Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,3440CT47.1% 55.3 / 45.3 69S62F (TCT→TTT) bglJbgl operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (9/27);  new base T (32/0);  total (42/27)
Fisher's exact test for biased strand distribution p-value = 1.86e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGG  >  NC_000913/4604198‑4604490
                                                                                                                                                   |                                                                                                                                                     
cATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttttttt                                                                                                                                                   >  1:1042730/1‑151 (MQ=255)
cATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctt                                                                                                                                                     <  1:88874/149‑1 (MQ=255)
cATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctt                                                                                                                                                     <  2:286914/149‑1 (MQ=255)
cATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctt                                                                                                                                                     <  1:285886/149‑1 (MQ=255)
 aTAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAtttttttttttt                                                                                                                                                  >  1:885814/1‑151 (MQ=255)
        aaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAtttttttttttttttcc                                                                                                                                             >  2:519165/1‑144 (MQ=255)
         aTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATg                                                                                                                                          >  1:304299/1‑151 (MQ=255)
           gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATgag                                                                                                                                        <  1:798748/151‑1 (MQ=255)
           gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATgag                                                                                                                                        <  2:526184/151‑1 (MQ=255)
           gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATgag                                                                                                                                        <  2:618392/151‑1 (MQ=255)
             gTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGcc                                                                                                                                             >  2:167271/1‑144 (MQ=255)
             gTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTGCCATgag                                                                                                                                        >  2:47362/1‑149 (MQ=255)
               cATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGt                                                                                                                                    <  1:10745/151‑1 (MQ=255)
                aTGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTg                                                                                                                                   >  1:443931/1‑151 (MQ=255)
                   agtagtATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgc                                                                                                                                <  2:643116/151‑1 (MQ=255)
                     tagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGGGAAGGGAg                                                                                                                                 >  1:632949/1‑148 (MQ=255)
                     tagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGGAGTGAgcg                                                                                                                               >  2:462506/1‑150 (MQ=255)
                      agtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgcgc                                                                                                                              <  2:974668/150‑1 (MQ=255)
                      agtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCa                                                                                                                             <  2:445186/151‑1 (MQ=255)
                          tCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAggggcg                                                                                                                            >  1:1069372/1‑143 (MQ=255)
                            ggTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGTCCTGAGAAGGGAGGGCCGAgggg                                                                                                                       >  2:665153/1‑151 (MQ=255)
                              tATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTACATTTTTTTTTTTTTGCCCTGTGTAATGAGCCGCGAGAggg                                                                                                                      >  2:62494/1‑150 (MQ=255)
                               aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGa                                                                                                                     <  2:32831/150‑1 (MQ=255)
                               aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGAt                                                                                                                    <  2:363438/151‑1 (MQ=255)
                                ttGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGGGAGCGCAGGGGGGGGtt                                                                                                                   >  2:1102922/1‑151 (MQ=255)
                                  gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTAt                                                                                                                 <  2:659308/151‑1 (MQ=255)
                                   agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTTCCTGAGAAGTGAGGGCAGAGAGGGGTTAt                                                                                                                 >  2:797450/1‑150 (MQ=255)
                                      gTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAGCGGAGAGAGGGATTAtttt                                                                                                              >  2:240708/1‑150 (MQ=255)
                                            tCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGCCATGAGGAGGGAGGGCAGGGAGGGGTTTTCTTTCct                                                                                                          >  1:686331/1‑148 (MQ=255)
                                             cAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAGCGCAGAGAGGGGTTTTCTTTCctgact                                                                                                      >  1:684189/1‑151 (MQ=255)
                                               gAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGCGCAGGGAGGGATTATTTTGCcttac                                                                                                       >  2:210658/1‑148 (MQ=255)
                                                aaaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGaa                                                                                                   <  1:683924/151‑1 (MQ=255)
                                                 aaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGGGCAGAGAGGGATTATCTTGCCTGACTGaa                                                                                                   >  1:250982/1‑150 (MQ=255)
                                                   gTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGGGGGGCAGGGGGGGGTTATCTTTCCTGTCTGAACTg                                                                                               >  2:590106/1‑151 (MQ=255)
                                                      tGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTCTTTTTCTGCCATGAGGAATGTGCGCAGAGAGGGGTTATTTTGGCTGactggactgg                                                                                              >  1:120175/1‑149 (MQ=255)
                                                       gCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTTCCATGAGAAGTGAGCGCCGAGAGGGGTTATCTTGCCTGACTGAACTGGCg                                                                                            >  1:535806/1‑150 (MQ=255)
                                                       gCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGa                                                                                           <  1:1084376/151‑1 (MQ=255)
                                                        cGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTTCCTGACTGAACTGGCg                                                                                            >  1:786674/1‑149 (MQ=255)
                                                              aCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTTCCTGACTGAACTGGGGATTAAGtt                                                                                   >  2:735942/1‑151 (MQ=255)
                                                                 cTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTTCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGATTAAGttt                                                                                  >  2:61952/1‑149 (MQ=255)
                                                                   tataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCcg                                                                              <  2:168865/150‑1 (MQ=255)
                                                                    ataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCcgc                                                                             <  1:776463/150‑1 (MQ=255)
                                                                       aGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCCGAGAGGGGTTATCTTTCCTGACTGAACTGGCGATTAAATTTTCGCGTAc                                                                         >  2:167472/1‑151 (MQ=255)
                                                                       aGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGGAGTGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGATTAAGTTTTCGCGt                                                                           >  1:1033019/1‑149 (MQ=255)
                                                                           ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCAGGGGGGGGTTATCTTGCCTTACTGAACTGGCGATTAAGTTTTCGCGTAccc                                                                       >  2:1033857/1‑149 (MQ=255)
                                                                             aTACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCg                                                                   <  2:526561/151‑1 (MQ=255)
                                                                              tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGGGCGCAGAGAGGGGTTTTTTTGCCTGACCGAACTGGGGATTTAAGTTTCGCGTGccccg                                                                     >  1:566638/1‑146 (MQ=255)
                                                                                       cAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTCTTTTTTTGTCATGTGAAGGGAGCGCAGAGGGGGGCTATCTTGGCCGACTTAACTTGGCCGTTAGTTTACGCGTGCCCGGCGTTTAGTtttt                                                         >  1:845864/1‑151 (MQ=255)
                                                                                            aGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTTCCATGAGAAGGGAGCGCCGAGAGGGGTTATCTTGCCTGACTGAACTGGCGATTAAGTTTTCGCGTACCCCGCGTTTAGTTATTGCgg                                                     >  1:430775/1‑150 (MQ=255)
                                                                                            aGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGga                                                    <  2:1022018/151‑1 (MQ=255)
                                                                                                gTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCAGAGAGGGGTTATTTTTCCTTACTGAACTGGCGATTAAGTTTCCCCCGAACCCGGGGTTTTGTtttttggg                                                     >  2:767961/1‑143 (MQ=255)
                                                                                                gTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgat                                                <  1:400872/151‑1 (MQ=255)
                                                                                                   aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGCCATTGGAAGTGAGCGGGGAGGGGGGTTATCTTGCCTGCCTGGAATGGGGATTTAATTTTCGCGGACCAGGGGTTTGGTtttt                                                         >  2:278566/1‑139 (MQ=255)
                                                                                                   aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTTAGAAGTGAGCGCAGAGGGGGGTTATCTTGCCTGACTGAACTGGCGGTTAAGTTTTCGCGTACCCCGCGGTTTGTTATTTCgg                                                     >  2:124028/1‑143 (MQ=255)
                                                                                                   aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGATTATTTTGCCTGACTGGACTGGGGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGggtggtg                                               >  1:87878/1‑149 (MQ=255)
                                                                                                       cAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTCTGCCATTAGAAGGGAGGGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGATTTAGTTTCCCCGTACCCGGCGTTTAGTTTTTGCGGGTGATGATAtt                                          >  1:659119/1‑150 (MQ=255)
                                                                                                         ggATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGaa                                       <  1:1072669/151‑1 (MQ=255)
                                                                                                          gATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGCCCTGAGAAAGGGGCGCCGAGGGGGGGTATCTTTGCTGACTGAAATGGCGGTTAAGTTTTCCCGTACCCCGCGTTTTGTTATTGCGGGTGATGGTAtt                                          >  2:1081166/1‑147 (MQ=255)
                                                                                                           aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGGGAAGGGAGGGCAGGGGGGGGTTATCTTTCCTGACTGAAATGGCGGTTAAGTTTCCGCGTACCCCGCGTTTAGTTATTGCGGGTGATGATATTGaa                                       >  1:1039386/1‑149 (MQ=255)
                                                                                                                  gTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCt                               <  2:932790/150‑1 (MQ=255)
                                                                                                                  gTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATAATGAAGCTCGGCt                               <  1:400290/150‑1 (MQ=255)
                                                                                                                    cgcgGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGGGGGCCGGGGGGGGTTTTTTTTGCTTACTTAAACGGCGGTTTAATTTTCGCGTACCCGGCGGTTTTGTATTGCGGGTGGTGGTATTTAAGCTCgg                                 >  2:832433/1‑146 (MQ=255)
                                                                                                                    cgcgGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGTTTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGAt                            <  1:184340/151‑1 (MQ=255)
                                                                                                                    cgcgGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGa                             <  2:220935/150‑1 (MQ=255)
                                                                                                                      cgGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTg                          <  2:537326/151‑1 (MQ=255)
                                                                                                                          tctcGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCAGAGGGGGGTTATCTTTCCTGACTGAACTGGGGATTAAGTTTTCGCGTACCCGGCGTTTTGTTATTTCGGATGATGGTATTGAAGCTCGGCTggttggtt                       >  2:969895/1‑150 (MQ=255)
                                                                                                                             cGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCAt                    <  1:580739/150‑1 (MQ=255)
                                                                                                                               tttGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCGGGGGGGGGTTTTTTTGTCTGACTTTAATGGGGGTTAAGGTTCCGCGTACCCCGCGTTTTGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCAtttt                 >  2:1003827/1‑149 (MQ=255)
                                                                                                                                ttGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAATTTTCCGCGTACCCGGCGTTTATTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGt                 <  2:488810/150‑1 (MQ=255)
                                                                                                                                 tGCGGCGGTCATTTTTTCTTTTTTTGCCCTGAGAAGTGAGCGCAGAGAGGGGTTATCTTTCCTTACTGAACTGGCGGTTTAGTTTTCGCGTAACCGGCGTTTAGTTTTTGCGGGTGATGATATTTAAGGTCGGCTGATTTGTTTAtttt                 >  2:269814/1‑147 (MQ=255)
                                                                                                                                  gcggcgGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGc              <  1:234904/151‑1 (MQ=255)
                                                                                                                                     gcggTTATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGAATGAACTGGCGATTAAGTTTCCGCGTGCCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt           <  2:1042693/151‑1 (MQ=255)
                                                                                                                                      cggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCTGCGGTTAGTTATTGCGGATGATGATATTGAAGCGCGGCTGATTGGTTCATTGGCGCCa            >  2:6139/1‑149 (MQ=255)
                                                                                                                                       ggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATc          >  2:867247/1‑150 (MQ=255)
                                                                                                                                        gTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCAc        >  2:1008082/1‑151 (MQ=255)
                                                                                                                                               ttttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTgg  >  2:306285/1‑151 (MQ=255)
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CATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGG  >  NC_000913/4604198‑4604490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: