Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091329,4860CA41.7% 167.8 / 53.8 96intergenic (+291/‑165)dapB/carAdihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base C (41/15);  new base A (0/40);  total (41/55)
Fisher's exact test for biased strand distribution p-value = 7.11e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

TTTTGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATA  >  NC_000913/29337‑29634
                                                                                                                                                      |                                                                                                                                                    
ttttGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCcac                                                                                                                                                      >  1:1496073/1‑150 (MQ=255)
ttttGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCcac                                                                                                                                                      >  1:673341/1‑150 (MQ=255)
 tttGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCcaca                                                                                                                                                     <  1:669667/150‑1 (MQ=255)
    gttgttTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAaat                                                                                                                                                 <  1:759762/151‑1 (MQ=255)
    gttgttTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAaat                                                                                                                                                 <  2:333651/151‑1 (MQ=255)
      tgttTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAaata                                                                                                                                                <  2:47558/150‑1 (MQ=255)
       gttTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAaataat                                                                                                                                              >  2:635865/1‑151 (MQ=255)
       gttTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAaataat                                                                                                                                              <  1:1230685/151‑1 (MQ=255)
       gttTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAaataa                                                                                                                                               <  2:392056/150‑1 (MQ=255)
         tttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAataata                                                                                                                                             <  1:1519150/150‑1 (MQ=255)
         tttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAataata                                                                                                                                             <  1:1560030/150‑1 (MQ=255)
         tttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAataata                                                                                                                                             <  1:583091/150‑1 (MQ=255)
         tttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAatat                                                                                                                                            >  2:411379/1‑151 (MQ=255)
           cgaTGTAAATTTTGACCATTTGGTCCGCTTTATTCTGCTCGTTTTTATTTAATACAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGCCGCTTAATGCCTGTAAAACATGCA‑TGAGCCCCAAAATAAtata                                                                                                                                           >  1:205068/3‑150 (MQ=255)
               gTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATaaaaa                                                                                                                                       <  2:494815/150‑1 (MQ=255)
                 aaaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAAt                                                                                                                                     <  1:451872/150‑1 (MQ=255)
                 aaaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATc                                                                                                                                    <  2:1377994/151‑1 (MQ=255)
                  aaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATcc                                                                                                                                   <  1:862236/151‑1 (MQ=255)
                   aTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATccc                                                                                                                                  >  2:1501051/1‑151 (MQ=255)
                        gACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCa                                                                                                                              <  2:957550/150‑1 (MQ=255)
                        gACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCa                                                                                                                              <  2:764727/150‑1 (MQ=255)
                        gACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCAt                                                                                                                             >  1:672376/1‑151 (MQ=255)
                        gACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTAATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCAt                                                                                                                             >  1:1239636/1‑151 (MQ=255)
                          ccATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCAtt                                                                                                                            >  2:296671/1‑150 (MQ=255)
                              ttGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGtt                                                                                                                       >  2:935174/1‑151 (MQ=255)
                                 gTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGa                                                                                                                     >  2:1385316/1‑150 (MQ=255)
                                        tttttCTCCTCTTTTTTATTTCATAAAATCTTCTTGCTGCGAAAGCTTTTCACAAAAGGGTTTAGATGATTTTTTTTTCGTTTAAGGTCTTAAAAACATGAA‑GGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAg                                                                                                              <  1:1079855/150‑1 (MQ=255)
                                          tttCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAgcgc                                                                                                           >  2:892240/1‑151 (MQ=255)
                                               gCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCat                                                                                                       >  1:1031906/1‑150 (MQ=255)
                                                    tttttATTTCATGCATTCTTCTTGCTGCGAACGCTTTTTCCAGAACAGTTTAAATAATTTTTTTGTCGTTAAATCCCTAAAAAACATGCA‑TGCGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATAtctc                                                                                                  <  1:1139136/150‑1 (MQ=255)
                                                     ttttATTTCAAGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAACACATGAA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATAtctc                                                                                                  >  1:359112/1‑149 (MQ=255)
                                                        tttttCATGCATTCTTTTTGCTGCGAAAGCGTTTTCCAGAAGGGGTTAGATGTTTTTTTGGTCGTTAAAGGCTTGAAAAACAGGCA‑TGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGa                                                                                              <  1:1318244/148‑1 (MQ=255)
                                                         aTTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAAt                                                                                            >  1:1337410/1‑151 (MQ=255)
                                                         aTTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAAt                                                                                            >  2:1427660/1‑151 (MQ=255)
                                                           ttCATGCAATCTTCTTGCTGCGCAAGCTTTTCCCAAAACAGGTTAGATGATTTTTTTGTCGTTTAATGCTTGTAAAACATGCA‑TGACCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATg                                                                                           <  2:776217/150‑1 (MQ=255)
                                                           ttCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgc                                                                                          >  2:1199185/1‑151 (MQ=255)
                                                           ttCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgc                                                                                          >  2:1561869/1‑151 (MQ=255)
                                                           ttCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgc                                                                                          >  1:1493989/1‑151 (MQ=255)
                                                            tCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCTTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgcc                                                                                         >  2:1544834/1‑151 (MQ=255)
                                                                gCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGccgccg                                                                                     >  2:261038/1‑151 (MQ=255)
                                                                 cAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGt                                                                                    >  2:924706/1‑151 (MQ=255)
                                                                  attcttctTGCTGCGCAAGCTTTTTCCAGAACAGTTTAGATGATTTTTTTGTCGCTTAATGCTTAAAAAACATGCA‑TGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccgcc                                                                                      <  2:879426/146‑1 (MQ=255)
                                                                    tcttctTCCTGCGCAAGCTTTTCCCAAAACAGGTAAAATTATTTTTTTTTCGTTTAATCCCTGAAAAACATGCA‑TGACCCACAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGttt                                                                                  <  1:1500905/150‑1 (MQ=255)
                                                                       tcttGCTGCGAAAGCTTTTTCCAGAACAGGTTAGATGATTTTTTTGTCGTTTAATGCTTGAAAAACATGCA‑TGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGttt                                                                                  <  2:873427/147‑1 (MQ=255)
                                                                         ttGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGcc                                                                               >  2:1143731/1‑148 (MQ=255)
                                                                             tgcGCAAGCTTTTTCCAAAACAGGTTAAATGATTTTTTTGTCGCTTAATGCTTTAAAAACATGCA‑TGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGa                                                                            <  2:649228/147‑1 (MQ=255)
                                                                              gcgcACGCTTTTTCCAGAACAGTTTAGATTATTTTTTTTTCCTTTAATCCCTTAAAAACATGCA‑TGACCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAAtt                                                                        <  1:1029864/150‑1 (MQ=255)
                                                                               cgcAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtc                                                                       >  1:915448/1‑150 (MQ=255)
                                                                                  aggttttttCCAGAACAGGTTAGATGATTTTTTTGTCGTTTAATGCTTGAAAAACATGCA‑TGAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgtc                                                                    <  2:66868/145‑1 (MQ=255)
                                                                                       ttttCAAAAACAGTTTAGATGTTTTTTTTGTCGTTTAATCCCTAAAAAACATGCA‑TGACCAAAAAAATAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGaaa                                                                          <  1:1099301/139‑1 (MQ=255)
                                                                                       tttaCCAGAAAAGGTTAAATTATTTTTTGATCATTTAATGCCTGAAAAACATGTA‑CGAAAAAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgtc                                                                    <  2:534950/145‑1 (MQ=255)
                                                                                            cAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGc                                                             >  2:105076/1‑147 (MQ=255)
                                                                                             aaaaCAGTTTAGATGTTTTTTTTGCCTTTTAATGCCTAAAAAACATGCT‑GGACCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGAtt                                                        <  1:1188215/149‑1 (MQ=255)
                                                                                               aaCGGGTTAGATTTTTTTTTTGTCGTTAAATGCTGGAAAAACATGCA‑TGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGAttt                                                       <  2:1226953/150‑1 (MQ=255)
                                                                                               aaCAGGTTAGATGATTTTTTTGTCGCTAAATGCCTGAAAAACATGCA‑TGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGAttt                                                       <  2:239552/150‑1 (MQ=255)
                                                                                               aaCAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTg                                                      >  2:610175/1‑151 (MQ=255)
                                                                                                   ggTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAtt                                                  >  2:38893/1‑151 (MQ=255)
                                                                                                     tgaGATGATTTTTTTTGCCCTTAATGCCTAAAAAACTTGAA‑TGACCCAAAAAATAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAtt                                                  <  2:1432007/147‑1 (MQ=255)
                                                                                                     taaGATGTTTTTTTTGTCGCTTAATGCTTAAAAAACATCCA‑TGACCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTg                                                 <  2:632446/148‑1 (MQ=255)
                                                                                                       agatttttttttttTCTTTTAATGCCTAAAAAACATGCA‑TGACCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGa                                                <  1:231701/141‑1 (MQ=255)
                                                                                                       aGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAtt                                              >  2:1220838/1‑151 (MQ=255)
                                                                                                       aGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAtt                                              >  2:271268/1‑151 (MQ=255)
                                                                                                             tttttttGTCGTTAAATGCCTAAAAAACATGAA‑TGAGCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAtt                                              <  2:31466/143‑1 (MQ=255)
                                                                                                               tttttGTCGTTTAATGCTTAAAAAACATGCA‑TGACCCAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtc                                        <  2:809285/149‑1 (MQ=255)
                                                                                                               tttttGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcat                                      >  2:1287093/1‑151 (MQ=255)
                                                                                                               tttttGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcat                                      >  2:210055/1‑151 (MQ=255)
                                                                                                               tttttGTCGCTAAATGCCTGAAAAACATGTG‑GAAGCCAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtc                                        <  2:532575/149‑1 (MQ=255)
                                                                                                                ttttGTCGCTTAATGCCTTAAAAACATCAA‑TGACCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcat                                      <  1:1183053/150‑1 (MQ=255)
                                                                                                                 tttGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcatc                                     >  1:1018274/1‑150 (MQ=255)
                                                                                                                   ttttctttAATTCCCGGAAAAAAAGCATTGCGCCAAAAAATAATATAAAAACTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcatca                                    <  1:1491819/144‑1 (MQ=255)
                                                                                                                   tGTCGTTTAATGCTTGAAAAACATGCA‑TGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTcatcat                                   <  2:1496219/150‑1 (MQ=255)
                                                                                                                   tGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTcatcat                                   >  1:489921/1‑150 (MQ=255)
                                                                                                                     tCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgt                                >  2:1054954/1‑151 (MQ=255)
                                                                                                                      cGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgtg                               >  2:472129/1‑151 (MQ=255)
                                                                                                                       gCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgtg                               >  1:221481/1‑150 (MQ=255)
                                                                                                                         ttaagccgggaaaaaCATGTA‑TGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgt                                <  2:1536520/136‑1 (MQ=255)
                                                                                                                         ttAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAt                            >  2:1291566/1‑151 (MQ=255)
                                                                                                                         ttAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAt                            >  1:1061586/1‑151 (MQ=255)
                                                                                                                           aaTGCCTGAAAAACATGCA‑TGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAtt                           <  2:185531/150‑1 (MQ=255)
                                                                                                                           aaTGCCTAAAAACCATGCA‑TGACCAAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAtt                           <  1:935072/150‑1 (MQ=255)
                                                                                                                           aaTCCCTGAAAAACATGAA‑GGACCAAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAtt                           <  2:845327/150‑1 (MQ=255)
                                                                                                                            aTGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTa                          >  2:906450/1‑150 (MQ=255)
                                                                                                                              gCCTGAAAAACATGCA‑TGAGCCAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAat                        <  2:187047/150‑1 (MQ=255)
                                                                                                                               cctaaaaaaCATGCA‑TGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAat                        <  1:1517380/144‑1 (MQ=255)
                                                                                                                                 tgaaaaaCATGCA‑TGGCCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAatat                      <  2:1520576/146‑1 (MQ=255)
                                                                                                                                  gaaaaaCATGCA‑TGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATg                     <  2:231083/147‑1 (MQ=255)
                                                                                                                                    aaaaCATGCA‑TGACCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCa                   <  1:1544684/149‑1 (MQ=255)
                                                                                                                                     aaaacttgatttgtccAAAAAATAATAAAAAAATTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAatat                      <  1:1113689/132‑1 (MQ=255)
                                                                                                                                      aaCATGCA‑TGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATa               <  1:427574/151‑1 (MQ=255)
                                                                                                                                      aaCATGAA‑TGACCCAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATa               <  2:886631/151‑1 (MQ=255)
                                                                                                                                        catgca‑tgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGAGAATTAATATGCAAATaa              >  1:751546/1‑150 (MQ=255)
                                                                                                                                         atgca‑tgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaa              >  1:1547636/1‑149 (MQ=255)
                                                                                                                                          tgaa‑tgACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaaa             <  2:1380203/146‑1 (MQ=255)
                                                                                                                                           tgaaggcgCCAAAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaa              <  2:613650/141‑1 (MQ=255)
                                                                                                                                            ca‑tgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg          >  1:1540338/1‑150 (MQ=255)
                                                                                                                                             a‑tgACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagt           <  1:749381/148‑1 (MQ=255)
                                                                                                                                               tgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagt       >  2:787827/1‑151 (MQ=255)
                                                                                                                                                gacccAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAag            <  2:1301869/142‑1 (MQ=255)
                                                                                                                                                    cacaAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAat   >  1:124951/1‑150 (MQ=255)
                                                                                                                                                     acaAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAata  <  1:565293/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
TTTTGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATA  >  NC_000913/29337‑29634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑