Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,186,551	T→G	V401G (GTT→GGT)	rpoC →	RNA polymerase, beta prime subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,186,551	0	T	G	100.0%	398.2 / NA	95	V401G (GTT→GGT)	rpoC	RNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (48/47);  total (48/47)

TCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGC  >  NC_000913/4186406‑4186697                                                                                                                                                  |                                                                                                                                                   tCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAg                                                                                                                                                <  1:909081/150‑1 (MQ=255)    gTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAgcgc                                                                                                                                             <  1:748498/150‑1 (MQ=255)    gTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAgcgc                                                                                                                                             <  1:1398071/150‑1 (MQ=255)    gTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGcgcg                                                                                                                                            <  2:147216/151‑1 (MQ=255)    gTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGcgcg                                                                                                                                            >  2:1460864/1‑151 (MQ=255)    gTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGcgcg                                                                                                                                            <  1:1102082/151‑1 (MQ=255)      aaTCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCgaa                                                                                                                                          <  1:1416150/151‑1 (MQ=255)          aCCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCgaaga                                                                                                                                        >  1:1113179/1‑149 (MQ=255)           ccGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGc                                                                                                                                     >  1:1014796/1‑151 (MQ=255)           ccGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGc                                                                                                                                     <  1:357797/151‑1 (MQ=255)             gTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTg                                                                                                                                   >  1:836789/1‑151 (MQ=255)              tAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGt                                                                                                                                  >  2:979639/1‑151 (MQ=255)                   ccATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGttt                                                                                                                             <  2:337431/151‑1 (MQ=255)                   ccATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGttt                                                                                                                             <  2:1221026/151‑1 (MQ=255)                      tACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTggg                                                                                                                          >  1:1006192/1‑151 (MQ=255)                       aCCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTggg                                                                                                                          >  2:317157/1‑150 (MQ=255)                         cTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGat                                                                                                                        >  2:1213143/1‑150 (MQ=255)                           gCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGatat                                                                                                                      <  1:49815/150‑1 (MQ=255)                           gCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGata                                                                                                                       <  1:390002/149‑1 (MQ=255)                             gTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCt                                                                                                                   >  1:448584/1‑151 (MQ=255)                               cTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTgg                                                                                                                 >  2:247112/1‑151 (MQ=255)                                 gCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTgg                                                                                                                 <  2:748377/149‑1 (MQ=255)                                   aTCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACg                                                                                                              >  2:1315720/1‑150 (MQ=255)                                     cAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGaa                                                                                                            <  2:1160667/150‑1 (MQ=255)                                      aGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGt                                                                                                          <  1:178231/151‑1 (MQ=255)                                            gTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCcg                                                                                                    >  1:673241/1‑151 (MQ=255)                                               tGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCcgcg                                                                                                  >  2:1193/1‑150 (MQ=255)                                                gCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCcgc                                                                                                   <  2:148821/148‑1 (MQ=255)                                                gCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCcgc                                                                                                   <  1:125882/148‑1 (MQ=255)                                                gCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGa                                                                                                 >  1:239245/1‑150 (MQ=255)                                                   gaagaaAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAacac                                                                                             >  2:703762/1‑151 (MQ=255)                                                     agaaAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACAccc                                                                                           <  2:1313327/151‑1 (MQ=255)                                                     agaaAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACAccc                                                                                           <  2:125768/151‑1 (MQ=255)                                                     agaaAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACAccc                                                                                           <  1:832677/151‑1 (MQ=255)                                                     agaaAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACAccc                                                                                           <  1:710520/151‑1 (MQ=255)                                                            ggCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTActg                                                                                    <  2:24177/151‑1 (MQ=255)                                                            ggCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTActg                                                                                    <  2:776088/151‑1 (MQ=255)                                                             gCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTActgc                                                                                   <  1:1103006/151‑1 (MQ=255)                                                              cACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTActgct                                                                                  <  2:1047157/151‑1 (MQ=255)                                                                 tGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGa                                                                                >  2:45220/1‑150 (MQ=255)                                                                 tGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGCGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTActgctg                                                                                 >  1:10853/1‑149 (MQ=255)                                                                   gAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAAcc                                                                             >  1:213170/1‑151 (MQ=255)                                                                        gTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCa                                                                        <  2:4481/151‑1 (MQ=255)                                                                         ttCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCAc                                                                       >  2:583681/1‑151 (MQ=255)                                                                            aaaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCAcc                                                                      >  2:614427/1‑149 (MQ=255)                                                                             aaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGAc                                                                   >  2:922981/1‑151 (MQ=255)                                                                              aCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGAct                                                                  <  1:714085/151‑1 (MQ=255)                                                                                cGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCTCCGActct                                                                <  2:411914/151‑1 (MQ=255)                                                                                cGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGActct                                                                <  1:866821/151‑1 (MQ=255)                                                                                  ttCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGc                                                              >  2:7666/1‑151 (MQ=255)                                                                                       cTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGt                                                         >  1:571292/1‑151 (MQ=255)                                                                                         aCGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCt                                                       <  1:1402528/151‑1 (MQ=255)                                                                                         aCGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCt                                                       >  1:1087090/1‑151 (MQ=255)                                                                                             cAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTggg                                                    >  1:1016708/1‑150 (MQ=255)                                                                                              aaGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGt                                                   >  1:1387333/1‑150 (MQ=255)                                                                                                gCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATc                                                <  1:495782/151‑1 (MQ=255)                                                                                                 cTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATcc                                               >  1:825620/1‑151 (MQ=255)                                                                                                  tGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATcc                                               >  2:1391841/1‑150 (MQ=255)                                                                                                   ggAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAg                                             <  1:477599/151‑1 (MQ=255)                                                                                                    gAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAgg                                            >  1:485717/1‑151 (MQ=255)                                                                                                     aaCTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGGAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGc                                           >  1:550398/1‑151 (MQ=255)                                                                                                      aCTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGc                                           >  2:315426/1‑150 (MQ=255)                                                                                                        tGCGTGGTCTTGCTGCCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCAtt                                        >  1:1066310/1‑151 (MQ=255)                                                                                                        tGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCAtt                                        >  2:44293/1‑151 (MQ=255)                                                                                                        tGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCAtt                                        >  2:426744/1‑151 (MQ=255)                                                                                                         gCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCAt                                         <  1:1226883/149‑1 (MQ=255)                                                                                                          cGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTg                                      <  2:814468/151‑1 (MQ=255)                                                                                                           gTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTg                                      >  2:216014/1‑150 (MQ=255)                                                                                                           gTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTg                                      >  2:402812/1‑150 (MQ=255)                                                                                                           gTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGa                                     <  1:447751/151‑1 (MQ=255)                                                                                                           gTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGa                                     <  1:1151125/151‑1 (MQ=255)                                                                                                               tCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCg                                 >  2:194302/1‑151 (MQ=255)                                                                                                                cTTGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGGTATCCGCGAACACCCGGTACTGCTGAACCGTGCACTGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCgg                                >  2:123372/1‑151 (MQ=255)                                                                                                                  tGCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGt                               >  1:332032/1‑150 (MQ=255)                                                                                                                   gCTACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGCGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTAc                             >  2:447860/1‑151 (MQ=255)                                                                                                                     tACCACCATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTg                           >  1:990919/1‑151 (MQ=255)                                                                                                                      accaccATTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACt                            <  1:393310/149‑1 (MQ=255)                                                                                                                       ccaccaTTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGAt                         >  1:613306/1‑151 (MQ=255)                                                                                                                       ccaccaTTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGAt                         <  2:1128071/151‑1 (MQ=255)                                                                                                                        caccaTTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATc                        <  1:1109728/151‑1 (MQ=255)                                                                                                                           caTTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGa                      >  2:535001/1‑150 (MQ=255)                                                                                                                            aTTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGa                      >  1:362768/1‑149 (MQ=255)                                                                                                                            aTTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAg                    <  2:239242/151‑1 (MQ=255)                                                                                                                            aTTAAAGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAg                    <  2:948921/151‑1 (MQ=255)                                                                                                                               aaaGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGt                  >  1:1030170/1‑150 (MQ=255)                                                                                                                               aaaGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGt                  <  1:228613/150‑1 (MQ=255)                                                                                                                                 aGCTGCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTaaa               <  2:609789/151‑1 (MQ=255)                                                                                                                                     gCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGc             <  1:643260/149‑1 (MQ=255)                                                                                                                                     gCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCt            >  2:806814/1‑150 (MQ=255)                                                                                                                                     gCGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTa           <  1:194305/151‑1 (MQ=255)                                                                                                                                      cGAAGAAAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTAt          <  1:1193/151‑1 (MQ=255)                                                                                                                                         agaaAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATcc        >  1:1344729/1‑150 (MQ=255)                                                                                                                                         agaaAATGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCa       <  1:122188/151‑1 (MQ=255)                                                                                                                                             aaTGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTAATCGAAGGTAAAGCTATCCAGCTg   <  2:1003101/151‑1 (MQ=255)                                                                                                                                              aTGGGTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGc  >  1:559252/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                                   TCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGC  >  NC_000913/4186406‑4186697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑