| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 357,890 | 0 | C | A | 49.1% | 44.2 / 35.0 | 55 | L267F (TTG→TTT) | cynR | transcriptional activator of cyn operon; autorepressor |
| Reads supporting (aligned to +/- strand): ref base C (21/7); new base A (0/27); total (21/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.76e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAAT > NC_000913/357744‑358036 | tAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑C‑GCGGCGACTGTCTGCCAGCTATTTTTCCGCCGCAAC‑aaa > 1:1359069/1‑150 (MQ=255) aGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAAcc < 2:158504/151‑1 (MQ=255) gTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAAccgcc < 2:324163/151‑1 (MQ=255) gTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAAccgcc < 2:345420/151‑1 (MQ=255) aGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGt > 1:945795/1‑151 (MQ=255) cgcATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTtc > 1:174578/1‑151 (MQ=255) cccATCAGGCAATCCCAGCCGCAGCCCTGTGTCAGCGCCTACCGTGATTCATTCCCGCCAACACCCGCGCATTCATCCACCGCCAGGTGAAAAAAGGCCTC‑C‑GCGGCGGCTGTCTGCCAGTTTTTTTTCCGCCGCAAC‑AAAAccgccg < 1:545339/146‑1 (MQ=17) ccgtactatcccAGCCGCAGCCCTGTGCCGCCGGCACCGGTGGTTCTTTCCCGCCAAAACCCGCGTTTTTGCCCACCGCCAGGTGAAAAAATGCTTT‑C‑GCGGCGGTTGTCGGCGATTTTTTTTCCCGCCGCAAC‑AAAACCGCCGTtctctc < 1:240265/141‑1 (MQ=255) aaTTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTCCagt > 1:163980/1‑149 (MQ=255) aaTCCCACCCGCAGCCTTTTGCCAGGGCCTCCCTTGATTCTTTCCCCCCACCACCCCCGCTTTTACCAAACGCCATGTGAAAAAATGCCTT‑C‑GGGGCGGCTGTCTGCCAGTTTTTTTCCCCCCGCAAA‑AAAACCGCCGTTCTCTCCagt < 2:1425961/149‑1 (MQ=255) ccacccacAGACTTTTGTCGGCGGCTCCGGTTATTCTTTCCCGCAAACACCCCCGCTTTTACCCACCCCATTGTG‑AAAAATGGCTTGC‑GCGGGGTCTTTCTGCCAGTTTTTTTCCCGCCGCAAA‑AAAACCGCCGTTCTCTCCagta < 2:902377/146‑1 (MQ=255) cgcccgcgaccttgtgTAAGCGGCTCCCGTGTTTCATTCCCCCAACCACCCCCGCTTTTACCCACCCCCATGTAAAAAAATCCCTC‑C‑GCGGCGGCTGCCGGCCAGCTTTTTTCCCCCCGAAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCg < 2:132861/139‑1 (MQ=255) cGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGACGTTCTCTCCAGTAGTGGCggg > 1:1420793/1‑149 (MQ=255) gCAGACCTGTGACAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCgg > 1:219328/1‑147 (MQ=255) cAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCggtg > 2:363351/1‑146 (MQ=255) cAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCgggggg > 1:1321538/1‑148 (MQ=255) aGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCggggg > 1:62728/1‑147 (MQ=255) gACCTGTGCCGGCGCCTCCCGTATTTCTTTCCCCCCAAAACCCGCCCTTTTCCCCACCCCCATTTAAAAAATTCCCTC‑G‑GCGGGGGCTGTCGGCCGGTTTTTTTCCCGCCGCAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCa < 1:580955/148‑1 (MQ=255) ccttgtgTCGGCGCCTCCCTTATTTCTTTCCCCCCAACACCCGCCTTTTTACCCACCCCCTTGTGAAAAAATCCCTC‑C‑GGGGCGGCTTTCTCCAAGTTTTTTTCCCGCCACAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAag < 1:1080564/146‑1 (MQ=255) tgtgTCAGCGACTACCGAGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATCTGCAAAAATGCCTT‑C‑GCGGCGGCCGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGTGcc > 1:157554/1‑143 (MQ=255) tgtCGGCGCCTCCCGTGTTTCTTTCCCGCAAACACCCGCGCTTTTACCCACCCCCTTGTGAAAAAATCCCTC‑C‑GCGGCGGCTTTCTCCCAGTTTTTTTCCCGCCGCAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGaaa < 2:286737/149‑1 (MQ=255) aGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGAGgaaaaa > 2:500210/1‑145 (MQ=255) gCGGCTACGGTTATTCTTTCCCCCAAACACCCGCGCTTTTACCCAACCCCTTGTAAAAAAATCCTTC‑C‑GGGGCGGCTTTCTCCCATTTTTTTTCCCGCCGAAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAAt < 2:1571975/145‑1 (MQ=255) gcctcccttatttCATTCCCCCAAACACCCGCGTTTTTACCCACCCCCATGTAAAAAAATCCCTC‑C‑GGGGCGGCTGTCTCCCAGTTTTTTTCCCCCCGCAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAAt < 2:1337094/132‑1 (MQ=255) acatcgtttCATTCCCCCCAACCACCCCAATTTTCCCCACCCCCATGTAAAAAAATGTTTC‑C‑GGGGCGGCTGTCTTCCACTTCTTTTCCCCCCCCACA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCtt < 2:156423/137‑1 (MQ=255) cGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGcggggggcaaaa > 2:1082606/1‑128 (MQ=255) ttcttccccGCAAACACCCGCGCTTTGCCCCACCGCCATGT‑AAAAAATTCCTT‑CGGCGGCGGTTGTCTGCCAGTTTTTTTCCCCCCAAAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCAt < 1:1425269/142‑1 (MQ=255) ttCTTTCCCACCAACACCCGCGCTTTCACCCACCCCAATGGAAAAAAAGGCTTC‑C‑GCGGCGGCTTCCTGCCATGTTTTTTCCCGCCACAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCAt < 1:66957/149‑1 (MQ=255) ttcccccaaacaccCGCGTTTTTATCCACCGCAATGTCAAAAAATCCTTT‑G‑GGGGGGGCTGTCTGCAAGTTTTTTTCCCGCCGAAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTc < 2:1448017/135‑1 (MQ=255) ccccccAACACCCGCGTTTTTACCAACCCCCATGTGCAAAATGCCCTC‑C‑GCGGCGGCTTTCTGCCGGTTATTTTCCCGCAGCAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTtgtgtg < 2:980333/147‑1 (MQ=255) cccGCCAACACCCGCGCTTTCCCCCAACGCCTTGTGAAAAAAGCCTTT‑C‑GGGGCGGCTTTCGGCCGGTTTTTTTCCGGCCAAAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCa < 1:233096/141‑1 (MQ=255) cccccAACACCCGCGCATTTATCCAACGCCATGTGAAAAAATGCCTC‑C‑GCGGCGGCTGTCTGCCAGTTTTTTTCCCCCCGAAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTtgtg < 2:1025007/145‑1 (MQ=255) ccGCCAACATGGGCACATTTCCCAAACACCATGTGCAAAAACGCCTC‑C‑GCGGCCGTCGCCTGCCAGCT‑TTTTTCCCCCGCCACAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCAt < 2:390182/142‑1 (MQ=255) acaccCGCGTTTTTCCCCAACCCGATGTGAAAAAGCCCCTC‑C‑GCGGCGGCTTTCTGCCAGCTTTTTTCCCGCGGAAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAAt < 2:333236/149‑1 (MQ=255) acCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAAAGAAAAAAATTTTATACCCGCCTGTTGTTTGGGAATTgctg > 2:1187174/1‑151 (MQ=21) gcATTTATCCAACGCCATGTGCAAAAATGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCGAGAGAAAAAAATTTTAAACCCTCATCTTTTGTgggga > 2:606946/1‑137 (MQ=25) aTTTATCCAACGCCATGTGCAAAAATGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCGAGAGCAAAAACTTTTAACCCCCCCCGTTGTTTGGGAATCGCCGCCgg > 2:251574/1‑148 (MQ=25) cAACGCCATGTGCAAAAATGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGAGAAAAAAATTTAAACCCCGCATGTTGTGTGGGAAACGGCGCTGGGAACaa > 2:675516/1‑147 (MQ=37) aaCGCCATGTGCAAAAATGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGAGAAAAAAATTTTATACCCTCCTGTTTTTTGGGGATCGCTGCTGGGAACa > 2:535050/1‑145 (MQ=25) cccATGTGAAAAAATCCCTT‑C‑GCGGCGGCTGTCTGCCATTTTTTTTCCCGCCGCAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGaaa < 2:414859/150‑1 (MQ=255) tgCAAAAATCCCTC‑C‑GGGGCGGCTGTCTGCAAGTTTTTTTCCCGCCGCAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGa < 2:596314/149‑1 (MQ=255) aaaaaTGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCGCGAGAAAAAAATTTTAAACCCTCCTGTTTTGTGGGGATAGCTGCTGGTGAAAAAATGGGAAggggggggg > 2:886000/1‑145 (MQ=255) aaaagatcctcctcggctgCTGTCTGCCTGCTTTTTTCCCGCCAAAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGt < 2:1144083/136‑1 (MQ=255) ggggtcTGTCGCCCCGCTATTTTCCCCCCGCAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGc < 2:837265/143‑1 (MQ=255) gcgccTGCCTGCCATTTTTTTTCCCCCCACAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTcc < 1:890037/150‑1 (MQ=255) cTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAAAAACTTTTATCCCGCCATGTTTTGTGGGAATCCCTGCTGGGTACAATGTGGGAAGGGGAGGGGGGCGGATCaaccccc > 2:360066/1‑141 (MQ=255) tGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCGAGAGAAAAAACTTTAAGACCCTCCTGTTGTGTGGGAATCGCTGCTGGTAACAAAGTGGGAAGGGAAGTGGGGCGAATCAGCCCCAGaa > 1:732710/1‑149 (MQ=255) tCTGCCATTTTTTTTCCCGCCGCAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCa < 2:465013/144‑1 (MQ=255) tCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGTGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACcgc > 2:785024/1‑151 (MQ=255) ccAGTTTTTTTCCCGCCGAAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCgcgc < 2:174599/149‑1 (MQ=255) ttttttcCCGCCGCAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTg < 2:911380/148‑1 (MQ=255) ttttttcCCCCCGCAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACcg < 2:1153709/139‑1 (MQ=255) cccgccgCAAA‑AAAACCGCCGTTCTCTACAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTc < 2:1096199/148‑1 (MQ=255) cccgccgAAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGc < 1:708793/150‑1 (MQ=255) ccgaAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCa < 2:138646/151‑1 (MQ=255) aac‑aaAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAGCCGCGCTAATTGAGTTCGCCTCAAt < 1:997787/149‑1 (MQ=255) | TAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAAT > NC_000913/357744‑358036 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |