Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,619,230	0	T	C	40.9%	147.5 / 13.6	88	I311V (ATT→GTT)	uraA	uracil permease
Reads supporting (aligned to +/- strand):  ref base T (41/11);  new base C (0/36);  total (41/47)
Fisher's exact test for biased strand distribution p-value = 2.85e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTAGCAAACATCGAACGGTGCAGA  >  NC_000913/2619081‑2619375                                                                                                                                                        |                                                                                                                                                  gtAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAAt                                                                                                                                                   <  2:343375/150‑1 (MQ=255) gtAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATc                                                                                                                                                  >  2:170218/1‑151 (MQ=255) gtAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATc                                                                                                                                                  <  1:1469889/151‑1 (MQ=255)   aaTCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATcgc                                                                                                                                                >  2:810148/1‑151 (MQ=255)   aaTCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATcg                                                                                                                                                 <  2:1468454/150‑1 (MQ=255)    aTCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATcgcc                                                                                                                                               <  2:205517/151‑1 (MQ=255)    aTCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATcgcc                                                                                                                                               <  1:992193/151‑1 (MQ=255)           ttcgattcgaTCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATCGccgccccg                                                                                                                                         <  2:484477/150‑1 (MQ=255)            tcgattcgatCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATCGccgccccgc                                                                                                                                        <  2:1193429/150‑1 (MQ=255)               attcgatCAAAACACGAATACTGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGCGATCATCTGGATA‑GCGGCAGACAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATCGGCGCCTCGCCGa                                                                                                                                     >  1:302647/1‑150 (MQ=255)               attcgatCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGAt                                                                                                                                    >  2:123665/1‑151 (MQ=255)               attcgatCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGAt                                                                                                                                    >  1:710419/1‑151 (MQ=255)                  cgatCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGATAAc                                                                                                                                 >  2:381152/1‑151 (MQ=255)                   gatCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGATAAcc                                                                                                                                >  1:1026049/1‑151 (MQ=255)                      cAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGATAAccc                                                                                                                               >  2:283551/1‑149 (MQ=255)                      cAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGATAAccc                                                                                                                               >  2:1210924/1‑149 (MQ=255)                       aaaaCACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGATAACCCAgg                                                                                                                            >  1:584327/1‑151 (MQ=255)                        aaaCACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGATAACCCAGGt                                                                                                                           >  2:310611/1‑151 (MQ=255)                             cGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGATAACCCAGGTACTGt                                                                                                                      >  2:126470/1‑151 (MQ=255)                                  aCCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGATAACCCAGGTACTGTAAac                                                                                                                  >  1:1130763/1‑150 (MQ=255)                                   ccGAAAGCCCCGATGACACAATAAAGCAGCAGCGAACCCCCGCCCACCCCCGACAATGGGATCTTCTGTATA‑GCGGCACCCAGTTT‑CCCGACGCAGGAAAGGAGGAAAGCGAAAACCGCCGCCCCGCCGATAACCCAGGTACTGTAAac                                                                                                                  <  2:1106311/149‑1 (MQ=255)                                     ggAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGATAAGCCAGGTACTGTAAACACgg                                                                                                              >  1:64946/1‑151 (MQ=255)                                        aGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTg                                                                                                           >  2:617774/1‑151 (MQ=255)                                            ccGATGACACCAAAAAGCGGCGGCAAACCCCCCCCCACCCCCGGCAATGGGATCATGGGGATA‑GCGGCAGCCGGTTC‑CCCGACGCAGGAAAGCAGGATAGCAAAAACCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCg                                                                                                       <  2:601780/151‑1 (MQ=255)                                              gaagccacCATAAAGCACCAGCGAACCCCCCCCCCCCCCCGGAAGTGGTATCTTCTGGATA‑GCGGCACCCATTTA‑ACCGACGCAGAAAAGCAGAAAGGCAAAAACCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGa                                                                                                          <  1:1305116/141‑1 (MQ=255)                                              gATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGcc                                                                                                     >  1:1060249/1‑151 (MQ=255)                                               aTGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGcc                                                                                                     >  2:1380208/1‑150 (MQ=255)                                                 gACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATc                                                                                                  >  1:785714/1‑151 (MQ=255)                                                 aacacAAAAAAGCAGCAGAAAAACCCCCCCCATCACGGGAAAGGGATTCTTCTGGATG‑GCGGCAGCAATTTC‑CCCGACCCAGAAAAGCAGGATAGGAAAAACCCCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCAt                                                                                                   <  1:1004177/149‑1 (MQ=255)                                                  acaacaAAAAGCAGCAGCGACCCGCCTCCTACCACCGGCAATGGGGTCATCGGGATA‑GCGGCCCCCATTTA‑GCGGAAGCAGGAAAGCAGAAAAGCGAAAACCCCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCa                                                                                                    <  1:352240/148‑1 (MQ=255)                                                   cacCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCAc                                                                                                >  1:279/1‑151 (MQ=255)                                                       aTAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGcc                                                                                             >  1:1239153/1‑150 (MQ=255)                                                        aaaaGCGGAAGCAAACCCCCCCCCACCCCCGGAAAGGGTATCATCTGGATG‑GCGGCGGCCATTTC‑CCCGACCAAGGAAAGCAGGAAAGCAAAACTCGCCCCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCaa                                                                                           <  2:1568094/150‑1 (MQ=255)                                                             cagcagcGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGttt                                                                                      >  2:1047409/1‑151 (MQ=255)                                                                cagcGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTtct                                                                                   >  2:777021/1‑151 (MQ=255)                                                                     aaccgccgcCCACCCCCGGCAGGGGTATCTTCAGAAGG‑GGGGCGGCCATTTA‑CCCGACGCGGGAAAGAAGAATAGCAAAAGCCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTtct                                                                                   <  1:697114/143‑1 (MQ=255)                                                                     aacccccgccCATCCCCGGAAATGGGATCATCTGGATG‑GCGGCACCCATTTT‑CCCGACGCAGAAAAGCAGGATAGAAAAAACCGCCCCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTtctc                                                                                  <  2:269922/142‑1 (MQ=255)                                                                     aacccccccccACCACCGGAAAGGGGATACTCGGAATA‑GC‑GCCGCCAATTTCCCCGACGCAGAAAAGCAGGATACAAAAACCCCCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTa                                                                              <  1:304154/143‑1 (MQ=255)                                                                     aaaCGCCCCCCACCGCGGAAAATGGGATCAT‑TGGAAAGGGGGCAGCTATTTC‑CCCGACGCAGGAAGGAGGGAGAGGAAAAACCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCg                                                                                <  2:1013920/149‑1 (MQ=255)                                                                      aaCGCCGCCCATCACCGGCAATGGGATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTa                                                                              >  2:941543/1‑150 (MQ=255)                                                                          ccgccCACCCCCGGCAATGGGATCATCTGGATG‑GGGGCAGCCAGTTT‑CCCGACGCAGGAAAGCAGGATAGAAAAACCCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAagt                                                                           <  2:120027/149‑1 (MQ=255)                                                                          ccgccCACCACCGGCAATGGGATCATCTGGATA‑GCGGCAGCCTTTTT‑CCCGACGCAGAAAAGCAGGATAGAAAAAACCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAagta                                                                          <  2:1261971/150‑1 (MQ=255)                                                                          ccccccATCCCCGGAAAGGGGACCATCTGGATA‑GCGGCCGCCAGTTT‑CCCGCCGCAGAAAAGCAGGATAGCAAAAACCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAagta                                                                          <  2:861572/147‑1 (MQ=255)                                                                            gccCATCACCGGCAATGGGATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGATAACCCAGGTTCTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAagtagt                                                                        >  2:927296/1‑150 (MQ=255)                                                                                aTCACCGGCAATGGGATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTg                                                                   >  1:1082699/1‑151 (MQ=255)                                                                                  cgccggaagaggaatcagcTGGATG‑GCGCCAGCACTTTC‑CCCGACGCAGGAAAGCAGAATAGCAAAAACCCCCCCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTg                                                                   <  1:1194019/136‑1 (MQ=255)                                                                                  ccccGGAAATGGGATCATCTGAATA‑GCGGCAGCCATTTT‑CCCGCCGCAGAAAAGCAGGATAGCAAAAACCCCCCCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTg                                                                   <  2:576409/147‑1 (MQ=255)                                                                                   cccGGCAATGGTATCATTTGGATA‑GCGGCAGCCGTTTT‑CCCGAGGCAGGAAAGAAGGATAGCAAAAATCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTgg                                                                  <  2:752170/148‑1 (MQ=255)                                                                                    ccGGCAATGGGATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGt                                                               >  2:968684/1‑151 (MQ=255)                                                                                    ccGGAAATGGAATCATGTGGATA‑GGGGCGGCCATTTT‑CCCGACGAAGAAAAGCAGGATAGAAAAAACCGCCCCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGt                                                               <  2:35248/151‑1 (MQ=255)                                                                                     cGGCAATGGGATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGt                                                               >  2:1280849/1‑150 (MQ=255)                                                                                     cGGCAATGGGATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGt                                                               >  1:1531248/1‑150 (MQ=255)                                                                                         aaggggATCATCTGGATA‑GCGGCGCCCATTTT‑CCCGACGCAGGAAAGCAGGATAGCAAAAATCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGc                                                          <  2:1191776/148‑1 (MQ=255)                                                                                           tGGGATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGcc                                                         >  2:1272284/1‑150 (MQ=255)                                                                                            gggATCATCTGGATG‑GCGGCAGCAAGTTT‑CCCGACGAAGGAAAGCAGGATAGAAAAACTCCCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCa                                                        <  1:1225153/150‑1 (MQ=255)                                                                                            gggATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCa                                                        >  1:990190/1‑150 (MQ=255)                                                                                            gggATCATCTGGAAA‑GCGCCAGCCTTTTT‑CCCGCCGAGGAAAAGCAGGATAGCAAAAACCCCCCCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTg                                                                   <  2:1158373/139‑1 (MQ=255)                                                                                            gggATCATCGGGAGC‑GCGCCACCCATTTT‑ACCGCCGAAGAAAAGCAAGATAGAAAAACCCCCCCCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCaa                                                       <  1:1168223/151‑1 (MQ=255)                                                                                            ggaaccatcGGAATG‑GCGGCAGCCAGTTC‑CCCGACGCGGAAAAGCAGGATAGAAAAAACCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGcc                                                         <  2:834366/144‑1 (MQ=255)                                                                                             gtagaatcTGGAAG‑GCGGAAGCCAGTTA‑ACAGAGAAAGAAAAACGAGAAAGCGAAAACCCCCCCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTg                                                                   <  1:60046/133‑1 (MQ=255)                                                                                                  catctggtggggggcggcCAGTTT‑ACCGACGGAGAAAAGGAGTAGAGGGAAACCCCCCCCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCaaa                                                      <  2:931693/135‑1 (MQ=255)                                                                                                     tGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCAAAGAAGCCgg                                              >  2:751461/1‑151 (MQ=255)                                                                                                     tGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCAAAGAAGCCgg                                              >  2:1100933/1‑151 (MQ=255)                                                                                                      ggATG‑GCGGCAGCCTGTTC‑CCCGACGCAGAAAAGCAGAATAGAAAAAACCGCCCCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCAAAGAAGCCgg                                              <  1:1156800/150‑1 (MQ=255)                                                                                                      ggATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCAAAGAAGCCgg                                              >  1:806210/1‑150 (MQ=255)                                                                                                       gATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCAAAGAAGCCGGa                                             >  2:934243/1‑150 (MQ=255)                                                                                                         atgccGGCCGCCGGTTT‑CCCGACACAGAAAAGGAGAATAGCGAAATCCGCCCCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCAAAGAAGCCGGaa                                            <  1:494698/146‑1 (MQ=255)                                                                                                         atcgCGGCACCAATTTT‑CCCGGCGAAGAAAAGCAGGATAGAAAAACCCCCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCAAAGAAGCCGGaa                                            <  2:710462/147‑1 (MQ=255)                                                                                                         agggCGGCACCCATTTC‑CCCGCCGCAGAAAAGAGGGATAGAAAAAACCCCCCCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCAaagaag                                                  <  2:1419268/141‑1 (MQ=255)                                                                                                              ggcggcCATTTT‑CCCGACGCAGAAAAGCGGGATAGAAAAACCCCCCCCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCAAAGAAGCCGGAAAt                                          <  2:285096/147‑1 (MQ=255)                                                                                                               gccacccgtttccccgccgCAGAAAAGAGGGATAGCAAAACCCCCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCAAAGAAGCCGGAAATCAc                                       <  1:437042/137‑1 (MQ=255)                                                                                                                cAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCAAAGAAGCCGGAAATCACGGt                                    >  1:92280/1‑151 (MQ=255)                                                                                                                   ccAGTTT‑CCCGCCGCAGAAAAGCAGAATAGAAAAAACCGCCCCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCAAAGAAGCCGGAAATCACGGTc                                   <  2:841585/149‑1 (MQ=255)                                                                                                                   ccAGTTT‑CCCGACGCAGAAAAGCAGGATAGCAAAAACCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCAAAGAAGCCGGAAATCACGGt                                    <  2:720528/148‑1 (MQ=255)                                                                                                                     catttccccGACGAAGAAAAGCAGAATAGAAAAACCCCCCCCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCAAAGAAGCCGGAAATCACGGTCGAc                                <  2:92289/144‑1 (MQ=255)                                                                                                                       ttt‑ACCGACGAAGAAAAGCAGGATAGCAAAAATCCCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCAAAGAAGCCGGAAATCACGGTCGACaa                              <  2:720614/150‑1 (MQ=255)                                                                                                                              gACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCAtt                        >  1:1385687/1‑150 (MQ=255)                                                                                                                                      aaaGCGGGATAGCAAAACCCCCCCCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTa                       <  1:729966/143‑1 (MQ=255)                                                                                                                                        aGCAGGATAGCGAAAATCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTAGCAAACATCg             >  2:666553/1‑151 (MQ=255)                                                                                                                                        aGCAGGATAGCGAAAATCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTAGCAAACATCg             >  1:394775/1‑151 (MQ=255)                                                                                                                                        aGCAGGATAGCGAAAATCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACACCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTAGCAAACATCg             >  2:474681/1‑151 (MQ=255)                                                                                                                                            ggagagaaaaaaCCCCCCCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTAGCAAACATCGaa           <  2:578730/141‑1 (MQ=255)                                                                                                                                            ggaaaacaaaaaCCGCCCCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTAGCAAACATCGAACg         <  2:533712/143‑1 (MQ=255)                                                                                                                                             gaaggaaaaaaCCGCCCCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTAGCAAACATCg             <  2:984765/139‑1 (MQ=255)                                                                                                                                              atagaaaaaaCCGCCCCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTAGCAAACATCGAACg         <  2:1557323/143‑1 (MQ=255)                                                                                                                                                   gAAAATCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTAGCAAACATCGAACGGTGc     >  1:1218076/1‑148 (MQ=255)                                                                                                                                                   gAAAATCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCAAAGAAGCAGGAAATCACGGTCGACAACCCATTAGCAAACATCGAACGGTg      >  1:462072/1‑147 (MQ=255)                                                                                                                                                   aaaaaCCCCCCCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTAGCAAACATCGAAc          <  2:1289054/142‑1 (MQ=255)                                                                                                                                                   aaaaaCCCCCCCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTAGCAAACATCGAACGGTGCAGa  <  1:381122/150‑1 (MQ=255)                                                                                                                                                        |                                                                                                                                                  GTAATCCACTTTCGATTCGATCAAAACACGAATACCGGAAGCACCGATGACACCATAAAGCAGCAGCGAAACGCCGCCCATCACCGGCAATGGGATCATCTGGATA‑GCGGCAGCCAGTTT‑ACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTAGCAAACATCGAACGGTGCAGA  >  NC_000913/2619081‑2619375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑