Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,483,2680TA43.8% 137.4 / 63.7 112L24F (TTA→TTT) emrKmultidrug resistance efflux pump membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base T (40/23);  new base A (0/49);  total (40/72)
Fisher's exact test for biased strand distribution p-value = 2.47e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

GTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTG  >  NC_000913/2483118‑2483416
                                                                                                                                                        |                                                                                                                                                    
 ttCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCaataaat                                                                                                                                                      >  2:934871/1‑150 (MQ=255)
 ttCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCaataaat                                                                                                                                                      >  1:1518807/1‑150 (MQ=255)
  tCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCaataaata                                                                                                                                                     <  1:761121/150‑1 (MQ=255)
   cGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCAataaataa                                                                                                                                                    <  1:504926/150‑1 (MQ=255)
    gTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCAATAAATaaaa                                                                                                                                                  <  1:973432/151‑1 (MQ=255)
     tATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCAATAAATaaaa                                                                                                                                                  >  1:178076/1‑150 (MQ=255)
       tCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCAATAAATAAAACt                                                                                                                                                >  1:252240/1‑150 (MQ=255)
          ttATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCAATAAATAAAACt                                                                                                                                                <  2:409638/147‑1 (MQ=255)
            aTGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGcc                                                                                                                                          <  2:588817/151‑1 (MQ=255)
            aTGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGcc                                                                                                                                          <  2:1344307/151‑1 (MQ=255)
              gATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCaa                                                                                                                                        <  2:602132/151‑1 (MQ=255)
              gATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCa                                                                                                                                         >  1:1140897/1‑150 (MQ=255)
               aTTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAAt                                                                                                                                       >  1:567831/1‑151 (MQ=255)
                  accGACAGTACCACTCCCTGAGTTTTGTACAAAAATGGAATCTGCATTCCCCGTGACATAGGCGTCATCTTTACTAATCATTTTTTCAAATCCCATTGC‑CA‑AAAAGGAAAAGCCACCTAAAACCGAAATAAATAAAACTACCGCCAATaa                                                                                                                                     <  1:391259/148‑1 (MQ=255)
                   aCGACAGTGACACTCCCTGAGTTTTGTACAAAAATTGTATCTGCATTCCCCGTGACATAGGCGTCATCTTTACAAAACATGTCTTAAAATCCCATTGA‑CA‑AAAAGGAATAGGCCCCAAAAAACCAAAAAAAAAAAACTACCGCCAATAAAg                                                                                                                                   <  1:706551/151‑1 (MQ=255)
                      aCAGTGACACTACCGAAGACTTGTGAAAAAATTGGATCTGCATCCCCCGTGAAAAAGGCGTCATTTGTACAAATAATTTTTTC‑AAATCCAATGA‑CCAAAAAGAAAAAGCCCCCTAAAACCGCAAAAAAAAAAACTACCGCCAATAAAGaaa                                                                                                                                <  1:648314/151‑1 (MQ=255)
                        aGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGaaa                                                                                                                                >  1:1392937/1‑149 (MQ=255)
                             cacTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTAttt                                                                                                                         >  1:860658/1‑151 (MQ=255)
                               cTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTttc                                                                                                                       >  2:1527522/1‑151 (MQ=255)
                                   cTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTtcttct                                                                                                                   >  1:303987/1‑151 (MQ=255)
                                      ataCTTGTGAAAAAATTGAATCTGCATTCCCCGTGACATAGGCTTCATCTGTCCTAATCATTTCTTCAAATCCCATGGA‑CA‑AAAAGGCATAGGCCCCTAAAAACGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGtt                                                                                                                <  1:367349/149‑1 (MQ=255)
                                          ttGTGCAAAAATTGTATCTCCATCCCCCGTAACATAGGCGTCATCTTTACTATTCATGTCTTCTAATCCCATTCC‑CA‑AATAGGCAAAGCCACCTGAAACCGAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAg                                                                                                              <  1:1368733/149‑1 (MQ=255)
                                          ttGTGCAAAAATTGGATCTGCATCCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGGCACCTAAAAACGAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAg                                                                                                              <  2:590918/149‑1 (MQ=255)
                                          ttGTGAAGAAATTGGATCTACATCCCCCGTGACATAGGCGTCATCTTTACTAATCATTTTTTCAAATCCCATTGC‑CC‑AAAAGGAAGAGCCACTTAAAAACGAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAg                                                                                                              <  1:985953/149‑1 (MQ=255)
                                          tggggCAAAAATTGGATCTGCTTCCCCCATGACAAAGGCGTCATCTGTACAAATCATGTTTTAAAATCCCATTAC‑CC‑AATAGAAATAGCCACCTAAAACCACAAAAAAAAAAACTACCGCCAATAAAGAAAAGTAtttt                                                                                                                        <  1:997794/135‑1 (MQ=255)
                                           tgtgCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CC‑AATGGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAAt                                                                                                           >  1:145535/1‑151 (MQ=255)
                                             tgCAAAAATTGGATCTGCATTCCCCGTGACATAGGCGTCTTCTGTACTAATCATGTCTTCTAATCCCATTGC‑CC‑AATAGGCATAGGCACCTAAAACCGAAATAAAAAAACCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATg                                                                                                          <  2:947649/150‑1 (MQ=255)
                                              gaaaaaaTTGAATCTGCATTCCCCGTGACATAGGCGTCATCTGTACAAATCATGTCTTCTAATTCCATTGA‑CC‑AATGGGCATAGGCCCCAGAAACCAAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAAt                                                                                                           <  2:369282/144‑1 (MQ=255)
                                              gCAGAAATTGGATCTGCATCCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CA‑AATAGGCATAGGCCCCTAAAAACGCAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGt                                                                                                         <  2:100544/150‑1 (MQ=255)
                                                aaaatttGGATCTGCATTCCCCGTGACATAGGCTTCATCTGTACTAATCATGTCTTCAAATCCCATTGA‑CA‑AATAGGCATAGGCCCCTAAAAACGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAAt                                                                                                           <  2:9909/141‑1 (MQ=255)
                                                aaaaaTTGGATCTGCTTTCCCCGTGACATAGGCGTCATCTTTACTAATCATTTTTTTAAATCCCATTGA‑CA‑AATAGGAATAGGCTCCTAAAAACGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGt                                                                                                         <  1:1124966/146‑1 (MQ=255)
                                                aaaaaTGGGATCTGCTTCCCCCGTGACATAGGCGTCATCTGTACTAACCATGTTTTTTAATTCCATGGA‑CC‑AATAGGAATAGGCCCAAAAAACCGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGtttt                                                                                                      <  2:1133766/149‑1 (MQ=255)
                                                aGAAATTGGCTCTACGTCCCCCTTGACATGGGCCTCATCGGTATTAATCATGTTTTTAAATTCAATTGC‑CA‑AATGGGCATAGGCACCTAAAAACAAAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAAt                                                                                                           <  2:596579/146‑1 (MQ=255)
                                                aGAAATTGGATCTTTATTCCCCTTGATATTGGTGCCACATGTTCTATTCATTTATTCAAATGCCATGAA‑TG‑AATAGGCATAGGCACGTGAAAACGATAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGa                                                                                                             <  2:1455864/144‑1 (MQ=255)
                                                  aaaTTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGtttttt                                                                                                    >  1:657663/1‑151 (MQ=255)
                                                  aaaTTGGATCTGCATCCCCCGGGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CA‑AATAGGCATAGGCCCCTGAAAACGAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGtt                                                                                                        <  2:755704/147‑1 (MQ=255)
                                                      tGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATCCCATTGA‑CC‑AATAGGCATAGGCCCCTAAAAACGAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTAtt                                                                                                 <  2:1031828/150‑1 (MQ=255)
                                                       ggttCTGCATCCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTCA‑CC‑AATGGGAAAAGCCACCTGAAAACGCAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTAttt                                                                                                <  2:1557772/147‑1 (MQ=255)
                                                       ggATCTGCATCCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGGCACCTAAAAACGCAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTAtt                                                                                                 <  2:19534/149‑1 (MQ=255)
                                                        gATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTg                                                                                               >  1:667279/1‑150 (MQ=255)
                                                            tGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAAtt                                                                                           >  1:29293/1‑150 (MQ=255)
                                                              cATTCCCCGTGACATAGGCGTCATCTGTACTAAACATCTTTTATAATTCCATTGA‑CA‑AATAGGAAAAGGACCATTAAAACAAAAAAAAAAAATCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAAt                                                                                            <  1:534488/147‑1 (MQ=255)
                                                               aTTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATc                                                                                       >  2:162813/1‑151 (MQ=255)
                                                               aTTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATc                                                                                       >  2:1056093/1‑151 (MQ=255)
                                                                tcccccGTGACATAGGCGTCATCTGTACTAATCATGTCTTCAAATTCCATTGA‑CC‑AATAGGCATAGGCACCTAAAAACGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAAt                                                                                        <  2:874129/147‑1 (MQ=255)
                                                                tcccccGTGAAATAGGCTTCATTTGTACAAAACATGTTTTCAAATTCAATTGC‑CA‑AATAGCAATAGCCACATAAAAACGAAAAAAAAAAAATTTCCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTaa                                                                                         <  1:399291/146‑1 (MQ=255)
                                                                 tCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTg                                                                                     >  2:807302/1‑151 (MQ=255)
                                                                  ccccGTGACATGGGCGTCATTTTTACTAATAATTTCTTCAAATTCAATTGA‑CC‑AAAAGGAATAGGCCTCTAAAAACCAAATAAAAAAAATTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTg                                                                                     <  1:379247/150‑1 (MQ=255)
                                                                  ccccGTGACATGGGCGTCATCTGTACTAATCATGTCTTCTAATCCCATTGA‑CC‑AATAGTCATAGGCCCCTGAAAACTCAAAAAAAAAACCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGt                                                                                    <  2:1396703/151‑1 (MQ=255)
                                                                  ccccGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATCCCATTGC‑CC‑AATAGGAATAGGCACCTGAAAACGAAATAAAAAAATCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGa                                                                                              <  1:37520/141‑1 (MQ=255)
                                                                  ccccGTGACATAGGCGTCATCTGTACAAATAATTTCTTATAATTCCATTGA‑CC‑AATAGAAATAGGCCCCTAAAAACGAAAAAAAAAAAATTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATc                                                                                       <  1:610309/148‑1 (MQ=255)
                                                                  ccccGTGACAAGGGCGTCATCTTTATAAATCATGTCTTCAAATCCCATTGC‑CC‑AATAGAAATAGGCACTTAAAACCGAAAAAAAAAAACCTTCCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCt                                                                                      <  1:934769/149‑1 (MQ=255)
                                                                    ccGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTc                                                                                  >  2:1240516/1‑151 (MQ=255)
                                                                      gTGACATAGGCGTCTTCTGTATTAATCATTTTTCCAAATTCCATTCA‑CC‑AATGGCCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCa                                                                                <  2:816632/151‑1 (MQ=255)
                                                                      gTGACATAGGCGTCATCTGTACAAATAATGTCTTCTAATCCCATTGA‑CC‑AATAGGAATAGACACCTGAAAACGAAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCa                                                                                <  1:586410/151‑1 (MQ=255)
                                                                        gACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGGCCCCTAAAAACGCAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCAc                                                                               <  2:205709/150‑1 (MQ=255)
                                                                         aCATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACt                                                                              >  2:1511946/1‑150 (MQ=255)
                                                                           aatggCTTCATCTGTAATAACCATGTCTTATAATCCCATTAA‑CA‑AATCGGGATAGGCACAAGAAACCGCAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCa                                                                                <  2:534519/143‑1 (MQ=255)
                                                                              ggCGTCATCTTTACTAATCATGTCTTCAAATTCCATTGC‑CA‑AAAAGGCAAAGGCCCCTAAAAACGAAAAAAAAAAATCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACtatta                                                                          <  1:738303/149‑1 (MQ=255)
                                                                              ggCGTCATCTGTACTAATCATTTTTTCAAATCCCATTGC‑CA‑AAAAGGCAAAGGCACCTAAAAACGAAATAAAAAAAATTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACtatt                                                                           <  1:891206/148‑1 (MQ=255)
                                                                               gCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTAtct                                                                       >  1:903002/1‑151 (MQ=255)
                                                                                 gTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTAtctct                                                                     >  1:1314533/1‑151 (MQ=255)
                                                                                  tCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTAtctctc                                                                    >  1:708175/1‑151 (MQ=255)
                                                                                        gTACTAATCATGTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTtct                                                              >  2:541164/1‑151 (MQ=255)
                                                                                         tACTAATCATGTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTtctc                                                             >  2:891715/1‑151 (MQ=255)
                                                                                             aaTCATGTCTTCTAATCCCATTGC‑CA‑AATAGGCATAGGCACCTAAAAACGCAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCAttt                                                                <  2:1512226/144‑1 (MQ=255)
                                                                                                  tGTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGa                                                     >  1:1122817/1‑150 (MQ=255)
                                                                                                  tGTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGCCACCTGAAAACGCAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAg                                                         <  2:259042/146‑1 (MQ=255)
                                                                                                   gTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGaa                                                    >  2:1181674/1‑150 (MQ=255)
                                                                                                        ctAATTCCATTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAt                                              >  1:1182840/1‑151 (MQ=255)
                                                                                                         tAATTCCATTGA‑CC‑AATAGGCATAGGCGCCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtg                                             >  2:41318/1‑151 (MQ=255)
                                                                                                          aaTTCCATTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtg                                             >  1:1450695/1‑150 (MQ=255)
                                                                                                           aatCCAATGACCC‑AAAAGGAATAGTCACGTAAAACCACAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATa                                                          <  1:514088/135‑1 (MQ=255)
                                                                                                              ccATTGA‑CC‑AATAGGCATAGGCACCTAAAAACGCAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaat                                          <  2:376026/149‑1 (MQ=255)
                                                                                                              ccATTGA‑CA‑AATAGGCAAAGGCCCCTAAAAACGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaattg                                        <  1:480497/151‑1 (MQ=255)
                                                                                                               cATTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaattg                                        >  2:1361898/1‑150 (MQ=255)
                                                                                                                actGA‑CC‑AAAAGACGTATTCACCTATAATCACAATAAATAATTATACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTAGCCCTCATTTCTCAGAGATGAAATTTAtgaattgaa                                      <  1:793786/149‑1 (MQ=255)
                                                                                                                aTTGA‑CC‑AATAGGCATAGGCACTTAAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaatt                                         <  2:444168/148‑1 (MQ=255)
                                                                                                                aTTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaattga                                       >  2:621086/1‑150 (MQ=255)
                                                                                                                 ttGA‑CA‑AATAGGCATAGGCACCTAAAACCGAAAAAAATAAACCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaatt                                         <  1:1562072/147‑1 (MQ=255)
                                                                                                                       caaaTAGGCATAGCCCCCTGAAAACCAAAAAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATtgaatgaa                                  <  2:159542/148‑1 (MQ=255)
                                                                                                                       c‑AATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGaaa                                 >  2:1233586/1‑150 (MQ=255)
                                                                                                                         aaaaggaataggccCCTAAAAACGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATCATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGaaa                                 <  1:1055981/146‑1 (MQ=255)
                                                                                                                         aaTAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGaaa                                 >  2:895040/1‑149 (MQ=255)
                                                                                                                            aggcatagccaCCTAAAAACAAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAagag                             <  1:1520220/150‑1 (MQ=255)
                                                                                                                             ggaataggctCCAAAAAACGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGca                           <  1:1429960/148‑1 (MQ=255)
                                                                                                                             ggaataggcaCCTGAAAACGAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGc                            <  2:1159009/147‑1 (MQ=255)
                                                                                                                             ggaatagccaCCTGAAAACGAAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAagag                             <  2:267251/146‑1 (MQ=255)
                                                                                                                                atagcccCCTAAAACCGAAAAAAATAAATCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGc                            <  1:1202159/147‑1 (MQ=255)
                                                                                                                                  agcctccTGAAAACGAAAAAAATAAAACTTCCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGcatt                         <  1:443993/143‑1 (MQ=255)
                                                                                                                                       ccTGAAAACGAAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCAttctt                  <  2:1241026/150‑1 (MQ=255)
                                                                                                                                       ccTAAAACCGAAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCAttcttc                 <  2:519976/151‑1 (MQ=255)
                                                                                                                                       ccTAAAACCGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCAttctt                  <  2:1500369/150‑1 (MQ=255)
                                                                                                                                        cTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCAttcttc                 >  1:1032278/1‑150 (MQ=255)
                                                                                                                                         taaaacCGAAAAAAATAAAATTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCAttct                   <  1:1250023/145‑1 (MQ=255)
                                                                                                                                          aaaacagaaataaaaaAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCat               <  2:2561/142‑1 (MQ=255)
                                                                                                                                           aaaaCGAAAAAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCatat             <  1:636899/151‑1 (MQ=255)
                                                                                                                                           aaaaCCCAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCata              <  2:1393073/150‑1 (MQ=255)
                                                                                                                                                  aataaataAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAacac      >  2:1408459/1‑151 (MQ=255)
                                                                                                                                                  aataaaaaAACTTTCCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCaaa         <  1:93008/148‑1 (MQ=255)
                                                                                                                                                        |                                                                                                                                                    
GTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CC‑AATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTG  >  NC_000913/2483118‑2483416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑