Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,434,882	0	T	G	42.9%	157.4 / 25.7	91	Y252S (TAC→TCC)	truA	tRNA pseudouridine(38‑40) synthase
Reads supporting (aligned to +/- strand):  ref base T (9/43);  new base G (39/0);  total (48/43)
Fisher's exact test for biased strand distribution p-value = 2.10e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAC  >  NC_000913/2434738‑2435027                                                                                                                                                 |                                                                                                                                                   ccATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGGCAGGGGAATcc                                                                                                                                               >  1:489026/1‑150 (MQ=255) ccATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGAACATACCGGGCAGGGGAAttc                                                                                                                                               >  1:17146/1‑148 (MQ=255) ccATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGCTCATAACGGTCAGGGGaa                                                                                                                                                  >  2:1029815/1‑147 (MQ=255) ccATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATcc                                                                                                                                               <  1:138402/150‑1 (MQ=255) ccATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGACCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGGCCGGGGAATcc                                                                                                                                               >  1:456975/1‑150 (MQ=255)  cATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCAc                                                                                                                                             <  1:786975/151‑1 (MQ=255)   atatTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAAATCATACCCGGCCGGGGAATCCAcc                                                                                                                                            >  2:864628/1‑151 (MQ=255)    tatTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGGCAGGGGAATCCAcc                                                                                                                                            >  1:254646/1‑150 (MQ=255)    tatTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGGCAGGGGAATCCAcc                                                                                                                                            >  1:276235/1‑150 (MQ=255)     atTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACcgc                                                                                                                                          <  1:1343643/151‑1 (MQ=255)         gCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGAcc                                                                                                                                      <  2:814517/151‑1 (MQ=255)          ccTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCCGGCAGGGGAATCCACcccg                                                                                                                                         >  1:1098071/1‑147 (MQ=255)           cTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGAACATAACCGTCAGGGGAATCCACCCCGAcc                                                                                                                                      >  2:1391652/1‑149 (MQ=255)             aTGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATAACGGTCAGGGGGAACCACCCCGGcc                                                                                                                                      >  2:1458717/1‑147 (MQ=255)             aTGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCCGGCCGGGGAATCCACCGCGAcc                                                                                                                                      >  1:1286156/1‑147 (MQ=255)                ccTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCAAAACCGGCAGGGGAATCCACCGCGACCAGATa                                                                                                                                 >  1:802062/1‑149 (MQ=255)                  ttGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACa                                                                                                                               >  1:374521/1‑149 (MQ=255)                   tGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑g                                                                                                                             <  2:582257/149‑1 (MQ=255)                    gTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑Gccc                                                                                                                          <  2:37236/151‑1 (MQ=255)                       cTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCCGGCCGGGGAATCCACCCCGACCCGATACA‑Gcccct                                                                                                                        >  2:380186/1‑148 (MQ=255)                         cGTCATCTCTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTc                                                                                                                       <  1:1074809/149‑1 (MQ=255)                            cATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCt                                                                                                                   <  2:1089795/150‑1 (MQ=255)                               cTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGTCAGGGGAATCCACCCCGGCCAGATACA‑ACCCTTCCGCtttt                                                                                                                >  1:467865/1‑150 (MQ=255)                                 ttCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATAATACCCGGCAGGGGAATCCACCCCGACCACATACA‑GCCCCTCCCCcttt                                                                                                                >  2:986997/1‑148 (MQ=255)                                 ttCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCtttt                                                                                                                <  1:141297/148‑1 (MQ=255)                                 ttCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTg                                                                                                               <  2:1384610/149‑1 (MQ=255)                                         gTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTTCAGATGATACCGCCGGGGGGAATTCACCCCGAACAGAAAAATGCCCTTCTCCtttgtc                                                                                                              >  2:1255681/1‑140 (MQ=255)                                           aaCTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGGCCGGGGAAACCCCCCCGGCCCGATACA‑GCCCTTCCGCTTTTGCCGTTGCTgcc                                                                                                    >  2:401649/1‑150 (MQ=255)                                           aaCTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTgcc                                                                                                    <  1:1152390/150‑1 (MQ=255)                                             cTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATAACCGGCCGGGGAATCCACCCCGACCAGATACA‑ACCCTTCCGCTTTTTCCGTTGCTgccgcc                                                                                                 >  2:1021058/1‑151 (MQ=255)                                               aCGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCCGTCAGGGGAAACCCCCGCGACCAGATACA‑GCCCTTCCCCTTTTTCCGTTGCTgccccc                                                                                                 >  1:159714/1‑149 (MQ=255)                                                cGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGc                                                                                              <  2:1000759/151‑1 (MQ=255)                                                 gTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATTATAACCGTCAGGGGAATCCACCCCGGCCAGATACA‑GCCCTTTCGCCTTTTCCGTTGCTGCCGCCa                                                                                                >  2:977134/1‑148 (MQ=255)                                                    ggCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGtt                                                                                           <  2:115511/150‑1 (MQ=255)                                                     gCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACA‑GCCCCTCCGCTTTTGCCGCTGCTGCCGCCcgcggt                                                                                           >  1:1229929/1‑147 (MQ=255)                                                      cTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATTATACCGGGCAGGGGAAACCACCGCGGCCAGATACA‑ACCCCTCCCCTTTTTCCGGTGCTGGCGCCAGCggt                                                                                           >  2:799798/1‑146 (MQ=255)                                                       tACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGGCAGGGTAATCCACCCCGACCAGATACA‑GCCCCTCCGCTTTTGTCGTTGCTGCCGCCCGCGTTCt                                                                                         >  1:1202576/1‑149 (MQ=255)                                                       tACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATAACGGGCAGGGGAAACCACCCCGACCAGAAAAA‑ACCCTTCCGCCTTTTCCCTTGCTGCCGCCcgcggt                                                                                           >  1:599014/1‑145 (MQ=255)                                                          aGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGAACATACCGGGCAGGGGAAATCACCGCGACCAGATAAA‑ACCCTTCCCCCTTTTTCGTTGCTGCCCCCAGCGGTCTGTcc                                                                                     >  1:1249284/1‑150 (MQ=255)                                                            ccTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACA‑GCCCCTCCGCTTTTTCCGTTGCTGCCGCCAGCGGTCTGTCCttt                                                                                  >  1:466637/1‑151 (MQ=255)                                                                aaaTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAAATCATAACGGTCAGGGGAAACCACCGCGACCAGATACA‑GACCTTCCGCTTTTGCCGTTGCTGCCCCCAGCGGTCTTTCCTTTgcc                                                                               >  1:1107546/1‑150 (MQ=255)                                                                   tGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCAGTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTGCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTgcctcc                                                                            <  2:223871/150‑1 (MQ=255)                                                                    gTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAg                                                                          <  1:275354/151‑1 (MQ=255)                                                                        cGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATTATATCGGTCAGGGGAATCCACCGCGACCAGATAAA‑GCCCCTCCGCTTTTGTCGTTGCTGCCGCCAGCGGTCTGTCCTTTTccgcccgc                                                                         >  2:1107856/1‑148 (MQ=255)                                                                           gCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAActct                                                                   <  2:1081744/151‑1 (MQ=255)                                                                                 ttCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGGCAGGGGAATCCACCCCGACCCGATACA‑GCCCTTCCGCTTTTTCCGGTGCTGCCCCCCGCGTTCTGTCCTTTGCCGCCAGCCACCCTGCTAt                                                              >  2:376017/1‑150 (MQ=255)                                                                                   ctcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCCTACCCGTCAGGGGAAACCACCGCGACCAGATACA‑GCCCCTCCGCTTTTGCCGTTGCTGCCGCCCGCGGTCTGTCCTTTTCCGCCCGCAAATCTGCTATcc                                                            >  1:1118835/1‑150 (MQ=255)                                                                                   ctcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCCCGACCAGATACA‑GCCCTTCCCCTTTTTCCGTTGCTGCCGCCCGCGGTTTTTCCTTTTCCCCCCGCAAATCTGCTATcc                                                            >  1:236053/1‑150 (MQ=255)                                                                                     cGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATcc                                                            <  1:1021083/148‑1 (MQ=255)                                                                                     cGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAg                                                          <  2:254643/150‑1 (MQ=255)                                                                                      gTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGct                                                        <  2:1123238/151‑1 (MQ=255)                                                                                      gTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATAAA‑ACCCCTCCGCTTTTTCCGTTGCTGCCCCCAGCGGTCTGTCCTTTTTCGCCAGCAACTCTGCTAttc                                                            >  1:949746/1‑145 (MQ=255)                                                                                         aGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGAATATACCGGTCAGGGGAATTCACCGCGAACAGAAACA‑ACCCTTCCGCCTTTGTCGGTTCTGCCCCCCGCCTTTTGTCCTTTGCCCCCAGCAAATCTGCCTTCCACctc                                                       >  1:977960/1‑149 (MQ=255)                                                                                         aGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCtctc                                                     <  2:1406142/151‑1 (MQ=255)                                                                                          gTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTcc                                                    <  1:254542/151‑1 (MQ=255)                                                                                            ccGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGAACATACCCGGCCGGGGAATCCACCCCCGCCCGATACA‑GCCCCTCCCCTTTTTCCGTTGCCGCCCCCCCCGGTTTTTCCTTTTTCGCCCGCAACTCTTCTATCCAGctcctc                                                    >  2:32867/1‑146 (MQ=255)                                                                                                cAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTgg                                              <  1:21240/151‑1 (MQ=255)                                                                                                 aGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGt                                             <  2:1249252/151‑1 (MQ=255)                                                                                                  gAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGTCAGGGGAATACACCCCGACCAGATACA‑ACCCTTCCGCTTTTTCCGTTTCTGCCCCCCGCGTTCTGTCCTTTTCCGCCCGCCACCCTGCTATTCAGCCCTCCGGCTgg                                              >  1:305672/1‑149 (MQ=255)                                                                                                  gAAATAGCGGGCCCATTGGCGGTTTTTGAAGATAATAACGGGCAGGGGAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTTCCGTTGCTGCCGCCAGCGGTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTgg                                              >  1:1036250/1‑149 (MQ=255)                                                                                                  gAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGtt                                            <  1:278210/151‑1 (MQ=255)                                                                                                     aTAGCGGGCCCATTGGCGGTTTTTGGAGATCAAACCCGTCAGGGGAATCCACCCCGACCAGATACA‑ACCCTTCCGCTTTTTCCGGTGCTGCCGCCCGCGGTCTTTCCTTTTCCGCCCGCCACCCTGCTATCCACCTCTCCCGCTGGgtgt                                          >  2:261793/1‑150 (MQ=255)                                                                                                      tAGCGGGCCCATTGGCGGTTTTTGGAAATCATACCGGGCAGGGGAATCCACCCCGACCCGATACA‑ACCCTTCCGCCTTTTCCGGTGCTTCCCCCCGCGGTCTTTTCTTTTGCCCCAGCCACCCTGCTTTTCACCTCTCCGGCTGGgtgt                                          >  2:1151434/1‑149 (MQ=255)                                                                                                      tAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCCGTCAGGGGAATTCACCGCGACCAGATACA‑GCCCCTCCGCCTTTTCCGGTGCTGCCGCCCGCGGTCTTTCCTTTGCCGCCCGCAACTCTGCTATCCAGCCCTCCCGCTGGGTGTgg                                        >  2:325749/1‑151 (MQ=255)                                                                                                       aGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTgg                                        <  1:864650/150‑1 (MQ=255)                                                                                                        gCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTgg                                        <  1:54006/149‑1 (MQ=255)                                                                                                        gCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGc                                      <  1:343627/151‑1 (MQ=255)                                                                                                                aTTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACtt                              <  1:32867/151‑1 (MQ=255)                                                                                                                  tGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTcc                            >  1:949088/1‑151 (MQ=255)                                                                                                                   ggCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTcc                            <  1:1458761/150‑1 (MQ=255)                                                                                                                   ggCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTcc                            <  2:489019/150‑1 (MQ=255)                                                                                                                   ggCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTcc                            >  2:897227/1‑150 (MQ=255)                                                                                                                       gTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATc                         >  1:818189/1‑149 (MQ=255)                                                                                                                          ttGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGc                     <  2:1172079/150‑1 (MQ=255)                                                                                                                          ttGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCt                    >  2:272880/1‑151 (MQ=255)                                                                                                                             gAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGcc                 <  2:1198577/151‑1 (MQ=255)                                                                                                                             gAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGcc                 <  1:1198927/151‑1 (MQ=255)                                                                                                                              aaGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCg                >  1:999520/1‑151 (MQ=255)                                                                                                                                gATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGAc              <  1:165586/151‑1 (MQ=255)                                                                                                                                 aTCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACa             <  2:514788/151‑1 (MQ=255)                                                                                                                                   cATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACaa            <  2:742929/150‑1 (MQ=255)                                                                                                                                   cATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAat           <  2:894384/151‑1 (MQ=255)                                                                                                                                     tACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAatat         >  2:714869/1‑151 (MQ=255)                                                                                                                                      aCCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATAtt        <  2:467858/151‑1 (MQ=255)                                                                                                                                       ccGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATAtt        <  2:456968/150‑1 (MQ=255)                                                                                                                                       ccGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTc       >  2:449367/1‑151 (MQ=255)                                                                                                                                        cGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTcc      <  2:670777/151‑1 (MQ=255)                                                                                                                                        cGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTcc      >  2:1302434/1‑151 (MQ=255)                                                                                                                                          gTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTg    <  2:276232/151‑1 (MQ=255)                                                                                                                                            cAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAc  <  2:576565/151‑1 (MQ=255)                                                                                                                                             aGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAc  <  1:1301592/150‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                   CCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAC  >  NC_000913/2434738‑2435027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑