Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,434,861 | 0 | G | T | 47.4% | 58.1 / 35.1 | 57 | P259Q (CCA→CAA) | truA | tRNA pseudouridine(38‑40) synthase |
Reads supporting (aligned to +/- strand): ref base G (7/23); new base T (27/0); total (34/23) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.87e-10 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGC > NC_000913/2434717‑2435011 | cGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGa < 2:299103/150‑1 (MQ=255) cGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGAt < 1:138092/151‑1 (MQ=255) aTGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATc > 1:959036/1‑150 (MQ=255) aTGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCa < 2:913724/151‑1 (MQ=255) ggAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAcc < 1:228238/151‑1 (MQ=255) aaataaatCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATAcccg > 1:665478/1‑149 (MQ=255) aaataaatCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCgg > 1:1019203/1‑151 (MQ=255) taaatCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCgg > 2:497285/1‑148 (MQ=255) cAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTa < 1:506452/151‑1 (MQ=255) aGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTaa < 2:417711/151‑1 (MQ=255) tCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGTCAGGGGAATcc > 1:288175/1‑151 (MQ=255) ccATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATAACCGTCAGGGGAATcc > 2:560870/1‑150 (MQ=255) ccATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCa < 2:637334/151‑1 (MQ=255) atTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCCGGCAGGGGAAaccacccc > 2:650886/1‑149 (MQ=255) atTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACcg < 2:323177/150‑1 (MQ=255) atTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACATTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGAATTCAcc > 1:360379/1‑149 (MQ=255) ttGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATAACCGGCAGGGGAATCCAcccc > 1:412704/1‑147 (MQ=255) tGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGa < 2:62334/150‑1 (MQ=255) ccTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGGTCATACCGGGCAGGGGAAAACACcgcggc > 1:137186/1‑147 (MQ=255) ccTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGATTTTGCAGATCATACCGGGCAAGGTAAACCACCGCGccc > 1:441099/1‑150 (MQ=255) tATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGAACATACCGGGCAGGGGAAACCACCGCGGcc > 2:352727/1‑148 (MQ=255) tGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCCGTCAGGGGAATCCACCGCGACCAGATACAGcc > 1:182054/1‑151 (MQ=255) tGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATAACGGTCAGGGGAATCCACCGCGACCAGATACAGcc > 1:659369/1‑151 (MQ=255) gTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGccc < 2:996048/151‑1 (MQ=255) aCTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACTGCCTGAATTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCt < 1:149586/150‑1 (MQ=255) aTCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGGCAGGGGAATCCACCCCGGCCAGATACAACCCTTCCGCttt > 1:37134/1‑151 (MQ=255) cAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCACTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGt < 2:814721/151‑1 (MQ=255) gCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTtgc < 1:585532/151‑1 (MQ=255) cTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATAACGGTTCGGGGGATACACCCCGACCCGATTAAACCCTTTCCCCTTTTCCggt > 2:695734/1‑146 (MQ=255) cTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGAACATACCGGTCAGGGGAATCCACCGCGGCCCGATAAAACCCCTCCCCTTTTTCCggt > 1:182240/1‑146 (MQ=255) gTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTtgctgc < 2:994044/151‑1 (MQ=255) gTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTtgctg < 1:1096068/150‑1 (MQ=255) tACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGGCAGGGGAATACACCCCGACCAGATACAGCCCCTCCGCCTTTTCCGTTGCTgccccc > 2:1021635/1‑150 (MQ=255) aCGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCa < 1:593697/150‑1 (MQ=255) ctttGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATAACGGGCAGGGGAAACCACCGCGGCCCGATACAACCCTTCCCCTTTTTCCGTTGCTgccgcc > 1:594963/3‑148 (MQ=255) cGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGGCCGGGGAATCCACAGCGACCAAATACACCCCTTCCGCCTTTTCCGTTGCTgccgc > 1:630683/1‑147 (MQ=255) ggCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGAACATAACGGGCAGGGGAATTCCCCGCCACCAGATACAGACCTTCCCCTTTTTCCGTTGCTGCCGCCAGCggt > 1:890077/1‑148 (MQ=255) tACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATAACCGGCAGGGGAATCCACCGCGACCAGATACAGCCCCTCCCCTTTTTCCGTTGCTTCCGCCAGCGTTCTGt > 1:967138/1‑151 (MQ=255) cAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATAATACCCGGCAGGGGAAACCACCGCGACCAGATACACCCCCTCCGCCTTTTCCCTTGCTGCCCCCAGCGGTCt > 2:529871/1‑147 (MQ=255) gAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCCGGCAGGGGAAACCACCCCGACCCGATAAAGCCCCTCCCCTTTTTCCGTTTCTGCCCCCCGCGTTCTGTCCTTTtcc > 1:1024794/1‑151 (MQ=255) gTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTTAAGATCATACCCGTCAGGGGAATCCACCCCGCCCCGATACAGCCCCTCCCCCTTTTCCGTTTCTGCCGCCCGCGGTCTTTCCTTTtccccc > 2:423910/1‑149 (MQ=255) gTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCa < 2:242584/150‑1 (MQ=255) tCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGTAAATCACCCCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCCCCCGCCTTCTGTCCTTTGCCGCCAGc > 1:717560/1‑150 (MQ=255) ccGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCa < 1:736431/150‑1 (MQ=255) cATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATAATACCGGGCAGGGGAATCCACCGCGACCAGATACAACCCCTCCCCTTTTTCCGGTGCTTCCCCCCGCGTTCTTTCCTTTTCCCCCCGcca > 2:959168/1‑144 (MQ=255) attattCTCGTTAGTCCGCCAGAAATAGCGGTCCCATTGGCGGTTTTTGAAGAACATAACAGTGAGGGCAAAACAGCGGGCACAGAAACAGCCTGGTCGCCTTTCCCGGCCCCCCCGCCCGCAGTCTGTCCTTTTccgcccgctccccttc > 1:481321/1‑143 (MQ=255) ttattCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCCGGCAGGGGAATACCCCCCGACCCGATAAAGCCCCTCCGCCTTTTCCGTTGCTGCCGCCAGCGTTCTTTCCCTTTCCGCCCGCAACTCTg > 2:736052/1‑149 (MQ=255) tattCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACAACCCTTCCGCTTTTTCCGGTGCTGCCGCCAGCGGTTTTTCCTTTTCCGCCAGCAACTCTg > 1:78459/1‑148 (MQ=255) cGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCCGCAACTCTGCTATCCAGc > 1:203811/1‑151 (MQ=255) gCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCt < 2:479621/151‑1 (MQ=255) ccAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTg < 2:711395/151‑1 (MQ=255) ccAGAAATAGCGGGCCCATTGGCGGCTTTTGGAGATCATACCCGGCAGGGGAAACCACCCCGACCCCATACAGCCCCTCCCCCTTTTCCGGTGCTGCCCCCCGCGCTTTGTCCTTTTCCGCCCGCAACCCTGCTTTCCCGCTCTcccg > 2:463446/1‑146 (MQ=255) cAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTTCCGTTGCTGCCGCCAGCGGTCTTTCCTTTTCCGCCAGCAACTCTGCTATCCAACTCTCCGGCTgg > 1:31224/1‑151 (MQ=255) ggCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTcc < 1:579932/150‑1 (MQ=255) gTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATc > 2:1063342/1‑149 (MQ=255) tttGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAgg < 1:259078/150‑1 (MQ=255) tGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCt < 2:609673/150‑1 (MQ=255) ggAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGc > 2:422304/1‑151 (MQ=255) | CGATGAGGAAATAAATCAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGC > NC_000913/2434717‑2435011 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |