Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,356,201	0	T	G	47.1%	93.4 / 18.8	69	T304P (ACC→CCC)	sapA	antimicrobial peptide transport ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (7/29);  new base G (32/0);  total (40/29)
Fisher's exact test for biased strand distribution p-value = 7.02e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGC‑AAAATGGATAGCTGGCTGGCAGCAGG‑CCA‑GGCCAGAACGTCG‑CATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGC  >  NC_000913/1356054‑1356350                                                                                                                                                      |                                                                                                                                                           cGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑Ggtg                                                                                                                                                           <  1:686878/149‑1 (MQ=255)        aTAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGG‑GGGGGTAAAttgc                                                                                                                                                  >  1:1302942/1‑148 (MQ=255)               cACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTTGG‑GGGGTTAAATGCCAGAAAgg                                                                                                                                           >  1:453004/1‑151 (MQ=255)                aCGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGGGGTTAATGCCaaaaa                                                                                                                                             >  2:1006161/1‑144 (MQ=255)                    ggagcgcgATAAAATAGAGGCCGCCTATTCAGCCGTCCCATAATAGATGGATTGCACCAGGCGCTGGTTATTACTCGCCAGCGCTAGCGCATGGCGGACAGCGGGATTAGTTAGCGACCG‑TTTGGC‑GGTATTAAATCCCAGATAGGCGatt                                                                                                                                      <  1:992704/148‑3 (MQ=255)                     gcgcgcGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGACG‑t                                                                                                                                    <  1:300670/151‑1 (MQ=255)                        cgcgGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGACG‑TTCa                                                                                                                                 <  1:323309/151‑1 (MQ=255)                        cgcgGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑GTTTGC‑GGTGTTTAATGCCAAACAGGCGACG‑tt                                                                                                                                   >  1:558408/1‑149 (MQ=255)                         gcgGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTTGC‑GGGGGTAAATGCCAAATaaggggcagtcat                                                                                                                                 >  1:1265946/1‑139 (MQ=255)                         gcgGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGG‑GGTGGTTAATTGCAGagaagggaacgt                                                                                                                                    >  1:728219/1‑137 (MQ=255)                              aaaaTAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGTCGACG‑TTCATCCCa                                                                                                                            <  1:1418848/150‑1 (MQ=255)                              aaaaTAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCa                                                                                                                            <  1:993809/150‑1 (MQ=255)                              aaaaTAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCa                                                                                                                            <  1:605058/150‑1 (MQ=255)                              aaaaTAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCa                                                                                                                            <  2:499655/150‑1 (MQ=255)                                aaTAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTTGC‑GGGGTTTAAAGGCAGATATGCGACG‑TTCCTCCCCgg                                                                                                                          >  2:1188708/1‑150 (MQ=255)                                   agagGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACg                                                                                                                       <  2:386961/150‑1 (MQ=255)                                    gagGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCa                                                                                                                     <  1:262485/151‑1 (MQ=255)                                         cgccgTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGG‑GGCGTTCAATTGCCGACATGCGGCG‑CTCATCCCCgggacccgcggt                                                                                                                >  1:868856/1‑141 (MQ=255)                                          gccgTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTa                                                                                                               <  2:500492/151‑1 (MQ=255)                                            cgTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTTGG‑GGGGGTTAATGTCAAATAGGCGACG‑TTTCTCCCCCGGCGCCGCGTTaa                                                                                                              >  1:646473/1‑150 (MQ=255)                                              tttCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGG‑GGGGTTAAAAGCCAAATAGGCGACG‑TTTATTCCAAGACGCCGCGGTaaaa                                                                                                            >  1:1409697/1‑149 (MQ=255)                                               ttCAGCCGTACCATAATAGATGGATTGCATCAGGCGTTGGTTATTAAGCGCCAGTGCTAGCGCATGGCGGACAGTGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGTATCGTTAAACg                                                                                                           <  1:1193300/150‑1 (MQ=255)                                               ttCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACg                                                                                                           <  2:1162651/150‑1 (MQ=255)                                               ttCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGGGGTTAATGGCCGATAAGGGAAG‑TTCATCCCCGGAAGCAGCgggaaa                                                                                                             >  1:1117529/1‑143 (MQ=255)                                                 cAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTTGC‑GGGGTTTAATGCCAGATAAGGGACG‑TTCATCCCAGGACGCAGGGGTAAAAGc                                                                                                          >  1:1339367/1‑149 (MQ=255)                                                 cAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCaa                                                                                                        <  1:352154/151‑1 (MQ=255)                                                    ccGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTTGG‑GGGGTTAAAAGCCAGAAAGGCGACG‑TTCTTCCCCGGGCGCAGCGTTaaaagga                                                                                                         >  2:330636/1‑143 (MQ=255)                                                     cGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGGGTTTAATACCAGATAGGCGACG‑TTCATCCCCGGACGCAGCGGTAAAAGCaa                                                                                                        >  1:1278608/1‑147 (MQ=255)                                                      gTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAGcgcg                                                                                                   <  1:1544522/151‑1 (MQ=255)                                                       tACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGCGGGGGTTAAAT‑CCAGATAGGCGACG‑TTCATCCCCGGACGCAGCGTTAAAAGCAAACGCgg                                                                                                  >  2:1512359/1‑151 (MQ=255)                                                           ataataGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTTGC‑GGGGGTTAATGCCCGATAAGCGGCGTTTTTTCCCCGGA‑GCAGCGGTAAAAACAAACGCgggg                                                                                                >  1:198794/1‑148 (MQ=255)                                                           ataataGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGgtcg                                                                                              <  1:319944/151‑1 (MQ=255)                                                             aataGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTTGG‑GGGGTTAAATGCCAGAGAGGCGACG‑TTCATTCCCGGACGCAGCGTTTAACGACAACGCGGggcg                                                                                              >  2:1026383/1‑149 (MQ=255)                                                             aataGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGgtcgtc                                                                                            <  1:1219209/151‑1 (MQ=255)                                                                    ggATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGGGGTAAAAGCCAGATAAGGGACG‑GTTATCCCAGGGCGCAGCGGTAAACGCAAACGCCGGGCGTCCAGC‑aa                                                                                     >  2:969377/1‑150 (MQ=255)                                                                            tCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGGGTTAAATAACAAAAAAGGGGCG‑GTCATCCCAGGGCACAGCGTTAAACGCAAGCGCCGGGCGTCCCGC‑aaaaatg                                                                                >  2:673135/1‑144 (MQ=255)                                                                            tCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGGGGTTAAAGCCAGATAAGGGGCG‑TTCATCCCCCGACGCAGCGGTAAAAGCAAACGCGGGGCGGCCCGC‑caaaaagg                                                                               >  1:1426434/1‑144 (MQ=255)                                                                              aGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGGGGTAAAAGCCAGATAGGCGACG‑GTCATCCCCGGACGCAGCGGTAAACGCAAGCGCGGGGCGGCACGC‑caaa                                                                                   >  2:91379/1‑142 (MQ=255)                                                                               gggctgggTTATAAACCG‑CAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGC‑AAAATGGAt                                                                              <  2:149992/139‑1 (MQ=255)                                                                               ggCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGC‑AAAATGGATAgctg                                                                         <  1:589134/151‑1 (MQ=255)                                                                                 cgcTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTTGG‑GGGGTTTAAAGCCAGATAGGCGGCG‑TTCATCCCCGGGCGCCGCGGTAAAAACAAGCGCGGGGCGTCACCC‑CAAAAGGATAgc                                                                           >  1:306978/1‑147 (MQ=255)                                                                                   cTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTTGGG‑GGGGTTAAA‑GCCCGAAAAGGGGCG‑GTCATCCCCGGACGCCGCGGTTAAAGCCAACGCGGGGGGGCACGC‑CAAAAGGGTaaccgg                                                                        >  1:1366719/1‑143 (MQ=255)                                                                                    tGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTTGC‑GGGGTTAAATGCCAGATAGGCGACG‑TTCATCCCCGGACGCAGCGGTAAACGCAAACGCGGGGCGTCACGC‑AAAATGGATAGctggctgg                                                                    >  2:877535/1‑151 (MQ=255)                                                                                      gTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGC‑AAAATGGATAGCTGGCTGgca                                                                  <  2:594409/151‑1 (MQ=255)                                                                                        tattaATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGC‑AAAATGGATAGCTGGCTGgcag                                                                 <  1:122239/150‑1 (MQ=255)                                                                                          ttaATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGGTTAATGCCAGATAAGCGACG‑TTCATCCCAGGACGCCGCGTTAAACGCAAGCGCGGGGCGTCACGC‑CAAATGGATAGctggctgg                                                                    >  2:243265/1‑145 (MQ=255)                                                                                           taATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGC‑AAAATGGATAGCTGGCTGGcagcag                                                              <  2:350304/150‑1 (MQ=255)                                                                                           taATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGGGTTAAAAGCCAGATAAGCGACG‑TTCATCCCCCGACGCAGCGGTAAAAGCAAACGCGGGGCGTCACGC‑AAAATGGATAGCTGGCTGGCAGcagg                                                             >  2:481100/1‑151 (MQ=255)                                                                                                gCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGG‑GGGGTTAAAAGCCAGATAGGCGACG‑GTCATCCCCGGAAGCAGCGGTTAAAGCAAGCGCGGGGCGGCACGC‑AAAAAGGATAGCTGGGTGGGAGcagg‑cca‑gg                                                      >  2:162933/1‑151 (MQ=255)                                                                                                gCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGGGTTTAATGCCAGATAAGGGGCG‑TTCATCCCAGGACGCAACGCTAAAAGCAAGCGCGGGGCGTCACGC‑CAAAAGGAGAGCTGGCTGGCAGcagggccgg                                                        >  2:938794/1‑146 (MQ=255)                                                                                                  cAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTTGG‑GGGGGTAAAAGCCCGATAAGGGACG‑TTCATCCCCGGGCGCAGCGTTAAAAGCAAAAGCGGGGCGGCACGC‑AAAATGGGGAGGCGGGTGGGAgcagg‑gca‑gggc                                                    >  1:564888/1‑149 (MQ=255)                                                                                                   aGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGG‑GGGGTTAAAAGCCCGATAGGCGACG‑GTCATCCCCGGGCGCAGCGTTAAAAGCAAGCGCGGGGCGTCACGC‑AAAAAGGATAGCTGGCTGGCAGCagg‑cca‑ggcc                                                    >  2:1540688/1‑150 (MQ=255)                                                                                                       ccAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGC‑AAAATGGATAGCTGGCTGGCAGCAGG‑CCA‑GGCCAGAAc                                               <  1:1030348/151‑1 (MQ=255)                                                                                                           cgcATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGC‑AAAATGGATAGCTGGCTGGCAGCAGG‑CCA‑GGCCAGAACGTCg                                           <  2:453068/151‑1 (MQ=255)                                                                                                              aTGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTTGC‑GGGGGTAAAATCCAGAAAGGGGGCG‑GTTATCCCCGGACGCAGCGGTAAACGCAAACGCGGGGCGGCCCCC‑AAAAAGGGAAGCTTGGCGGCCGgagg‑cccagcccggaagcc                                             >  1:767834/1‑134 (MQ=255)                                                                                                                ggCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGC‑AAAATGGATAGCTGGCTGGCAGCAGG‑CCA‑GGCCAGAACGTCG‑CAtt                                      <  2:349978/150‑1 (MQ=255)                                                                                                                 gCGGACAGCGGGATTATTTAGCGGCGG‑TTTTGG‑GGGGGTAAAAGCCCGATAGGCGGCG‑GTCCTCCCAGGACGCCGCGGTAAACGCAAGCGCGGGGCGGCACGC‑AAAAAGGATAGCTGGCTGGCAGCAGGCCCA‑GGCC‑GAAAGGCG‑CATTcc                                    >  2:1394538/1‑151 (MQ=255)                                                                                                                      cAGCGGGATTATTTAGCGGCGG‑GTTTGC‑GGGGGTTAATGGCCGATAGGGGAAG‑TTCATCCCAAGGCGCCGCGGTAAACGCAAACGCGGGGCGGCCCGC‑AAAAAGGGTAGCTGGGTGGGCGGAGG‑CCAGGGCCGGAACG‑CG‑CATTtccc                                  >  1:490650/1‑148 (MQ=255)                                                                                                                        gCGGGATTATTTAGCGGCGG‑TTTTGC‑GGGGTTAAATGCCAGATAGGGGACG‑TTTATCCCCGGACGCAGCGGTAAAAGCAAAAGCGGGGGGTTACGC‑AAAAAAGATAGATGGCTGGCAGCAGG‑CCA‑GGCCCGAACGGCG‑CCTTcccc                                  >  1:557253/1‑146 (MQ=255)                                                                                                                        gCGGGATTATTTAGCGGCGG‑TTTGGC‑GGGGTTTAATGCCAGATAAGGGACG‑GTTATCCCCGGACGCAGCGGTAAACGCAAGCGCGGGGCGGCACGC‑AAAAAGGGTAGCTGGCTGGGAGCAGG‑CCA‑GGCCCGAAAGGCC‑CATTcccc                                  >  2:1539219/1‑146 (MQ=255)                                                                                                                          gggATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGC‑AAAATGGATAGCTGGCTGGCAGCAGG‑CCA‑GGCCAGAACGTCG‑CATTCCCCGGTCAgg                           <  2:307027/151‑1 (MQ=255)                                                                                                                          gggATTATTTAGCGGCGG‑TTTGGC‑GGGGGTAAATGCCCAATAAGGGGCG‑TTTATCCCCGGGCGCAGCGGTTAACGCAAACGCGGGGCGTCACGCAAAAATGGA‑AGCGGGGTGGCAGCAGG‑GCA‑GGCCAGAAAGGCGCCTTTCCCCggccgg                            >  1:216651/1‑147 (MQ=255)                                                                                                                                attTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGC‑AAAATGGATAGCTGGCTGGCAGCAGG‑CCA‑GGCCAGAACGTCG‑CATTCCCCGGTCAGGAGttt                      <  1:425972/150‑1 (MQ=255)                                                                                                                                attTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGC‑AAAATGGATAGCTGGCTGGCAGCAGG‑CCA‑GGCCAGAACGTCG‑CATTCCCCGGTCAGGAGTTTc                     <  2:1202437/151‑1 (MQ=255)                                                                                                                                      cggcgg‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGC‑AAAATGGATAGCTGGCTGGCAGCAGG‑CCA‑GGCCAGAACGTCG‑CATTCCCCGGTCAGGAGTTTCGACAGa               <  2:1266068/151‑1 (MQ=255)                                                                                                                                           g‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGC‑AAAATGGATAGCTGGCTGGCAGCAGG‑CCA‑GGCCAGAACGTCG‑CATTCCCCGGTCAGGAGTTTCGACAGACGTcc          >  2:886324/1‑151 (MQ=255)                                                                                                                                           g‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGC‑AAAATGGATAGCTGGCTGGCAGCAGG‑CCA‑GGCCAGAACGTCG‑CATTCCCCGGTCAGGAGTTTCGACAGACGTcc          <  1:968600/151‑1 (MQ=255)                                                                                                                                             tttGGC‑GGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGC‑AAAATGGATAGCTGGCTGGCAGCAGG‑CCA‑GGCCAGAACGTCG‑CATTCCCCGGTCAGGAGTTTCGACAGACGTcc          >  1:968442/1‑150 (MQ=255)                                                                                                                                              ttGGC‑GGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGC‑AAAATGGATAGCTGGCTGGCAGCAGG‑CCG‑GGCCAGAACGTCG‑CATTCCCCGGTCAGGAGTTTCGACAGACGTGCgg        >  2:676981/1‑151 (MQ=255)                                                                                                                                              ttGGC‑GGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGC‑AAAATGGATAGCTGGCTGGCAGCAGG‑CCA‑GGCCAGAACGTCG‑CATTCCCCGGTCAGGAGTTTCGACAGACGTCCgg        >  1:1132483/1‑151 (MQ=255)                                                                                                                                                     gtgtTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGC‑AAAATGGATAGCTGGCTGGCAGCAGG‑CCA‑GGCCAGAACGTCG‑CATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTgccgc  <  2:1563705/151‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                           CGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGC‑GGTGTTAAATGCCAGATAGGCGACG‑TTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGC‑AAAATGGATAGCTGGCTGGCAGCAGG‑CCA‑GGCCAGAACGTCG‑CATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGC  >  NC_000913/1356054‑1356350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑