Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	29,486	0	C	A	45.2%	145.5 / 55.2	93	intergenic (+291/‑165)	dapB/carA	dihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base C (41/10);  new base A (0/42);  total (41/52)
Fisher's exact test for biased strand distribution p-value = 3.53e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTG  >  NC_000913/29338‑29630                                                                                                                                                       |                                                                                                                                                  tttGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCcaca                                                                                                                                                  >  1:163967/1‑150 (MQ=255)  ttgttgTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACaa                                                                                                                                                 <  1:839084/150‑1 (MQ=255)   tgttgtTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACaaa                                                                                                                                                >  2:974647/1‑150 (MQ=255)    gttgttTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACaaaa                                                                                                                                               <  1:1072343/150‑1 (MQ=255)    gttgttTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACaaaa                                                                                                                                               <  1:543000/150‑1 (MQ=255)    gttgttTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAaat                                                                                                                                              <  1:827842/151‑1 (MQ=255)       gttTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAaataa                                                                                                                                            <  2:40690/150‑1 (MQ=255)       gttTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAACCACAAaataa                                                                                                                                            >  1:1310212/1‑150 (MQ=255)         tttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAatat                                                                                                                                         >  2:803878/1‑151 (MQ=255)          ttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAatat                                                                                                                                         <  2:1279144/150‑1 (MQ=255)           tAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAAtata                                                                                                                                        >  1:489876/1‑150 (MQ=255)           tAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAAtata                                                                                                                                        >  1:559004/1‑150 (MQ=255)             aTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATaaaa                                                                                                                                     <  1:800517/151‑1 (MQ=255)               gTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATaaaaa                                                                                                                                    >  2:944302/1‑150 (MQ=255)                tAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATaaaaaa                                                                                                                                   <  1:1081330/150‑1 (MQ=255)                 aaaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTTATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑Tc                                                                                                                                <  2:156741/151‑1 (MQ=255)                  aaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑Tcc                                                                                                                               >  1:565914/1‑151 (MQ=255)                   aTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑Tccc                                                                                                                              >  2:911334/1‑151 (MQ=255)                    ttttGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCg                                                                                                                             >  1:488670/1‑151 (MQ=255)                        gACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCAt                                                                                                                         >  1:339600/1‑151 (MQ=255)                         aCCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCAtt                                                                                                                        >  1:40361/1‑151 (MQ=255)                           cATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTa                                                                                                                       >  1:1053836/1‑150 (MQ=255)                                      cTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACtttt                                                                                                            >  1:322444/1‑150 (MQ=255)                                      cTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACtttt                                                                                                            >  1:165220/1‑150 (MQ=255)                                       ttttttCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACtttt                                                                                                            >  2:1389558/1‑149 (MQ=255)                                          tttCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAgcgc                                                                                                       >  1:9698/1‑151 (MQ=255)                                             cTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGccc                                                                                                     >  2:1190394/1‑150 (MQ=255)                                              tGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCat                                                                                                   >  1:727851/1‑151 (MQ=255)                                               gccgttttttATTTCATGCACTCTTCTTGCTGCGAAAGCGTTTCCC‑AAAAAGGATT‑AAATGTTTTTTTGGTCGCTTAATGCCTAAAAAACTTGCGTGACCCAAAAAAAAAAAAAAAAAA‑CCCCGCCATTAAGTTGACTTTTAGCGCCCat                                                                                                   <  2:488663/145‑1 (MQ=255)                                               gCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCat                                                                                                   >  2:623092/1‑150 (MQ=255)                                                 tctttttttttTCATGCATTTTTCTTGCTGCGCAGGCTTTTCCA‑AAAACAGTTT‑AGATGTTTTTTTTGTCGTTAAATGCCTGAAAAACTTGCATGACCCAAAAAAAAATAAAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCata                                                                                                  <  1:680565/146‑1 (MQ=255)                                                    ttttttttTAATGCATTTTTTTTGCGGCGCAAGCTTTTCCA‑AAAACGGTTT‑AAATAATTTTTTTGTCTTTAAATGCCTAAAAAACTTGAGTGGCCCAAAAAAAAAAAAAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATAtctc                                                                                              <  2:1100992/150‑1 (MQ=255)                                                    tttttATTTCATATATTCTTTTTCCTCCG‑AAGGCTTTTCC‑AAAACCGGTTAAGATATTCTTTTTTTAGTTAAATCCTTGAAAAACATCCAGGCCACAAAAAAGTAAATAAAAAT‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATAtct                                                                                               <  2:368639/149‑1 (MQ=255)                                                      tttattttatGCAATCTTTTTGCTGCGAAAGCTTTTTCC‑AGAAGAGTTT‑AGTTGTTCTTTTTGTCTTTAAGTGTCGGAAAAACAGTCAGGAGCCAAAAAATAATATAAAAAT‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCa                                                                                            <  1:689075/150‑1 (MQ=255)                                                       ttATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                             >  1:197029/1‑148 (MQ=255)                                                        tttttCATGCAATTTTCTTGCGGCGCAAGCTTTTCCA‑AAAACAGTTA‑AAAAAATTTTTTTGTCGTTTATTCCCTAAAAAACATGAATGAGCAAAAAAAAAATAAAAAAAA‑CCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGa                                                                                          <  2:395184/148‑1 (MQ=255)                                                          tttCATGCATTTTTCTTGCTGCGAAAGCTTTTCCA‑AAAAAAGTTT‑AGATTTTTTTTTTTTCGTTTAATCCCTGAAAAACTGGAATGGGCCAAAAAATAAAAAAAAAAT‑CCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAAt                                                                                        <  2:65991/150‑1 (MQ=255)                                                            tCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgcc                                                                                     >  2:1312575/1‑151 (MQ=255)                                                                    tcttctTGCTGCGAAAGCTTTTTCC‑AGAACAGGTT‑AGATGATTTTTTTGTCGCTTAATGCTTGTAAAACAGGCATGACCCAAAAAAAAATAAAAAAAA‑CCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGttt                                                                              <  1:609183/150‑1 (MQ=255)                                                                          tgctgcGCAGGCTTTTTCC‑AAAACAGTTT‑AGATGATTTTTTTTTCGTTTAATGCTTGAAAAACATGAATGAGCCAAAAAATAATAAAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGcc                                                                           <  1:811081/147‑1 (MQ=255)                                                                             tgcGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAAtt                                                                    >  2:427820/1‑151 (MQ=255)                                                                              gcgcAAGCTTTTCCA‑AAAACGGGTT‑AGATTATTTTTTTTTCGTTTAATCCCTAAAAAACATGAATGACCCAAAAAAAAATAAAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGaa                                                                       <  2:86411/147‑1 (MQ=255)                                                                              gcgcAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtc                                                                   >  2:618941/1‑151 (MQ=255)                                                                               cgcAGGCTTTTTCA‑AGAACAGGTT‑AGATGATTTTTTTGTCGCTTAATGCCTGAAAAACATGCATGAGCCAAAAAAAAAAAAAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAAt                                                                     <  1:1087943/148‑1 (MQ=255)                                                                               cgaaggctttttCA‑AGAAAAGGTT‑AAATGTTTTTTTTGTCGTTAATTCCCTAAAAAACTTGCATGACCCAAAAAAAAAAAAAAAAAC‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCa                                                                          <  2:239919/135‑1 (MQ=255)                                                                                gcAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgt                                                                 >  2:258341/1‑151 (MQ=255)                                                                                gcAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgt                                                                 >  1:201647/1‑151 (MQ=255)                                                                                 cAAGCTTTTCCA‑AGAACAGGTA‑AGATGTTTTTTTTGTCGTTAAATCCTTAAAAAACATGCATGAGCCAAAAAATAATAAAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgt                                                                 <  1:932722/150‑1 (MQ=255)                                                                                   aaccttttCCAAGAACAGTTA‑AGATAATTTTTTTGTCGCTTATTGCCTAAAAAACATGAATGCACAAAAAAAAAATAAAAAAAA‑CCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgtc                                                                <  2:727838/146‑1 (MQ=255)                                                                                   aGCGTTTTCA‑AAAAAAGGTT‑AGATGATTTTTTTGTCGTTTAATCCCTATAAAACATGAATGACCCAAAAAATAATAAAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgtcg                                                               <  1:132597/150‑1 (MQ=255)                                                                                    gCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGt                                                             >  1:1363346/1‑151 (MQ=255)                                                                                     cGTTTACA‑AGAATAGGTT‑AGATGTTTTTTTTGTCGCTAATTGCCTGAAAAACATGCATGACCAAAAAAAAAAAAAAAAAAC‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGt                                                             <  1:483463/150‑1 (MQ=255)                                                                                       ttttCA‑AGAACAGGTA‑AGATAATTTTTTTGTCGTTTAATGCCTGAAAAACATGCATGACCCAAAAAATAATAAAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCgg                                                              <  1:803899/147‑1 (MQ=255)                                                                                       tttgCC‑AAAACAGTTT‑AAATGTTTTTTTTTTCTTTTAAGGCTTAAAAAACGTGCATG‑GCCAAAAAAAAAAAAAAAAAAGCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgtc                                                                <  2:470066/145‑1 (MQ=255)                                                                                       tttcCC‑AAAAAAGGTT‑AGATGTTTTTTTTGTCGTTTATTGCCTGAAAAACATGCATGACCCAAAAAATAATAAAAAAAT‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTaa                                                           <  2:430791/150‑1 (MQ=255)                                                                                            c‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGc                                                         >  2:317731/1‑147 (MQ=255)                                                                                              agaagaGGTT‑ATATTATTTTTTTGTCTCTTAATGCCTAAAAAACTGGCAGGAGCCAAAAAATAATAAAAAAAA‑CCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAg                                                       <  2:1057588/148‑1 (MQ=255)                                                                                                   aGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAt                                               >  2:1059779/1‑151 (MQ=255)                                                                                                   aGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAt                                               >  1:949784/1‑151 (MQ=255)                                                                                                   aGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAt                                               >  1:287695/1‑151 (MQ=255)                                                                                                    ggTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAtt                                              >  1:1127501/1‑151 (MQ=255)                                                                                                     gTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTg                                             >  2:1458858/1‑151 (MQ=255)                                                                                                      tt‑AGATGATTTTTTTGTCGTTAAATGCCTGAAAAACATGCAGGACCCAAAAAATAATAAAAAAAA‑CCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAt                                               <  1:1389599/148‑1 (MQ=255)                                                                                                           atgatCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGACATTCGTCGGTAAGCAGATTTGCATTGAttt                                         >  2:1448520/1‑150 (MQ=255)                                                                                                            tgtttttttttTCTTTTAATGCCTTAAAAACTTGCATGACCCACAAAAAAATAAAAAAAC‑CCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTg                                             <  2:260963/140‑1 (MQ=255)                                                                                                             gatCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACg                                      >  2:74077/1‑151 (MQ=255)                                                                                                                cTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtc                                    >  2:618002/1‑150 (MQ=255)                                                                                                                cTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtc                                    >  2:276678/1‑150 (MQ=255)                                                                                                                  ttttttcttttAATGCATTAAAAACATTCGTGAACAAAAAAATAAAAAAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAtt                                          <  1:184223/133‑1 (MQ=255)                                                                                                                    ttGTCCTTTAATCCCTGAAAAACATGAATGAGCCAAAAAAAAAAAAAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcatca                                <  1:410966/150‑1 (MQ=255)                                                                                                                     tGTCGCTAAATGCCTATAAAACATGCATGGGCCAAAAAAAAATAAAAAAAA‑CCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTcatcat                               <  1:323144/150‑1 (MQ=255)                                                                                                                        cttttAATGCCTAAAAAACATGCATGACCCAAAAAAAAATAAAAAAAA‑CCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCAtt                              <  2:489869/145‑1 (MQ=255)                                                                                                                          cTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGa                          >  2:320405/1‑150 (MQ=255)                                                                                                                           ttAATGCCTGAAAAACATGCATGAGCCAAAAAAAAATAAAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGaa                         <  1:944325/150‑1 (MQ=255)                                                                                                                           ttAATCCCTAAAAAACAGGCAGGAGCCAAAAAATAATAAAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgtg                           <  1:441545/148‑1 (MQ=255)                                                                                                                             attccttgaaaaaCATCCATGAGCCAAAAAAAAATAAAAAAAA‑CCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGa                          <  2:1096825/138‑1 (MQ=255)                                                                                                                             aatccctaaaaaaCATGCATGAGCCAAAAAATAATAAAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAt                        <  2:489069/140‑1 (MQ=255)                                                                                                                             aaTGCCTGAAAAACATGCAGGAGCAAAAAAAAAATAAAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAt                        <  1:1458902/149‑1 (MQ=255)                                                                                                                               tGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAat                    >  2:1140790/1‑151 (MQ=255)                                                                                                                                    gTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATg                 >  1:919421/1‑149 (MQ=255)                                                                                                                                     aaaaaCATCCATGAGCAAAAAAAAAAAAAAAAAAA‑CCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTa                      <  2:1020094/142‑1 (MQ=255)                                                                                                                                      aaaaGATGCATATGCCAAAAAAAAATATAAAAAC‑GCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGTAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCaa              <  2:597058/150‑1 (MQ=255)                                                                                                                                      aaaaCATGCATGGCCCAAAAAATAAAAAAAAAAA‑CCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCa               <  1:722382/149‑1 (MQ=255)                                                                                                                                      aaaaCATGCATGAGCCAAAAAATAATAAAAAAAA‑CCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAatat                  <  2:163964/146‑1 (MQ=255)                                                                                                                                      aaaaCATGCATGACCAAAAAAAAAATAAAAAAAT‑CCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCa               <  2:636462/149‑1 (MQ=255)                                                                                                                                       aaccttgcatgAGCCAAAAAATAATAAAAAAAA‑CCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGc                <  1:1097856/142‑1 (MQ=255)                                                                                                                                       aaccatgcagcggCCAAAAAAAAATATAAAAAT‑ACCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCaa              <  1:634067/137‑1 (MQ=255)                                                                                                                                             gcaggAGCAAAAAAAAAATAAAAAAAA‑CCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg      <  1:811094/151‑1 (MQ=255)                                                                                                                                              catgACCCAAAAAATAATAAAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg      <  2:1348250/150‑1 (MQ=255)                                                                                                                                               atgACCCAAAAAAAAATAAAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg      <  1:974670/149‑1 (MQ=255)                                                                                                                                                ggacccAAAAAATAATAAAAAAAA‑CCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagt   <  1:1330260/147‑1 (MQ=255)                                                                                                                                                  aGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgtgtg  >  1:851021/1‑150 (MQ=255)                                                                                                                                                  aGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagtg  >  1:314126/1‑150 (MQ=255)                                                                                                                                                   cccAAAAAATAAAAAAAAAAC‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgag    <  1:177688/146‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                  TTTGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTG  >  NC_000913/29338‑29630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑