Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,676,1060CA47.5% 105.6 / 68.7 101L240F (TTG→TTT) yphDputative sugar ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (44/9);  new base A (0/48);  total (44/57)
Fisher's exact test for biased strand distribution p-value = 5.17e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

AGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTGATGA  >  NC_000913/2675957‑2676254
                                                                                                                                                       |                                                                                                                                                    
aGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCcaaca                                                                                                                                                     <  2:233234/151‑1 (MQ=255)
aGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCcaac                                                                                                                                                      <  1:526352/150‑1 (MQ=255)
aGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCcaac                                                                                                                                                      >  2:779733/1‑150 (MQ=255)
  cGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCcaacaa                                                                                                                                                    >  2:890095/1‑150 (MQ=255)
   gTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCCAACaaa                                                                                                                                                   >  1:1379763/1‑150 (MQ=255)
     aaTCACCAGCACACCAAGCAAGGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCCAACAAAAt                                                                                                                                                 <  2:1353621/150‑1 (MQ=255)
          ccAGCACACCAAGCAATGTGCCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCCAACAAAATGCCgg                                                                                                                                            <  1:38107/150‑1 (MQ=255)
           cAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCCAACAAAATGCCGGTc                                                                                                                                          <  1:407782/151‑1 (MQ=255)
               acacCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCCAACAAAATGCCGGTCAccg                                                                                                                                      >  2:941620/1‑151 (MQ=255)
               acacCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCCAACAAAATGCCGGTCAccg                                                                                                                                      >  2:849426/1‑151 (MQ=255)
                cacCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCCAACAAAATGCCGGTCAccgc                                                                                                                                     >  2:1090124/1‑151 (MQ=255)
                cacCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCCAACAAAATGCCGGTCAccgc                                                                                                                                     >  2:30823/1‑151 (MQ=255)
                  ccAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCCAACAAAATGCCGGTCAccgcc                                                                                                                                    <  2:29431/150‑1 (MQ=255)
                       cAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCCAACAAAATGCCGGTCACCGCCGCtaat                                                                                                                              >  1:114663/1‑151 (MQ=255)
                       cAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCCAACAAAATGCCGGTCACCGCCGCtaat                                                                                                                              >  2:468628/1‑151 (MQ=255)
                          tGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCCAACAAAATGCCGGTCACCGCCGCTaataat                                                                                                                           >  1:1502792/1‑151 (MQ=255)
                           gTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATc                                                                                                                          >  2:190676/1‑151 (MQ=255)
                              ccaaaCAGGGACCCGCGCCCGCCGGAAAGTGCCTTCCCGCCGCCCACGCCCGCGGCGATGACGTCAAACCCCAGCCCGTTTGCGG‑CCCCTGCGTTCCCAGACCCGAGGC‑GCGCCGCAAAAAAAATGCCGGTCACCGCCGCTAATAATcc                                                                                                                         <  2:1119141/146‑1 (MQ=255)
                                 acaggggACCCGCCCCCCCCGGAAGGTCCCGTCCCCCCGCCCACGCCCGGGGCGATGCGGTCAACCCCCAGCCGTTTTGCGG‑CCCCTGCGTTACCAGACCCGGGGC‑GCGCCCCCAAAAAAATGCGGGTCACCGCCGCTAATAATCCCGaaa                                                                                                                    <  2:554412/147‑1 (MQ=255)
                                 aaCGGGGACCCGCGCCCCCCGGAAGGTGCCGTCCCCCCGCCCACGCCCGGGGCGATGACGTCAACCCCCAGCCCGTTTGCGG‑CCCCTGCGTTCCCAAAACCGGGGC‑GCGCCCCAAAAAAAATGCCGTTCACCGCCGCTAATAATCCCGaa                                                                                                                     <  1:1456805/150‑1 (MQ=255)
                                   cAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAgg                                                                                                                  >  1:141133/1‑151 (MQ=255)
                                     gggACCCGCGCCCGCGGAAAAGGGCCGTCCCCCCGCCCACGCCCGCGGCGATGACGTCAAACCCCAGCCCGTTTGCGC‑CCCCTGCGTTACCAAAACCGAGGC‑GCGCCGCCAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAg                                                                                                                   <  1:1460612/148‑1 (MQ=255)
                                      gacccccggccggccgAAACGTGCCTCCCCGCCGCCCACGCCCGCGGGGAGGACGTCAACCCCCAGACCTTTTGCGG‑CCCCTGCGTTACAAGAACCGGGGG‑GCGCCCCCAAAAAAATGCGGTTCACCGCCGCTAATAATCCCGAAAg                                                                                                                   <  1:1401280/135‑1 (MQ=255)
                                          ccgcccccgccgGAAAGGGCCGTCCCGCCGCCCCCGCCCGCGGCGATGACGACAACCTCCAGCCCGTTTGCGG‑CCCCTGCTTTACCAGAACCGGGGG‑GCGCCGCAAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAg                                                                                                            <  1:440962/144‑1 (MQ=255)
                                          ccGCGCCCGCCGAAAGGCGCGGTCCCCCCACCCACGCCCGCGGCAATGACGTCAAACTCCAGCCCTTTGGGGG‑CACTGGCGTTACCAAAACGGAGGC‑GCGCCGCAAAAAAAATGCGGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGa                                                                                                           <  1:221752/151‑1 (MQ=255)
                                          ccGCGCCCGCCGAAAAGTGCCGTCCCGCCGACCACGCCCGCGGCGATGACGTCAACCCCCAGACCTTTGGCGG‑CCCCTGCGTTACCAAAACGGAGGC‑GCGCGGCAAAAAAAAGGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGa                                                                                                           <  1:915315/151‑1 (MQ=255)
                                          ccGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTaa                                                                                                              >  1:26439/1‑148 (MQ=255)
                                            gcgACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGAt                                                                                                          >  1:634637/1‑150 (MQ=255)
                                             cgACCGCCGGAAAGGGCCGTACCGCCGACCACGCCCGCGGCGATGACGTAAAACCCCAGCCCGTTTGCGG‑CCCCTGCGTTCCCAGAACCGAGGC‑GCGCCGCAAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATa                                                                                                         <  2:1554432/150‑1 (MQ=255)
                                                 cgccgGAAAGGGCCGCCCCGCCGACCACGCCCGCGGCGATGCCGTAAACCCCCAGCCCGTTGGCGC‑CCCCTGCGTTACCAAACCGGAGGC‑GCGCCGCAAAAAAAATGCGGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAAt                                                                                                    <  1:1068218/151‑1 (MQ=255)
                                                   cgggAAAGTGCCGTCCCGCCGCCCACGCCCGCGGCGATGACGTAAACCCCCAGCCCGTTTGCGG‑CACCTGCGTTACCAAAACCGAGGC‑GCGCCGAAAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAAt                                                                                                    <  1:805573/147‑1 (MQ=255)
                                                    cgGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATgcg                                                                                                 >  1:708170/1‑151 (MQ=255)
                                                    cgGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATgcg                                                                                                 >  2:1209472/1‑151 (MQ=255)
                                                     ggAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATgcgc                                                                                                >  2:1003571/1‑151 (MQ=255)
                                                     gaaaaGTGCCGTCCCGCCGCCCCCGCCCGCGGCGATGACGTCAAACCCCAGCCCTTTTGCGG‑CCCCTGCGTTACCAGACCCGAGGC‑GCGCCGCCAAAAAAAGGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATgcgc                                                                                                <  2:1521038/149‑1 (MQ=255)
                                                       aaaGTGCCGTACCGCCGCCCACGACCGCGGCGATGACGTCAAACTCCAGCCCGTTTGCGG‑CACCGGCTTTACCAGAACCGAGGC‑GCGCCGCAAACAAAATGCGGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATgcg                                                                                                 <  1:1013036/148‑1 (MQ=255)
                                                       aaaGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCAc                                                                                              >  1:882780/1‑151 (MQ=255)
                                                       aaaGCGCCGTCCCGCCGCCCCCGCCCGGGGCGATGACGTCAACCCCCACGCCGTTGGCGG‑CCCCGGCGTTACCAAAACCGAGGC‑GCGCGGCAAAAAAAATGCCGGTAACCGCCGCTAATAATCCCGAAAGGGTAAAGATaa                                                                                                        <  1:306877/141‑1 (MQ=255)
                                                        aaGTGCGGTACCGCCGACCCGGCCCGCGGCGATGCCGCAAAACACCAGACGGTTGGCGG‑CGCCGGCTTTCCCAGACCCGAGGC‑GGGCCGCAAAAAAAATGCCGGTAACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCa                                                                                               <  2:1445473/149‑1 (MQ=255)
                                                         aGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCAccc                                                                                            >  2:18227/1‑151 (MQ=255)
                                                           tGCCTTCCCGCGAACCACGCCCGGGCGGATGCCGTCAAACTCCAACCCTTTGGCGG‑CCCCTGCGTTACCAAACCGGAGCC‑GCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCcg                                                                                           <  2:1258154/150‑1 (MQ=255)
                                                           tGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCcg                                                                                           >  1:1066277/1‑150 (MQ=255)
                                                             ccGTCCCGCCGCCCACGCCCGGGGCTATGACGTCAACCTCCAGACCGTTGGCGG‑CACCTGCGTTCCCAGAACGGAGGC‑GCGCCCCCAAAAAAAGGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCcgacg                                                                                        <  2:205048/151‑1 (MQ=255)
                                                             ccGTACCGCCGCCCACGCCCGCGGCGATGACGTAAACCCCCAGCCCGTTGGCGG‑CACCTGCTTTACCAAACCCGAGGC‑GCGCCGCAAAAAAATTGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCcgacg                                                                                        <  2:943590/151‑1 (MQ=255)
                                                              cGTCCCGCCGCCCACGCCCGCGGCGATGACGTCAACCCCCAGCCCGTTTGCGG‑CCCCTGCTTTCCAAGACCCGAGGC‑GCGCCGCCAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCcgacga                                                                                       <  2:819749/151‑1 (MQ=255)
                                                                  ccgccgCCCACGCCCGCGGCGAGGACGTAAACCCCCAGACCGTTGGCGG‑CACCGGCGTTACCAGAACCGAGGC‑GCGCCGCAAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGaa                                                                                      <  1:712203/148‑1 (MQ=255)
                                                                  ccgccgACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGt                                                                                   >  2:451084/1‑151 (MQ=255)
                                                                  ccccccaCCACGCCCTCGGCGATGCCGCAAAACCCCAGACTTTTTGGGC‑CCCCGGCTTTCCCAGACCCGAGGC‑GCGCCCCCAAAAAAAGGCCGGCCTCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAAc                                                                                     <  1:1426675/143‑1 (MQ=255)
                                                                   cgccgACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGtt                                                                                  >  1:371719/1‑151 (MQ=255)
                                                                     ccgcccccgcccGCGGCGAGGCCGCCAACCCCCACACCTTTTGCGG‑CACCCGCGTTCCCAAACCAGAGCC‑GCGCCGCAAAAAAAAGGCCGCTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGaa                                                                                      <  1:553149/145‑1 (MQ=255)
                                                                      cgACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGAt                                                                               >  2:89652/1‑151 (MQ=255)
                                                                      cgACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCAGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGAAAAGATAAGAATGCGCACCCGACGAACGTTg                                                                                 >  2:502156/1‑149 (MQ=255)
                                                                         ccccgcccGCGGCGAGGCCGTCAACCCCCAGCCCGTTTGCGC‑CCCCTGCTTACCCAGAACCGGGGC‑GCGCCGCAAAAAAAGGGCCGGCCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATg                                                                              <  1:811477/143‑1 (MQ=255)
                                                                         ccACGCCCGCGGCGATGACGCCAACCCCCAGCCCGTTGGCGG‑CCCCGGCGTTACCAGAACCGGGGC‑GCGCCGCAAAAAAAAGGCCGGCCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGaa                                                                                      <  1:492902/141‑1 (MQ=255)
                                                                          cACGCCCGCGGCGATGACGTAAACCCCCAGACCGTTGGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCAAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCg                                                                           <  2:302220/151‑1 (MQ=255)
                                                                             gACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGcac                                                                        >  2:1111394/1‑151 (MQ=255)
                                                                             gACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGcac                                                                        >  1:554445/1‑151 (MQ=255)
                                                                               cggcggcgAGGGCGTCAACCCCCAGACGTTTTGCGG‑CCCCTGCGTTACAAAAGCCGAGGG‑GCGCCGCAAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGcac                                                                        <  1:1237115/147‑1 (MQ=255)
                                                                                 gcggggcagactcaaaaCCCCAGACCTTTGGCGC‑CCCCTGCGTACCCAAACCCGAGGC‑GCGCCGCCAAAAAAGGGCCTGCCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGcac                                                                        <  1:1086018/133‑1 (MQ=255)
                                                                                 gcggcgATGACGTCAAACTCCAGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACt                                                                    >  2:885875/1‑151 (MQ=255)
                                                                                   ggcgATGCCGTCAACCCCCAGCCCGTTGGCGG‑CCCCTGCGTTCCCAAACCCGAGGC‑GCGCCGCAAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACt                                                                    <  1:1433049/149‑1 (MQ=255)
                                                                                   ggcgATGACGTCAAACTCCAGCCCGTTTGCGG‑CCCCTGCTTTCCCAGACCCGAGGC‑GCGCCCCAAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAAc                                                                     <  2:124590/148‑1 (MQ=255)
                                                                                   ggcgATGACGTCAAACTCCAGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGCCGACCGTTGATGCCGCACAACt                                                                    >  1:1120835/1‑149 (MQ=255)
                                                                                        ggACGTCAACCCCCAGCCCGTTGGCGG‑CCCCTGCGTTCCAAAAACCGAGGC‑GCGCCGCAAAAAAAAGGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGcgccgc                                                             <  1:409531/150‑1 (MQ=255)
                                                                                            gTCAAACTCCAGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGCTGATGCCGCACAACTGCGCCGCCGt                                                          >  2:1015029/1‑150 (MQ=255)
                                                                                               aacccccAGCCCTTTTGCGG‑CCCCTGCTTTCCCAGACCCGGGGC‑GCGCCGCAAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTa                                                         <  2:1137151/143‑1 (MQ=255)
                                                                                               aacccccAGCCCGTTTGGGG‑CCCCGGCGTTCCCAGACCCGAGGC‑GCGCCCCAAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTa                                                         <  2:658231/143‑1 (MQ=255)
                                                                                                acccccAGCCCGTTTGCGG‑CCCCTGCGTTCCCAGAACCGGGGC‑GCCCCCCAAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCAt                                                     <  2:496657/147‑1 (MQ=255)
                                                                                                  cccccagccGTTTGGGGCCCCCGGCGTT‑CCAAAACCGGGGCGGCCCCCCAAAAAAAACGCGGGCCCCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCAtt                                                    <  1:999375/144‑1 (MQ=255)
                                                                                                  ccccAGCCCGTTTGCGG‑CCCCTGCTTTACCAGACCCGAGGC‑GCCCCGCCAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCAtt                                                    <  2:1331352/148‑1 (MQ=255)
                                                                                                   ccaggACCGTTGGCGC‑TCCCTGCGTACAAAGAACAAAGGC‑GCGCGAACACAAAACTGCGGGCAACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTa                                                   <  1:853305/146‑1 (MQ=255)
                                                                                                    ccAGCCGGTTTGCGC‑CCCCGGCGTCACCAGACCCGGGCC‑CCCCCGCCAAAAAAAGCCGGGCCCCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTAcc                                                 <  1:277602/151‑1 (MQ=255)
                                                                                                      aGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGc                                               >  2:1060911/1‑151 (MQ=255)
                                                                                                            tttGCGC‑CACCTGCTTTCCAAGACCGGAGGC‑GCCCCCCAAAAAAAATGCCGTCCCCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACg                                          <  1:475195/150‑1 (MQ=255)
                                                                                                              tggcgcccccggCGTTACCAAACCGGGGGC‑GCGCCCCCAAAAAAAGGCGGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACgg                                         <  1:307445/139‑1 (MQ=255)
                                                                                                                     aCCTGCGCTACCAGAACCGAGGC‑GCGCGGCCAACAAAATGCCGGTTACCGCCGCCAATAATCCCGAAAGGGACAAGATAAGAATGCGCACCCCACAAACGTTCAAGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAAcc                                 >  1:1210895/1‑151 (MQ=255)
                                                                                                                       gtggttttcaaGAACCGGGGC‑GCGCCGCTAAAAAAGGGCGGGGCCCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAGCTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCg                                <  2:1411383/140‑1 (MQ=255)
                                                                                                                          cGTTACCAGAACCGAGGC‑GCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAgcg                            >  2:701519/1‑151 (MQ=255)
                                                                                                                            ttacaaaacccGAGGC‑GCCCCCCCAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGa                               <  2:800707/137‑1 (MQ=255)
                                                                                                                             tACCAGAACCGAGGC‑GCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGCACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGccc                         >  1:1553996/1‑151 (MQ=255)
                                                                                                                             tACCAGAACCGAGGC‑GCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGccc                         >  2:907607/1‑151 (MQ=255)
                                                                                                                                cAGAACCGAGGC‑GCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGa                       >  2:149638/1‑150 (MQ=255)
                                                                                                                                 aGACCCGAGGC‑GCGCCCCAAACAAAACGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACTTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAgcgc                           <  2:626296/145‑1 (MQ=255)
                                                                                                                                 aGAACCGAGGC‑GCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGa                       >  2:1328838/1‑149 (MQ=255)
                                                                                                                                  gAACCGAGGC‑GCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAgg                    >  2:394225/1‑151 (MQ=255)
                                                                                                                                    aCCGAGGC‑GCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCg                  >  2:527324/1‑151 (MQ=255)
                                                                                                                                     cggggcc‑gcacCGCCACAAAAAGCCCGGCCACCGCCGCTAATAAGCCCGAAAGGGTAAAAATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGaa                      <  1:1014923/140‑1 (MQ=255)
                                                                                                                                     ccGGGGC‑GCCCCCCAAAAAAAAGGCCGGCCCCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGc                   <  1:398881/149‑1 (MQ=255)
                                                                                                                                     ccGAGGC‑GCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCgg                 >  1:956235/1‑151 (MQ=255)
                                                                                                                                      ccggggcagcccgcaAAAAAAATACCGGCCCCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCAACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGc                   <  1:548451/139‑1 (MQ=255)
                                                                                                                                      cGAGGC‑GCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCgg                 >  2:1469975/1‑150 (MQ=255)
                                                                                                                                       gggcc‑gcccCGCAAAAAAAAGGCCGTTCCCCGCCGCTAATAATCCCGAAAGGTTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGtt               <  2:689234/147‑1 (MQ=255)
                                                                                                                                       gAGGC‑GCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGtt               >  2:1265755/1‑151 (MQ=255)
                                                                                                                                         ggC‑GCCCCCCAAAAAAAATGCGGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGttt              <  2:498668/150‑1 (MQ=255)
                                                                                                                                           c‑gcgcCCCCAAAAAAATGCGGGTCCCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTg            <  2:1557668/150‑1 (MQ=255)
                                                                                                                                             gcgcCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCg          >  1:227136/1‑151 (MQ=255)
                                                                                                                                              cgccgcCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCgg         >  1:1469967/1‑151 (MQ=255)
                                                                                                                                              cgccccAAAAAAAGTGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGc           <  2:1066391/149‑1 (MQ=255)
                                                                                                                                                ccgcaAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCt       <  2:861331/151‑1 (MQ=255)
                                                                                                                                                     aacaaAGTGCCGGTCCCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCtgatga  <  1:680394/151‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                    
AGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGG‑CACCTGCGTTACCAGAACCGAGGC‑GCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTGATGA  >  NC_000913/2675957‑2676254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑