Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913366,5260TG44.4% 119.5 / 55.0 99T329P (ACC→CCC) lacIlactose‑inducible lac operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base T (6/49);  new base G (44/0);  total (50/49)
Fisher's exact test for biased strand distribution p-value = 6.44e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

AGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGC  >  NC_000913/366379‑366675
                                                                                                                                                        |                                                                                                                                                     
aGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTGTTGGGGGCCCgggggg                                                                                                                                                     >  1:559752/1‑150 (MQ=255)
aGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGATGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGCTTGCGTTTTTGGCCCCCgggggg                                                                                                                                                     >  2:249189/1‑150 (MQ=255)
  ccTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGttt                                                                                                                                                  <  1:791195/151‑1 (MQ=255)
    tGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGTATTTGGGGCCCGGGGGGgtttt                                                                                                                                                >  2:142320/1‑151 (MQ=255)
       ggTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTACAGTCGGGAAACCTGTCGTGACAGCTGCATTAATGAATCCGCCAACGCGCGGGGAGAGGAGGGTTGTGTAGTTGGCGAACAGGTGGGttttctt                                                                                                                                             >  1:345374/1‑151 (MQ=255)
          gCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTTGGATTTGGGGCCCGGGGGGGttttctttt                                                                                                                                           >  1:406240/1‑150 (MQ=255)
          gCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTttttctttt                                                                                                                                           <  2:1255508/150‑1 (MQ=255)
          gCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTttttctttt                                                                                                                                           <  1:392593/150‑1 (MQ=255)
             tAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGGGGATTTGGGGCCCGGGGGGGTTTTTTTTTCAcc                                                                                                                                       >  2:1106907/1‑151 (MQ=255)
              aaTGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGTGTATTTGGGGCCCGGGGGGTTTTTTTTTTTAcc                                                                                                                                       >  2:242156/1‑150 (MQ=255)
              aaTGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCa                                                                                                                                      <  1:1523136/151‑1 (MQ=255)
               aTGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAg                                                                                                                                     <  1:402014/151‑1 (MQ=255)
                tgagtgagCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGGTTTGGGTGTGGGGGCCCGGGGGGGtttttttttttccc                                                                                                                                       >  2:856335/1‑145 (MQ=255)
                         tAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGGGGCCGGGGGGGTTTTTTTTTTCTCCGGGAGACggg                                                                                                                            >  1:1530938/1‑150 (MQ=255)
                         tAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGGTTTTGGCGCCCGGGGGGGTTTTTTTTTTTACCATGAGACggg                                                                                                                            >  1:1003427/1‑150 (MQ=255)
                          aaCTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACggg                                                                                                                            <  1:1355648/149‑1 (MQ=255)
                          aaCTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGc                                                                                                                           <  1:581203/150‑1 (MQ=255)
                           aCTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGGATTTGGGGCCCGGGGGGGTTTTTTTTTTCCCAATGAGAcag                                                                                                                             >  2:1154072/1‑145 (MQ=255)
                            cTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGTATTTGGGGCCAGGGGGGGTTTTTTTTTTTCCAGGGAGACGGGCaa                                                                                                                         >  2:514727/1‑150 (MQ=255)
                            cTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTTGGCGCCCGGGGGGGTTTTTTTTTTACCAGGGAGACggg                                                                                                                            >  2:1271709/1‑147 (MQ=255)
                              cacaTTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAg                                                                                                                      <  1:53804/151‑1 (MQ=255)
                               acaTTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGc                                                                                                                     <  1:954976/151‑1 (MQ=255)
                                caTTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCt                                                                                                                    <  2:893175/151‑1 (MQ=255)
                                 aTTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTg                                                                                                                   <  1:741271/151‑1 (MQ=255)
                                  ttAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGGTTGGGGATTTGGCGCCCGGGGGGGGTTTTTTTTTACCCGTGGGGCGGGCAAcc                                                                                                                       >  1:1377635/1‑145 (MQ=255)
                                   tAATTGCGTTGCTCTCACTGCCCGCGTGCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCATCGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGa                                                                                                                  <  1:213738/150‑1 (MQ=255)
                                    aaTTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGGATTTGGGGGCCGGGGGGGTTTTTTTTTTTCCCGGGAGACGGGCGACAGCTGAtt                                                                                                                >  1:1126451/1‑151 (MQ=255)
                                     aTTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTg                                                                                                               <  2:1441384/151‑1 (MQ=255)
                                     aTTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTg                                                                                                               <  2:1430013/151‑1 (MQ=255)
                                     aTTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCAAGATGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGGATTGGGCGCCAGGGTGGTTTTTCTTTTCTCCAGTGAGACGGGCAAAAGCTGATTg                                                                                                               <  1:117775/151‑1 (MQ=255)
                                       tgcgttgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTTTTTGGCGCCAGGGGGGGTTTTTTTTTTACCAGTGAGACGGGCAAAAGCTGAtt                                                                                                                >  2:478912/1‑148 (MQ=255)
                                        gcgttgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGGCGTTTTGGGGCCCGGGGGGGTTTTTTTTTTACCCGTGAGACGGGCAACAGCCGACTTccc                                                                                                            >  1:893452/1‑151 (MQ=255)
                                         cgttgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTGGGAGTTGGGGCCCGGGGGGTTTTTTTTTTTACCACTGTGACCGGCcacaaacgattgtc                                                                                                             >  2:735322/1‑139 (MQ=255)
                                         cgttgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGGGCCCGGGGGGGTTTTTTTTTTACCAGTGAGACGGGGAAAAGCTGATTTccc                                                                                                            >  1:418593/1‑150 (MQ=255)
                                         cgttgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCt                                                                                                           <  2:1455832/151‑1 (MQ=255)
                                         cgttgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGCTTGCGGTTTTGGGGCCAGGGGGGGTTTTTTTTTTTCCAGCGAGAAGGGGGACAACTGAttttcc                                                                                                            >  1:458461/1‑146 (MQ=255)
                                          gttgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCtt                                                                                                          <  2:1065438/151‑1 (MQ=255)
                                           ttgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTTGTATTTGGCGGCCGGGGGGGTTTTTTTTTTACCCGGGAGACGGGCCACAGCTGATTGcccct                                                                                                          >  2:360095/1‑148 (MQ=255)
                                           ttgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGTGTTTGGGGCCAGGGGGGGTTTTTTTTTTACCAGTGAGACGGGGAACAACTGATTGccc                                                                                                            >  2:621347/1‑148 (MQ=255)
                                           ttgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGTATTTGGCGCCCGGGGGGGTTTTTTTTTCACCAGTGGGAAGGGCAACAACTGATTGcccct                                                                                                          >  2:1079245/1‑148 (MQ=255)
                                           ttgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGCTTGCGTATTGGGGGCCCGGGGGGGTTTTCTTTTCACCAGGGAGACGGGCAACAGCTGATTGcccct                                                                                                          >  1:1464734/1‑148 (MQ=255)
                                            tgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGTATTTGGGGCCCGGGGGGTTTTTTTTTTTTCCAGTGAGACGGGGAACAACTGATTGccc                                                                                                            >  2:1443468/1‑147 (MQ=255)
                                              cgcTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGTTTTGGGGGCCCGGGGGGGTTTTTTTTTTACCAGCGAGACGGGCAACAGCTTATTTCCCTTTAcc                                                                                                      >  2:135978/1‑151 (MQ=255)
                                              cgcTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCAcc                                                                                                      <  1:455395/151‑1 (MQ=255)
                                               gcTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGTATTGGGGGCCCGGGGGGGTTTTTTTTTTTCCAAGGAGACGGGCCACAGCTGAATGCCCTTCAcc                                                                                                      >  2:860555/1‑150 (MQ=255)
                                                  cACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCt                                                                                                  <  1:1407163/151‑1 (MQ=255)
                                                       cccGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGGATTTGGGGCCAGGGGGGGTTTTCTTTTTACCCGGGGGACGGGGAACAACTGAGTGCCCCTCACCCCCTCGccc                                                                                             >  1:6076/1‑151 (MQ=255)
                                                        ccGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCt                                                                                            <  1:47562/151‑1 (MQ=255)
                                                         cGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTCTTTGGCGGCAGGGGGGGTTTTTTTTTTACCCGCGAGACGGGCAAAAGCTGATTGCCCCTCACCGcc                                                                                                   >  1:1029207/1‑143 (MQ=255)
                                                          gCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGTGGATTTGGGGCCCGGGGGGTTTTTTTTTTTTCCAGGGAGACGGGGAACAGCTGATTGCCCTTTACCCCCTGGccc                                                                                             >  2:59980/1‑148 (MQ=255)
                                                            tttCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGGTTTTGTTGTTGGGGGCCGGGGGGGTTTTTTTTTTTCCCAAGGGGAGGGGAAAAGCTGGTTGCCCCTCTCCGCCCCGCCCCgag                                                                                         >  1:1283126/1‑150 (MQ=255)
                                                               ccAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTgagaga                                                                                      <  1:1485446/150‑1 (MQ=255)
                                                                 aGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTTGCCCTGAGAGAGt                                                                                    <  1:676901/150‑1 (MQ=255)
                                                                  gTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTg                                                                                  <  1:1281354/151‑1 (MQ=255)
                                                                  gTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTg                                                                                  <  1:1579609/151‑1 (MQ=255)
                                                                        aaaCCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTTGTATTTGGCGCCAGGGGGGGTTTTTTTTTTACCAGTGAGACGGGCAACAGCTGATTGCCCCTCACCCCCCGGCCCTGagagaggt                                                                                   >  2:898117/1‑142 (MQ=255)
                                                                        aaaCCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGTATTTGGGGCCCGGGGGGGTTTTTTTTTTACCAGGGAGACGGGGAAAAGCTGGTTTCCCCTCACccccccggcccggggg                                                                                       >  2:1304434/1‑128 (MQ=255)
                                                                           ccTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCg                                                                         <  1:253929/151‑1 (MQ=255)
                                                                             tGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGCTTGCGCATTTGGGGCCCGGGGGGGTTTTTTTTTTACCCCGGTGGAGGGCAACAACCGGTTGCCCCTCTCCCCCCCGGCCTCGGAGAGGTGTTGCAAGccgg                                                                       >  1:1177483/1‑148 (MQ=255)
                                                                                 gTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGTATTTGGGGCCCGGGGGGGTTTTTTTTTTACCAGTGAGAAGGGGAACAGCTGATTGCCCTTCACCCCCTGGCCCTGAGAGAGGTGCAGCAAACGGGcc                                                                     >  2:1525302/1‑149 (MQ=255)
                                                                                 gTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCAc                                                                   <  1:1579776/151‑1 (MQ=255)
                                                                                   gCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGc                                                                 <  1:670313/151‑1 (MQ=255)
                                                                                   gCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGc                                                                 <  2:674028/151‑1 (MQ=255)
                                                                                    ccAGCTGCATTAAGGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGAGTATAGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTTCACGCt                                                                <  1:1152088/151‑1 (MQ=255)
                                                                                       gCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTCTTTGGGGCCAGGGGGGGTTTTCTTTTTACCACAGAGAGGGGCAACACCGGATTGGCCCTCCCCCCCCCGCCCTTGGAGAGTTGCCGCAAGCGGGCCACCCttg                                                              >  1:464665/1‑148 (MQ=255)
                                                                                       gCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTgg                                                              <  1:59082/150‑1 (MQ=255)
                                                                                         tGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGTGTATTTGGCGGCAGGGGGGGTTTTTTTTTTAACAGAGAGACGGGGAAACACTGGTTGACCTTCACCGCCCGGCCCCGAGAGAGTTGGGGCAAGCGGGGCACGCCGGgtt                                                           >  2:860737/1‑151 (MQ=255)
                                                                                          gCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGttt                                                           <  2:6076/150‑1 (MQ=255)
                                                                                           cATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTTGGCGCCCGGGGGGGTTTTCTTTTTACCAGTGAGACGGGCAACCGCTGATTGCCCTTCACCCCCCGGCCCCGCGAGAGTTGCAGCAAACGGTCCACGCTGGttt                                                           >  1:899523/1‑149 (MQ=255)
                                                                                                aTGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGTAGCAAGCGGTCCACGCTGGTTTGCCCcag                                                    <  2:345403/151‑1 (MQ=255)
                                                                                                   aaTCGGCCAACGCGCGGGGAGAGGCGGTTTTCGTATTTGGCGCCCGGGGGGGTTTTTTTTTTTCCAGGGAGACGGGCAACAACTGATTTCCCTTCACCCCCCGGCCCTGAGAGAGTTGCAGCAAGCGGGCCACGCTGGGTTTcccc                                                      >  2:135067/1‑146 (MQ=255)
                                                                                                   aaTCGGCCAACGCGCGGGGAGAGGCGGTTTTCGCATTTGGGGGCCGGGGGGTTTTTTTTTTTAACCGCGGGAGCGGCAAAACCTGGTTTCCCCTCACCGCCCCGCCCCCAGGGAGATGGACCACGAAGTCCCCGCCGGCTTGaccccccgg                                                 >  2:1374923/1‑146 (MQ=255)
                                                                                                   aaTCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTTATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCcagcag                                                 <  2:1135718/151‑1 (MQ=255)
                                                                                                   aaTCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCcagcag                                                 <  2:1204148/151‑1 (MQ=255)
                                                                                                    aTCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTTGGGGCCAGGGGGGGTTTTTTTTTTACCAGTGAGACGGGGAACAGCTGATTGCCCTTCACCCCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCCGCAgg                                                >  2:1303603/1‑151 (MQ=255)
                                                                                                    aTCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAgg                                                <  1:1357499/151‑1 (MQ=255)
                                                                                                      cGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGc                                               <  1:1063203/150‑1 (MQ=255)
                                                                                                      cGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGc                                               <  2:1099825/150‑1 (MQ=255)
                                                                                                         ccAACGCGCGGGGAGAGGCGGTTTTCGTATTGGGGGGCCGGGGGGTTTTTTTTTTTACCCGGGAGACGGGGAACAGCCGATTGCCCTTCACCCCCTGGCCCTGAGAGAGATGCGGCAAGCGGTCCACCCTGGTTTGCCCCCGCAgg                                                >  2:725288/1‑146 (MQ=255)
                                                                                                         ccAACGCGCGGGGAGAGGCGGTTTGCGTATTTGGCGCCAGGGGGGGTTTTCTTTTTACCAGGGAGACGGGCAACAGCTGATTGCCCTTCACCCCCCGGCCCCGAGAGAGTTTCAGCAAACGGTCCCCCCTGGTTTGCCCCCGCAGGGGaa                                            >  2:225834/1‑150 (MQ=255)
                                                                                                             cgcgcgGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTCGCCCCAGCAGGCGAAAAtac                                       <  2:652588/151‑3 (MQ=255)
                                                                                                                   gggAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCGCCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTg                                 <  1:9364/151‑1 (MQ=255)
                                                                                                                       gagGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGAtgg                             <  1:1536049/151‑1 (MQ=255)
                                                                                                                        agGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGAtgg                             >  1:1164495/1‑150 (MQ=255)
                                                                                                                         ggCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGAtggtg                           >  2:1166267/1‑151 (MQ=255)
                                                                                                                          gCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGAtggtgg                          >  2:854476/1‑151 (MQ=255)
                                                                                                                          gCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGAtggtgg                          <  2:1531030/151‑1 (MQ=255)
                                                                                                                           cGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATggtggt                         <  1:803441/151‑1 (MQ=255)
                                                                                                                             ggTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGtt                        <  1:656189/151‑1 (MQ=255)
                                                                                                                                       aTTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCggg               >  2:813005/1‑151 (MQ=255)
                                                                                                                                       aTTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGAAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAAcggcgg                <  2:634956/150‑1 (MQ=255)
                                                                                                                                             ggCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGatat           <  1:409864/151‑1 (MQ=255)
                                                                                                                                              gcgcCAGGGTGGTTTTTCTTTCCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGAtata          <  1:414146/151‑1 (MQ=255)
                                                                                                                                               cgcCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATaa         <  2:1536977/151‑1 (MQ=255)
                                                                                                                                                   cAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACAt      <  2:1242851/151‑1 (MQ=255)
                                                                                                                                                    aGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACAt      >  2:1157261/1‑150 (MQ=255)
                                                                                                                                                      ggtggtTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAg   <  2:371304/151‑1 (MQ=255)
                                                                                                                                                       gtggtTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGGTATAAAATGAg   >  1:742897/1‑150 (MQ=255)
                                                                                                                                                       gtggtTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGa    >  2:517573/1‑149 (MQ=255)
                                                                                                                                                       gtggtTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGc  <  2:921349/151‑1 (MQ=255)
                                                                                                                                                        |                                                                                                                                                     
AGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGC  >  NC_000913/366379‑366675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: