Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,624,517	0	G	T	45.1%	86.6 / 42.7	72	E492* (GAA→TAA)	ppk	polyphosphate kinase, component of RNA degradosome
Reads supporting (aligned to +/- strand):  ref base G (8/31);  new base T (32/0);  total (41/31)
Fisher's exact test for biased strand distribution p-value = 5.14e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGTA‑TTTAAC‑TTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTA‑TCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCG  >  NC_000913/2624372‑2624667                                                                                                                                                     |                                                                                                                                                        tAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGTA‑TTTTAC‑TTTATTTaaaa                                                                                                                                                     >  2:1245025/1‑150 (MQ=255) tAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGGA‑TTTTAC‑TTTTTTTaaaa                                                                                                                                                     >  1:1012598/1‑150 (MQ=255)   aaGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGTA‑TTTTAC‑TTTTTTTAAAAccc                                                                                                                                                  >  2:546888/1‑151 (MQ=255)      aaaaCGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGTA‑TTTAAC‑TTTATTGAAAACCCATa                                                                                                                                               <  2:262275/151‑1 (MQ=255)         aCGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGGA‑TTTTAA‑TTTTTTTTAAAACCATa                                                                                                                                               >  1:938091/1‑148 (MQ=255)         aCGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGGA‑TTTTAA‑TTTTTTTAAAACCCATAc                                                                                                                                              >  2:666366/1‑149 (MQ=255)            gTGAAGTGGTGCGTTATGCTCACTTCGGGACAGGGAACTTTAACGAAAAACCCGCGCGTCTTTATACTGTATATTCATTGCTTACCGCCGATGCGCGCGTCACCAACGAAGTACGGC‑GGGTA‑TTTAAC‑TTTATTGAAAACCCATACCGTcc                                                                                                                                         <  2:772891/151‑1 (MQ=255)            gTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGGA‑TTTAAA‑CTTTTTTTAAACCCCCACCGTcc                                                                                                                                         >  2:272176/1‑151 (MQ=255)            gTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGCA‑TTTGAA‑TTTTTTTTAAACCCCCACCgcgc                                                                                                                                         >  1:901694/1‑148 (MQ=255)              gAAGTGGTGCTTTATCCACCCATCGGGACCGGGATCTTTAACGAAAAAACCGCGCGTCTTTATCCTGTCTATTCGTTGCTTACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGTA‑TTTAAC‑TTTATTGAAAACCCATACCGTcc                                                                                                                                         <  1:145358/149‑1 (MQ=255)               aaGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGTA‑TTTAAC‑TTTATTGAAAACCCATACCGTCCGGt                                                                                                                                      <  2:511904/151‑1 (MQ=255)               aaGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGGC‑TTTTAC‑TTTTTTTAAAAACCACACCGTcccg                                                                                                                                       >  1:793088/1‑148 (MQ=255)                   ggtgCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGTA‑TTTAAC‑TTTATTGAAAACCCATACCGTCCGGTGACa                                                                                                                                  <  1:640445/151‑1 (MQ=255)                       cGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGTATTTTAAT‑TTTTTTTAAAACCC‑TACCGTCCGGGGACAtttt                                                                                                                              >  1:1032265/1‑150 (MQ=255)                       cGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGTA‑TTTAAC‑TTTATTGAAAACCCATACCGTCCGGTGACAttt                                                                                                                               <  1:64597/150‑1 (MQ=255)                        gTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGGA‑TTTTAC‑TTTATTTTAAAACCCTACCCTCCGGGGGCAtttt                                                                                                                              >  1:156241/1‑149 (MQ=255)                           aTGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGTA‑TTTAAC‑TTTATTGAAAACCCATACCGTCCGGTGACATTTGatt                                                                                                                           <  1:287733/150‑1 (MQ=255)                            tGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGAA‑TTTAAA‑TTTTTTTATAAACCATACCGTACggcgcgacttttat                                                                                                                           >  2:525299/1‑137 (MQ=255)                              cacacaTCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGGA‑TTTTAC‑TTTTTTTTAAAACCCTACCCTCCCGGGGCATTTTATTAttt                                                                                                                       >  1:503809/1‑151 (MQ=255)                              cacacaTCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGGA‑TTTTAC‑TTTATTTAAAACCCCCACCCTCCCGGGACATTTGAtttttt                                                                                                                       >  2:457550/1‑151 (MQ=255)                               acccaccGGGACCGGGAACTTTACCGAAAACACCGCGCGTCTTTACACTGACTATCCGTTGCTGACCGCCGATGCGCGCATCTCCAACGAAGTACGGC‑GGGTA‑TTTAAC‑TTTATTGAAAACCCATGCCGTCCGGTGACATTTGat                                                                                                                            <  2:263044/139‑1 (MQ=255)                                cacaTCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGGT‑TTTAAC‑TTT‑TTTAAAAACCATAACGTCCGGGGGCATTTTGTTAttt                                                                                                                       >  1:1240171/1‑149 (MQ=255)                                cacaTCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGGATTTTAAT‑TTTTTTGAAACCCCCAACCG‑CCGGGGGGATTTTAtttttt                                                                                                                       >  1:1142247/1‑149 (MQ=255)                                   aTCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGGA‑TTTACT‑TTTTTTTAAAACCCTACCCGCCCGGGGACtttttttattt                                                                                                                        >  2:1312372/1‑139 (MQ=255)                                   aTCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGTA‑TTTAAC‑TTTTTTTAAAAACCCAACCGTCCGGGGGGATTTGATTTTTTTAt                                                                                                                    >  2:1459572/1‑149 (MQ=255)                                     cGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGGA‑TTTTAA‑TTTTTTTAAAACCCATAACCTCCCGGGGCATTTTAttttttt                                                                                                                      >  1:1355811/1‑144 (MQ=255)                                        gACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGGA‑TTTAAA‑TTTTTTTAAAAACCAAACCGTCCGGGGGCATTTTATTTTTTTATGGttt                                                                                                               >  1:287688/1‑147 (MQ=255)                                         aCCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGTA‑TTTTAC‑TTTTTTGAAAAACCCTACCGTCCGGGGACATTTGtttattttat                                                                                                                    >  1:993404/1‑143 (MQ=255)                                          ccGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGTA‑TTTTAC‑TTTTTTTTAAAACCCTACCCTCCGGGGACCTTTTATTATTTTATGGTA‑Tcgcc                                                                                                          >  2:89383/1‑151 (MQ=255)                                          ccGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGTA‑TTTAAC‑TTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTA‑Tcgcc                                                                                                          <  2:1359919/151‑1 (MQ=255)                                           cGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGTA‑TTTAAC‑TTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTA‑Tcgccg                                                                                                         <  1:980822/151‑1 (MQ=255)                                            gggAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGTA‑TTTAAC‑TTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTA‑Tcgccgc                                                                                                        <  1:1242770/151‑1 (MQ=255)                                             ggAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGTA‑TTTAAC‑TTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTA‑TCGCCGCa                                                                                                       <  1:529484/151‑1 (MQ=255)                                              gAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGGA‑TTTTAC‑TTTTTTTAAAAACCATACCGTCCGGGGACATTTTATTATTTTATGGTA‑Tcgccgc                                                                                                        >  1:8345/1‑149 (MQ=255)                                                   ttAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGTA‑TTTTACTTTTTTTTAAAACCCA‑ACCCTCCGGGGGCATTTTATTTTTTAATTGGA‑TTGCCGCcaaa                                                                                                    >  2:1185065/1‑148 (MQ=255)                                                      aCGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGGT‑TTTTAA‑TTTTTTGAAAAACCCTACCGACCGGCGGCGTTTTATTTTTTTATGGTA‑TCGGCGCCAAAATccc                                                                                               >  1:258394/1‑150 (MQ=255)                                                        gAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGGA‑TTTTAA‑TTTTTTTAAAAACCCTACCCTCCGGGGGCATTTTATTTTTTTATTGGA‑TAGCCCCAAAAATCccgcc                                                                                            >  2:1190221/1‑151 (MQ=255)                                                           aaaaCCGCGCGTCTTTATACTGACTATTCGTTGCTGCCCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGTA‑TTTAAC‑TTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTA‑TCGCCGCAAAACTCCCgccgcc                                                                                         <  1:205617/151‑1 (MQ=255)                                                            aaaCCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGTA‑TTTAAC‑TTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTA‑TCGCCGCAAAACTCCCGCCGCCt                                                                                        <  2:598022/151‑1 (MQ=255)                                                            aaaCCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGGA‑TTTTAC‑TTTTTTTAAAAACCATACCCTCCGGGGACATTTTATTATTTAATGGTA‑TCGCCGCAAAACTCCCgccgcc                                                                                         >  1:440283/1‑150 (MQ=255)                                                            aaaCCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGCGA‑TTTTAC‑TTTATTTTAAAACCATACCGTCCGGGGGCAATTGATTATTTTATGGTG‑TTGACGCaaaaaaccccccccc                                                                                         >  1:44296/1‑139 (MQ=255)                                                             aaCCGCGCTTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGTA‑TTTAAC‑TTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTA‑TCGCCGCAAAACTCCCGCCGCCTa                                                                                       <  2:889998/151‑1 (MQ=255)                                                                   gcgTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGTA‑TTTAAC‑TTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTA‑TCGCCGCAAAACTCCCGCCGCCTATTGTa                                                                                  <  2:170836/150‑1 (MQ=255)                                                                         ttATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGTA‑TTTAAC‑TTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTA‑TCGCCGCAAAACTCCCGCCGCCTATTGTATGaa                                                                              <  1:89552/148‑1 (MQ=255)                                                                          tataCTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGGA‑TTTTAC‑TTTTTTTTAAAACCCTACCGTCCGGGGGCATTTTATTTTTTTATTGTA‑TTGCCCCCAAACACCCCCCGCCCATTTTATGAAAagg                                                                          >  1:1089847/1‑151 (MQ=255)                                                                             aCTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGTA‑TTTAAC‑TTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTA‑TCGCCGCAAAACTCCCGCCGCCTATTGTATGAAAtggtgg                                                                       <  1:8779/151‑1 (MQ=255)                                                                              cTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGTA‑TTTAAC‑TTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTA‑TCGCCGCAAAACTCCCGCCGCCTATTGTATGAAAtgg                                                                          <  1:744980/147‑1 (MQ=255)                                                                                   tATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGTA‑TTTAAC‑TTTTTTTAAAACCCCAACCCCCCCGGGGCATTTTTTTTTTTTATTGTT‑TTGCCCCCAAACTCCCCCCCCCCCTTTTTTGAAAttgggg                                                                       >  2:156500/1‑140 (MQ=255)                                                                                         ttGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGTA‑TTTAAC‑TTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTA‑TCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATc                                                            <  2:760552/150‑1 (MQ=255)                                                                                         ttGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGGA‑TTTAAC‑TTTTTTTAAAAACCATACCGTCCGGGGGCATTTTATTTTTTTATGGGA‑TCGCCCCCAAACCCCCCCCCCCCATTGTTTTAAATGGGGGACCCCgggaac                                                            >  1:1098169/1‑148 (MQ=255)                                                                                          tGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGGA‑TTTTAC‑TTTTTTTAAAAACCCAACCGTCCGGGGGCATTTTATTTTTTTATGGTA‑TTGCCGCAAAAATCCCCCCGCCCATTTTATGAAAAGGGGGGACGCGAGGTCg                                                           >  1:1356872/1‑150 (MQ=255)                                                                                              gACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGGA‑TTTTAC‑TTTATTTAAAAACCCTACCGGCCGGGGGCATTTTATTTTTTTATGGGA‑TTGCCGCAAAACACCCCCCGCCCCTTTTATGAAAAGGGGGGCCGCGAGAACGCCaa                                                       >  1:1250307/1‑150 (MQ=255)                                                                                                  gccgATGCGCGCATCACCAACGAAGTACGGC‑GGGGC‑TTTTAA‑TTTATTTAAAACCCACACCGGCCCGGGGCAATTTGTTTTTTTATTGTT‑TCGCCCCAAAAATCCCCCCCCCCCTTTTATTAAAAGGGGGGCCGCGGGATCGcc                                                         >  1:106772/1‑144 (MQ=255)                                                                                                  gccgATGCGCGCATCACCAACGAAGTACGGC‑GGGGATTTTAAT‑TTTTTTAAAAACCCTACCCAGCCGGGGA‑ATTTTATTATTTTATGGGATTTCCCCCAAAAATCCC‑CCCCCCATTTTATGTAATTGTGGGACCCGAGAGTGCCAAccc                                                    >  1:56133/1‑147 (MQ=255)                                                                                                         cgcgcATCACCAACGAAGTACGGC‑GGGTA‑TTTAAC‑TTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTA‑TCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAgg                                           <  1:504922/151‑1 (MQ=255)                                                                                                         cgcgcATCACCAACGAAGTACGGC‑GGGTA‑TTTAAC‑TTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTA‑TCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAgg                                           <  1:1368258/151‑1 (MQ=255)                                                                                                                cACCAACGAAGTACGGC‑GGGTA‑TTTAAC‑TTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTA‑TCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGcc                                     <  1:272179/150‑1 (MQ=255)                                                                                                                  ccAACGAAGTACGGC‑GGGGA‑TTTTAC‑TTTTTTTAAAAACCATACCCGCCGGGGGCATTTTATTATTTTATTGTA‑TCGCCGCAAAAATCCCCCCGCCTATTTTATTAAAAGGGGGACCGCGAGATCGCCAACGCGCAGCAAGGGGTGccc                                    >  1:342122/1‑149 (MQ=255)                                                                                                                        aaGTACGGC‑GGGTA‑TTTAAC‑TTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTA‑TCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTa                             <  1:587716/150‑1 (MQ=255)                                                                                                                          gTACGGC‑GGGTA‑TTTAAA‑TTTATTTAAAACCCATACCGACCGGGGACCTTTGATTATTTAATGGGA‑TCGCCGCAAAAATCCCGCCGCCCATTGTATGAAATGGTGGACCGCGCGATCGCCAACGCGCAGCAAGGGGTGCCCAATGGTAt                            >  2:1301528/1‑149 (MQ=255)                                                                                                                            aCGGC‑GGGTA‑TTTAAC‑TTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTA‑TCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCAcc                        <  2:938068/151‑1 (MQ=255)                                                                                                                            aCGGC‑GGGTA‑TTTAAC‑TTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTA‑TCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCAcc                        <  2:197291/151‑1 (MQ=255)                                                                                                                            aCGGC‑GGGTA‑TTTAAC‑TTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTA‑TCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCAc                         <  2:786883/150‑1 (MQ=255)                                                                                                                                c‑ggGTA‑TTTAAC‑TTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTA‑TCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGa                    <  1:1067210/151‑1 (MQ=255)                                                                                                                                      a‑TTTAAC‑TTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTA‑TCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCt                >  2:630022/1‑150 (MQ=255)                                                                                                                                      a‑TTTAAC‑TTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTA‑TCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTa               <  1:1245057/151‑1 (MQ=255)                                                                                                                                         ttAAC‑TTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTA‑TCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTaa              >  2:795207/1‑150 (MQ=255)                                                                                                                                           aaC‑TTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTA‑TCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAAt            >  2:895456/1‑150 (MQ=255)                                                                                                                                           aaC‑TTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTA‑TCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATa           <  1:1090200/151‑1 (MQ=255)                                                                                                                                           aaC‑TTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTA‑TCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATa           <  1:787412/151‑1 (MQ=255)                                                                                                                                                  aTTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTA‑TCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTg     >  1:88416/1‑151 (MQ=255)                                                                                                                                                    tGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTA‑TCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTc   >  2:990534/1‑151 (MQ=255)                                                                                                                                                     gAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTA‑TCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCg  <  1:496403/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                        TAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGC‑GGGTA‑TTTAAC‑TTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTA‑TCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCG  >  NC_000913/2624372‑2624667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑