Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,097,581	0	A	G	50.0%	73.3 / 20.6	58	noncoding (72/88 nt)	serX	tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (3/26);  new base G (29/0);  total (32/26)
Fisher's exact test for biased strand distribution p-value = 3.30e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTC‑G‑AACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTC‑GGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCG‑CTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAA  >  NC_000913/1097436‑1097731                                                                                                                                                   |                                                                                                                                                            cACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTC‑g                                                                                                                                                        <  2:766666/150‑1 (MQ=31)         tCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTC‑G‑AACCCCCg                                                                                                                                               <  2:134363/150‑1 (MQ=37)           aCGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAGAGGGGGGGGTTT‑T‑AAcccccccc                                                                                                                                             >  2:733684/1‑147 (MQ=255)            cGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTCTG‑AAcccccccac                                                                                                                                            >  2:494371/1‑147 (MQ=255)            cGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTC‑G‑AACCCCCGATAc                                                                                                                                           <  1:549729/151‑1 (MQ=39)               tCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTT‑G‑GAccccccccacgtt                                                                                                                                        >  2:240761/1‑143 (MQ=1)                  gTCCAACCGGCTGTCGCCAGTTGACGAACCCCGGTCGGGGCTTCTCATCCCCCCGGAGCGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAGAGGGGGGGAGTG‑T‑AAccccccacgtggttt                                                                                                                                      >  2:329756/1‑140 (MQ=11)                      aaCTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTT‑G‑AACCCCCCATACggt                                                                                                                                        >  1:882786/1‑142 (MQ=255)                      aaCTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGGGTT‑G‑AAacccccaaacctttaccgct                                                                                                                                 >  1:877473/1‑136 (MQ=1)                         tGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGCGGGGGGGGGGGTC‑C‑AAaccccccaa                                                                                                                                            >  1:383551/1‑133 (MQ=1)                              gTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTC‑G‑AACCCCCGATACGTTGCCGTATACACACt                                                                                                                          <  2:608286/150‑1 (MQ=255)                                cGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTC‑G‑AACCCCCGATACGTTGCCGTATACACACTTTc                                                                                                                       <  1:573639/151‑1 (MQ=255)                                  ccAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTC‑G‑AACCCCCGATACGTTGCCGTATACACACTTTCCa                                                                                                                     <  1:337004/151‑1 (MQ=255)                                          cGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTC‑G‑AACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGc                                                                                                              <  2:176498/150‑1 (MQ=255)                                               cccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTT‑T‑GACCCCCCCTACCTTTCCGTATAAACACTTTTCCCGCGTGCTcc                                                                                                           >  2:824939/1‑148 (MQ=11)                                               cccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGGGGGGGGGGGTT‑T‑AACCCCCCACACGGTTCCGCGTTCACACCTTcccagcg                                                                                                                 >  2:60896/1‑137 (MQ=11)                                                      ggggCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTC‑G‑AACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACt                                                                                                 <  1:733711/151‑1 (MQ=255)                                                            tctcATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTC‑G‑AACCCCCCATACGTTGCCGTATACACACTTTCCACGCGGGCTCCTTCCGCCACTC‑GGac                                                                                           >  1:767532/1‑150 (MQ=17)                                                             ctcATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGGGGGGGGGGTTT‑G‑GAACCCCCCCACGTTTCCGTCTACACACTTTCCCCGCGGGGTCCTTCCGcccc                                                                                                  >  2:1029663/1‑141 (MQ=14)                                                               cATCCCCCCGGTGTGTGCAATATACGAAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTC‑G‑AACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTC‑GGACAcc                                                                                        <  2:667802/151‑1 (MQ=255)                                                               cATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTC‑G‑AACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTC‑GGaca                                                                                          <  2:522275/148‑1 (MQ=255)                                                               cATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGATTC‑G‑AACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTC‑GGacac                                                                                         <  2:675563/148‑1 (MQ=255)                                                                     cccGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGCGAGGGGGGGGATG‑G‑TAACCCCCACACAATGGCGTGTACAAAATTTCCATGAGGGCTCATTCTACCAccctgggccc                                                                                         >  2:523206/1‑135 (MQ=2)                                                                      ccGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATC‑T‑AACCCCCCATACGATTCCTCATACACAATTTTCCAGCGTGTTCCTTCATCCACTC‑GGGCACCT‑ccccc                                                                                 >  2:445969/1‑148 (MQ=17)                                                                       cGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGGGGGGGGGGTTC‑G‑TACCCCCCCTACGTTTCCGTATACCCACTTTCCAGGCGGGGTTCTTCCGCCACCC‑GGACACCC‑CACCcaa                                                                               >  1:660800/1‑150 (MQ=14)                                                                          tgtgtgCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTC‑G‑AAACCCCCATACGTTGCCGGATACAAACCTTTCAGGCGTGGTCCTTCACCCCCCC‑CGACACCT‑CACCCAAATGt                                                                           >  1:556387/1‑151 (MQ=14)                                                                           gtgtgCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTC‑G‑AACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTC‑GGACACCT‑CACCAAATTGt                                                                           <  1:756826/150‑1 (MQ=255)                                                                              tgCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTC‑G‑AAACCCCCCCACGGTTCCGTATACACACCTTTCAGGCGGGCTCCCTCCACCCCCC‑GGACAACTCCCCCAAAttttgtt                                                                        >  1:980300/1‑146 (MQ=9)                                                                               gCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTC‑G‑AACCCCCCATACGTTTCCGGATACACACTTTCCCGGCGGGGTCCTTCAGCCCCTC‑CGACACCC‑CACCCAATTTTCGtt                                                                       >  1:424092/1‑150 (MQ=14)                                                                                cAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAGAGGGGGGGGTTT‑G‑AACCCCCGCTACGTTTTCGGATACACACTTTTCAGGGGGGGGCCTTCAGCCCCCC‑GGACAACT‑CACCaaaattttgtt                                                                       >  2:626776/1‑141 (MQ=14)                                                                                           aaaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTC‑G‑AACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTC‑GGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTg                                                           <  1:761060/151‑1 (MQ=255)                                                                                             aaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTC‑G‑AACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTC‑GGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGa                                                         <  1:106297/151‑1 (MQ=255)                                                                                              aaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTC‑G‑AAACCCCCCTACGTTGCCGTATACACACTTTCCCGGCGTGCTCCTTCAGCCCCCC‑CGGCACCT‑CCCCCAAATTTCGTTCCTGTCtt                                                               >  1:269346/1‑144 (MQ=14)                                                                                               aaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAGAGGGGGGGGTTC‑GTAACCCCCG‑TACATTGCCGTATAAACACTTTCCTGGGGGGGTCCTTCAGCCACCC‑CGACACCC‑CACCCAAATTTTGTttctttctttcttg                                                          >  2:104333/1‑146 (MQ=2)                                                                                                aaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGCGGGGGGGGGATTC‑G‑AAACCCCCCTACGTTTGCGTATATAAACCTTCCCGGCGTGATCCCTTCGACACCC‑CGGCACCT‑CACCCAAATGTTGTTGCTCTCTTGCTgggaa                                                       >  1:280021/1‑147 (MQ=9)                                                                                                  gCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTC‑G‑AACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTC‑GGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAACg                                                      <  2:401399/149‑1 (MQ=255)                                                                                                      gTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTC‑G‑AACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTC‑GGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCG‑Ct                                               <  1:797441/151‑1 (MQ=255)                                                                                                          tttCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTT‑G‑AACCCCCCATACGTTGCCGTATACACACTTTTCAGGCGTGGTCCTTCAGCCACTC‑GGGCACCT‑CACCAAATTTTCGTTCCTGTCTTTCTGGGAAGGGGG‑CTAAtt                                           >  1:394316/1‑151 (MQ=14)                                                                                                          tttCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTC‑G‑GACCCCCGATACCTTGTCGCGTATACACCCTCCAAGGGTGCTCCTTCAGCCACTC‑CGACACCC‑CACCAAATTATCGTTTCTGTCTTTCTGGAGCGGgcg                                                  >  1:97404/1‑145 (MQ=14)                                                                                                           ttCGTACGAGCTCTTCTTTAAATATGGCGGTGTGGGGGGGGTTC‑G‑AACCCCCCATCCGGTGCCGGTTACACCCTTTTCCCGCGCGGTCCTTTTGCCCCTC‑CGGCACCC‑CCCCCAAATGTCGTTTCTTTCTTGTTGGGACGGGGG‑CTAAttt                                          >  1:764440/1‑151 (MQ=9)                                                                                                            tCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTC‑G‑AACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTC‑GGACACCT‑CACCAAATTGTCGTTCCTGTCTGGCTGGAACGGGCG‑CTAAtt                                           <  2:484498/149‑1 (MQ=255)                                                                                                             cGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGATTC‑G‑AACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTC‑GGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCG‑CTAATTTAgg                                       <  2:592312/151‑1 (MQ=255)                                                                                                                aCGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTC‑G‑AACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTC‑GGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCG‑CTAATTTAggg                                      <  1:603693/150‑1 (MQ=255)                                                                                                                aCGAGCTCTTCTTTAAATATGGCGGCGAGGGGGGGGATT‑G‑GACCCCCGCCAAGTTTTCGTATAAACACCTTCCCAGCGCGGTCCTTCAACCACTCGGGACCCCC‑CACCAAATT‑TTGTTCCCGTCTTGCTGGAAAGGGCG‑CTAATTTatg                                       >  1:238820/1‑147 (MQ=9)                                                                                                                  gAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTT‑G‑AACCCCCCATACGTTGCCGGATACACACTTTTCCGGCGGGCTCCCTTAGCCACCC‑GGGCACCC‑CACCAAAATTTTGTTTCTGTTTTTCTGGAACGGGGG‑CTAATTTAGGGaa                                    >  1:263906/1‑150 (MQ=14)                                                                                                                  gAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTT‑G‑AACCCCCCATACGTTTCCGTATACACACTTTTCAGGGGGGGTCCTTCTGCCCCTC‑GGGCACCC‑CACCAAAATTTCGTTTCTGTCTTTCTGGAAAAGGCGCCCAATTTGGGGa                                     >  1:693508/1‑150 (MQ=9)                                                                                                                   aGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTC‑G‑AACCCCCCATACGTTGCCGGATACACACCTTCCAGGCGGGCTCCTTCAGCCCCCC‑GGACACCC‑CCCCCAAATTTCGTTCCTGTCTTTCTGGAACGGGCG‑CTAATTTTTGGaaa                                   >  1:362154/1‑150 (MQ=17)                                                                                                                   aGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTT‑G‑AAACCCCCCTAAGTTGCCGCATACACACTTTCCAGGCGTGCTCCTTCCGCCACTC‑GGACACCT‑CACCCAATTTTCGTTCCTGTCTTTCTTGGAAGGGCG‑CCAAtttttgggaa                                   >  1:33607/1‑143 (MQ=14)                                                                                                                    gCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTC‑G‑AACCCCCCATACGTTGCCGGATACACACCTTCCAGGCGTGCTCCTTCAGCCACCC‑CGACACCC‑CACCCAATTGTCGTTCCTGTCTTTCTGGAACGGGCG‑CTAATTTTGGGaaa                                   >  1:1050391/1‑149 (MQ=17)                                                                                                                         tcttTAAATATGGCGGTGAGGGGGGGATTC‑G‑AACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTC‑GGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCG‑CTAATTTAGGGAAATCAt                               <  1:412255/148‑1 (MQ=255)                                                                                                                         tcttTAAATATGGCGGTGAGGGGGGGATTC‑G‑AACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTC‑GGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCG‑CTAATTTAGGGAAATCATg                              <  1:822347/149‑1 (MQ=255)                                                                                                                            ttAAATATGGCGGTGAGGGGGGGATTC‑G‑AACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTC‑GGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCG‑CTAATTTAGGGAAATCATGAc                            <  1:494387/148‑1 (MQ=255)                                                                                                                              aaaTATGGCGGTGAGGGGGGGATTC‑G‑AACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTC‑GGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCG‑CTAATTTAGGGAAATCATGACCTGa                        <  2:989462/150‑1 (MQ=255)                                                                                                                               aaTATGGCGGTGAGGGGGGGGTTC‑G‑GAACCCCCATACGGTTCCGTATACACACTTTCCAGGCGTGCTCCTTTTGCCACTC‑GGACCACT‑CCACAAAATGTCGTTCCCGTCTTGCTGGAACCGGCG‑CCAATTTAGGGAAAACATGACCTGAgg                      >  2:782421/1‑151 (MQ=18)                                                                                                                                       ggTGAGGGGGGGATTC‑G‑AACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTC‑GGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCG‑CTAATTTAGGGAAATCATGACCTGAGGTcaacaa               >  2:1016862/1‑150 (MQ=255)                                                                                                                                       ggTGAGGGGGGGATTC‑G‑AACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTC‑GGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCG‑CTAATTTAGGGAAATCATGACCTGAGGTCAACaaa              <  2:1045904/151‑1 (MQ=255)                                                                                                                                           aGGGGGGGATTC‑G‑AACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTC‑GGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCG‑CTAATTTAGGGAAATCATGACCTGAGGTCAACAAACttt          <  1:1066171/151‑1 (MQ=255)                                                                                                                                                 ggATTC‑G‑AACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTC‑GGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCG‑CTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGaaa    <  2:228535/151‑1 (MQ=255)                                                                                                                                                   aTTC‑G‑AACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTC‑GGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCG‑CTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGaaaaa  <  1:674174/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                            CACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTC‑G‑AACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTC‑GGACACCT‑CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCG‑CTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAA  >  NC_000913/1097436‑1097731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑