Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,245,234	0	G	T	41.2%	143.9 / 49.6	97	L25F (TTG→TTT)	ycgY	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (9/48);  new base T (40/0);  total (49/48)
Fisher's exact test for biased strand distribution p-value = 8.36e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATT  >  NC_000913/1245087‑1245384                                                                                                                                                    |                                                                                                                                                          cGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGc                                                                                                                                                         >  1:147606/1‑150 (MQ=255)   atatCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTgc                                                                                                                                                      <  2:644428/151‑1 (MQ=255)      tCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTgcg‑c                                                                                                                                                   <  2:726203/150‑1 (MQ=255)       ccGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCG‑Ctt                                                                                                                                                 >  1:117570/1‑151 (MQ=255)        cGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCG‑CTTa                                                                                                                                                <  2:1212442/151‑1 (MQ=255)          aTAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTgcg‑cgtttt                                                                                                                                              >  1:1195420/1‑146 (MQ=255)          aTAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCG‑CTTAtt                                                                                                                                              <  2:1503260/151‑1 (MQ=255)              aGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGGG‑CTTAtt                                                                                                                                              >  2:386344/1‑147 (MQ=255)                tCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTTGG‑CTtttt                                                                                                                                              >  1:1407910/1‑145 (MQ=255)                    ttCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCCTTTTTGaaattt                                                                                                                                       >  1:847200/1‑145 (MQ=255)                     tCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAg                                                                                                                                    <  1:1197800/150‑1 (MQ=255)                      cccGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCG‑CTTTTTTAAAAttt                                                                                                                                      >  1:1526702/1‑147 (MQ=255)                      cccGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGACGGATAGTGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGaa                                                                                                                                  <  1:1210590/151‑1 (MQ=255)                        cGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCG‑CTTATTTAAAATTTATaa                                                                                                                                  >  1:1475403/1‑149 (MQ=255)                        cGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCG‑CTTATTGAACATTTAGaa                                                                                                                                  >  1:422231/1‑149 (MQ=255)                        cGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAt‑t                                                                                                                               <  1:526061/151‑1 (MQ=255)                         ggTCACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAt                                                                                                                                 <  1:805564/149‑1 (MQ=255)                          gTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAT‑Tg                                                                                                                              <  1:1477626/150‑1 (MQ=255)                           tAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCG‑CTTATTGAACATTTAGAAT‑TGAt                                                                                                                            >  2:536094/1‑151 (MQ=255)                            aacaacaacAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCG‑CTTATTTAACATTTAGAAt‑t                                                                                                                               >  2:430787/1‑147 (MQ=255)                            aacaacaacAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATGTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTATAAT‑TGATc                                                                                                                           <  1:1369950/151‑1 (MQ=255)                             acaacaacaGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGGG‑CTTATTGAACAATTAGaa                                                                                                                                  >  2:1415314/1‑144 (MQ=255)                                acaacaGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGGTGCG‑CCTATTGAACATTTAGAAT‑TGATCaa                                                                                                                         >  2:1078986/1‑149 (MQ=255)                                   acaGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCG‑CTTTTTTAAAATTTAGAAA‑TTATCAATCCtt                                                                                                                    >  2:1035792/1‑151 (MQ=255)                                    caGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTc                                                                                                                   <  1:1028493/151‑1 (MQ=255)                                       aaTCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTgg                                                                                                                <  2:576759/151‑1 (MQ=255)                                       aaTCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTgg                                                                                                                <  1:1567525/151‑1 (MQ=255)                                        aTCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGc                                                                                                               <  1:949474/151‑1 (MQ=255)                                         tCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTTCG‑CTTATTTAAAATTTTGAAT‑TTATTAATACTTCttg                                                                                                                >  1:1015893/1‑147 (MQ=255)                                          ccTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGCa                                                                                                              <  2:1006472/150‑1 (MQ=255)                                            ttttttAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGGTGCG‑CTTTTTTAACATTTTGAAT‑TTATCAATCCtt                                                                                                                    >  1:1543522/1‑142 (MQ=255)                                             tttttAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCG‑CTTATTTGAAATTTAGAAT‑TGATCAATCCTTCTTGCAG‑Att                                                                                                         >  2:729413/1‑151 (MQ=255)                                             tttttAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCG‑CGTTTTTAACATTTAGAAT‑TGATCAATCCTTCTGGCAg                                                                                                             >  2:116291/1‑148 (MQ=255)                                             tttttAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑At                                                                                                          <  2:1471584/150‑1 (MQ=255)                                              ttttAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCG‑CTTTTTGAACATTTAGAAT‑TTATCAATTCTTCTGGCAG‑Att                                                                                                         >  1:106393/1‑150 (MQ=255)                                              ttttAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCG‑CTTATTGAACATTTTGAAT‑TGTTTAATCCTTCTTGCAG‑a                                                                                                           >  2:1318907/1‑148 (MQ=255)                                                   attattGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGACa                                                                                                   <  1:1557602/151‑1 (MQ=255)                                                     tattGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCG‑CTTATTGAACATTTAAAAT‑TGATCAAACCTTCTTGCAG‑ATTAAGCCaa                                                                                                  >  2:514144/1‑150 (MQ=255)                                                     tattGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGCCaa                                                                                                  <  2:1567483/150‑1 (MQ=255)                                                      attGTTTCGTTGTTTTTGGCGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGTTGCG‑GTTATTGAACATTTTGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAACCaaa                                                                                                 >  2:1576348/1‑150 (MQ=255)                                                         gTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGGTGCG‑CTTATTTAAAAATTAGAAA‑TTATCAATCCTTCTGGGAG‑ATTAAGCCaaaaaa                                                                                              >  2:1046650/1‑148 (MQ=255)                                                         gTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGGTTTTGCTGCG‑CTTATTTAACATTTTGAAT‑TGATCAAACCTTCTGGGAG‑ATTAAGCCaaaa                                                                                                >  2:1308923/1‑148 (MQ=255)                                                           ttCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCG‑CTTATTTAAAATTTTGAAT‑TGATCAATCCTTCTGGGAG‑ATTAAAGCaaaaaaacc                                                                                           >  2:932567/1‑146 (MQ=255)                                                            tCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGGTTTTGGTTCG‑CTTTTTGTACATTTAGAAA‑TTATCCATCCTTCTGGCAG‑ATTAAGCCAAAATAccc                                                                                           >  2:35872/1‑150 (MQ=255)                                                             cGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGCCAAAATACCCGt                                                                                         <  2:1387379/151‑1 (MQ=255)                                                              gttgttTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCG‑CTTTTTGAAAATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAACCAAAAAAACCGtt                                                                                        >  2:1439523/1‑151 (MQ=255)                                                                tgttTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGCCAAAATACCCGTTc                                                                                       <  1:103323/150‑1 (MQ=255)                                                                 gttTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGCCAAAATACCCGTTCgg                                                                                     <  2:269090/151‑1 (MQ=255)                                                                    tttGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTTCG‑CCTATTTAAAAATTTGAAT‑TTATTAATCCTTCTTGGTG‑ATTAAGCCaaaaaaaccctttgg                                                                                     >  1:949998/1‑137 (MQ=255)                                                                    tttGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGTTGCG‑CATATTTAAAACTTAAAAT‑ACATCAATAATTTCTGCAGAATTAACCCAAAAAAcctttc                                                                                        >  1:1045003/1‑142 (MQ=255)                                                                     ttGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCG‑CTTATTTAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGCCAAAATACCCGTTCGGCaa                                                                                  >  2:68243/1‑150 (MQ=255)                                                                     ttGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCG‑CCTATTGAACATTTAGAAA‑TTATTAATCCTTCTTGCAG‑ATTTAACCAAAAAAACCCTTCGGCaa                                                                                  >  1:162903/1‑150 (MQ=255)                                                                      tGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGAGTTAAAAATGGGGCGGATATTGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGTCAG‑ATTAAGCCAAAATACACGTTCGGCAAt                                                                                 <  1:1184415/150‑1 (MQ=255)                                                                          gtgATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCG‑CTTTTTTAAAATTTAGAAA‑TGAACAAACCTTCCGGGAG‑ATTAAACCCAAAAAACCGCTCCGCAATACtt                                                                             >  1:1409867/1‑150 (MQ=255)                                                                           tgATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCG‑CTTATTTAACATTTAGAAT‑TGATCAAACCTTCTTGCAG‑ATTAAACCAAAAAACCCGTTCGGCAATGCtt                                                                             >  2:996694/1‑149 (MQ=255)                                                                            gATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGCCAAAATACCCGTTCGGCAATGCTTCa                                                                           <  2:1542101/150‑1 (MQ=255)                                                                               tCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCG‑CTTATTTAAAATTTTGAAA‑TTATCAATCCTTTTTGGCG‑ATTTAGCCAAAAAAACCCTTCGGCAATGCTTCAggt                                                                        >  1:471161/1‑148 (MQ=255)                                                                                 aGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGGTTTTGCTGGG‑CCTGTTGAACATTTTTGAT‑TTCTCAATCCCTCTGGGGG‑AATTAGCCAAAATAACCGTTCGGGAATGCCTCAGtt                                                                        >  1:1263328/1‑148 (MQ=255)                                                                                   aaaaTAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGCGCG‑CTTTTTGCAAATTTAAAATATGACAAATCC‑TCTGGCAA‑AATGAGCCAAAAAAAACATTTGGCAACACTTCacgttg                                                                      >  1:1080023/1‑143 (MQ=255)                                                                                   aaaaTAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCG‑CTTATTTAAAATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGCCAAAATAACCGTTCGGCAATGCTTCAGTTAcgcg                                                                   >  2:818242/1‑151 (MQ=255)                                                                                      ataaataTTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCG‑CTTATTAGACATTTTGAAT‑TGTTAAAACCTTCTGGTAG‑ATTAAACCACAATAACCCTTCGGGAAAGCtttacataagcaa                                                                  >  2:1281766/1‑138 (MQ=255)                                                                                        aaataTTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAaga                                                               <  2:820669/150‑1 (MQ=255)                                                                                          atatTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGTTGCG‑CCTATTTAACATTTTAAAT‑TGCTCAAACCCTTTTGCAG‑AGTAAACCAAAATACCCCCTCGGCAAAGATTTCGTTTAGCGAaga                                                               >  2:1420239/1‑148 (MQ=255)                                                                                          atatTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGa                                                            <  2:1316814/151‑1 (MQ=255)                                                                                          atatTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGa                                                            <  1:525510/151‑1 (MQ=255)                                                                                          atatTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGa                                                            <  1:584401/151‑1 (MQ=255)                                                                                           tatTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGGTTTTGGTGCG‑CTTATTTAACATTTTGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAACCAAAATAACCCTTCGGCAATGCTTCTGTTAcgcggaaa                                                               >  1:197712/1‑143 (MQ=255)                                                                                            atTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAAt                                                          <  1:1144119/151‑1 (MQ=255)                                                                                              tttAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCG‑CCTTTTTTAAATTTTGAAT‑TGATTAATCCTTTTGGGCG‑ATTAAGCCAAAAAACCCGTTCGGGAATGCTTTTGTTTCGCGGAGAGGAATgg                                                        >  1:495587/1‑151 (MQ=255)                                                                                                tAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCG‑CTTATTTAACATTTAGAAT‑TTATCAATCCTTCTGGCAG‑ATTAAGCCCAAAAAACCCTTCGGCAATGCTTCAGTTACGCGAAGAGGGATgg                                                        >  2:279255/1‑149 (MQ=255)                                                                                                   aCAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCG‑CTTTTTTAACATTTAGAAA‑TGATCAAACCTTCTTGCAG‑ATTAAGCCAAAATAACCGTTCGGCAATGCTTCAATTACGCGAAGAGGGATGGTCTg                                                    >  2:713313/1‑150 (MQ=255)                                                                                                   aCAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGa                                                   <  1:341722/151‑1 (MQ=255)                                                                                                    cAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGaa                                                  <  1:116277/151‑1 (MQ=255)                                                                                                     aGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCG‑CTTTTTTAAAAATTAGAAT‑TTATTAATACTTCTGGCAG‑ATTAAGCCAAAAAAACCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGaa                                                  >  1:705858/1‑150 (MQ=255)                                                                                                     aGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAAt                                                 <  2:659512/151‑1 (MQ=255)                                                                                                      gTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAAtt                                                <  2:50894/151‑1 (MQ=255)                                                                                                             gTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCtttttt                                         <  1:412085/151‑1 (MQ=255)                                                                                                              tCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCCGCG‑GTTATTTAAAATTTAGAAT‑TGGTTAAACCTTCTTGGAG‑ATTAAGCCAAAAAAACCGTTCGGCAATGCTTTAGTTTCGCGGAGGGGCAAGGGCTGAAATtttttttt                                        >  1:124209/1‑151 (MQ=255)                                                                                                                        aaaaTGGGGCGGATAGTGAGGCGGTTTTTGGTGGG‑CTTTTTGAAAAATTAGAAT‑TGATTAAACCTTTTGGGCG‑ATTTAGCCCAAATAACCGTTTGGCAATGCTTTCGTTATGCGCAGGGGGAAGGGTTGAATTCTTTTTTTGGGTGCt                                 >  1:620352/1‑148 (MQ=255)                                                                                                                        aaaaTGGGGCGGATAGTGAGGCGGTTTTTGCTGCC‑CTTATTTAAAAATTAGAAT‑TTATTAATCCTTTTTGCAG‑ATTTAGCCAAAATAACCCTTCGGCAATGCTTTAGTTTCGCGAAGGGGAATGGGCTGAATTCTTTTTTTTGtttgt                                 >  1:122251/1‑145 (MQ=255)                                                                                                                           aTGGGGCGGATAGTGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATtc                           <  1:429022/151‑1 (MQ=255)                                                                                                                             ggggCGGATAGTGAGGCGGTTTTGGCTGCG‑CTTATTTAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGCCAAAAAACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTTGTTGCTAAATtct                          >  2:482443/1‑150 (MQ=255)                                                                                                                              gggCGGATAGTGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTctct                        <  1:932512/151‑1 (MQ=255)                                                                                                                              gggCGGATAGTGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTctct                        <  2:605584/151‑1 (MQ=255)                                                                                                                               ggCGGATAGTGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTg                       <  1:287748/151‑1 (MQ=255)                                                                                                                               ggCGGATAGTGAGGCGGATTTTGCGGCG‑CTTATTGAACATTTAGAAT‑TGAACAAACCTTCTGGCAG‑AATAAGCCCAAATAACCGCTTGGCAATGCTTCCAGTACGCGAAGAGGGATGGTCTGGAATCTTTTTTTTGTTTCTAAACTttct                        >  1:843665/1‑150 (MQ=255)                                                                                                                                   gATAGTGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAgt                   <  1:1267026/151‑1 (MQ=255)                                                                                                                                       gTGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCt               <  2:517477/151‑1 (MQ=255)                                                                                                                                        tGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCt               <  1:777986/150‑1 (MQ=255)                                                                                                                                         gAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTgg             <  2:1160981/151‑1 (MQ=255)                                                                                                                                         gAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTg              <  1:437651/150‑1 (MQ=255)                                                                                                                                           ggCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGAt           <  2:1518657/151‑1 (MQ=255)                                                                                                                                              ggTTTTGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTAt        <  2:993814/151‑1 (MQ=255)                                                                                                                                                 tttGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTAtgt      <  2:126765/150‑1 (MQ=255)                                                                                                                                                 tttGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTAtgt      <  2:1065147/150‑1 (MQ=255)                                                                                                                                                 tttGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTAtgt      <  2:1441390/150‑1 (MQ=255)                                                                                                                                                   tGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGa    <  2:1015952/150‑1 (MQ=255)                                                                                                                                                    ggCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGatt  >  1:1573546/1‑151 (MQ=255)                                                                                                                                                    ggCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGat   <  2:1549792/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                          CGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCG‑CTTATTGAACATTTAGAAT‑TGATCAATCCTTCTGGCAG‑ATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATT  >  NC_000913/1245087‑1245384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑