Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091329,4960TA48.4% 98.3 / 66.3 93intergenic (+301/‑155)dapB/carAdihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base T (40/8);  new base A (0/45);  total (40/53)
Fisher's exact test for biased strand distribution p-value = 1.14e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

TTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAGG  >  NC_000913/29346‑29645
                                                                                                                                                        |                                                                                                                                                      
tttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAatat                                                                                                                                                        >  2:803878/1‑151 (MQ=255)
 ttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAatat                                                                                                                                                        <  2:1279144/150‑1 (MQ=255)
  tAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAAtata                                                                                                                                                       >  1:559004/1‑150 (MQ=255)
  tAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAAtata                                                                                                                                                       >  1:489876/1‑150 (MQ=255)
    aTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATaaaa                                                                                                                                                    <  1:800517/151‑1 (MQ=255)
      gTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATaaaaa                                                                                                                                                   >  2:944302/1‑150 (MQ=255)
       tAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATaaaaaa                                                                                                                                                  <  1:1081330/150‑1 (MQ=255)
        aaaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTTATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑Tc                                                                                                                                               <  2:156741/151‑1 (MQ=255)
         aaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑Tcc                                                                                                                                              >  1:565914/1‑151 (MQ=255)
          aTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑Tccc                                                                                                                                             >  2:911334/1‑151 (MQ=255)
           ttttGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCg                                                                                                                                            >  1:488670/1‑151 (MQ=255)
               gACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCAt                                                                                                                                        >  1:339600/1‑151 (MQ=255)
                aCCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCAtt                                                                                                                                       >  1:40361/1‑151 (MQ=255)
                  cATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTa                                                                                                                                      >  1:1053836/1‑150 (MQ=255)
                             cTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACtttt                                                                                                                           >  1:165220/1‑150 (MQ=255)
                             cTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACtttt                                                                                                                           >  1:322444/1‑150 (MQ=255)
                              ttttttCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACtttt                                                                                                                           >  2:1389558/1‑149 (MQ=255)
                                 tttCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAgcgc                                                                                                                      >  1:9698/1‑151 (MQ=255)
                                    cTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGccc                                                                                                                    >  2:1190394/1‑150 (MQ=255)
                                     tGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCat                                                                                                                  >  1:727851/1‑151 (MQ=255)
                                      gccgttttttATTTCATGCACTCTTCTTGCTGCGAAAGCGTTTCCC‑AAAAAGGATT‑AAATGTTTTTTTGGTCGCTTAATGCCTAAAAAACTTGCGTGACCCAAAAAAAAAAAAAAAAAA‑CCCCGCCATTAAGTTGACTTTTAGCGCCCat                                                                                                                  <  2:488663/145‑1 (MQ=255)
                                      gCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCat                                                                                                                  >  2:623092/1‑150 (MQ=255)
                                        tctttttttttTCATGCATTTTTCTTGCTGCGCAGGCTTTTCCA‑AAAACAGTTT‑AGATGTTTTTTTTGTCGTTAAATGCCTGAAAAACTTGCATGACCCAAAAAAAAATAAAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCata                                                                                                                 <  1:680565/146‑1 (MQ=255)
                                           ttttttttTAATGCATTTTTTTTGCGGCGCAAGCTTTTCCA‑AAAACGGTTT‑AAATAATTTTTTTGTCTTTAAATGCCTAAAAAACTTGAGTGGCCCAAAAAAAAAAAAAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATAtctc                                                                                                             <  2:1100992/150‑1 (MQ=255)
                                           tttttATTTCATATATTCTTTTTCCTCCG‑AAGGCTTTTCC‑AAAACCGGTTAAGATATTCTTTTTTTAGTTAAATCCTTGAAAAACATCCAGGCCACAAAAAAGTAAATAAAAAT‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATAtct                                                                                                              <  2:368639/149‑1 (MQ=255)
                                             tttattttatGCAATCTTTTTGCTGCGAAAGCTTTTTCC‑AGAAGAGTTT‑AGTTGTTCTTTTTGTCTTTAAGTGTCGGAAAAACAGTCAGGAGCCAAAAAATAATATAAAAAT‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCa                                                                                                           <  1:689075/150‑1 (MQ=255)
                                              ttATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                            >  1:197029/1‑148 (MQ=255)
                                               tttttCATGCAATTTTCTTGCGGCGCAAGCTTTTCCA‑AAAACAGTTA‑AAAAAATTTTTTTGTCGTTTATTCCCTAAAAAACATGAATGAGCAAAAAAAAAATAAAAAAAA‑CCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGa                                                                                                         <  2:395184/148‑1 (MQ=255)
                                                 tttCATGCATTTTTCTTGCTGCGAAAGCTTTTCCA‑AAAAAAGTTT‑AGATTTTTTTTTTTTCGTTTAATCCCTGAAAAACTGGAATGGGCCAAAAAATAAAAAAAAAAT‑CCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAAt                                                                                                       <  2:65991/150‑1 (MQ=255)
                                                   tCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgcc                                                                                                    >  2:1312575/1‑151 (MQ=255)
                                                           tcttctTGCTGCGAAAGCTTTTTCC‑AGAACAGGTT‑AGATGATTTTTTTGTCGCTTAATGCTTGTAAAACAGGCATGACCCAAAAAAAAATAAAAAAAA‑CCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGttt                                                                                             <  1:609183/150‑1 (MQ=255)
                                                                 tgctgcGCAGGCTTTTTCC‑AAAACAGTTT‑AGATGATTTTTTTTTCGTTTAATGCTTGAAAAACATGAATGAGCCAAAAAATAATAAAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGcc                                                                                          <  1:811081/147‑1 (MQ=255)
                                                                    tgcGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAAtt                                                                                   >  2:427820/1‑151 (MQ=255)
                                                                     gcgcAAGCTTTTCCA‑AAAACGGGTT‑AGATTATTTTTTTTTCGTTTAATCCCTAAAAAACATGAATGACCCAAAAAAAAATAAAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGaa                                                                                      <  2:86411/147‑1 (MQ=255)
                                                                     gcgcAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtc                                                                                  >  2:618941/1‑151 (MQ=255)
                                                                      cgcAGGCTTTTTCA‑AGAACAGGTT‑AGATGATTTTTTTGTCGCTTAATGCCTGAAAAACATGCATGAGCCAAAAAAAAAAAAAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAAt                                                                                    <  1:1087943/148‑1 (MQ=255)
                                                                      cgaaggctttttCA‑AGAAAAGGTT‑AAATGTTTTTTTTGTCGTTAATTCCCTAAAAAACTTGCATGACCCAAAAAAAAAAAAAAAAAC‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCa                                                                                         <  2:239919/135‑1 (MQ=255)
                                                                       gcAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgt                                                                                >  1:201647/1‑151 (MQ=255)
                                                                       gcAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgt                                                                                >  2:258341/1‑151 (MQ=255)
                                                                        cAAGCTTTTCCA‑AGAACAGGTA‑AGATGTTTTTTTTGTCGTTAAATCCTTAAAAAACATGCATGAGCCAAAAAATAATAAAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgt                                                                                <  1:932722/150‑1 (MQ=255)
                                                                          aaccttttCCAAGAACAGTTA‑AGATAATTTTTTTGTCGCTTATTGCCTAAAAAACATGAATGCACAAAAAAAAAATAAAAAAAA‑CCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgtc                                                                               <  2:727838/146‑1 (MQ=255)
                                                                          aGCGTTTTCA‑AAAAAAGGTT‑AGATGATTTTTTTGTCGTTTAATCCCTATAAAACATGAATGACCCAAAAAATAATAAAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgtcg                                                                              <  1:132597/150‑1 (MQ=255)
                                                                           gCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGt                                                                            >  1:1363346/1‑151 (MQ=255)
                                                                            cGTTTACA‑AGAATAGGTT‑AGATGTTTTTTTTGTCGCTAATTGCCTGAAAAACATGCATGACCAAAAAAAAAAAAAAAAAAC‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGt                                                                            <  1:483463/150‑1 (MQ=255)
                                                                              ttttCA‑AGAACAGGTA‑AGATAATTTTTTTGTCGTTTAATGCCTGAAAAACATGCATGACCCAAAAAATAATAAAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCgg                                                                             <  1:803899/147‑1 (MQ=255)
                                                                              tttgCC‑AAAACAGTTT‑AAATGTTTTTTTTTTCTTTTAAGGCTTAAAAAACGTGCATG‑GCCAAAAAAAAAAAAAAAAAAGCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgtc                                                                               <  2:470066/145‑1 (MQ=255)
                                                                              tttcCC‑AAAAAAGGTT‑AGATGTTTTTTTTGTCGTTTATTGCCTGAAAAACATGCATGACCCAAAAAATAATAAAAAAAT‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTaa                                                                          <  2:430791/150‑1 (MQ=255)
                                                                                   c‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGc                                                                        >  2:317731/1‑147 (MQ=255)
                                                                                     agaagaGGTT‑ATATTATTTTTTTGTCTCTTAATGCCTAAAAAACTGGCAGGAGCCAAAAAATAATAAAAAAAA‑CCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAg                                                                      <  2:1057588/148‑1 (MQ=255)
                                                                                          aGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAt                                                              >  1:949784/1‑151 (MQ=255)
                                                                                          aGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAt                                                              >  2:1059779/1‑151 (MQ=255)
                                                                                          aGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAt                                                              >  1:287695/1‑151 (MQ=255)
                                                                                           ggTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAtt                                                             >  1:1127501/1‑151 (MQ=255)
                                                                                            gTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTg                                                            >  2:1458858/1‑151 (MQ=255)
                                                                                             tt‑AGATGATTTTTTTGTCGTTAAATGCCTGAAAAACATGCAGGACCCAAAAAATAATAAAAAAAA‑CCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAt                                                              <  1:1389599/148‑1 (MQ=255)
                                                                                                  atgatCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGACATTCGTCGGTAAGCAGATTTGCATTGAttt                                                        >  2:1448520/1‑150 (MQ=255)
                                                                                                   tgtttttttttTCTTTTAATGCCTTAAAAACTTGCATGACCCACAAAAAAATAAAAAAAC‑CCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTg                                                            <  2:260963/140‑1 (MQ=255)
                                                                                                    gatCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACg                                                     >  2:74077/1‑151 (MQ=255)
                                                                                                       cTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtc                                                   >  2:276678/1‑150 (MQ=255)
                                                                                                       cTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtc                                                   >  2:618002/1‑150 (MQ=255)
                                                                                                         ttttttcttttAATGCATTAAAAACATTCGTGAACAAAAAAATAAAAAAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAtt                                                         <  1:184223/133‑1 (MQ=255)
                                                                                                           ttGTCCTTTAATCCCTGAAAAACATGAATGAGCCAAAAAAAAAAAAAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcatca                                               <  1:410966/150‑1 (MQ=255)
                                                                                                            tGTCGCTAAATGCCTATAAAACATGCATGGGCCAAAAAAAAATAAAAAAAA‑CCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTcatcat                                              <  1:323144/150‑1 (MQ=255)
                                                                                                               cttttAATGCCTAAAAAACATGCATGACCCAAAAAAAAATAAAAAAAA‑CCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCAtt                                             <  2:489869/145‑1 (MQ=255)
                                                                                                                 cTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGa                                         >  2:320405/1‑150 (MQ=255)
                                                                                                                  ttAATGCCTGAAAAACATGCATGAGCCAAAAAAAAATAAAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGaa                                        <  1:944325/150‑1 (MQ=255)
                                                                                                                  ttAATCCCTAAAAAACAGGCAGGAGCCAAAAAATAATAAAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgtg                                          <  1:441545/148‑1 (MQ=255)
                                                                                                                    attccttgaaaaaCATCCATGAGCCAAAAAAAAATAAAAAAAA‑CCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGa                                         <  2:1096825/138‑1 (MQ=255)
                                                                                                                    aatccctaaaaaaCATGCATGAGCCAAAAAATAATAAAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAt                                       <  2:489069/140‑1 (MQ=255)
                                                                                                                    aaTGCCTGAAAAACATGCAGGAGCAAAAAAAAAATAAAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAt                                       <  1:1458902/149‑1 (MQ=255)
                                                                                                                      tGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAat                                   >  2:1140790/1‑151 (MQ=255)
                                                                                                                           gTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATg                                >  1:919421/1‑149 (MQ=255)
                                                                                                                             aaaaGATGCATATGCCAAAAAAAAATATAAAAAC‑GCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGTAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCaa                             <  2:597058/150‑1 (MQ=255)
                                                                                                                             aaaaCATGCATGGCCCAAAAAATAAAAAAAAAAA‑CCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCa                              <  1:722382/149‑1 (MQ=255)
                                                                                                                             aaaaCATGCATGAGCCAAAAAATAATAAAAAAAA‑CCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAatat                                 <  2:163964/146‑1 (MQ=255)
                                                                                                                             aaaaCATGCATGACCAAAAAAAAAATAAAAAAAT‑CCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCa                              <  2:636462/149‑1 (MQ=255)
                                                                                                                              aaccttgcatgAGCCAAAAAATAATAAAAAAAA‑CCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGc                               <  1:1097856/142‑1 (MQ=255)
                                                                                                                              aaccatgcagcggCCAAAAAAAAATATAAAAAT‑ACCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCaa                             <  1:634067/137‑1 (MQ=255)
                                                                                                                                    gcaggAGCAAAAAAAAAATAAAAAAAA‑CCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg                     <  1:811094/151‑1 (MQ=255)
                                                                                                                                     catgACCCAAAAAATAATAAAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg                     <  2:1348250/150‑1 (MQ=255)
                                                                                                                                      atgACCCAAAAAAAAATAAAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg                     <  1:974670/149‑1 (MQ=255)
                                                                                                                                       ggacccAAAAAATAATAAAAAAAA‑CCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagt                  <  1:1330260/147‑1 (MQ=255)
                                                                                                                                         aGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgtgtg                 >  1:851021/1‑150 (MQ=255)
                                                                                                                                         aGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagtg                 >  1:314126/1‑150 (MQ=255)
                                                                                                                                          cccAAAAAATAAAAAAAAAAC‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgag                   <  1:177688/146‑1 (MQ=255)
                                                                                                                                             aaaaaaaaataaaaaaat‑CCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAata             <  2:1053811/148‑1 (MQ=255)
                                                                                                                                             aaaaaaaaATAAAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAata             <  2:165217/148‑1 (MQ=255)
                                                                                                                                             aaaaaaTAATAAAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagtg                 <  2:322439/144‑1 (MQ=255)
                                                                                                                                               aaaaTAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtc          >  1:1093023/1‑151 (MQ=255)
                                                                                                                                               aaaaCAAGATAAAGAA‑TCCCGCCATTAAGTTGTCTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagtg                 <  2:220905/144‑1 (MQ=255)
                                                                                                                                                aaataaaataAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtct         <  1:501755/151‑1 (MQ=255)
                                                                                                                                                aaaTAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtct         >  1:257155/1‑151 (MQ=255)
                                                                                                                                                 aataatAAAAAAAC‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtctc        <  2:1363306/151‑1 (MQ=255)
                                                                                                                                                 aataatAAAAAAAA‑CCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtctc        <  2:1310177/151‑1 (MQ=255)
                                                                                                                                                  ataataTAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTctct       >  1:269359/1‑151 (MQ=255)
                                                                                                                                                  aaaataAAAAAAT‑CCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTctct       <  2:1343981/149‑1 (MQ=255)
                                                                                                                                                    aataTAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTgg     >  2:960215/1‑151 (MQ=255)
                                                                                                                                                      tataAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTgg     >  2:1671/1‑149 (MQ=255)
                                                                                                                                                      tataAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTgg     >  1:1263105/1‑149 (MQ=255)
                                                                                                                                                       ataAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAgg  >  2:748181/1‑151 (MQ=255)
                                                                                                                                                        |                                                                                                                                                      
TTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAA‑TCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAGG  >  NC_000913/29346‑29645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑