Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009135,1590GA44.1% 96.1 / 63.6 93intergenic (+139/‑75)thrC/yaaXL‑threonine synthase/DUF2502 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (47/5);  new base A (0/41);  total (47/46)
Fisher's exact test for biased strand distribution p-value = 3.20e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGG  >  NC_000913/5010‑5306
                                                                                                                                                         |                                                                                                                                                   
atcatcAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGgaga                                                                                                                                                    <  2:442087/151‑1 (MQ=255)
   atcaGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAAt                                                                                                                                                  >  1:1428506/1‑150 (MQ=255)
   atcaGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATa                                                                                                                                                 <  2:505946/151‑1 (MQ=255)
       gTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATaacaa                                                                                                                                             <  1:1183347/151‑1 (MQ=255)
         aaaaTCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAAcc                                                                                                                                           >  1:1035059/1‑151 (MQ=255)
         aaaaTCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAAcc                                                                                                                                           >  1:788251/1‑151 (MQ=255)
         aaaaTCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAAcc                                                                                                                                           <  1:864485/151‑1 (MQ=255)
           aaTCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAAccgc                                                                                                                                         >  2:621453/1‑151 (MQ=255)
               tATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGtt                                                                                                                                     >  1:216512/1‑151 (MQ=255)
                aTTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGtt                                                                                                                                     >  2:46895/1‑150 (MQ=255)
                 ttCATTATCTCAATCAGGCCGGTTTTGCTTTTAGGCAGCCCGGCTTTTTTATGAAGAAATTATGGAAAAAAAGGACGGGAAAAAAGGAAAAATCCGCAATAAATGGGATAACTTAGAGATTAGGATGGCGGAGAAAAACACccgccg                                                                                                                                       <  1:1342035/147‑1 (MQ=255)
                   cATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTtctc                                                                                                                                  >  1:1493422/1‑150 (MQ=255)
                        tctcAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGt                                                                                                                                      >  2:1511781/1‑141 (MQ=255)
                           cAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTa                                                                                                                          >  1:444806/1‑150 (MQ=255)
                            aaTCGGGCGGGTTTTGTTTTTATGCAGCCCGTTTTTTTTATAAAAAAATTAGGAAAAAAAATAACAGGAAAAAAGAAAAAATTCACAAAAAATGCGGTAACTTAGAGATGAGGATGGGGGAAAAAAACAACCGCCGTTCTCATCg                                                                                                                              <  2:824577/145‑1 (MQ=255)
                            aaTCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAAt                                                                                                                        >  1:680461/1‑151 (MQ=255)
                                    cGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGat                                                                                                                >  1:105580/1‑151 (MQ=255)
                                     gggTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGat                                                                                                                >  2:1063401/1‑150 (MQ=255)
                                        tttGTTTTTATGCAGCCGGGTTTTTTTAAAAAAAAATTAGGGAAAAAAAGGAAAGGAAAAAAGGAAAAATTCTAAATAAAGGCGTTAACTTAGAGATTAGGATGGGGGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATc                                                                                                             <  2:479637/150‑1 (MQ=255)
                                             ttttgagCAGCCCGGTTTTTTTATAAAGAAATTAGGAAAAAAAAGGACAGGAAAAAAGAAAAAATTCTAAAAAAAGGCGGAAACTTGGAGTTTAGAATTGGGGAGAAAAACATCCGCCGTTCTCATCGAGTAATCTCCGGATATCg                                                                                                            <  2:1064461/140‑1 (MQ=255)
                                             ttttATCCGGCCGGTTTTTTTTAAAAAAAAATTAGGGAAAAAAAGGAAAGGAAAAAAGGAAAAATTCTAAAAAATGGCGTAAACTAAGAATTTAGGTTTGGGGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGAcc                                                                                                         <  1:894012/149‑1 (MQ=255)
                                              tttATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCAt                                                                                                      >  2:476068/1‑151 (MQ=255)
                                               ttAGGCAGCCCGTTTTTTTTAGGAAAAAATTATGGAAAAAAATGACGGGAAAAAAGGAAAAATTCAAAAAAAATGGGTTAATTTAGAGATTAGGATGGGGGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCAt                                                                                                      <  2:1149252/150‑1 (MQ=255)
                                                    cAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACggg                                                                                                >  1:929396/1‑151 (MQ=255)
                                                       cccGGTTTTTTAATAAAAAAATAATAGAAAAAAATGAGAGGAAAAAAGGAAAATTTCTAAAAAAGGGGGATATTTAAGAAATGAGATTTGGGGAAAAAAACAGCCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCa                                                                                              <  1:98516/150‑1 (MQ=255)
                                                        ccGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAAt                                                                                            >  1:48851/1‑151 (MQ=255)
                                                         cGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATg                                                                                           >  1:1145682/1‑151 (MQ=255)
                                                            cTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGa                                                                                          >  1:1416027/1‑149 (MQ=255)
                                                            cTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGAt                                                                                         >  1:367234/1‑150 (MQ=255)
                                                             ttttttATGAAGAAATTATGAAAAAAAATGACAGGAAAAAAGGAGAAATTCTAAATAAATGCGTTAACTTAGAGATTAGGATTGCGAAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGAt                                                                                         <  2:849194/149‑1 (MQ=255)
                                                              tttttATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATaa                                                                                       >  1:1420146/1‑150 (MQ=255)
                                                                  tATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAgg                                                                                   >  2:599367/1‑150 (MQ=255)
                                                                   aTGAAAAAATTAGGGAAAAAAATGAAAGGAAAAAAGAAAAAATTCTAAATAAAGGCGGTAACTTAGAAATTGGGATTGCGGAAAATAAAAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAgg                                                                                   <  1:766097/149‑1 (MQ=255)
                                                                    tGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGt                                                                                >  2:1513418/1‑151 (MQ=255)
                                                                      aaaaaatgaggaaaaaaaaGGAAAGGAAAAAAGGAAAAATTCTAAAAAAATGGGTTAATTTAGAGATTGGGTTGGGGGAAAAAAAAAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTaa                                                                              <  1:751622/137‑1 (MQ=255)
                                                                      aaaaaaTTAGGGAAAAAAATGACGGGAAAAAAGGAAAAATTCAAAAAAAAGGCGGTAACTAAGAGATTAGGATGGCGGAAAAAAAAAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAgg                                                                                   <  2:1371748/143‑1 (MQ=255)
                                                                      aaaaaaTTAGGGAAAAAAATGACAGGAAAAAAGGAAAAATTCTAAAAAAAGGCGGTAATTAAGAGATGAGGATTGGGGAAAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAg                                                                                 <  2:1360885/145‑1 (MQ=255)
                                                                      aaaaaaTTAGGGAAAAAAATGAAAGGAAAAAAGGAAAAATTCTAAAAAAAGGCGGTAATTTAGAGATAAGGATGGGGAAAAAAAAAAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAg                                                                                 <  1:536122/145‑1 (MQ=255)
                                                                      aaaaaaTTAGGAAAAAAAAGAAAAGGAAAAAAGGAAAATTTCTAAAAAAAGGGGGTAACTAAGAGATTAGGATTGCGGAAAAAAAAACCCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTaa                                                                              <  1:309210/148‑1 (MQ=255)
                                                                       aaaatttAGGGAAAAAAAGGAAGGGAAAAAAGGAAAAATTCTCAAAAAAGGCGGTAACTTAGAGTTTGGGATGGGGAAAAAAAAAAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAg                                                                                 <  2:774229/142‑1 (MQ=255)
                                                                       aaaaaaTAAGGAAAAAAAAGAAAAGGAAAAAAGAAAAAATCCTAAAAAAGGGCGTTAATTTAGATATAAGGATTGGGAAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGt                                                                                <  1:1166512/147‑1 (MQ=255)
                                                                          aaTTAGGGAAAAAAATGAAAGGAAAAAAGGAGAAATTCTAAATAAATGCGGTATTTTAGAGATTAGGATGGGGGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCtg                                                                          <  1:623383/151‑1 (MQ=255)
                                                                          aaTTAGGGAAAAAAAGGACGGGAAAAAAGGAAAAATTCTAAATAAATGCGGTAACTTAGATATTGGGGTGGGGAAAAAAAAAAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCtg                                                                          <  2:1056887/151‑1 (MQ=255)
                                                                            ttATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCtgtg                                                                        >  1:241540/1‑151 (MQ=255)
                                                                             tATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCtgtg                                                                        >  1:1464187/1‑150 (MQ=255)
                                                                             tATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCtgtg                                                                        >  2:19826/1‑150 (MQ=255)
                                                                              agggAAAAAAATGACAGGAAAAAAGGAAAAATTCACAAAAAATGCGTTAATTTAGAGATTAGGTTTGGGGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCtat                                                                         <  2:261396/146‑3 (MQ=255)
                                                                                gaaaaaaaaTGACAGGAAAAAAGAAAAAATTCAAAAAAAAGGCGTAATCTAAGAAATTAGGATGGGGAAAAAAAAAAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGa                                                                       <  1:928545/144‑1 (MQ=255)
                                                                                  aaaaaaaTGACAGAAAAAAAGGAAAAATTCTCAATAAAGGCGGTAACTTAGAGATTAGGATGGGGGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCtgtg                                                                        <  2:1032573/143‑1 (MQ=255)
                                                                                   gaAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGaaaa                                                                    >  1:819876/1‑148 (MQ=255)
                                                                                    aaaaaaaagaaGGAAAAAAAGAAAAATTTCAAAAAAAAGGGGTTATTTAAGATATTAGGTTTGGGGAAAAAAAAAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCtgt                                                                         <  1:1061616/134‑1 (MQ=255)
                                                                                     aaaaTGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATg                                                               >  2:306567/1‑151 (MQ=255)
                                                                                       aaTGACAGGAAAAAAGGAAAAATTCTAAATAAATGCGTTAACTTAGAGATTAGGATTGGGGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCa                                                             <  2:1246816/151‑1 (MQ=255)
                                                                                               ggaaaaaaGGAAAAATTCTAAAAAAAGGGGTTATTTTAGAAATTAGGTTGGGGGAAAAAAAAAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATc                                                      <  2:444870/148‑1 (MQ=255)
                                                                                                 gAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGt                                                    >  1:513151/1‑151 (MQ=255)
                                                                                                  aaaagGGAAAAATTCTAAAAAAAGGCGGAAACTGAGAAATTAGGATGGGGGAAAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTAt                                                       <  2:149034/147‑1 (MQ=255)
                                                                                                  aaaaaGGAAAAAATTCTAAAAAAATGCGTTAATTTAGAAATTGGGATTGGGAAAAAAAAAAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGt                                                    <  2:840842/151‑1 (MQ=255)
                                                                                                  aaaaaGGAAAAATTCAAAAAAAATGCGGTAACTAAGAGATAGGGATGGGGGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTa                                                   <  2:376607/151‑1 (MQ=255)
                                                                                                  aaaaaGGAAAAATTCAAAAAAAAGGCGGTAATTTAGAGATTAGGTTGGGGGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGt                                                    <  1:519085/150‑1 (MQ=255)
                                                                                                  aaaaaGAAAAAATCCTCAATAAATGAGTTAACTAAGATATTAGGATGGGGGAAAAAAACAGCCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCaa                                                            <  1:1247344/142‑1 (MQ=255)
                                                                                                   aaaaGAAAAAATTCTCAATAAAGGCGGTAACTTAGAGATTGGGATTGCGGAAAAAAAAAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTAc                                                  <  2:1285555/151‑1 (MQ=255)
                                                                                                    aaaGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACt                                                 >  2:671719/1‑151 (MQ=255)
                                                                                                      aGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCg                                               >  1:379383/1‑151 (MQ=255)
                                                                                                           aaaaTTCAAAAAAAATGCGTTAACTTAGAGATTAGGATTGGGGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCaat                                           <  2:317910/150‑3 (MQ=255)
                                                                                                            aatttCACAAAAAAGGCGGTAACTTAGAAATTAGGATGGGGGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACtt                                          <  2:124159/148‑1 (MQ=255)
                                                                                                            aaaTTCTCTATAAAGTAGTTCACTTAAAGATTAGGTTTGCGAAAAAAAACACCCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACt                                           <  1:468547/150‑1 (MQ=255)
                                                                                                            aaaTTCTCAAAAAATGGGGTAACTAAGAGATTAGGTTTGCGAAAAAAACCATCCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACtt                                          <  2:491366/151‑1 (MQ=255)
                                                                                                             attcctaaaTAAAGGCGTTAATTAAGAATTTGGGTTGGGGAAAAAAAAAAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACttt                                         <  1:739990/144‑1 (MQ=255)
                                                                                                               ttCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTcc                                       >  1:525146/1‑151 (MQ=255)
                                                                                                                 ctcAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCt                                     >  2:886203/1‑151 (MQ=255)
                                                                                                                 ctcAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCt                                     >  1:864853/1‑151 (MQ=255)
                                                                                                                    aaTAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGt                                  >  2:569675/1‑151 (MQ=255)
                                                                                                                     aaaaaTGCGGAATTTTAGAGATTAGGATGGGGAAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGtt                                 <  2:216536/149‑1 (MQ=255)
                                                                                                                     aTAAATGGGGAAACTTAGAAATAAGGATTGGGGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGtt                                 <  2:536955/151‑1 (MQ=255)
                                                                                                                     aTAAATGCGGTAACTAAGAGATTAGGATGGGGGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGtt                                 <  2:442158/151‑1 (MQ=255)
                                                                                                                        aaggggttAACTTAGAGATTAGGATTGCGGAAAATAAAAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTg                              <  2:1490813/144‑1 (MQ=255)
                                                                                                                          tGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGt                            >  2:438341/1‑151 (MQ=255)
                                                                                                                           gCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTc                           >  2:851673/1‑151 (MQ=255)
                                                                                                                           gCGATAACTTGGAAATTAGGGGGACGAAAAAAAACACCCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTc                           <  1:478298/151‑1 (MQ=255)
                                                                                                                            cGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCTTCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCg                          >  2:64219/1‑151 (MQ=255)
                                                                                                                            cGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCg                          >  2:1491074/1‑151 (MQ=255)
                                                                                                                            cGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCg                          >  2:119348/1‑151 (MQ=255)
                                                                                                                                   ttAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCAt                   >  1:125431/1‑151 (MQ=255)
                                                                                                                                    tgagagATAGGGATTGGGGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCa                    <  1:98176/148‑1 (MQ=255)
                                                                                                                                           ttAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGgcagc             >  2:1293592/1‑150 (MQ=255)
                                                                                                                                             aGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGcac           >  2:378669/1‑150 (MQ=255)
                                                                                                                                             aGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGcac           >  2:1551276/1‑150 (MQ=255)
                                                                                                                                               gATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAgg        >  1:1203974/1‑151 (MQ=255)
                                                                                                                                                   gCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGc    >  1:889082/1‑151 (MQ=255)
                                                                                                                                                   gCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGc    >  1:1028296/1‑151 (MQ=255)
                                                                                                                                                    cGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCg   >  2:227125/1‑151 (MQ=255)
                                                                                                                                                     ggAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCgg  >  2:325663/1‑151 (MQ=255)
                                                                                                                                                     ggAAAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCg   <  1:1003125/150‑1 (MQ=255)
                                                                                                                                                     ggAAAAAAAAAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCgg  <  2:704142/151‑1 (MQ=255)
                                                                                                                                                         |                                                                                                                                                   
ATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGG  >  NC_000913/5010‑5306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: