Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091340,5150GT41.9% 111.0 / 43.5 86Q473K (CAA→AAA) caiTputative transporter
Reads supporting (aligned to +/- strand):  ref base G (11/39);  new base T (36/0);  total (47/39)
Fisher's exact test for biased strand distribution p-value = 8.43e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCATTTAAATTAAAATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCG‑AT‑AATG‑CCAACCAGAATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCG‑CGGCAAGTGGACATCGCCAGGGTATAAGAGCAGGCGTT  >  NC_000913/40366‑40662
                                                                                                                                                       |                                                                                                                                                          
atcatTTAAATTAAAATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGa                                                                                                                                                           <  2:358093/151‑1 (MQ=255)
   atTTAAATTAAAATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑T‑c                                                                                                                                                      <  2:856357/151‑1 (MQ=255)
       aaattaaaATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCAGAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGtt                                                                                                                                                 <  1:251314/151‑1 (MQ=255)
        aattaaaATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTTGA‑T‑C‑GGgtt                                                                                                                                                >  1:728197/1‑151 (MQ=255)
        aattaaaATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGttt                                                                                                                                                <  1:133944/151‑1 (MQ=255)
         attaaaATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTTAA‑T‑C‑GGgtt                                                                                                                                                >  2:1312932/1‑150 (MQ=255)
         attaaaATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGTTTc                                                                                                                                               <  2:32139/151‑1 (MQ=255)
           taaaATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTTAA‑T‑C‑GGttt                                                                                                                                                >  2:1020663/1‑148 (MQ=255)
             aaaTCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTTAA‑A‑C‑GGgttttg                                                                                                                                             >  2:316144/1‑146 (MQ=255)
             aaaTCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGTTTCAGgc                                                                                                                                           <  1:285823/151‑1 (MQ=255)
                 ccATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGTTTCAGgccgcc                                                                                                                                       <  1:1494783/151‑1 (MQ=255)
                   aTCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTTTA‑T‑C‑TGGTTCTGgccgcc                                                                                                                                       >  1:733089/1‑149 (MQ=255)
                   aTCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTTAA‑T‑T‑GGGTTTAGgccgcc                                                                                                                                       >  2:1088668/1‑149 (MQ=255)
                      tttCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGG‑TTTGAA‑T‑‑‑TGTTTCAGGgccccgcg                                                                                                                                    >  1:1239473/1‑147 (MQ=255)
                       ttCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTTGA‑T‑T‑GGGTTCCCGCCGCCGAgc                                                                                                                                   >  2:906313/1‑149 (MQ=255)
                       ttCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTTAA‑A‑A‑GGGTTTAGGCCCCCCGgcgc                                                                                                                                 >  2:1235348/1‑151 (MQ=255)
                       ttCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGTTTCAGGCCGCCGa                                                                                                                                     <  1:1481012/147‑1 (MQ=255)
                       ttCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGTTTCAGGCCGCCGAg                                                                                                                                    <  2:392046/148‑1 (MQ=255)
                       ttCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGTTTCAGGCCGCCGAg                                                                                                                                    <  2:757706/148‑1 (MQ=255)
                       ttCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGTTTCAGGCCGCCGAg                                                                                                                                    <  2:1029113/148‑1 (MQ=255)
                        tCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGTTTCAGGCCGCCGAgcgc                                                                                                                                 <  2:490107/150‑1 (MQ=255)
                          aaCCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTTAA‑T‑C‑GGGTTTAGGccgccgcg                                                                                                                                    >  1:1168316/1‑143 (MQ=255)
                          aaCCTCTTGATATTTTGGGGGTTAATTAATCGTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGTTTCAGGCCGCCGAGCGCcag                                                                                                                              <  2:218796/151‑1 (MQ=255)
                           aCCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTTAA‑T‑C‑GGGTTTAGGCCGCCGAGCGcc                                                                                                                                >  2:942755/1‑148 (MQ=255)
                               ctTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGTTTCAGGCCGCCGAGCGCcagcag                                                                                                                           <  2:1430820/149‑1 (MQ=255)
                                 tGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTTCA‑T‑C‑GGTTTCAGGCCGCCGAGCGCCCGCCGaa                                                                                                                         >  2:238854/1‑149 (MQ=255)
                                 tGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑ATTGTA‑T‑C‑GGGTTTAGGCACCCCCGCGCCGGCAGaa                                                                                                                         >  1:680679/1‑149 (MQ=255)
                                  gATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTTTA‑T‑T‑GGGTTTTGGCCGCCGCGCGCccgcaggaa                                                                                                                        >  1:1017309/1‑146 (MQ=255)
                                  gATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTTAA‑T‑C‑GGTTTTAGGCCGCCCAGCGCCAGCAGaa                                                                                                                         >  1:875400/1‑148 (MQ=255)
                                  gATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGTTTCAGGCCGCCGAGCGCCAGCAGAAc                                                                                                                        <  1:1035338/149‑1 (MQ=255)
                                   atatTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTTTT‑T‑C‑GGGTTCAGCGCGCCGACCGCcag                                                                                                                              >  1:266653/1‑142 (MQ=255)
                                     atTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTTAA‑T‑C‑GGTTTTAGGCCGCCGAGCGCCCGCAGAaaaaaa                                                                                                                    >  2:977526/1‑148 (MQ=255)
                                     atTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTTAA‑T‑C‑GGTTTTAGGCCGCCCGGCGCCCGCAGAaaaa                                                                                                                      >  2:480179/1‑148 (MQ=255)
                                     atTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTTAA‑T‑C‑GGGTTTAGGCCGCCCGGCGCCAGGAGAaaaaaa                                                                                                                    >  2:623837/1‑148 (MQ=255)
                                     atTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGTTTCAGGCCGCCGAGCGCCAGCAGAACAATa                                                                                                                    <  2:343310/150‑1 (MQ=255)
                                      ttttGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTTAA‑T‑C‑GGGTTTAAGCCGCCCGGCGGccgcaggaaaaaa                                                                                                                    >  1:756543/1‑142 (MQ=255)
                                      ttttGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGTTTCAGGCCGCCGAGCGCCAGCAGAACAATAc                                                                                                                   <  1:1320546/150‑1 (MQ=255)
                                         tGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GGTTTTGAA‑T‑C‑GGTTTCGGCAGGCCGGCCGCC‑GCAGAACaaa                                                                                                                     >  1:940070/1‑144 (MQ=255)
                                             ggTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTTAA‑T‑C‑GGGTTTAGGCCGCCGAGGGCCCGCAGAAAAAAAACC‑AT‑aa                                                                                                           >  2:1342673/1‑149 (MQ=255)
                                             ggTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCG‑AT‑AATg                                                                                                         <  1:1088634/151‑1 (MQ=255)
                                                taattaatCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGGTTCCTGCCCCCGCGCGCCAGCAGAACAAAACCG‑AT‑AATA‑cc                                                                                                      >  2:30064/1‑150 (MQ=255)
                                                 aattaatCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GGTTTTGAT‑T‑G‑GGTTCCGGGCCCCCG‑CAGCCAGCAGAACAATaaccgtaa                                                                                                             >  2:392446/1‑138 (MQ=255)
                                                 aattaatCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCG‑AT‑AATG‑CCaa                                                                                                    <  1:1460974/151‑1 (MQ=255)
                                                    taatCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GGTTTTTAA‑T‑T‑GGTTTC‑GGCCGCCCCGCGGCCGGAGGAAAATACCC‑ATAAATG‑CCacc                                                                                                   >  2:418027/1‑148 (MQ=255)
                                                         tttCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTTTA‑T‑A‑GGTTTTAGGGCGCCCCGGGCCAGCCGGACAAAAACC‑AT‑AAAG‑TCCACCAgca                                                                                              >  1:1372620/1‑147 (MQ=255)
                                                         tttCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTTAA‑TAT‑GGTTTC‑GGCCGCCCAGCGCCCCCCGAACAAAAACG‑AT‑AAAT‑CCCACCaaaa                                                                                              >  1:875859/1‑149 (MQ=255)
                                                          ttCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTTAA‑T‑T‑GGTTTCTGGCCGCCAAGCGGCCGCGAAACAAAAACC‑AT‑AAAG‑ACAACCACAAATg                                                                                           >  1:286821/1‑151 (MQ=255)
                                                          ttCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCG‑AT‑AATG‑CCAACCAGAAt                                                                                             <  1:645509/149‑1 (MQ=255)
                                                           tCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTTGA‑T‑A‑GGGTTGAGGGGGCCCCGCGGCGGCAGAAGAAGAACGAAA‑AATG‑CGAAccgaaatg                                                                                            >  1:1010320/1‑144 (MQ=255)
                                                                ttCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTTAA‑T‑C‑GGTTTTAGGCCGCCGGGCGCCAGCAGAAAAAAAACG‑AT‑AAAG‑CCCACCAGAAATGACCaa                                                                                      >  2:856373/1‑150 (MQ=255)
                                                                 tCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑A‑A‑GGGTTTAGGCCGCCGAGCGCCCGCAGAACAAAAACG‑AT‑AATG‑ACAACCAGAATTGAACAAcc                                                                                    >  2:867809/1‑151 (MQ=255)
                                                                   tGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTTAA‑T‑C‑GGGTTTAGGGCCCCCAGCGCCCCCCGAAAAAAAACG‑AA‑AATG‑CCAAACAGAAAAGAcccaac                                                                                    >  1:1048901/1‑145 (MQ=255)
                                                                   tGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑T‑A‑GGGTTTTGGCCGCCCAGCGCCCGCAGAACAAAAACG‑AT‑AAAG‑CCAACCAGAAATGACCaa                                                                                      >  2:549784/1‑147 (MQ=255)
                                                                    gTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTTAA‑A‑C‑GGGTTTAGGGCGCCCGGGGCCAGCAGAAAAAAAACG‑AT‑AATT‑CCAACCAGAAATGaacca                                                                                      >  2:644049/1‑142 (MQ=255)
                                                                    gTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑A‑C‑GGTTTCAGGGCCCCGAGCGCCCGCCGGACAATACCC‑AA‑AATA‑TCAACCAAAAATTACCaaac                                                                                    >  2:1156961/1‑146 (MQ=255)
                                                                      ttCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGCTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑CTTTTA‑G‑C‑GAGGTCCGGCCGCCCGGCGCCCGGAGACGAACAACC‑CT‑AATA‑CCAACCCGACATGAACAAcccca                                                                                 >  1:890773/1‑146 (MQ=255)
                                                                       tCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTTAA‑T‑T‑GGGTTTAGGCCGCCGAGCGCCCGCCGAACAAAAACG‑AT‑AAAT‑CCAACCAAAATTGAACAAcccaaa                                                                                >  2:55123/1‑145 (MQ=255)
                                                                         gcgTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCG‑AT‑AATG‑CCAACCAGAATTGACCAACCGATACGCa                                                                            <  1:1269140/151‑1 (MQ=255)
                                                                           gTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTTAA‑T‑C‑GGGTTCAGGCCGCCGAGCGCCAGCAGAAAAAAACCC‑AT‑AATG‑CCCACCAGAAATGACCAACCCCAACGCAcc                                                                          >  1:214677/1‑151 (MQ=255)
                                                                               ttAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCG‑AT‑AATG‑CCAACCAGAATTGACCAACCGATACGCACca                                                                         <  2:1042261/148‑1 (MQ=255)
                                                                                taataaAGGAGAGCGTCACCATAATGTTGATGAATAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGTTTCAGGCCGCCGATCGCCAGCAGAACAATACCG‑AT‑AATG‑CCAACCAGAATTGACCAACCGATACGCACcagca                                                                      <  2:1223315/150‑1 (MQ=255)
                                                                                  ataaAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTTAATT‑G‑GGTTTCA‑GCCGCCGAGCGCCCGCAGAACAAAAACC‑CT‑AAAT‑GCAACCCGAAATTACCAACCCATACCCAcc                                                                          >  2:711180/1‑144 (MQ=255)
                                                                                     aaGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCG‑AT‑AATG‑CCAACCAGAATTGACCAACCGATACGCACCAGCAGAggtg                                                                <  2:235963/151‑1 (MQ=255)
                                                                                         agagCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCG‑AT‑AATG‑CCAACCAGAATTGACCAACCGATACGCACCAGCAGAGGTGGttc                                                            <  1:649535/151‑1 (MQ=255)
                                                                                           agCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCG‑AT‑AATG‑CCAACCAGAATTGACCAACCGATACGCACCAGCAGAGGTGGttctt                                                          <  1:602051/151‑1 (MQ=255)
                                                                                                 aCCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCG‑AT‑AATG‑CCAACCAGAATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCAt                                                    <  2:175590/151‑1 (MQ=255)
                                                                                                       aTGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCG‑AT‑AATG‑CCAACCAGAATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTa                                              <  1:1113943/151‑1 (MQ=255)
                                                                                                          ttGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTTAA‑T‑T‑GGTTTTAGGCCGCCCAGCGCCCGCAGAAAAAAAACC‑AT‑AATT‑CCAAACAGAATTGACCAACCCATACGCACCAGCAGAGGGGGTTCTTCACCAACCCGTAc                                             >  2:240613/1‑149 (MQ=255)
                                                                                                            gACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGGA‑T‑C‑GGGTTTAAGCCGCCCAGCGCCCGCCGAACAAAACCC‑AA‑AAAG‑CCAACCAGAAATGAACCACCCATAAGCCCCACCAGAAGGGGGTCTTCACCAAcgc                                                 >  1:1405469/1‑143 (MQ=255)
                                                                                                            gACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCG‑AT‑AATG‑CCAACCAGAATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTc                                          <  1:166097/150‑1 (MQ=255)
                                                                                                                   aaCAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTTAA‑T‑T‑GGTTTCAGGCCGCCCAGCGCCCGCAGAAAAAAACCG‑AT‑AATG‑CCCACCAGAAATGACCAACCCATACGCACCCGCAAAGGGGGGTTTTTACCCTCGCGGACTTTG‑Cgg                                     >  2:1151637/1‑147 (MQ=255)
                                                                                                                     cAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCG‑AT‑AATG‑CCAACCAGAATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCG‑CGGCAAGTg                               <  2:1505413/151‑1 (MQ=255)
                                                                                                                       gCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTTTA‑A‑C‑GGGTTTAGGGCCCCCAGCGCCCGCAGAACAATACCC‑AT‑AATGCCCAACCA‑AAATGACCAAACCATAAGCCCCAGCCGAGGGGGGTTTTTACCCACCCGCACTTccccgccaatg                                >  2:664073/1‑139 (MQ=255)
                                                                                                                        cGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCG‑AT‑AATG‑CCAACCAGAATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCG‑CGGCAAGTGGAc                            <  1:736858/151‑1 (MQ=255)
                                                                                                                           gCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCG‑AT‑AATG‑CCAACCAGAATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCG‑CGGCAAGTGGACATc                         <  1:1047500/151‑1 (MQ=255)
                                                                                                                           gCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCG‑AT‑AATG‑CCAACCAGAATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCG‑CGGCAAGTGGACATc                         <  1:61578/151‑1 (MQ=255)
                                                                                                                             aTCCTCCGGCGATAATGGC‑GG‑TTTTGA‑T‑T‑GGTTTTACGCCCCCGAGCGCCAGCCGAAAAAAACCG‑AG‑AATA‑CCCACCAGAAATTACCAAACGAGACGCACCCCCCAAGGGGGGTTTTTACCCACGCCTACTTCGCCGGCAAggg                               >  1:1049404/1‑142 (MQ=255)
                                                                                                                                 tccGGCGATAATGGC‑GG‑TTTTTA‑T‑CGGGTTTCCGGCC‑CCCCGCGCCCCCCGAAAAAAAACG‑ATAAATC‑CCAACC‑GAAATGAACAACCGCCACGCCCCCCCAGAGGGGGGTTTTTACCACCCCGTACTTCG‑CGGGAAgggggaaaaccc                      >  1:1040343/1‑140 (MQ=255)
                                                                                                                                  ccGGCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCG‑AT‑AATG‑CCAACCAGAATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCG‑CGGCAAGTGGACATCGCCAggg                  <  1:77634/151‑1 (MQ=255)
                                                                                                                                  ccGGCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCG‑AT‑AATG‑CCAACCAGAATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCG‑CGGCAAGTGGACATCGCCAg                    <  1:1343250/149‑1 (MQ=255)
                                                                                                                                    ggCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCG‑AT‑AATG‑CCAACCAGAATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCG‑CGGCAAGTGGACATCGCCAGGGta                <  2:1228707/151‑1 (MQ=255)
                                                                                                                                     gCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCG‑AT‑AATG‑CCAACCAGAATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCG‑CGGCAAGTGGACATCGCCAGGGtat               <  1:551615/151‑1 (MQ=255)
                                                                                                                                             ggC‑GG‑TTTGAA‑T‑C‑GGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCG‑AT‑AATG‑CCAACCAGAATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCG‑CGGCAAGTGGACATCGCCAGGGTATAAGAGCa        <  1:688078/150‑1 (MQ=255)
                                                                                                                                               c‑GG‑TTTGAA‑T‑C‑GGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCG‑AT‑AATG‑CCAACCAGAATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCG‑CGGCAAGTGGACATCGCCAGGGTATAAGAGCAGGc     >  2:879449/1‑151 (MQ=255)
                                                                                                                                                 gg‑TTTGAA‑T‑C‑GGTTTCATGCCGCCGAGCGCCAGCAGAACAATACCG‑AT‑AATG‑CCAACCAGAATTGACCAACCGATACGCACCAACAGAGGTGGTTCTTCACCATCGCGTACTTCG‑CGGCAAGTGGACATCGCCAGGGTATAAGAGCAGGCg    <  1:391612/151‑1 (MQ=255)
                                                                                                                                                    tttGAA‑T‑C‑GGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCG‑AT‑AATG‑CCAACCAGAATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCG‑CGGCAAGTGGACATCGCCAGGGTATAAGAGCAGGCGtt  >  2:688585/1‑151 (MQ=255)
                                                                                                                                                       |                                                                                                                                                          
ATCATTTAAATTAAAATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGC‑GG‑TTTGAA‑T‑C‑GGTTTCAGGCCGCCGAGCGCCAGCAGAACAATACCG‑AT‑AATG‑CCAACCAGAATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCG‑CGGCAAGTGGACATCGCCAGGGTATAAGAGCAGGCGTT  >  NC_000913/40366‑40662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑