Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,346,6750TG42.6% 183.8 / 22.1 108T218P (ACC→CCC) fumBanaerobic class I fumarate hydratase (fumarase B)
Reads supporting (aligned to +/- strand):  ref base T (14/48);  new base G (46/0);  total (60/48)
Fisher's exact test for biased strand distribution p-value = 3.06e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

AACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAGGTCG  >  NC_000913/4346527‑4346823
                                                                                                                                                    |                                                                                                                                                    
aaCGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTAc                                                                                                                                                    <  1:439568/151‑1 (MQ=255)
 aCGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGGCGGCTGCCGGAACGTGGGGACg                                                                                                                                                   >  1:1157772/1‑151 (MQ=255)
 aCGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACg                                                                                                                                                   <  2:349415/151‑1 (MQ=255)
    ccTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGGAGGCCGGAGTACCGGGGGTAAGCAt                                                                                                                                                >  1:1017765/1‑151 (MQ=255)
             gttcgttcCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGAAGCATTTTCTcggc                                                                                                                                       >  2:643604/1‑149 (MQ=255)
              ttcgttcCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACGCATTTTTTcga                                                                                                                                        >  2:542902/1‑149 (MQ=255)
               tcgttcCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTcgacg                                                                                                                                      <  1:435078/150‑1 (MQ=255)
                cgttcCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTTTcggc                                                                                                                                       >  2:206157/1‑146 (MQ=255)
                cgttcCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTcgacga                                                                                                                                     <  2:1366640/150‑1 (MQ=255)
                 gttcCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAgg                                                                                                                                   <  2:834792/151‑1 (MQ=255)
                        tCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCACAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGtt                                                                                                                              <  2:1072715/149‑1 (MQ=255)
                         ccGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGGTGCAGTACCGAGGGGAAGCATTTTCTCGCCGAGGGAGttttt                                                                                                                           >  1:142267/1‑151 (MQ=255)
                          cGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGTCAGGGTGCAGTAACCAGGGTATGCATTTTTTCGACGGGGAAGttttt                                                                                                                           >  2:817155/1‑150 (MQ=255)
                           gTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCCGGGCGGACTCCCGGGGGGAGGCATTTTTTCGGCGAGGGAGgtttt                                                                                                                           >  1:300150/1‑149 (MQ=255)
                            tCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCACGCTGCAGGACCGCGGGGAAGCATTTTTTCGACGAGGGAGgtttt                                                                                                                           >  2:1413111/1‑148 (MQ=255)
                             cGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAg                                                                                                                        <  2:1211587/150‑1 (MQ=255)
                              ggCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCGATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAg                                                                                                                        <  1:742726/149‑1 (MQ=255)
                              ggCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGtt                                                                                                                      <  1:731034/151‑1 (MQ=255)
                               gCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGGAGAACCCAGGGGACGCATTTTCTCGACGAGGAAGGTTTTTAGttt                                                                                                                     >  2:795334/1‑151 (MQ=255)
                               gCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACGCATTTTTTCGACGAGGGAGTTTTTCAGttt                                                                                                                     >  2:1134632/1‑151 (MQ=255)
                                cAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCGCGCTGCAGTACCGAGGGGCCGCACTTTTTTGACGGGGGAGttttttactt                                                                                                                      >  1:263625/1‑144 (MQ=255)
                                cAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTg                                                                                                                    <  1:831720/151‑1 (MQ=255)
                                cAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTg                                                                                                                    <  2:288727/151‑1 (MQ=255)
                                 aGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGc                                                                                                                   <  2:339548/151‑1 (MQ=255)
                                    tCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCg                                                                                                                 <  1:956627/150‑1 (MQ=255)
                                     cATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCACAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCggg                                                                                                               <  1:579861/151‑1 (MQ=255)
                                      aTCGTAATAGTGAGCGCTTGCTGACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGGAACGAGGGGAAGCATTTTTTCGCCGAGGGAGGTTTTTAGTTTGCCggg                                                                                                               >  2:910070/1‑150 (MQ=255)
                                      aTCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCGGGGGGCAGTACCGAGGGGACGCCTTTTTTTTACGAGGGAGTTTTTTTGTTTGCCgggg                                                                                                              >  1:24466/1‑150 (MQ=255)
                                       tCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCCGGCGGCAGGACCGAGGGGACGCATTTTTTCCACGGGGGAGGTTTTTCGTTTTCCggg                                                                                                               >  1:1030802/1‑149 (MQ=255)
                                       tCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGCGGGGACGCATTTTCTCGACGAGGAAGTTTTTTAGTTTTCCggggg                                                                                                             >  2:406982/1‑149 (MQ=255)
                                        cGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACGCATTTTTTCGACGAGGGAGGTTTTTAGTTTTCCggggg                                                                                                             >  2:791058/1‑148 (MQ=255)
                                           aaTAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTca                                                                                                          <  2:724645/150‑1 (MQ=255)
                                           aaTAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACGCCTTTTTTCGACGAGGAAGGTTTTCAGTTTTCCGGGAggc                                                                                                           >  2:891829/1‑147 (MQ=255)
                                           aaTAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACGCATTTTTTCGACGAGGAAGGTTTTTAGTTTGCCggggg                                                                                                             >  2:553885/1‑145 (MQ=255)
                                             tAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGTAGGCCGCAGCACCGAGGGTATGCATTTTTTCGACGAGGAGAGTTTTCCGTTTGCCGGGGGGcag                                                                                                         >  2:249997/1‑149 (MQ=255)
                                             tAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGGACCGAGGGGACGCATTTTCTCGACGAGGAAGGTTTTCAGTTTGCCGGGGGTcagc                                                                                                        >  2:368384/1‑150 (MQ=255)
                                              aGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCCGGCTGCAGTACCGAGGGGACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAgggagc                                                                                                        >  2:1343945/1‑144 (MQ=255)
                                              aGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACGCATTTTTTCGACGAGGAAGTTTTTTAGTTTGCCGGGGGTcagc                                                                                                        >  2:1114032/1‑149 (MQ=255)
                                                 gAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAggg                                                                                                    <  1:582589/150‑1 (MQ=255)
                                                 gAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGc                                                                                                   <  2:791592/151‑1 (MQ=255)
                                                     gcTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTg                                                                                               <  1:1511824/151‑1 (MQ=255)
                                                      cTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCCAGGGTACGTATTTTTTCGACGAGGGAGTTTTTTTGTTTTCCGGGGGTCAGCAGGGCttt                                                                                                >  2:1501579/1‑149 (MQ=255)
                                                       ttGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACGCATTTTTTCGACGAGGAAGGTTTTTAGTTTTCCGGGGGGCAGCAGGGGttt                                                                                                >  2:1006869/1‑148 (MQ=255)
                                                        tGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTgg                                                                                              <  1:1017937/149‑1 (MQ=255)
                                                          cTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGGAGGCTGGAATACCGAGGGGACGCCTTTTTTAGACGAGGGAGGTTTTTCGTTTTCCGGGGGGCAGCAgggggtttggt                                                                                            >  1:1403101/1‑141 (MQ=255)
                                                            aaCTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGGAAGCTGCAGTAACGAGGGGGAGCATTTTCTCGACGAGGGAGGTTTTTAGTTTGCCGGGAGGCAGCAGGGGTTTGGgtttc                                                                                         >  2:955746/1‑148 (MQ=255)
                                                            aaCTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTc                                                                                          <  2:1139271/149‑1 (MQ=255)
                                                             aCTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTGCCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTg                                                                                       <  2:298318/151‑1 (MQ=255)
                                                             aCTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCCGGCTGCCGTACCGAGGGGACGCATTTTTTCGCCGAGGGAGGTTTTTAGTTTGCCGGGGGGCAGCAGGggtttggttttc                                                                                         >  1:1145371/1‑147 (MQ=255)
                                                             aCTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCCGGCGGCAGTAACCAGGGTACGCCATTTTTCGCCGAGGGAGTTTTTCAGGTTGCCGGGAGGCAGCCGGGCTTTGGGTTTCt                                                                                        >  1:788687/1‑150 (MQ=255)
                                                               ttGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCCGCAGTACCGAGGGGACGCATTTTTTCGACGAGGAAGTTTTTTAGTTTGCCGGGGGTCAGCAGGGCTTTTGTTTTCTgg                                                                                      >  2:1384898/1‑150 (MQ=255)
                                                                tGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGGTGCAGGACCGAGGGGACGCCTTTTTTCGACGGGGGAGGTTTTCAGTTTGCCGGGGGTCAGCAGGGCTTTGGgtttc                                                                                         >  1:254273/1‑144 (MQ=255)
                                                                 gACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTa                                                                                    <  2:1222606/150‑1 (MQ=255)
                                                                 gACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTa                                                                                    <  1:270082/150‑1 (MQ=255)
                                                                 gACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACGCATTTTCTCGACGAGGGAGGTTTTCAATTTTCCGGGGGTCAGCAGGGCTTTGGGTTTCttg                                                                                      >  2:383710/1‑146 (MQ=255)
                                                                  aCGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTAACGAGGGGACGCATTTTCTCGACGAGGAAGTTTTTTAGTTTGCCGGGAGGCAGCAGGGCTTTGGGTTTCTgg                                                                                      >  2:1330577/1‑147 (MQ=255)
                                                                    ggTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCCGGGGGCAGGACCGAGGGGAAGCATTTTTTCGACGAGGAAGGTTTTTAGTTTTCCGGGGGGCAGCAGGGGTTTGGTTTTCTgg                                                                                      >  1:970108/1‑145 (MQ=255)
                                                                          cAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGGCGGGTGCAGTACCGAGGGGACGCATTTTTTCGACGAGGGAGGTTTTTAGTTTTTCGGGGGGCAGCAGGGGTTTTGGTTTCTCGTAGAGATaactt                                                                          >  1:512560/1‑147 (MQ=255)
                                                                          cAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGtt                                                                          <  2:373626/151‑1 (MQ=255)
                                                                           aGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCCGCAGTACCGAGGGGACGCCTTTTTTCGACGAGGAAGTTTTTTCGTTTTCCGGGGGGCAGCAGGGGTTTGGGTTTCTTGTAGGGATACGttt                                                                         >  2:1530659/1‑151 (MQ=255)
                                                                            ggttggttTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGtttt                                                                        <  1:1408800/151‑1 (MQ=255)
                                                                              ttggttTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCCGGCCGGCGTACCCAGGGGTCGCCTTTTCTCGGCGGGGGGGGTTTTTAGTTTTCCGGGGGTCAGCAGGGCTTTGGTTTCCCGGGAGAGATACGtttttt                                                                      >  1:272693/1‑149 (MQ=255)
                                                                               tggttTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGCGGGGACGCATTTTTTTGGCGAGGAAGGTTTTTTGTTTTCCCGGGGTCAACAGGGCTTTGGGTTTCTGGTTGTGAAAAGGTttgtt                                                                     >  1:237172/1‑151 (MQ=255)
                                                                               tggttTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTttgt                                                                      <  1:512640/150‑1 (MQ=255)
                                                                                ggttTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTttgttg                                                                    <  1:957915/151‑1 (MQ=255)
                                                                                 gttTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCCGGCTGCAGGACCGAGGGGACGCATTTTTTCGGCGAGGGAGTTTTTTAGTTTTCCGGGAGGCAGCAGGGGTTTGGGTTTCTGGGAGAGATAAGTtttttt                                                                     >  2:1268733/1‑149 (MQ=255)
                                                                                     ccGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCCAGGGGACGCATTTTTTCCACGAGGAAGGTTTTCAGTTTTCCGGGGGGCAGCAGGGCTTTGGGTTTCTGGGAGAGATACGgttt                                                                        >  2:59778/1‑142 (MQ=255)
                                                                                      cGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCagag                                                              <  2:773725/151‑1 (MQ=255)
                                                                                       gCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGGAGGCTGCAGTACCGCGGGGAAGCATTTTTTCGCCGAGGGAGTTTTTCAGTTTGCCGGGGGGCAGGCGGGCGTTGGTTTCCTGGGTGGGAAACGTTTTTTTgg                                                                   >  1:1083323/1‑145 (MQ=255)
                                                                                         aGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTAACGAGGGGACGCATTTTTTCGACGAGGAAGTTTTTTAGTTTGCCGGGGGTCAGCAGGGGTTTGGGTTTCTGGGAGAGATACGTTTTTTTTGCAGAgcc                                                            >  2:384128/1‑150 (MQ=255)
                                                                                            cGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACGCATTTTTTCGACGAGGAAGGTTTTTAGTTTTCCGGGGGGCCGCAGGGGTTTTGGTTTCTGGGAGAGATACGTTTTGTTGGCAGAgccgcc                                                         >  2:1323540/1‑150 (MQ=255)
                                                                                               aCCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAgccgccgcc                                                      <  2:741448/150‑1 (MQ=255)
                                                                                                ccGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCtt                                                    <  2:71350/151‑1 (MQ=255)
                                                                                                  gCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTc                                                  <  1:547978/151‑1 (MQ=255)
                                                                                                    cAATCACAAACGCGATATGGTACGGCGGGCAGGCCGCAGTACCGAGGGGACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCCGGGCTTTGGTTTCCTGGGAGAGATACGTTTTTTTGGCAGAGCCGCCGCCTTTcgc                                                >  2:682227/1‑151 (MQ=255)
                                                                                                    cAATCACAAACGCGATATGGTACGGCGGGCAGGCCGCAGTAACGAGGGGACGCATTTTCTCGGCGAGGAAGTTTTTTCGTTTGCCGGGAGGCAGCACGGGTTTGGGTTTCCGGTAGAGATACGTTTTTTTGGCACAGCCGCCCCCctt                                                   >  2:1479895/1‑148 (MQ=255)
                                                                                                         acaAACGCGATATGGTACGGCGGCCAAGCTGCAGTACCGGGGGGACCGATTTTTTCGGAGAGGGAGGTTTTTTGGTTGCCCGGGGGCGGCAGGGCTTTTGTTTTCTGGTCGAGATACGGTTTGTTTGGCGAACCGCCCCCCTTcgcaacgc                                           >  1:243836/1‑151 (MQ=255)
                                                                                                          caAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTcgcaacgca                                          <  2:208700/151‑1 (MQ=255)
                                                                                                            aaCGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCaaa                                        <  1:1501215/151‑1 (MQ=255)
                                                                                                                cgATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTAAGCATTTTTTCGGCGAGGAAGGTTTTTAGTTTGCCGGGGGGCAGCAGGGCTTTGGTTTCCTGGGAGAGATACGTTTTTTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAgg                                      >  1:548323/1‑149 (MQ=255)
                                                                                                                 gATATGGTACGGCGGGCAGGCTGCAGTACCGGGGGGACGCATTTTTTCGACGAGGGAGGTTTTCAGTTTGCCGGGGGGCAGCAGGGGTTTGGTTTTCTGGTAGAGATACGTTTTTTTGGCAGAGGCGCCGCCTTTCGCAACGCAAAggga                                    >  1:1274132/1‑148 (MQ=255)
                                                                                                                 gATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGGTTCCTGGGAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGaa                                    >  2:124022/1‑150 (MQ=255)
                                                                                                                      ggTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGt                               <  1:277164/150‑1 (MQ=255)
                                                                                                                         aCGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACt                            <  2:237197/150‑1 (MQ=255)
                                                                                                                           ggcggGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCAt                         >  1:874061/1‑151 (MQ=255)
                                                                                                                            gcggGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATc                        <  2:1523962/151‑1 (MQ=255)
                                                                                                                             cggGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCg                       >  2:900404/1‑151 (MQ=255)
                                                                                                                               ggCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGcc                     <  1:430696/151‑1 (MQ=255)
                                                                                                                                gCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCa                    <  2:1145489/151‑1 (MQ=255)
                                                                                                                                gCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCa                    <  2:263672/151‑1 (MQ=255)
                                                                                                                                   ggCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCt                 <  2:473205/151‑1 (MQ=255)
                                                                                                                                       gCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTAcc              <  2:677304/150‑1 (MQ=255)
                                                                                                                                       gCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTAcc              <  1:555593/150‑1 (MQ=255)
                                                                                                                                        cAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACcgc            <  1:1141454/151‑1 (MQ=255)
                                                                                                                                            aCCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTAc        >  2:547956/1‑151 (MQ=255)
                                                                                                                                             ccGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACa       <  2:1214355/151‑1 (MQ=255)
                                                                                                                                                aGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAGGt    >  2:663842/1‑151 (MQ=255)
                                                                                                                                                aGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAGGt    >  2:428034/1‑151 (MQ=255)
                                                                                                                                                 gggTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAGGt    <  1:764856/150‑1 (MQ=255)
                                                                                                                                                   gTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAGGTCg  >  2:842727/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAGGTCG  >  NC_000913/4346527‑4346823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑