Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,3500CT49.3% 54.9 / 42.1 69S64F (TCT→TTT) bglJbgl operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (8/27);  new base T (34/0);  total (42/27)
Fisher's exact test for biased strand distribution p-value = 2.59e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGG  >  NC_000913/4604207‑4604490
                                                                                                                                                |                                                                                                                                               
aTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATg                                                                                                                                          >  1:304299/1‑151 (MQ=255)
  gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATgag                                                                                                                                        <  2:526184/151‑1 (MQ=255)
  gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATgag                                                                                                                                        <  2:618392/151‑1 (MQ=255)
  gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATgag                                                                                                                                        <  1:798748/151‑1 (MQ=255)
    gTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGcc                                                                                                                                             >  2:167271/1‑144 (MQ=255)
    gTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTGCCATgag                                                                                                                                        >  2:47362/1‑149 (MQ=255)
      cATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGt                                                                                                                                    <  1:10745/151‑1 (MQ=255)
       aTGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTg                                                                                                                                   >  1:443931/1‑151 (MQ=255)
          agtagtATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgc                                                                                                                                <  2:643116/151‑1 (MQ=255)
            tagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGGGAAGGGAg                                                                                                                                 >  1:632949/1‑148 (MQ=255)
            tagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGGAGTGAgcg                                                                                                                               >  2:462506/1‑150 (MQ=255)
             agtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgcgc                                                                                                                              <  2:974668/150‑1 (MQ=255)
             agtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCa                                                                                                                             <  2:445186/151‑1 (MQ=255)
                 tCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAggggcg                                                                                                                            >  1:1069372/1‑143 (MQ=255)
                   ggTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGTCCTGAGAAGGGAGGGCCGAgggg                                                                                                                       >  2:665153/1‑151 (MQ=255)
                     tATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTACATTTTTTTTTTTTTGCCCTGTGTAATGAGCCGCGAGAggg                                                                                                                      >  2:62494/1‑150 (MQ=255)
                      aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGa                                                                                                                     <  2:32831/150‑1 (MQ=255)
                      aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGAt                                                                                                                    <  2:363438/151‑1 (MQ=255)
                       ttGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGGGAGCGCAGGGGGGGGtt                                                                                                                   >  2:1102922/1‑151 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTAt                                                                                                                 <  2:659308/151‑1 (MQ=255)
                          agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTTCCTGAGAAGTGAGGGCAGAGAGGGGTTAt                                                                                                                 >  2:797450/1‑150 (MQ=255)
                             gTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAGCGGAGAGAGGGATTAtttt                                                                                                              >  2:240708/1‑150 (MQ=255)
                                   tCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGCCATGAGGAGGGAGGGCAGGGAGGGGTTTTCTTTCct                                                                                                          >  1:686331/1‑148 (MQ=255)
                                    cAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAGCGCAGAGAGGGGTTTTCTTTCctgact                                                                                                      >  1:684189/1‑151 (MQ=255)
                                      gAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGCGCAGGGAGGGATTATTTTGCcttac                                                                                                       >  2:210658/1‑148 (MQ=255)
                                       aaaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGaa                                                                                                   <  1:683924/151‑1 (MQ=255)
                                        aaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGGGCAGAGAGGGATTATCTTGCCTGACTGaa                                                                                                   >  1:250982/1‑150 (MQ=255)
                                          gTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGGGGGGCAGGGGGGGGTTATCTTTCCTGTCTGAACTg                                                                                               >  2:590106/1‑151 (MQ=255)
                                             tGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTCTTTTTCTGCCATGAGGAATGTGCGCAGAGAGGGGTTATTTTGGCTGactggactgg                                                                                              >  1:120175/1‑149 (MQ=255)
                                              gCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTTCCATGAGAAGTGAGCGCCGAGAGGGGTTATCTTGCCTGACTGAACTGGCg                                                                                            >  1:535806/1‑150 (MQ=255)
                                              gCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGa                                                                                           <  1:1084376/151‑1 (MQ=255)
                                               cGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTTCCTGACTGAACTGGCg                                                                                            >  1:786674/1‑149 (MQ=255)
                                                     aCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTTCCTGACTGAACTGGGGATTAAGtt                                                                                   >  2:735942/1‑151 (MQ=255)
                                                        cTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTTCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGATTAAGttt                                                                                  >  2:61952/1‑149 (MQ=255)
                                                          tataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCcg                                                                              <  2:168865/150‑1 (MQ=255)
                                                           ataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCcgc                                                                             <  1:776463/150‑1 (MQ=255)
                                                              aGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCCGAGAGGGGTTATCTTTCCTGACTGAACTGGCGATTAAATTTTCGCGTAc                                                                         >  2:167472/1‑151 (MQ=255)
                                                              aGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGGAGTGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGATTAAGTTTTCGCGt                                                                           >  1:1033019/1‑149 (MQ=255)
                                                                  ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCAGGGGGGGGTTATCTTGCCTTACTGAACTGGCGATTAAGTTTTCGCGTAccc                                                                       >  2:1033857/1‑149 (MQ=255)
                                                                    aTACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCg                                                                   <  2:526561/151‑1 (MQ=255)
                                                                     tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGGGCGCAGAGAGGGGTTTTTTTGCCTGACCGAACTGGGGATTTAAGTTTCGCGTGccccg                                                                     >  1:566638/1‑146 (MQ=255)
                                                                              cAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTCTTTTTTTGTCATGTGAAGGGAGCGCAGAGGGGGGCTATCTTGGCCGACTTAACTTGGCCGTTAGTTTACGCGTGCCCGGCGTTTAGTtttt                                                         >  1:845864/1‑151 (MQ=255)
                                                                                   aGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTTCCATGAGAAGGGAGCGCCGAGAGGGGTTATCTTGCCTGACTGAACTGGCGATTAAGTTTTCGCGTACCCCGCGTTTAGTTATTGCgg                                                     >  1:430775/1‑150 (MQ=255)
                                                                                   aGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGga                                                    <  2:1022018/151‑1 (MQ=255)
                                                                                       gTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCAGAGAGGGGTTATTTTTCCTTACTGAACTGGCGATTAAGTTTCCCCCGAACCCGGGGTTTTGTtttttggg                                                     >  2:767961/1‑143 (MQ=255)
                                                                                       gTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgat                                                <  1:400872/151‑1 (MQ=255)
                                                                                          aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGCCATTGGAAGTGAGCGGGGAGGGGGGTTATCTTGCCTGCCTGGAATGGGGATTTAATTTTCGCGGACCAGGGGTTTGGTtttt                                                         >  2:278566/1‑139 (MQ=255)
                                                                                          aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTTAGAAGTGAGCGCAGAGGGGGGTTATCTTGCCTGACTGAACTGGCGGTTAAGTTTTCGCGTACCCCGCGGTTTGTTATTTCgg                                                     >  2:124028/1‑143 (MQ=255)
                                                                                          aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGATTATTTTGCCTGACTGGACTGGGGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGggtggtg                                               >  1:87878/1‑149 (MQ=255)
                                                                                              cAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTCTGCCATTAGAAGGGAGGGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGATTTAGTTTCCCCGTACCCGGCGTTTAGTTTTTGCGGGTGATGATAtt                                          >  1:659119/1‑150 (MQ=255)
                                                                                                ggATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGaa                                       <  1:1072669/151‑1 (MQ=255)
                                                                                                 gATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGCCCTGAGAAAGGGGCGCCGAGGGGGGGTATCTTTGCTGACTGAAATGGCGGTTAAGTTTTCCCGTACCCCGCGTTTTGTTATTGCGGGTGATGGTAtt                                          >  2:1081166/1‑147 (MQ=255)
                                                                                                  aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGGGAAGGGAGGGCAGGGGGGGGTTATCTTTCCTGACTGAAATGGCGGTTAAGTTTCCGCGTACCCCGCGTTTAGTTATTGCGGGTGATGATATTGaa                                       >  1:1039386/1‑149 (MQ=255)
                                                                                                         gTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCt                               <  2:932790/150‑1 (MQ=255)
                                                                                                         gTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATAATGAAGCTCGGCt                               <  1:400290/150‑1 (MQ=255)
                                                                                                           cgcgGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGGGGGCCGGGGGGGGTTTTTTTTGCTTACTTAAACGGCGGTTTAATTTTCGCGTACCCGGCGGTTTTGTATTGCGGGTGGTGGTATTTAAGCTCgg                                 >  2:832433/1‑146 (MQ=255)
                                                                                                           cgcgGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGTTTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGAt                            <  1:184340/151‑1 (MQ=255)
                                                                                                           cgcgGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGa                             <  2:220935/150‑1 (MQ=255)
                                                                                                             cgGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTg                          <  2:537326/151‑1 (MQ=255)
                                                                                                                 tctcGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCAGAGGGGGGTTATCTTTCCTGACTGAACTGGGGATTAAGTTTTCGCGTACCCGGCGTTTTGTTATTTCGGATGATGGTATTGAAGCTCGGCTggttggtt                       >  2:969895/1‑150 (MQ=255)
                                                                                                                    cGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCAt                    <  1:580739/150‑1 (MQ=255)
                                                                                                                      tttGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCGGGGGGGGGTTTTTTTGTCTGACTTTAATGGGGGTTAAGGTTCCGCGTACCCCGCGTTTTGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCAtttt                 >  2:1003827/1‑149 (MQ=255)
                                                                                                                       ttGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAATTTTCCGCGTACCCGGCGTTTATTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGt                 <  2:488810/150‑1 (MQ=255)
                                                                                                                        tGCGGCGGTCATTTTTTCTTTTTTTGCCCTGAGAAGTGAGCGCAGAGAGGGGTTATCTTTCCTTACTGAACTGGCGGTTTAGTTTTCGCGTAACCGGCGTTTAGTTTTTGCGGGTGATGATATTTAAGGTCGGCTGATTTGTTTAtttt                 >  2:269814/1‑147 (MQ=255)
                                                                                                                         gcggcgGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGc              <  1:234904/151‑1 (MQ=255)
                                                                                                                            gcggTTATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGAATGAACTGGCGATTAAGTTTCCGCGTGCCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt           <  2:1042693/151‑1 (MQ=255)
                                                                                                                             cggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCTGCGGTTAGTTATTGCGGATGATGATATTGAAGCGCGGCTGATTGGTTCATTGGCGCCa            >  2:6139/1‑149 (MQ=255)
                                                                                                                              ggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATc          >  2:867247/1‑150 (MQ=255)
                                                                                                                               gTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCAc        >  2:1008082/1‑151 (MQ=255)
                                                                                                                                      ttttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTgg  >  2:306285/1‑151 (MQ=255)
                                                                                                                                                |                                                                                                                                               
ATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGG  >  NC_000913/4604207‑4604490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: