MQ=9)                                                                                       aGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTTTGACCCCCCCTACCTTTCCGTATAAACA‑CTTTTCAGGCGGGGTCCCTCAGCCACCCCGACACCCCCCCCAATTTTTGTTTCTGTCtttttggga                                                     >  2:1125486/1‑141 (MQ=2)                                                                                         cccGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGgc                                                <  1:6418/151‑1 (MQ=255)                                                                                          ccGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTCGAACCCCCCCTACGTTTCCGGATAAAAA‑CCTTTCAGGCGGGCTCCCTCCGCCACCCCGACACCTCCCCCAAATTTCGTTCCTTTCTTTCTGGGACggg                                                 >  1:56020/1‑149 (MQ=9)                                                                                              aCTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTa                                            <  1:29031/150‑1 (MQ=255)                                                                                                 ttCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAAtt                                         <  2:910195/150‑1 (MQ=255)                                                                                                  tCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTTCGAACCCCC‑CTAAGTTTCCGTATATACC‑CCTTTCAGGCGCGGGCCTTCCGCCCCCCTGGGACCCCACCAAACTTTCGGTCCTGTCTTTCTGGAACggggg                                               >  2:665740/1‑141 (MQ=9)                                                                                                  tCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCt                                             <  1:665750/145‑1 (MQ=255)                                                                                                  tCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAAttt                                        <  2:942299/150‑1 (MQ=255)                                                                                                  tCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAAttt                                        <  1:512269/150‑1 (MQ=255)                                                                                                  tCGTACGAGCTCTTCTTTAAATATGGCGGAGA‑GGGGGGGGATTGAACCCCCCGTACGTTGCCCTATACACA‑CTTTTCCCGCGGGCTCCCTTCGCCACCCCGGCACCCCCCCCAATTTTTGGTCCTCTTTTTCTGGAACGGgcgccca                                           >  1:1423293/1‑144 (MQ=2)                                                                                                    gTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTCGAAACCCCCACACCTTGCCGTATACACA‑CTTTTCCGGGGGGGTCCCTCACCCACTCGGGCACCCCACCCAATTTTCGTTCCTGTCTTTCTGGGAAGGGGGCTAAttt                                        >  2:56230/1‑148 (MQ=9)                                                                                                    gTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAgg                                     <  1:1382954/151‑1 (MQ=255)                                                                                                       cGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGaa                                  <  2:460775/151‑1 (MQ=255)                                                                                                       cGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGaa                                  <  1:1433676/151‑1 (MQ=255)                                                                                                        gAGCTCTTCTTTAAATATGGCGGGGA‑GGGGGGGGGTTGAACACCCGATTCGTTGCCGTATATACA‑CTTTTCAGGCGGGCTCCTTTCCCCACCCGGACACCCCACCCCATTATCGTTCCTTTCTTTCTTGAACGGGCGCTAATTTTggg                                    >  2:1143795/1‑148 (MQ=9)                                                                                                            tcttctTTAAATATGGCGGTGA‑GGGGGGGGTTTGAACCCCCCCTACGTTTCCCTATACACA‑CCTTTCCGGGGGGCTCCTTCCGCCCCCCGGACACCCCACCCAATTTTCGTTCCTGTCTTTCTGGGACGGGGGCCAATTTAGGGgaa                                 >  1:701429/1‑147 (MQ=12)                                                                                                               tcttTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATg                            <  1:100707/149‑1 (MQ=255)                                                                                                               tcttTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATg                            <  1:569638/149‑1 (MQ=255)                                                                                                                  ttAAATATGGCGGTGA‑GGGGGGGGTTCGGACCCCCCCCAAGGTTCCCTATAAAAA‑CTTTCCCGGCGGGGTTCCTCCGCCACCCGGACACCCCCCCCAAATTTCGTTCCTGTCTTGCTGGGAAGGGGGCCAATTTTTGGGAATTATg                            >  2:1272954/1‑146 (MQ=9)                                                                                                                    aaaTATGGCGGTGA‑GGGGGGGGTTCGAAACCCCGATACGTTGCCGTAAACACC‑CTTTTCCGGGGGGCTCCTTCCCCCCCTCCGACACCCCCCCCAATTTTCGTTTCTGTTTTTTTGGAACGGGGGCCTATTTTTGGGAATCATGAcc                         >  2:333875/1‑147 (MQ=9)                                                                                                                    aaaTATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAg                     <  2:651809/151‑1 (MQ=255)                                                                                                                        atGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTc                  <  1:881160/150‑1 (MQ=255)                                                                                                                          ggCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTcaac               >  2:1360522/1‑151 (MQ=255)                                                                                                                            cGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTcaaca              >  2:50560/1‑150 (MQ=255)                                                                                                                            cGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTcaaca              >  1:506108/1‑150 (MQ=255)                                                                                                                             ggTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTcaacaa             >  1:361267/1‑150 (MQ=255)                                                                                                                             ggTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTcaacaa             >  1:382551/1‑150 (MQ=255)                                                                                                                             ggTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTcaacaa             >  2:1033234/1‑150 (MQ=255)                                                                                                                              gTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAAc           <  2:957784/151‑1 (MQ=255)                                                                                                                                gA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACtt         <  2:223827/151‑1 (MQ=255)                                                                                                                                gA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACtt         <  2:975213/151‑1 (MQ=255)                                                                                                                                 a‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACttt        <  1:1424572/151‑1 (MQ=255)                                                                                                                                   gggggggATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACtttt       >  1:1287983/1‑151 (MQ=255)                                                                                                                                    ggggggATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACttttt      >  2:907106/1‑151 (MQ=255)                                                                                                                                      ggggATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTg     >  2:348383/1‑150 (MQ=255)                                                                                                                                      ggggATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTg     >  2:1338776/1‑150 (MQ=255)                                                                                                                                        ggATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGaaa  <  2:1432661/151‑1 (MQ=255)                                                                                                                                          |                                                                                                                                                      CACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAA  >  NC_000913/1097445‑1097729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑